LOCUS       sequence-46597-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Bocchi R, Egervari K, Carol-Perdiguer L, Viale B, Quairiaux C, De 
            Roo M, Boitard M, Oskouie S, Salmon P, Kiss JZ
  TITLE     Perturbed Wnt signaling leads to neuronal migration delay, altered 
            interhemispheric connections and impaired social behavior.
  JOURNAL   Nat Commun. 2017 Oct 27;8(1):1158. doi: 10.1038/s41467-017-01046-w.
  PUBMED    29079819
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-10-27"; volume: "8(1):1158. doi"; pages: " 
            10.1038/s41467-017-01046-w"
COMMENT     Sequence Label: pCLX-UBI-Tred
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     287..306
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    305..360
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     406..425
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     LTR             463..643
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     protein_bind    663..696
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cagctatgac tgggagtagt caggagagga ggaaaaatct ggctagtaaa acatgtaagg
       61 aaaattttag ggatgttaaa gaaaaaaata acacaaaaca aaatataaaa aaaatctaac
      121 ctcaagtcaa ggcttttcta tggaataagg aatggacagc agggggctgt ttcatatact
      181 gatgacctct ttatagccaa cctttgttca tggcagccag catatgggca tatgttgcca
      241 aactctaaac caaatactca ttctgatgtt ttaaatgatt tgccctccca tatgtccttc
      301 cgagtgagag acacaaaaaa ttccaacaca ctattgcaat gaaaataaat ttcctttatt
      361 agccagaagt cagatgctca aggggcttca tgatgtcccc ataatttttg gcagagggaa
      421 aaagatctgc tagctcgaca cactggcgat tcaactagat gctgctagag attttccaca
      481 ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga cgggcacaca
      541 ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc cctagttagc
      601 cagagagctc ccaggctcag atctggtcta accagagaga cccagtacaa gcaaaaagca
      661 gcataacttc gtataatgta tgctatacga agttattccc atggtgaatt gatcccatct
      721 tgtcttcgtt gggagtgaat tagcccttcc agtcccccct tttcttttaa aaagtggcta
      781 agatctacag ctgccttgta agtcattggt
//
