LOCUS       sequence-465991-        1825 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Term_BBa-B0015_PartType10.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK151 [Term_BBa-B0015_PartType10]
FEATURES             Location/Qualifiers
     source          1..1825
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      628..699
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      715..742
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        803..894
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(895..963,964..1755)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1759..1798
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctatctc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
      661 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
      721 ccttcgggtg ggcctttctg cgtttatata catgagacca gagtagcccg cctaatgagc
      781 gggctttttt ttaattcccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      841 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagc
      901 attcagcatt ttcgtgtggc gctgattccg ttttttgcgg cgttttgcct gccggtgttt
      961 gcgcatccgg aaaccctggt gaaagtgaaa gatgcggaag atcaactggg tgcgcgcgtg
     1021 ggctatattg aactggatct gaacagcggc aaaattctgg aatcttttcg tccggaagaa
     1081 cgttttccga tgatgagcac ctttaaagtg ctgctgtgcg gtgcggttct gagccgtgtg
     1141 gatgcgggcc aggaacaact gggccgtcgt attcattata gccagaacga tctggtggaa
     1201 tatagcccgg tgaccgaaaa acatctgacc gatggcatga ccgtgcgtga actgtgcagc
     1261 gcggcgatta ccatgagcga taacaccgcg gcgaacctgc tgctgacgac cattggcggt
     1321 ccgaaagaac tgaccgcgtt tctgcataac atgggcgatc atgtgacccg tctggatcgt
     1381 tgggaaccgg aactgaacga agcgattccg aacgatgaac gtgataccac catgccggca
     1441 gcaatggcga ccaccctgcg taaactgctg acgggtgagc tgctgaccct ggcaagccgc
     1501 cagcaactga ttgattggat ggaagcggat aaagtggcgg gtccgctgct gcgtagcgcg
     1561 ctgccggctg gctggtttat tgcggataaa agcggtgcgg gcgaacgtgg cagccgtggc
     1621 attattgcgg cgctgggccc ggatggtaaa ccgagccgta ttgtggtgat ttataccacc
     1681 ggcagccagg cgacgatgga tgaacgtaac cgtcagattg cggaaattgg cgcgagcctg
     1741 attaaacatt ggtaaaccga tacaattaaa ggctcctttt ggagcctttt tttttggacg
     1801 acccttgtca gatcaaagga tcttc
//
