LOCUS       sequence-466007-        2320 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressed synthetic RNA target for RENDR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Gambill L, Staubus A, Mo KW, Ameruoso A, Chappell J
  TITLE     A split ribozyme that links detection of a native RNA to orthogonal 
            protein outputs.
  JOURNAL   Nat Commun. 2023 Feb 1;14(1):543. doi: 10.1038/s41467-023-36073-3.
  PUBMED    36725852
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-02-1"; volume: "14(1):543. doi"; pages: " 
            10.1038/s41467-023-36073-3"
COMMENT     Sequence Label: pJEC780
FEATURES             Location/Qualifiers
     source          1..2320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     354..373
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(592..696)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     764..782
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(786..829)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     promoter        847..881
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     primer_bind     complement(1121..1138)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1292..1880)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1372..1391)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2051..2320,1..522,523..591))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
       61 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
      121 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
      181 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
      241 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
      301 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
      361 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
      421 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
      481 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
      541 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
      601 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
      661 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
      721 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
      781 gcagaatttc agataaaaaa aatccttagc tttcgctaag gatgatttct ggaattctaa
      841 agatctttga cagctagctc agtcctaggt ataatactag tacctccact caacgatgtg
      901 gggacgccgt tgcaacttcg aggacctaat gtgaccgacc tagattcgtc attgtgggca
      961 gaatgaagta ttggcagaca ttgagtgccg aacaagacct gacctaacgg taagagagtc
     1021 tcataatacg tccggcctct tgcgcaggtt atattatgca aagcccgccg aaaggcgggc
     1081 ttttttttgg atccttactc gagtctagac tgcaggcttc ctcgctcact gactcgctgc
     1141 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     1201 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     1261 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag gctccgcccc cctgacgagc
     1321 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     1381 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     1441 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     1501 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     1561 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     1621 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     1681 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     1741 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     1801 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     1861 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     1921 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     1981 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     2041 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     2101 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     2161 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     2221 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     2281 cctccatcca gtctattaat tgttgccggg aagctagagt
//
