LOCUS       sequence-466008-       11088 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Sr62NLRa in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11088)
  AUTHORS   Chen R, Chen J, Outram MA, Powell OR, Arndell T, Gajendiran K, Wang 
            YL, Ayliffe MA, Blundell C, Figueroa M, Sperschneider J, Vanhercke 
            T, Tang D, Xu Y, Zhong G, Gardener C, Yu G, Gourdoupis S, Jaremko Ł,
            Matny O, Steffenson BJ, Boshoff WHP, Meyer WB, Arold ST, Dodds PN, 
            Wulff BBH
  TITLE     A wheat tandem kinase sensor activates an NLR helper to trigger 
            immunity
  JOURNAL   bioRxiv 2024.08.30.610287
REFERENCE   2  (bases 1 to 11088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.08.30.610287"
COMMENT     Sequence Label: pGADT7_Sr62NLRa
FEATURES             Location/Qualifiers
     source          1..11088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(396..1187,1188..1256))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1019..1038
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1257..1361)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1429..1447
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1485..1507)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1607..1626
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      1643..2807
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        3581..4285
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             4331..4351
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4367..4708
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     primer_bind     4714..4733
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4714..4732
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4739..4748
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4751..4777
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      8326..8513
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(8381..8405)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(8630..9724)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIEATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(9725..10130)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(10172..10188)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10172..10188)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10185..10207)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10196..10212
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10220..10226,10227..10244,10245..10250))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10265..10286
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    10341..10374
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    10452..10485
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(10554..10571)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(10725..11088,1..225))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10805..10824)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
       61 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      121 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      181 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
      241 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
      301 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
      361 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
      421 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
      481 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
      541 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
      601 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
      661 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
      721 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
      781 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
      841 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
      901 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
      961 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     1021 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     1081 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     1141 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     1201 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     1261 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     1321 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     1381 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     1441 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     1501 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     1561 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     1621 attgtactga gagtgcacca taacgcattt aagcataaac acgcactatg ccgttcttct
     1681 catgtatata tatatacagg caacacgcag atataggtgc gacgtgaaca gtgagctgta
     1741 tgtgcgcagc tcgcgttgca ttttcggaag cgctcgtttt cggaaacgct ttgaagttcc
     1801 tattccgaag ttcctattct ctagctagaa agtataggaa cttcagagcg cttttgaaaa
     1861 ccaaaagcgc tctgaagacg cactttcaaa aaaccaaaaa cgcaccggac tgtaacgagc
     1921 tactaaaata ttgcgaatac cgcttccaca aacattgctc aaaagtatct ctttgctata
     1981 tatctctgtg ctatatccct atataaccta cccatccacc tttcgctcct tgaacttgca
     2041 tctaaactcg acctctacat tttttatgtt tatctctagt attactcttt agacaaaaaa
     2101 attgtagtaa gaactattca tagagtgaat cgaaaacaat acgaaaatgt aaacatttcc
     2161 tatacgtagt atatagagac aaaatagaag aaaccgttca taattttctg accaatgaag
     2221 aatcatcaac gctatcactt tctgttcaca aagtatgcgc aatccacatc ggtatagaat
     2281 ataatcgggg atgcctttat cttgaaaaaa tgcacccgca gcttcgctag taatcagtaa
     2341 acgcgggaag tggagtcagg ctttttttat ggaagagaaa atagacacca aagtagcctt
     2401 cttctaacct taacggacct acagtgcaaa aagttatcaa gagactgcat tatagagcgc
     2461 acaaaggaga aaaaaagtaa tctaagatgc tttgttagaa aaatagcgct ctcgggatgc
     2521 atttttgtag aacaaaaaag aagtatagat tctttgttgg taaaatagcg ctctcgcgtt
     2581 gcatttctgt tctgtaaaaa tgcagctcag attctttgtt tgaaaaatta gcgctctcgc
     2641 gttgcatttt tgttttacaa aaatgaagca cagattcttc gttggtaaaa tagcgctttc
     2701 gcgttgcatt tctgttctgt aaaaatgcag ctcagattct ttgtttgaaa aattagcgct
     2761 ctcgcgttgc atttttgttc tacaaaatga agcacagatg cttcgttgct tgcatgcctg
     2821 caggtcgaga tccgggatcg aagaaatgat ggtaaatgaa ataggaaatc aaggagcatg
     2881 aaggcaaaag acaaatataa gggtcgaacg aaaaataaag tgaaaagtgt tgatatgatg
     2941 tatttggctt tgcggcgccg aaaaaacgag tttacgcaat tgcacaatca tgctgactct
     3001 gtggcggacc cgcgctcttg ccggcccggc gataacgctg ggcgtgaggc tgtgcccggc
     3061 ggagtttttt gcgcctgcat tttccaaggt ttaccctgcg ctaaggggcg agattggaga
     3121 agcaataaga atgccggttg gggttgcgat gatgacgacc acgacaactg gtgtcattat
     3181 ttaagttgcc gaaagaacct gagtgcattt gcaacatgag tatactagaa gaatgagcca
     3241 agacttgcga gacgcgagtt tgccggtggt gcgaacaata gagcgaccat gaccttgaag
     3301 gtgagacgcg cataaccgct agagtacttt gaagaggaaa cagcaatagg gttgctacca
     3361 gtataaatag acaggtacat acaacactgg aaatggttgt ctgtttgagt acgctttcaa
     3421 ttcatttggg tgtgcacttt attatgttac aatatggaag ggaactttac acttctccta
     3481 tgcacatata ttaattaaag tccaatgcta gtagagaagg ggggtaacac ccctccgcgc
     3541 tcttttccga tttttttcta aaccgtggaa tatttcggat atccttttgt tgtttccggg
     3601 tgtacaatat ggacttcctc ttttctggca accaaaccca tacatcggga ttcctataat
     3661 accttcgttg gtctccctaa catgtaggtg gcggagggga gatatacaat agaacagata
     3721 ccagacaaga cataatgggc taaacaagac tacaccaatt acactgcctc attgatggtg
     3781 gtacataacg aactaatact gtagccctag acttgatagc catcatcata tcgaagtttc
     3841 actacccttt ttccatttgc catctattga agtaataata ggcgcatgca acttcttttc
     3901 tttttttttc ttttctctct cccccgttgt tgtctcacca tatccgcaat gacaaaaaaa
     3961 tgatggaaga cactaaagga aaaaattaac gacaaagaca gcaccaacag atgtcgttgt
     4021 tccagagctg atgaggggta tctcgaagca cacgaaactt tttccttcct tcattcacgc
     4081 acactactct ctaatgagca acggtatacg gccttccttc cagttacttg aatttgaaat
     4141 aaaaaaaagt ttgctgtctt gctatcaagt ataaatagac ctgcaattat taatcttttg
     4201 tttcctcgtc attgttctcg ttccctttct tccttgtttc tttttctgca caatatttca
     4261 agctatacca agcatacaat caactccaag ctttgcaaag atggataaag cggaattaat
     4321 tcccgagcct ccaaaaaaga agagaaaggt cgaattgggt accgccgcca attttaatca
     4381 aagtgggaat attgctgata gctcattgtc cttcactttc actaacagta gcaacggtcc
     4441 gaacctcata acaactcaaa caaattctca agcgctttca caaccaattg cctcctctaa
     4501 cgttcatgat aacttcatga ataatgaaat cacggctagt aaaattgatg atggtaataa
     4561 ttcaaaacca ctgtcacctg gttggacgga ccaaactgcg tataacgcgt ttggaatcac
     4621 tacagggatg tttaatacca ctacaatgga tgatgtatat aactatctat tcgatgatga
     4681 agatacccca ccaaacccaa aaaaagagat ctttaatacg actcactata gggcgagcgc
     4741 cgccatggag tacccatacg acgtaccaga ttacgctcat atggccatgg aggccagtga
     4801 attcatggag gagatcgtgc ttggactgtc caagacggtg gtggagggga cgctggtcaa
     4861 ggtgaaggcg gcgattgacg aggagtcgag gctgaagctc gcggtgcaga gcgacctggt
     4921 gttcatcacg ggggaattcg agatgatgca gtcgttcctc aacgtcgccg acgcggagcg
     4981 catcaagaac aacgccgtca agacgtgggt gaggcagctc cgcgacctgg cctacgacac
     5041 cgaagactgc atcgagctgg tcgtccacct ggatcccaag cccagatggt ggcgccgcct
     5101 gctggtcctg ccttgcctgc cggcggtgtc gctgcccatg gatgacgcgg cggcggagat
     5161 aaaggagctc aagggcaggg tggagttcgt gagccagcgg aatatgcgct acaacctcat
     5221 caccgacttc ggcggcgcca agtccgccgc gcagcagcag cttgaccggg ccgccggagc
     5281 tctcgacatc gccgtggagg cggcggcgag gaaagggcgt agcttggtgg atctgaccga
     5341 gttgatcccc aaaatggaag accgccctga gctcggcgtg atctcggtgt ggggcacagg
     5401 cggcgacctt ggggtggcgt ccatcgtcag gaaggcctac gacgaccctg aaatacgcaa
     5461 gaacttccat agtcgtggct gggccaagtt gacccaccct ttcgatccgc ggaagatcct
     5521 ccggagcttg ctcattcagt tctgcactaa cactgctgct ccacagaagc aaggggagac
     5581 tctggatgta gattcgttgc tgaagatgga gaagacagtg gtggaagaag gggagctcgt
     5641 caagcagttt gtgagtcatg tgagcaccca caggttcctc gtcgtcctcg aaggcgtgtc
     5701 aaccatggcg gaatgggatg ccctccggat gtacctgcca gacatgggta atgggagtca
     5761 gatcatcgta tccacacagc agttcgacat cgcgagcttg tgcaccgggc aactgcacaa
     5821 agtttcagag ttgagaaaat tcacacctga ccactctgtt tgtgtcttct tcaaggaggt
     5881 ttctggaggt gatgatcctg gaaatgcaat gctggaggga gatgacaaag ctgaacttat
     5941 tgggcgtgac acagaagagg aggagcttat ggagcagatt gatgaggttt cccgtgtgat
     6001 ctcggtatgg gggattgctg gtgtaggaaa atcggcactt gtcagaagtg cctactgcaa
     6061 gtatctgcag gaccgcccca actttgctgg gttaaatctg acctacatac acgggcgcgt
     6121 gaccgtgccc catcccttta atctgaggga gttctgccgc agcttgctcc tgaacttgtc
     6181 ctctgagcct gctaaaacca aagaggacgc cgtcgctgaa ctggcaaaca tgaccaatcc
     6241 aactgatgtc tgccgtaagc ttcttagcga cgatcaacaa cagaagcagt gccttattgt
     6301 tattgatgac ctgcagtcca ctgaagaatg ggacagaata aaacaggcct tgtcatttgg
     6361 taaagaatgt tgtatcatag ttgttacgaa tgattcaagt gttgccaaat attgttcagg
     6421 gcaagatcag aaactcatgt tcaacgtcaa aggtcttggt gacgagcatg cccttgacct
     6481 cttcaacacg gtatatgacg gcatgcctaa ccacatcaca cgggatgagg acatgatcaa
     6541 tcagtcgaag ctaatgttaa acaaatgcgg cggactaccc aaggtgatag ttgcaattgg
     6601 tcgtttcttg gccaagagtc tgaattggga ggccatgaat actaacttta ttcaccagtt
     6661 ggaaaacaac ccagagctgg ctagtataca tagcctattt agttggctgg actcctactt
     6721 ccacaactgt cctgatgaac tcaagccatg catcttctat ttgtcaatct tccctcgacg
     6781 ccatggcatt cgtcggaggc gtctggtgag gcggtggatc gccgagggct actcaaggga
     6841 caccgacggg aatctcgcgg aagtgaacgg ggaaaattac ttctccaggc ttgtcaacct
     6901 cagcatgctc acagatgctg agcgtggtgt caacgcggct gggacaggga ggaaaatggc
     6961 catgtgcaac atcaacgatt tcttccggga gtacattgtg tcgcggcgaa tggaggagaa
     7021 ccatgtgttt gcactggagg ggaggtgcag ccagaccaca cggcgcactg ggcggcacct
     7081 ggtcatcaac caaagctggg acggagatga gaacgtgttc aacaggatcg agttctcgcg
     7141 actgcggtcg gtgacagtgt tcggaccctg gaagccattc ctggcctccg acaagatgag
     7201 ggtgctccgc gtgcttgatc tggaaggcac tgaaggtctg acagacgacg acatcaagag
     7261 catcgtcaaa cggctgcctc gactgaagtt cctctccctg cgaaaatgca aaaacatctt
     7321 tcggctgccg aagtccctgg gtcgcctgag gcagctccag accctggatg tgaggcacac
     7381 tgccatagcc cagctgccag cgaccgtcgt gaagctacag aagttgcagt atgttcgtgc
     7441 cggtaacaca ccagaggaat atgccggcag cgacagcacg gaatctagca cctcggtacc
     7501 cgcctgtttc tcatgtggcc ctgttggcgg tcacattgtc ggcgttgagg tgcctcgcgg
     7561 ggtcgagaat ctgaccaacc tgcacacact gggtgtcatc aaagccactg tggcgggact
     7621 caaggagctc aaaaagctta cccagctgcg caagctcggt gtgtctggca tcaaccggag
     7681 aaaccacaaa gagctctgtg ccgtcgtctc cggacatggt catctggaat ccctgtcgat
     7741 atggcttgac aaggacaccg aggggattgc tgacccttgt tcgccgagca atgatgactt
     7801 caagcctcca gagaagctac ggaggctcaa actccacggg cacatcggca atttgccagc
     7861 atgggttagc actctcacca tgctggattt ggtggaagta tccaagtctg ctgctcaaga
     7921 tccatgagga tccatcgagc tcgagctgca gatgaatcgt agatactgaa aaaccccgca
     7981 agttcacttc aactgtgcat cgtgcaccat ctcaatttct ttcatttata catcgttttg
     8041 ccttctttta tgtaactata ctcctctaag tttcaatctt ggccatgtaa cctctgatct
     8101 atagaatttt ttaaatgact agaattaatg cccatctttt ttttggacct aaattcttca
     8161 tgaaaatata ttacgagggc ttattcagaa gctttggact tcttcgccag aggtttggtc
     8221 aagtctccaa tcaaggttgt cggcttgtct accttgccag aaatttacga aaagatggaa
     8281 aagggtcaaa tcgttggtag atacgttgtt gacacttcta aataagcgaa tttcttatga
     8341 tttatgattt ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt
     8401 gactcttagg ttttaaaacg aaaattctta ttcttgagta actctttcct gtaggtcagg
     8461 ttgctttctc aggtatagca tgaggtcgct cttattgacc acacctctac cggccggtcg
     8521 aaattcccct accctatgaa catattccat tttgtaattt cgtgtcgttt ctattatgaa
     8581 tttcatttat aaagtttatg tacaaatatc ataaaaaaag agaatctttt taagcaagga
     8641 ttttcttaac ttcttcggcg acagcatcac cgacttcggt ggtactgttg gaaccaccta
     8701 aatcaccagt tctgatacct gcatccaaaa cctttttaac tgcatcttca atggccttac
     8761 cttcttcagg caagttcaat gacaatttca acatcattgc agcagacaag atagtggcga
     8821 tagggttgac cttattcttt ggcaaatctg gagcagaacc gtggcatggt tcgtacaaac
     8881 caaatgcggt gttcttgtct ggcaaagagg ccaaggacgc agatggcaac aaacccaagg
     8941 aacctgggat aacggaggct tcatcggaga tgatatcacc aaacatgttg ctggtgatta
     9001 taataccatt taggtgggtt gggttcttaa ctaggatcat ggcggcagaa tcaatcaatt
     9061 gatgttgaac cttcaatgta ggaaattcgt tcttgatggt ttcctccaca gtttttctcc
     9121 ataatcttga agaggccaaa acattagctt tatccaagga ccaaataggc aatggtggct
     9181 catgttgtag ggccatgaaa gcggccattc ttgtgattct ttgcacttct ggaacggtgt
     9241 attgttcact atcccaagcg acaccatcac catcgtcttc ctttctctta ccaaagtaaa
     9301 tacctcccac taattctctg acaacaacga agtcagtacc tttagcaaat tgtggcttga
     9361 ttggagataa gtctaaaaga gagtcggatg caaagttaca tggtcttaag ttggcgtaca
     9421 attgaagttc tttacggatt tttagtaaac cttgttcagg tctaacacta cctgtacccc
     9481 atttaggacc acccacagca cctaacaaaa cggcatcagc cttcttggag gcttccagcg
     9541 cctcatctgg aagtgggaca cctgtagctt cgatagcagc accaccaatt aaatgatttt
     9601 cgaaatcgaa cttgacattg gaacgaacat cagaaatagc tttaagaacc ttaatggctt
     9661 cggctgtgat ttcttgacca acgtggtcac ctggcaaaac gacgatcttc ttaggggcag
     9721 acattagaat ggtatatcct tgaaatatat atatatattg ctgaaatgta aaaggtaaga
     9781 aaagttagaa agtaagacga ttgctaacca cctattggaa aaaacaatag gtccttaaat
     9841 aatattgtca acttcaagta ttgtgatgca agcatttagt catgaacgct tctctattct
     9901 atatgaaaag ccggttccgg cgctctcacc tttccttttt ctcccaattt ttcagttgaa
     9961 aaaggtatat gcgtcaggcg acctctgaaa ttaacaaaaa atttccagtc atcgaatttg
    10021 attctgtgcg atagcgcccc tgtgtgttct cgttatgttg aggaaaaaaa taatggttgc
    10081 taagagattc gaactcttgc atcttacgat acctgagtat tcccacagtt gggggatctc
    10141 gactctagct agaggatcaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt
    10201 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
    10261 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
    10321 gaaacctgtc gtgccagctg ataacttcgt ataatgtatg ctatacgaag ttattaggtc
    10381 tgaagaggag tttacgtcca gccaagctag cttggctgca ggtcgagcgg ccgcgatccg
    10441 gaacccttaa tataacttcg tataatgtat gctatacgaa gttatcagct gcattaatga
    10501 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
    10561 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
    10621 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
    10681 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
    10741 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
    10801 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
    10861 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
    10921 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    10981 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    11041 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaac
//
