LOCUS       sequence-46601-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  TITLE     pLS/-11 combination of pLS1 encoding lactose repressor with deletion
            of 11 C-terminal amino acids
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLS1/-11
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    529..550
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(565..570,571..588,589..595)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    603..619
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     608..630
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     627..643
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     627..643
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             639..863
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWL"
     primer_bind     complement(659..676)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(659..675)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(668..690)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac tcgcaatcaa
       61 attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc
      121 atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa cgatcagatg
      181 gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc ggatatctcg
      241 gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt aaccaccatc
      301 aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag
      361 ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc
      421 ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
      481 taacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
      541 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
      601 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acggattcac
      661 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
      721 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
      781 cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt tgcctggttt ccggcaccag
      841 aagcggtgcc ggaaagctgg ctgga
//
