LOCUS       sequence-466010-       14304 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Eggerthella lenta A2 (+)-Eddh without accessory genes 
            (inactive) in Gordonibacter urolithinfaciens DSM 27213.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14304)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 14304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
COMMENT     Sequence Label: pXD80EN2-Elen0501-(+)-Eddh-noAccs(ElA2)
FEATURES             Location/Qualifiers
     source          1..14304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..813)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     1300..1318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1475..1494
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             complement(1503..1514)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      7928..7955
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      8087..8114
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(8546..9397)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(9384..10223)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(10734..11705)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            12944..13031
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      13373..13767
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(13930..13973)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     oriT            14069..14178
                     /note="incP origin of transfer"
ORIGIN
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagatctat
     1501 tcgcgtcctt cgatcacgtc gaggaactcc tgcttgccgt ggacgtccat cttttggtag
     1561 atgtggcgca ggtgggtggt caccgtgccg cgcgacaggt agaggtcttc ctggatgcgg
     1621 gtgctcgagc ggcccttcgc gaacaggatc atgatctcgg tttccttcgg cgacagaccg
     1681 tattccccgg ccacgtcttt gcagcggtct tggaaacgct ggcgcctcgg ctcggcgccg
     1741 cccttgtcct catcgccctt cgccagcgcg atgaggtcgt tctcgttgaa cacgaacatg
     1801 tacgagatga ggatggccag cgtggccagc agcgcgatca ggctgaggct ctgcggcgag
     1861 aacggcgcga acgtgcacac gaactgcccc agcgaggaac cgagcagcac gccgagcgtc
     1921 accatgcccc agccgatgcc gaacaccatg acgcccgaca gccggtacgt gaacgagatg
     1981 tcggcgagca gtatccagat gagcacgttg aacgtgccgt agccggccag cgcgatggtg
     2041 ctctcgatct cggaaaagcc ggtgaacacg ggcagcagca tgaagaacac ggccatgacc
     2101 agcatcaccg agcggtacac gggacgtagg cccgttttgc tggagagcag cacgcagccg
     2161 tagacgatgg cgatggtgag caggctcgac caaaggagcg ggaagcggta gaagttctcg
     2221 acgctggcct cgttcgcggt gatgaacacc gcgcgcacca cgccgtcggc catgccgatg
     2281 aggcaggcgc acacgccgat cttccacgag aacttcccca ggtggatctc gaacggctgc
     2341 acgaccggct cggctttcga cgaccacgtc ctcagcttca cgaacagaat ccaacctgac
     2401 gcggcgggca gcagcgcgca gaccacgcac gctccgacgg gcggcaaggt cagaacggcg
     2461 gggaagaggg ccgcggcgat caggaaggcg agcggcgctt cgaacgacgt gcggcgggga
     2521 tcgacattgc ggtacagctc gccgtacccc aggtcgatga gccccgatcc ggcgccggtc
     2581 gataccgagc cgaacgcgca cagcgccagc gccggtgcgc tcggcagggt ggcgcaggcg
     2641 agggccagcg tgccgacggt ggtgagggcc gggccgagga agcggaacgc gcgatggccg
     2701 atgagcgccg tgaagcgccg gtggaagatc gcgcttgcga acagggtcgc gcacagcacg
     2761 agcgtcgatc ccaagtaagc cgcgccgggc agcggctcgc cgttccacgt gatggagctg
     2821 gtggcggcgc ctaggaacat gaggtacacc cacgcgcgcg ccagggcgaa gccgagcatg
     2881 cgcaggttca acaggctgcg cggcgccgtc tcgcgcaggc gatcccgggc ggtggtcagc
     2941 ggtttcggca tgtgcgtcac gagaattccc cctcaagatc tcgatcccct gagcctcatt
     3001 atagcgtgcg cgatccgtcc ggctcacgtg gcccgatagg ttgatgcgtg acgtcatgct
     3061 ttgctcatca ggtgtttctc caaaatcatg ggaaatgcat gatgggaaag cctccgattc
     3121 gcccgatttt ttgcagaaac gcatgatgcg cggacgtccc ctgccgcgca tgctcgaaca
     3181 aagcggcggg cgttctcgac gagccgtcgc aaggacgaga cggagaagga ggaggaatgg
     3241 gaaagctcaa tctgacgcgc cgtgcgttca cgaagctcac ggccgtgacg ggtgcggcgt
     3301 tggcctgcgc ggcagcggtc gccccgaacg cggcgctggc cgaagatgcc ggagcggccg
     3361 cgcgcggcga cgacgtgaaa cgcgtgcgca cgtgctgccg cggctgcgga aagatggagt
     3421 gcggcgtgtg ggtgaccgtg caaaacggcc gcgctatcaa ggtggagggc gaccagtcgt
     3481 cattccagtc gagcggcaac tgctgcggca agtcgcagtc gtccatccag gcggcgtacc
     3541 accccgaccg catctaccat cccatgaagc gcacgaatcc caagggcgag gatcccggct
     3601 ggcagcgcat cacgtgggac gaggcgatgg agatcgtcgg caccaagttc aacgagctga
     3661 tggaccgcta cggcggacag tgcatcttca acatggcggg cacgtcgcgc cagtgggtgt
     3721 acgggcccta cgcgttctac aagtggctgt tcgacacgcc gaacgcgcac gtggcgtcgg
     3781 aaatctgcaa ggggccgcgc cgtttgatgg ggtggatcag ctcggtcgac ggcgcgccgt
     3841 ggatggcgct gcgcgacggg ccgcgcgtgt acgtgcagtg ggggaccgcg cccgaaaact
     3901 cgaactacga tgacagctgc cgcaacctcg tggacaagat gaccgaggcc gacgtgcaca
     3961 tctgcatcga tccgcgcctg tccggctcgg gcaaggaggc cgactactgg ctgaacctgc
     4021 gtcccggctc cgacggcgcg ctggcgctgt gctggcagca cctcgtcatc aagaacgacc
     4081 tggtggactg ggagttcgtg aagcgctgga cgaactcgtc gttcctcgtg gtggaggacc
     4141 gggagcccac gggcggccgc tacatcgacc tgtccacgcc gctcaacaac gccggcatcc
     4201 ccgccgacgt cgtgggcacg aagctcaaga cgcgcctgct gaaggaaagc gatgtggtgg
     4261 agggcggcag cccgcgcaag ttctacgcgt ggaacaaact ggccaacgac ggcgccggcg
     4321 gcttggtgat gtgggacgtc gacaccacgc aatgggaggg ctgcaaccac gtggccccca
     4381 cgcgcgacca gatggaggtc gtgtacaagg gcacgtcgca ggagggctat ttgccgccgc
     4441 tgtcctatca cgagctggag gaagccggca tcgacctgga tatgcgtggg acgcatgagg
     4501 tggagctgct cgacggttcg aagcacacgg cgaagccggt gtgggcgtac ctcgaggaat
     4561 cggtggcgga ctgcacgccc gcgtggtgct ccgagatcac gggcctcgat cctgcgctca
     4621 tcgaagaggc gtgcctcgtg tgggccacgc gtcccgaggg gcaggattac ggcaacggcg
     4681 gtatccacct gaacctcgcg cccgaccaga tcggcaactg cacgcagacg gtgcgcgcgg
     4741 tgctgcacct catctacatg accggcaact tcgacacgcc cgccggcaac cgcggcctca
     4801 cgcgctcgcc catcgacgag caggccacgg ccgcgcccgg ctcgaacatg ccgcaggaag
     4861 tgaaggccca gctgatcgcc ttgggcgaga tccccgtgga aggcgtcacg cccgatcccc
     4921 tcaacgtgcc cgaccgctac gacacgctgt cgaacatggt gggcgccgac gagttcccca
     4981 tcacggcgta ctacaacgag tgggccgacg cgacgcgcat ctgggacgcc tgcctcaccg
     5041 gcgagccgta ccccgtgcgc ggcggcatca acgagtccgg ctcgttcatg aacatgtcga
     5101 acgcgaacct ggcctgggag gcgttgcagt cgctcgattt ctgggtggac atcaacatgt
     5161 tccaccatcc cggcaccgag atggccgaca tcctgctgcc gtgccagcat tggctggaga
     5221 tcaacaacat ccgcgtgtcg cagggcgcgt ccggcggtat cggcgccacc atccgcgcgg
     5281 tcgagccgcc cagcgacacg aagttcgact acgacatcaa ccgcctgctg ttcgacgccg
     5341 tgggcggccc gaacggaacc tggaccaaca tcgcgggcga cgcgcccggc ggctaccacg
     5401 tggacgagcg cttggaggac tggttccaga acaactcgaa gaccaatccc aaggtgaaat
     5461 ggcagcattg ggacgacttc gtggaggact ttcaggagaa cggctggatc aacgccaagg
     5521 agatcgagcc cgaccgctgg ggcacgtacc gccgcttcga gaccggctgg atgcgcatcg
     5581 gcaaggacgc gtgcaccggc tccacgttca gcgcggcgtt cgacgacgcc ggcaatccgg
     5641 tgaacaactt cggctgcccc acgccgacgg gcctcgtgga attttggccg ctcgtgttcg
     5701 agacgtactg cgtggacaag gcgaacgagt tcaaccccgg caagttcgac ctggtgcacg
     5761 agatgatgcc gcactacgac gagccgaaat cgggccccaa gggcgacgtg gacatgaacg
     5821 agtatcccat tatcctcacc accggccgcc gcatacccgt gtacttccat tccgagcacc
     5881 ggcagctgcc gtggtgtcgc gagctgtggc cggcaccgcg tttggaggtg aaccccgaag
     5941 acgctgccga gctggggctc gagcagggcg attgggcctg gatcgagacg gagtggggca
     6001 aggtgcgcca gtgcgtcgat ttgtactacg gcatcgcgaa ggggtgggcg aacgccgagc
     6061 acgcctggtg gttccccgag ctgcccgcgc cgacgcacgg gttcgagctg tcgaacatcg
     6121 agtgcatctg gaacccctac ggccaggatc cgttcatcgg gtcgtcccac atgcgcggcg
     6181 tgcccgtgaa gatatacaag gccacgcccg agaactgccc cgacggcaag gtcatcccct
     6241 gcgcgcccga ggacggcacc gagatcatct acgatgcctc cgacccgcgc ctgaaggaat
     6301 ggctgccgaa ctacaccatc cgagaggagg cgtaaagcca tgacccagta cgcgatcgtc
     6361 accgatttga accggtgcgt cggctgcctg gcctgcatgg ttgcctgcaa ggcggtcaac
     6421 aacgtgccgg tgggcaacta ttggaacaag gtgctgcgca tcggcccctc gctgaaagag
     6481 ggggccacgt cgtcgcacga cgtggagatg tactacctgc ccgtgcagtg ccagcactgc
     6541 gccgaccccg agtgcgtgaa ggtgtgcccg acgggcgcct cgcacaaact ggaggacggc
     6601 acggtgcaga tcgacaaggc gaagtgcatc ggctgccagt tctgcgcgat gtcgtgcccc
     6661 tacaacgtgc ggtacctgaa cgaggaagag ggcgtcgtgg agaagtgcac gctgtgcgcg
     6721 cagatcgtgg ggcagggcgg gttgccgcaa tgcgtcatcc agtgcggagg gcgcgcccgg
     6781 ttcttcggcg atctggacca ggggatcgag tcgttcgaag cccccgccgt cggctacgac
     6841 accgatcgca cctacgacgg cctgtatgca ggcaaccgcg tgacgctggg cgagttggcg
     6901 caggacttcg atgcgagcac cgatctgcat catctgccga acgtcggcaa cgatccgaac
     6961 ttcgcgttca tcctgcgcaa ccgcgactgg aagggtgatg aataatggaa ctccagtggc
     7021 ctttgatttt gttcaccacg ctggttgcgt ggagcgcggg gctgttcggg acgcaggcgc
     7081 tgatggcggt gttcggcgtc gggaagaagg cgcaggtgcc ggcgtgggtt gcgtcggccg
     7141 tcttgctggc ggccggcggg atcgcggtgt tcttccacct tgagcactgg gagcggatct
     7201 tcaacgggtt cgggcatttg acgagcggga tcacgcagga gctgatcgcg atcgtggtgc
     7261 ttgccgtggc cgccgtggcc tacctcgtgc tcatgcgcaa gtccgacgac ggcgcgagcg
     7321 tgccgaagtg gctggcgtgg ctgtccgtcg ccttgtcggt cgtgctggtc gccgtcatgg
     7381 cgcacagcta cacgatggcc gcccgccccg cctgggactc ggccctctgg atcctctacg
     7441 tcctcggcaa cgcctgcgtg ttgggccccg cgacgttcgc cctcctctcc accttggctg
     7501 cagggggacc ccgggaccaa cccgccgatc gcgcagccga cgccggggcc cccgcaggta
     7561 ggacggccct cgccggcgcc gccatcaacg ccctcgcaac cctcgccttc gccgccttcc
     7621 tccagctctc cgccggctcc ttcgccgacg tcggcctcta cttcgacccg acgcacccca
     7681 ccaaggccat ggccgacgca gccgcgacgg tcgcctccca ggccccgctg ctgtggctgg
     7741 gagccgtggc cgtgggcgcg atcgtgcccc tggccgccgc cttcatgggc aggcgcacgg
     7801 ggaactggag gctctgggcc ccggtcgcga tagccgccgc cctcgtcggc gccgtctgca
     7861 tgcgcgccgt cttctacaac ctggggctca gcgtgttcat gttctactag actagtcgac
     7921 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga gagaatataa
     7981 aaagccagat tattaatccg gcttttttat tatttaattc ctgttataaa aaaaggatca
     8041 attttgaact ctctcccaaa gttgatccct taacgatccc tgcaggagaa ggccatcctg
     8101 acggatggcc tttttgaagc tttctagaat cgggggcccc cggggggatt ctagaacaat
     8161 ggaagtcctc gtctcgccct cgaattagcc cgcttaatga gcgggctttt tttgaattaa
     8221 ttctcgcgag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     8281 ttaatgtaag ttagcgcgaa ttgcaagctg gccgacgcgc tgggctacgt cttgctggcg
     8341 ttcgggagca gaagagcata catctggaag caaagccagg aaagcggcct atggagctgt
     8401 gcggcagcgc tcagtaggca atttttcaaa atattgttaa gccttttctg agcatggtat
     8461 ttttcatggt attaccaatt agcaggaaaa taagccattg aatataaaag ataaaaatgt
     8521 cttgtttaca atagagtggg gggggtcagc ctgccgcctt gggccgggtg atgtcgtact
     8581 tgcccgccgc gaactcggtt accgtccagc ccagcgcgac cagctccggc aacgcctcgc
     8641 gcacccgctg gcggcgcttg cgcatggtcg aaccactggc ctctgacggc cagacatagc
     8701 cgcacaaggt atctatggaa gccttgccgg ttttgccggg gtcgatccag ccacacagcc
     8761 gctggtgcag caggcgggcg gtttcgctgt ccagcgcccg cacctcgtcc atgctgatgc
     8821 gcacatgctg gccgccaccc atgacggcct gcgcgatcaa ggggttcagg gccacgtaca
     8881 ggcgcccgtc cgcctcgtcg ctggcgtact ccgacagcag ccgaaacccc tgccgcttgc
     8941 ggccattctg ggcgatgatg gataccttcc aaaggcgctc gatgcagtcc tgtatgtgct
     9001 tgagcgcccc accactatcg acctctgccc cgatttcctt tgccagcgcc cgatagctac
     9061 ctttgaccac catggcatca gcggtgacgg cctcccactt gggttccagg aacagccgga
     9121 gctgccgtcc gccttcggtc ttgggttccg ggccaagcac taggccatta ggcccagcca
     9181 tggccaccag cccttgcagg atgcgcagat catcagcgcc cagcggctcc gggccgctga
     9241 actcgatccg cttgccgtcg ccgtagtcat acgtcacgtc cagcttgctg cgcttgcgct
     9301 cgccccgctt gagggcacgg aacaggccgg gggccagaca gtgcgccggg tcgtgtcgga
     9361 cgtggctgag gctgtgcttg ttcttaggct tcaccacggg gcaccccctt gctcttgcgc
     9421 tgcctctcca gcacggcggg cttgagcacc ccgccgtcat gccgcctgaa ccaccgatca
     9481 gcgaacggtg cgccatagtt ggccttgctc acaccgaagc ggacgaagaa ccggcgctgg
     9541 tcgtcgtcca caccccattc ctcggcctcg gcgctggtca tgctcgacag gtaggactgc
     9601 cagcggatgt tatcgaccag taccgagctg ccccggctgg cctgctgctg gtcgcctgcg
     9661 cccatcatgg ccgcgccctt gctggcatgg tgcaggaaca cgatagagca cccggtatcg
     9721 gcggcgatgg cctccatgcg accgatgacc tgggccatgg ggccgctggc gttttcttcc
     9781 tcgatgtgga accggcgcag cgtgtccagc accatcaggc ggcggccctc ggcggcgcgc
     9841 ttgaggccgt cgaaccactc cggggccatg atgttgggca ggctgccgat cagcggctgg
     9901 atcagcaggc cgtcagccac ggcttgccgt tcctcggcgc tgaggtgcgc cccaagggcg
     9961 tgcaggcggt gatgaatggc ggtgggcggg tcttcggcgg gcaggtagat caccgggccg
    10021 gtgggcagtt cgcccacctc cagcagatcc ggcccgcctg caatctgtgc ggccagttgc
    10081 agggccagca tggatttacc ggcaccaccg ggcgacacca gcgccccgac cgtaccggcc
    10141 accatgttgg gcaaaacgta gtccagcggt ggcggcgctg ctgcgaacgc ctccagaata
    10201 ttgataggct tatgggtagc cattgattgc ctcctttgca ggcagttggt ggttaggcgc
    10261 tggcggggtc actacccccg ccctgcgccg ctctgagttc ttccaggcac tcgcgcagcg
    10321 cctcgtattc gtcgtcggtc agccagaact tgcgctgacg catccctttg gccttcatgc
    10381 gctcggcata tcgcgcttgg cgtacagcgt cagggctggc cagcaggtcg ccggtctgct
    10441 tgtccttttg gtctttcata tcagtcaccg agaaacttgc cggggccgaa aggcttgtct
    10501 tcgcggaaca aggacaaggt gcagccgtca aggttaaggc tggccatatc agcgactgaa
    10561 aagcggccag cctcggcctt gtttgacgta taaccaaagc caccgggcaa ccaatagccc
    10621 ttgtcacttt tgatcaggta gaccgaccct gaagcgcttt tttcgtattc cataaaaccc
    10681 ccttctgtgc gtgagtactc atagtataac aggcgtgagt accaacgcaa gcactacatg
    10741 ctgaaatctg gcccgcccct gtccatgcct cgctggcggg gtgccggtgc ccgtgccagc
    10801 tcggcccgcg caagctggac gctgggcaga cccatgacct tgctgacggt gcgctcgatg
    10861 taatccgctt cgtggccggg cttgcgctct gccagcgctg ggctggcctc ggccatggcc
    10921 ttgccgattt cctcggcact gcggccccgg ctggccagct tctgcgcggc gataaagtcg
    10981 cacttgctga ggtcatcacc gaagcgcttg accagcccgg ccatctcgct gcggtactcg
    11041 tccagcgccg tgcgccggtg gcggctaagc tgccgctcgg acagttcgag gctggccagc
    11101 ctgcgggcct tctcctgctg ccgctgggcc tgctcgatct gctggccagc ctgctgcacc
    11161 agcgccgggc cagcggtggc ggtcttgccc ttggattcac gcagcagcac ccacggctga
    11221 taaccggcgc gggtggtgtg cttgtccttg cggttggtga agcccgccaa gcggccatag
    11281 tggcggctgt cggcgctggc cgggtcggcg tcgtactcgc tggccagcgt ccgggcaatc
    11341 tgcccccgaa gttcaccgcc tgcggcgtcg gccaccttga cccatgcctg atagttcttc
    11401 gggctggttt ccactaccag ggcaggctcc cggccctcgg ctttcatgtc atccaggtca
    11461 aactcgctga ggtcgtccac cagcaccaga ccatgccgct cctgctcggc gggcctgata
    11521 tacacgtcat tgccctgggc attcatccgc ttgagccatg gcgtgttctg gagcacttcg
    11581 gcggctgacc attcccggtt catcatctgg ccggtggtgg cgtccctgac gccgatatcg
    11641 aagcgctcac agcccatggc cttgagctgt cggcctatgg cctgcaaagt cctgtcgttc
    11701 ttcatcgggc caccaagcgc agccagatcg agccgtcctc ggttgtcagt ggcgtcaggt
    11761 cgagcaagag caacgatgcg atcagcagca ccaccgtagg catcatggaa gccagcatca
    11821 cggttagcca tagcttccag tgccaccccc gcgacgcgct ccgggcgctc tgcgcggcgc
    11881 tgctcacctc ggcggctacc tcccgcaact ctttggccag ctccacccat gccgcccctg
    11941 tctggcgctg ggctttcagc cactccgccg cctgcgcctc gctggcctgc tgggtctggc
    12001 tcatgacctg ccgggcttcg tcggccagtg tcgccatgct ctgggccagc ggttcgatct
    12061 gctccgctaa ctcgttgatg cctctggatt tcttcactct gtcgattgcg ttcatggtct
    12121 attgcctccc ggtattcctg taagtcgatg atctgggcgt tggcggtgtc gatgttcagg
    12181 gccacgtctg cccggtcggt gcggatgccc cggccttcca tctccaccac gttcggcccc
    12241 aggtgaacac cgggcaggcg ctcgatgccc tgcgcctcaa gtgttctgtg gtcaatgcgg
    12301 gcgtcgtggc ctgcccgctc taatgcccgg ttggcatggt cggcccatgc ctcgcgggtc
    12361 tgctcaagcc atgccttggg cttgagcgct tcggtcttct gtgccccgcc cttctccggg
    12421 gtcttgccgt tgtaccgctt gaaccactga gcggcgggcc gctcgatgcc gtcattgatc
    12481 cgctcggaga tcatcaggtg gcagtgcggg ttctcgccgc caccggcatg gatggccagc
    12541 gtatacggca ggcgctcggc accggtcagg tgctgggcga actcggacgc cagcgccttc
    12601 tgctggtcga gggtcagctc gaccggcagg gcaaattcga cctccttgaa cagccgccca
    12661 ttggcgcgtt catacaggtc ggcagcatcc cagtagtcgg cgggccgctc gacgaactcc
    12721 ggcatgtgcc cggattcggc gtgcaagact tcatccatgt cgcgggcata cttgccttcg
    12781 cgctggatga agtcggcctt ggccctggcc gattggccgc ccgacctgct gccggttttc
    12841 gccgtaaggt gataaatcgc catgctgcct cgctgttgct tttgcttttc ggctccatgc
    12901 aatggccctc ggagagcgca ccgcccgaag ggtggccgtt aggccagttt ctcgaagaga
    12961 aaccggtaag tgcgccctcc cctacaaagt agggtcggga ttgccgccgc tgtgcctcca
    13021 tgatagccta cgagacagca cattaacaat ggggtgtcaa gatggttaag gggagcaaca
    13081 aggcggcgga tcggctggcc aagctcgaag aacaacgagc gcgaatcaat gccgaaattc
    13141 agcgggtgcg ggcaagggaa cagcagcaag agcgcaagaa cgaaacaagg cgcaaggtgc
    13201 tggtgggggc catgattttg gccaaggtga acagcagcga gtggccggag gatcggctca
    13261 tggcggcaat ggatgcgtac cttgaacgcg accacgaccg cgccttgttc ggtctgccgc
    13321 cacgccagaa ggatgagccg ggctgaatga tcgaccgaga caggccctgc ggggctgcac
    13381 acgcgccccc acccttcggg tagggggaaa ggccgctaaa gcggctaaaa gcgctccagc
    13441 gtatttctgc ggggtttggt gtggggttta gcgggctttg cccgcctttc cccctgccgc
    13501 gcagcggtgg ggcggtgtgt agcctagcgc agcgaataga ccagctatcc ggcctctggc
    13561 cgggcatatt gggcaagggc agcagcgccc cacaagggcg ctgataaccg cgcctagtgg
    13621 attattctta gataatcatg gatggatttt tccaacaccc cgccagcccc cgcccctgct
    13681 gggtttgcag gtttgggggc gtgacagtta ttgcaggggt tcgtgacagt tattgcaggg
    13741 gggcgtgaca gttattgcag gggttcgtga cagttagtac gggagtgacg ggcactggct
    13801 ggcaatgtct agcaacggca ggcatttcgg ctgagggtaa aagaactttc cgctaagcga
    13861 tagactgtat gtaaacacag tattgcaagg acgcggaaca tgcctcatgt ggcggccctc
    13921 gcgagaatta attcagataa aaaaaatcct tagctttcgc taaggatgat ttctagcgat
    13981 gaccctgctg attggttcgc tgaccgtaga ctggttggct tggtttcatc agccatccgc
    14041 ttgccctcat ctgttacgcc ggcggtagcc ggccagcctc gcagagcagg attcccgttg
    14101 agcaccgcca ggtgcgaata agggacagtg aagaaggaac acccgctcgc gggtgggcct
    14161 acttcaccta tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc
    14221 ctttggcaaa atcctgtata tcgtgcgaca tcgcgcgcgc gcgcgcggat gctagcggcg
    14281 cgccaaaaaa aagcccgctc atta
//
