LOCUS       sequence-466011-       12654 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh1 and (+)-Eldh2 
            in Eggerthella lenta DSM 2243 with a cumate inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12654)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 12654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
COMMENT     Sequence Label: 
            pXD70EN19-Pct5-GPA_25810/25800-(+)-Eldh1/2(GpDSM19378)
FEATURES             Location/Qualifiers
     source          1..12654
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      24..95
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(276..864)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(356..375)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1035..1826,1827..1895))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1658..1677
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1896..2000)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2068..2086
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    2243..2262
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             complement(2265..2876)
                     /codon_start=1
                     /gene="cymR from Pseudomonas putida"
                     /product="cumate repressor (Mullick et al., 2006)"
                     /label=CymR
                     /translation="MSPKRRTQAERAMETQGKLIAAALGVLREKGYAGFRIADVPGAAG
                     VSRGAQSHHFPTKLELLLATFEWLYEQITERSRARLAKLKPEDDVIQQMLDDAAEFFLD
                     DDFSISLDLIVAADRDPALREGIQRTVERNRFVVEDMWLGVLVSRGLSRDDAEDILWLI
                     FNSVRGLAVRSLWQKDKERFERVRNSTLEIARERYAKFKR"
     protein_bind    3214..3241
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     terminator      8681..8708
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(8831..9625)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 agcttgctgg ggcttttctt tttcaaataa aacgaaaggc tcagtcgaaa gactgggcct
       61 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gaggagctca tgccggcggc
      121 acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg
      181 tgaatgcgca aaccaaccct tggcagaaca tatccatcgc gtccgccatc tccagcagcc
      241 gcacgcggcg catctcgggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
      721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      841 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      901 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
      961 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1021 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     1081 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     1141 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     1201 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     1261 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     1321 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt
     1381 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     1441 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     1501 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     1561 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     1621 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga
     1681 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     1741 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     1801 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     1861 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     1921 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     1981 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     2041 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa
     2101 ggcgaaaaaa ccccgccgaa gcggggtttt ttgcgaaata taatgaccct cttgataacc
     2161 caagagggca ttttttagga acggttattt gattattaat ccggcggcaa ctgaaacgat
     2221 tcggatcctg tattactatt ctgaattcgc ggccgcttct agatctagcg cttgaatttc
     2281 gcgtaccgct ctcgcgcaat ttcgagtgtc gagttcctga cacgctcaaa gcgttctttg
     2341 tccttctgcc ataggctacg aacagcaagc ccacgcaccg aattgaatat caaccaaagg
     2401 atatcttctg catcatcacg cgaaagacca cggctcacca gaacaccaag ccacatatcc
     2461 tcgacgacaa accgattcct ctctaccgtg cgctgaatac cctcgcgtaa cgctgggtcc
     2521 cggtcggcag ccacaatcaa atcaaggctg atagagaagt catcgtcgag gaaaaattcg
     2581 gcggcgtcgt ccagcatttg ctggatgacg tcatcctctg gcttcaattt cgctaatcga
     2641 gcccgactgc gttcggtgat ctgttcgtaa agccattcaa aagtggcaag cagaagctca
     2701 agctttgtcg ggaaatgatg gctctgcgct cctctggaga caccagcagc accgggcaca
     2761 tctgcgatcc ggaatcccgc gtaacctttt tcccgtaaaa cccccagggc cgctgcaatc
     2821 aacttgccct gggtctccat tgcgcgctct gcctgtgttc ttctctttgg actcatgatc
     2881 accatttttt ttactaaagc ttgatctgca atttgaataa taaccactcc tttgtttatc
     2941 caccgaacta agttggtgtt ttttgaagct tgaattagat atttaaaagt atcatatcta
     3001 atattataac taaattttct aaaaaaaaca ttgaaggaac ggttatttga ttattaatcc
     3061 ggctttttta ttatttaggc aactgaaacg attcggatcc tgtattacta ttctattttg
     3121 tcaaaataat tttattgaca acgtcttatt aacgttgata taatttaaat tttatttgac
     3181 aaaaatgggc tcgtgttgta caataaatgt agtaacaaac agacaatctg gtctgtttgt
     3241 attatgatta actaataagg aggacaaaca tgtcgaacga ttactacgaa agccaccgag
     3301 gcgaatggca gtggcaggac ggcgagtaca ccgtcacgcg cacgaccgcc tggtcggggc
     3361 ccggttgcca cgacggctgc ggcgtgctgt actacacgaa ggacggcaag ctggagaagg
     3421 tggagggcga ccccgacaac ccgttcaacc agggcgcgct gtgcatgcgc tgcctggaga
     3481 tgcccgagtt cgtgaacaac cccacgcgcc tcggccaccc catgaagcgt gccggcgagc
     3541 ggggcgagaa caaatgggag cgcgtgtcct gggaggaggc gctcgacctc atcgaggaaa
     3601 aggtacgcgc catctggaag gactacggcc ccgagtccat cgtctgtcat atcggcaccg
     3661 ggcgcaacaa ctgcgaccag ataccgtacc tgtgctacgc ggcgttcgga tcgccgaact
     3721 tctccatggg ctatctggcc ggcgactcct gcttctgccc gcgctccgcg tcgatggcgt
     3781 cgcagaacgg cgacttcatg atcgccgact gctcgcagca gttcgagaag cgctacgacg
     3841 agagcacggg ctggcgctgc cccgaagtca tcgtgaactg gggctgcaac gcggtcgtct
     3901 cgaactccga caactttttc ggcggctgga tcgtggactg catgcagcgc ggctccaaga
     3961 tgatcaccat cgacccgtcg ctcacctggc tggcctcccg ctcggcgtac tggctgcgcg
     4021 tgcggccggg caccgacgcg gctttggcca tggccatgct caacgtcatc atctccgagg
     4081 acctctacga ccacgatttc gtggccaagt ggtgctacgg cttcgacgag cttgccgagc
     4141 gcgtgcgcga ctggacgccc gagcgggctg cggaggtttg ctgggtcgag gccgaggaca
     4201 tcgtgggcgc cgcccgcctg tacgcccagg ccgaccccgc cgccatccag tgggggctca
     4261 agctcgacca gaccaccaac gccaccgcca ccgcgtgcgc cgtcaactcg ctgtgggcca
     4321 tcacgggcaa cgtggacaat cccggcggca acatcatcat ccgcaacgcc ttcgaccaga
     4381 acctctcgta cggctacggg taccacctgc tgccgcccga ggtgcaggcg aagcgcctgg
     4441 gggccgagtt cccgctgatg agccgtgcgg gctacagctc ctccgcccat gcggactccg
     4501 tgctgaaggc catcgagacc ggcgagccgt atcctatccg catggtgtgg atgacgtcca
     4561 cgaaccccat cgccaacatg ggcgccgacg cgccgcgcct gttccgcgcc ttgaagagcg
     4621 tcgatttcgt ggtggtgaac gacctgttca tgacgccaac cgccatcgcc ttcgccgatc
     4681 tggtgctgcc cgccgccatg tctcccgaac gcaactcgca gcgctgctgg tgggttccga
     4741 accgcacgct caccaaggtc acccagtacg aggagtgcgt ttcggacgac gacctggtca
     4801 ccatggtggg caaacgcctg caccccgaga acttcccctg ggacgacaac gtgggatgga
     4861 tcaaccacat cttcgagcac gagacgaacg acgagctgcc ctacaagagc ttcgaggagg
     4921 cgcagaaggt cgtgtggtcc tacccggcgt tcacctacct caagcacgag aagggcctgc
     4981 tgcgccccga cgggcagccc gggttcaaca cgccgtccgg tcgcatcgag ctgtacaaca
     5041 cgaacctcat gctgtggggc tacgatccgc tgcccgactt caaggagccc agctgctctc
     5101 cggtgagcag ccccgagctc gccgagaagt acccgctcgt cctgaccacc ggcgcgcggt
     5161 ccttcgagtt cttccattcc gagcatcgcc aggagggcac gacgtcgcgc gagctgcacc
     5221 ccgacccgct gttcgagatg agccctgccg ccgccgaggc cgcgggcctc tccgagggcg
     5281 attgggcctg gatcgagaac cagcgcggcc gctgccgcca gaagctgcac gtgaacccct
     5341 cgctcgacga ccgcgtggtg cgcgccgagc acggctggtg gttccccgag accgaggggg
     5401 cggagccgaa cctgtacggc gtgttcgact ccaacatcaa caacctcacc ccgcagtgcg
     5461 agaacggcga caccggcttc ggcgccccgt acgcgaacca gctgtgccgc gtgtacccgt
     5521 gcactgagga gaacagccgt gtcctgccgg gcgagcaggt caccaggcgc gggggctatt
     5581 ccaaggtaat cgacgagtcc aacggcgacg tgaaggtggg tgctatctat ggctaacgca
     5641 ttgatgatcg actacgagtt ctgcacgggc tgccacgcct gcgaggtggc ctgcaagaag
     5701 catctgggcc tgcccgcggg ccagcacggc atccgcgtgc tccaggacgg cccccgcgag
     5761 ctcgcgggcg gagggtggga gtacaacttc ctgcccatgc ccacgtcgct ctgcgacctg
     5821 tgcgcctccc gcctggccga gggcaaggac gccgcctgcg tccaccactg cccggcccac
     5881 tgcatggcgc tcgggccggc cgaggagctg gcggcacggt tcgacgggaa ggtcaaccgc
     5941 gcactgttcg ttcccgcgca gtaacgacgg ttcgcgccgg gcgccctgcc gcccggcgcg
     6001 gttatggaag gagaagcaat ggatcaggcc aatagctact acgaggccca taagggcgag
     6061 gtggagtggc gcgacggcga ctacacggtg acgcgcacga cggtgtggtc ggggcccggt
     6121 tgccacgacg gctgcggcgt gctgtactac acgaaggacg gcaagctgga gaaggtggag
     6181 ggcgaccccg actgcccctt caaccagggc acgctgtgca tgcgctgcct ggagatgccc
     6241 gaagccgtga accatcccga ccggccgacc ggtccgctca agcgggcggg cgagcgcggc
     6301 gagaacaagt gggagtccgt cacctgggac gaggcctacg acattatcga ggagaaggtg
     6361 cgcgccatct ggaaggacta cggcgccgag tccatcgcgt gcatgatcgg caccggtcgc
     6421 aacaactgct ggcagatccc gtacctgtgc tatgcggcgt tcggctcgcc gaacttctgc
     6481 ctgggattcc tctcgggcga gtcgtgcttc cagccacgct cggcggccat ggcctccatg
     6541 aacggcgact tcctcatcgc cgacatgtca cagcagttcg agagccgcta cgacaccgag
     6601 aacaccgagt gggttccgcc tgagatgatc gtggtctggg ggtgcaaccc catcgcctcg
     6661 aactccgaca acttctacgg aggctgggtc gtcgactgca tgcagcgcgg cagccggttg
     6721 atcaccgtcg acccgtcgct cacctggttg gcggcccgtt ccgactattg gctgcgcctg
     6781 cgccccggca ccgacggcgc gctcgccctg gccatgctca acgtcatcat ctccgaggac
     6841 ctctacgacc gcgagttcgt cgagaagtgg tgctacggct tcgacgagct tgccgagcgc
     6901 gtcaaggaat ggacgccgga gcgggcggag gcggtgtgct gggttccgaa ggacaccata
     6961 atcggggcgg cgcgcgccta cgcgaaggcc aagcccgcca ccatccactg gggcctgccc
     7021 attgaccagg cggttgtcgg catccccacg gcgcaggcca tcaacgcgct gtgggccgtc
     7081 accggcaaca cggacgtgcc gggcggcaac atcatcatcc gcaacgcgtt cgagcagaac
     7141 gtgtcgtaca actacggata ccagaccctt cccgccgagg tgcaggccaa gcgcatcggc
     7201 gcggagtacc cgctcatgtc caaggccggg ttcagctcca cggcccactc ggactccatc
     7261 ctgcaggcca tcgagaccgg caagccgtat cccatccgca tgctctggct ttcctccacg
     7321 aaccctatcg ccaacatggc gtccgacgcg ccgcgcgtct accgggcggt gcgcaccctc
     7381 gacttcgtcg tggtgtccga cccgttcctc acccccacgg ccgtggcgtt cgccgacgtg
     7441 ttcctgccgg cgtccatgag ctgcgagcgc aacacgcagc gcgtatggtg ggtgccgctg
     7501 cgcggcatga agaaggtgac gcagttcgcc gacgtgcgtt ccgacgacac catcgtcacg
     7561 gacctcggac gccgcctgca tcccgagaac ttcccctgga ccgatgacat cggctggacg
     7621 aacgatatcc tcgtcaacga cacgccaggt tacgacggcg acttcgagca gctgtgcaag
     7681 gacgtgtacg tctatcccgc gttcgagtac cgcaagcacg agaagggcct gctgcgcccc
     7741 gacgggcagc ccggtttcaa cacgccgtcc ggccgcatcg agctctacaa cagcatcttc
     7801 gcgctgtggg gctacgaccc gctgccgcag tgggaggagc catcctgctc gccgtacgcc
     7861 tcgccggagc tgtacgagca gtaccccttc gtgctgacga cgggcgcgcg ctcggtggag
     7921 ttcttccatt ccgagcatcg ccagctggag acgagccgcg agttccaccc cgatccgctg
     7981 ttcgagatga gcccgaaggc cgccgaggcc gcagggctgt ccgaggggga ttggtgctgg
     8041 ctggagaacc atcgcggccg ctgccgccag aagctgcacg tgaacccctc gctcgacgac
     8101 cgcgtggtgc gcgccgagca cggctggtgg ttcccggagc aggaggccgc ggaacctgtc
     8161 ctgttcggag tgttcgactc caacatcaac aacctgctgc cgcagtgcga gaacgggccg
     8221 accggttacg gtgccccgta caagaaccag ctgtgccgtg tgtacgcctg cacggaggag
     8281 aacagcgccg agctgcccac ggcatgcgtg acgaggaagg acgggtactc gaaatgagcg
     8341 ctaacgcatt gatgatcgac tacgggttct gcacgggctg ccacgcctgc gaggtggcct
     8401 gcaagaagca tctgggcctg cccgcgggcc agcacggcat ccgcgtgctc caggacggcc
     8461 cccgcgagct cgcgggcgga gggtgggagt acaacttcct gcccatgccc acgtcgctct
     8521 gcgacctgtg cgcctcccgc ctggccgagg gcaaggacgc cgcctgcgtc caccactgcc
     8581 cggcccactg catggcgctc gggccggccg aggagctggc ggcgagggct gccgagaaga
     8641 cgcggtgcgt ggtcttctcc tagactagtc gactcacact ggctcacctt cgggtgggcc
     8701 tttctgcgtt tatatactag agagagaata taaaaagcca gattattaat ccggcttttt
     8761 tattatttaa ttcctgttat aaaaaaagga tcaattttga actctctccc aaagttgatc
     8821 ccttaacgat ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag
     8881 gcttgatccc cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc
     8941 tgatcgaccg gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa
     9001 gatcaataaa gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc
     9061 cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc
     9121 gcggatcttt aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt
     9181 tattcagtaa gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat
     9241 ccgatatgtc gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt
     9301 cagggctttg ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga
     9361 gcagattgct ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc
     9421 cttccagcca tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc
     9481 ggctgtccgt catttttaaa tataggtttt cattttctcc caccagctta tataccttag
     9541 caggagacat tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt
     9601 ccggtgatat tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa
     9661 gataccccaa gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta
     9721 aaaccttaaa taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat
     9781 agtatcgacg gagccgattt tgaaaccaca attatgatag aatttacaag ctataaggtt
     9841 attgtcctgg gtttcaagca ttagtccatg caagttttta tgctttgccc attctataga
     9901 tatattgata agaagactgg aatgcccgct ccgccgggat tattcgaaag cgcatcgtcc
     9961 aagcgagacc gtctgcattc ggaaatctcg gccggttcgc gccgaggaag cccatctggc
    10021 tgcatcccaa atcgacacac aacgcacgga ggccccggcg atcgccgggg cctccgaatc
    10081 atgcgcatcg tggaagcgaa gctccgcaaa aaaccccgct tcagcggggt tttttcgcgt
    10141 cgcacctcct tgtcgtgtat tacaggtact actataacac atcatctagc ccgcccgcga
    10201 tacgtcctct gcggtgcagc gtggtcgcgg ttccacgcat ccgccgcccg ctgcgcatcg
    10261 cgcagcacgt cattaaggct ctgttgcctt ggctgttccc tgcgcatcag atattcgagc
    10321 gggcgcgata ttcgacttct aagctcttcc actttgattc gcattcccgc aactcctgct
    10381 ctgatgcggc tcgcgagtcc atcgcactta acaacgatgt tatcgaggat tgcgccgcgc
    10441 tcagcttttc ccgcagcttc ccagccccga acctcggtag cgatgggttg aagctctcca
    10501 atgtctcgcg caactccgtc cgctcgttgc cgaagacact ccaatcggtc ttgctcatct
    10561 tcgatatcga gcttcacgtc ctcgagctgg ctgtcaagct cggcaaggtc accctccttg
    10621 tcggcaatct cggtaacaag ctctgcctta cgcgtttcgg catcattcag taaagcggcg
    10681 gcttcttccc tagccgccgc gacgattcgc gccgcttcgt ccttcgcagg ttcaaccacg
    10741 gccttatcga ccgcacagcg taccttgtcg atatccacgg acttgtccgt gtatacacgc
    10801 tgcgcccgcg tctcctcgtc aagctcgatt gcagggcggt agcccatgcg cccctctagg
    10861 taatccccaa gctccttgtg catgctccgg tagaagctgc gggggcacat ctgcttgtag
    10921 ttgaaccgtc catctaggat gggcgtgaac ggcacgtgca tgtggtcgcg ggcgggcgtg
    10981 ccatccttca acacctcgtc cttgtggacg tacccgccca tcatgttctc gatgccgaac
    11041 ttcttcgaca ggtaccagta cgtgagacgg aagaacctgt cctcatcgcc cttgcgcaca
    11101 ttgtcgggaa gcgtcacaac aacgtcagcc ataacgaccg catccttgcg cgtggcacgc
    11161 ttccctgcgg ccttctgggc ctcgttcacg cgctcgatgc ggctctccac cgtgccccag
    11221 ttcggctcgc catcgcgcgg ctccacgcgc cccacaacga gcttgccgtc cttgttcgtg
    11281 tagtggccga ccaccaagtc gttcttgatg cgctcggggt cgatgttgtc gcgctccagg
    11341 ctgctgcggt cgcggcggta gtgggcaagc atgtgcccga ccgatggagc cttgtatttc
    11401 gcgatgtgag ccatggaaac ctccgaaccg tacagactta atcgactaaa gtctagcacg
    11461 ttagacctta gctacgctaa ggacgtggca gggccagccc tgcacccgct cgggcgttcc
    11521 ccccgagacc ccccagccct tgcccgttcg cggcttcgct cacggccctc gggccatcgg
    11581 cgcgagcgcc gaacggaggg acttcgcccc tccgaaccac ccctcaatct acctgcggtt
    11641 cggcacacgg cacacggcac tattaggggg gccgtcgtag tggcccccct gtggtcattt
    11701 gtcatttgtc atttgtcaca ttatttttcg gacacattat ttttcggacg tagttagcga
    11761 aaaataatga gccgcccgat tctgcaggtc tcatccagtc gagacacggg caaggaaagg
    11821 gtcgatggag cagagcgtcc atcgaccctt aatcaagtct ggtctattcg gttggccgtc
    11881 gcgtcaattc cgcaatccgt gaacgagtcg gcttagatct gcgcgagcgc agaccacgcc
    11941 gccccgtagt cacgcattgc tgcattgacc cgactactct ttggcacctg actgttccac
    12001 gcacttgcgc ttacgttcgg cgttcagata gttcccgcga caatagcgct cgagaatcga
    12061 gttgtacccc acgatgattt cgcggtactg ctcgggaaac tccctcagcg ccgcagccgt
    12121 gaaatcgttg atggtctcga acctctcgca catgatgaag ccgaccaact cgaagagcat
    12181 gtcctgcttg tccttagtcg agagctgttc gagctgacca atgtcgaagt tgtttccctc
    12241 gatgcgtgct tcgaccggat acagctcctt gcccttcttg cgacaggaag ccgtgtcgtg
    12301 aatcagatag tcatacgcat gcctgatgtt gttgattggc tcggctgacg aacagcactt
    12361 cttgccgtcc gcgctcaatc ggttcaagac gttgatgatt gccttgcgtg tcgtgttgcc
    12421 gccccagaca acaatgacgt gcgcatgcgt cttgcgctgc ttgctcttct ggtcatggtc
    12481 tatgtcgtga accgcataag cgaaaggcac ttgaagaatg tcgtctatct cgtcctgcca
    12541 gtcatcgcgc atgttctctg ggtagcagat gccctcccag agcttcacct tcgtctcgcc
    12601 agcgtttgcc gccatgctat aatctccttg tctcgaatgc ccctgctgcc gcca
//
