LOCUS       sequence-466013-       20501 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Eggerthella lenta A2 Dadh (Delta-Elen_0476) in 
            Gordonibacter urolithinfaciens DSM 27213.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20501)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 20501)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
COMMENT     Sequence Label: pXD80ENSF7.6-Dadh-Delta-Elen0476(ElA2)
FEATURES             Location/Qualifiers
     source          1..20501
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..813)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     1300..1318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1475..1494
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             complement(3005..3028)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     terminator      14125..14152
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      14284..14311
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(14743..15594)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(15581..16420)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(16931..17902)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            19141..19228
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      19570..19964
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(20127..20170)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     oriT            20266..20375
                     /note="incP origin of transfer"
ORIGIN
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagatttag
     1501 tacatcagga ccactgggta gcccagctgg tagatcagca cgcgaaacat cacggaggtg
     1561 gccagcgcgc cgatggcggc gacgaccatg agcgtcttgg aggcgcccac cttcttcttc
     1621 gccacgaagg cgcaggccgc agccacgatc acggagacga tggagcccca gaacatgggc
     1681 gctcccgcgc cggacaccat gacggtcatc aggctgtcga cgtgcgtggg ggccgtggtc
     1741 atcgatgtcg ggtcggcgta gtagccgaat tgcgcgatct tcgccatggc gcaggcgatg
     1801 acgaacacca cgtcggccac gatctgcacg agggccgcga tgaacgtcag ctggatgccc
     1861 gtcgcctcga cggcctcgtc cttcttcagg atggagatca gccagacggc cgcggcgccc
     1921 aggaggcacg cgttgcccag atagaacgcc acgagcgcca ggccccacgc gggacgggcc
     1981 ggcatgaggt aggagtgcgc ggtggcgacc atcatgagca cggcaaccgc gagggtcgcc
     2041 caggccagcg ccttgggaac gggcttgccg cctcgaagga ccacgaacca tgcgacgatg
     2101 acgatggcca gcgccacgca tccgatgagc tcctgggtga tgcccgacgt gatgtgcccg
     2161 aagccgttga agatgcgctc ccagtgctcg aggtgcagga aggctccgat gccgcctacc
     2221 accagcgaga cggcggacgt gatgagcgcg gtcatctgca ggttcctgcc ctggcccttg
     2281 agcgccagga tggacatgcc gcccagcgtg ccgcaggtca agcatacgaa cagcgtgaag
     2341 atgacgagag gccactgcgt ttccattact catcacctcc ttcccagtga tggtcgcgta
     2401 ggatgtagga gtacgacggc ttgttgccca cgtcgggcag cttgtgcacg tcggcgtcgg
     2461 tgaacggctc caggaactcc accatcttgc ggcgcttgcc cagcgggtcc tcggagccct
     2521 cgaacgactc gtagccggcc tcggtgtcgc cgatccagcg ggcgttgccg ccgcactggc
     2581 tgacgcactg gggcagctcg ccctgcgaca gcttctgctc gcacagcgtg cacttctcga
     2641 cgaccttctc ctcctcgttg aggtagcgga cgccgtaggg gcaggccatg acgcagaact
     2701 ggcagccgat gcacttctcc ttgtcgatct ggatggagcc gtcctcggcc acgtgcgacg
     2761 cctcggtggg gcacaccttc acgcactcgg ggttctcgca gtgctggcag ctgatgggca
     2821 ggaagtacat gtacacgtcg gggtactgcc ctgatccgcc cgggatgggg ttcggcccca
     2881 cgcgcaccac tttgttccag tagctgccga tgggcacgtt gttgaccgcc ttgcaggcga
     2941 ccgagcacgc caggcagccc acgcaccggt tgaggtcggt gatgatagcc ttcgtcatgg
     3001 ttcagtgatg gtgatggtga tggtgatgct cgagtgcggc ctccctccct tcgtaggtag
     3061 gcagccattc cttcagacgg gggtcgtcgg aggtatggat gatgggcgtg ccggtggagt
     3121 cgcacggcac ggggttgttg aacggcgagt tctcgggcgt ggccttgtag atcttcacct
     3181 ggtaggcgcg caggttggac gtgccggagt gcgggtcctg ggcgtagtgg tcgatcagct
     3241 ggttcacctg ggagaactgc cagccgtggc cggcgtcctt cacctcgggg taccaccacg
     3301 tgtgctcgag gttgatgacg tcttccttca cgccgtagta caggtcagcc acttcgcgga
     3361 tcttgcccca ctcggtttcg atccacaccc agtcgccctg ctcgatgccg tactcggcgg
     3421 ccgtcttcgg gttgatctcc acgcgcggca cgggccacag ctcgcggcac cagggaagct
     3481 ggcgatgctc ggagtggaag tacaccggga tgcgacggcc ggtggtggcg gtcagcgggt
     3541 actccttgat gcggtcgccg tccttcgggc cgtgaggcgg ctcggtgtag gtgggaagcc
     3601 tccactcggg atggtcggga tggtgcgatt ccatgacggt ggaccagatc tcctgcttca
     3661 tcgtcggggt gaaccagccc ggcttccagt cgccgatgcc cttgccggcc gtgtagtcga
     3721 ggcggcccca cacgcggtcg cgtgcgcgca tcgcgccggt ctggtagcgg cggtacgtgc
     3781 cccagttgcg cggctcgatg tccttcgcct gccaccagcc gtgctcctgg aaggccttga
     3841 cgtactcgtc ccacgtcttg tacttgggcg tgacctcggc catcggaacg ccgtggcgct
     3901 cgaacgtggg cttgccgttc tcgacgtgcc acgtggtgta ggtcagctcg tcgtcggtga
     3961 gcagcttgat ggagtcgtcc agctgccagt tgatgtcggg ccactcgttg cccggctcgt
     4021 cggtccactt ccagttcatg cggcgggcga ggtccatgac gatctcggga tcgtacttgg
     4081 cttccgcggg cggctgcacg cacttgaccg tggcgcccat ggcgccggcg gaaccttggc
     4141 tggcgcgcgg cgagttgagc tcgatccaat gggccaccgg caggatgatg tcggcggtgt
     4201 cgttctgcgg cgtgtgccac aggttgaggt ccacgtagaa gtcaagcgag cacagggcct
     4261 cccaggcgcg cgtggtgttc gtctggctca tgaagttgcc ggactcgttc cagagggcgc
     4321 gcaccgggta ggggtcgccc gtgatgacgg cgtcccacag cgaatggctg tcgcaccagt
     4381 actgccacca gcccagaagc gggaacttgt cgatgccgat ctgcttgcgg ttgacttcct
     4441 ccggcggggt ggtggcgccg ggggcccagg cgctgaagcc ctggaggtcg ccgtcgatgg
     4501 ggacgatggt cgggccgcgg ttgccgcccg gggtgtccat gttgccggtg atgcccacga
     4561 ggttgtcgaa ggcgcggcag ttctggatgg cgctgcagaa gtgctcgacc gccagcatgt
     4621 actggatgcc gccgttgccg tagccggtgg acgggtcgat gcgcgtgccg taggccgtgg
     4681 ccgccgcctc gatctcggag gcggggatgc cggtgatctc ggccgccacc tcgggcttgt
     4741 actcggcggc gcgggccttg tagtgctccc acaccggctt gaccttcacg gtcttgccgt
     4801 ccttcagcgt gatctcgaac tcgccttcca gcgcggggtc gatggcgggg tcgaacggca
     4861 tgggatcggg cagccagccc tgcgtctggc ccggcagcag gttgtcctgc gcggcctcgc
     4921 ggccttggcg cgccttctcg gagtcgtagt tctcgccctc ccagaagccg ccggtctcgt
     4981 cgatgacgcc ttcctggtcg gcgttgaacc aggtgaacgc gccgtactcg tgctcgacgc
     5041 cctcggcctt cagcttctcc cagttgttgt cgtacacgag gaacttatag gggctgccgc
     5101 cctccttgat gtcgctttcc ttgagcagcg cggttttcac gtcccagtag gaaccgtcgg
     5161 tgcgcacggt ggggaagccg gagggctcca tgtcctcgca cacgaggaac ggggcgttcg
     5221 tccacttctt cacgtacagc tcgtcgtaga gcttcttctc gatgatgacg ttcgtccagg
     5281 ccagggccag ggcgccgtcg gtgccggggc gcaggtgctg ccagtagtcg gcttccttgc
     5341 ccatgttggc catgcggggg tcgacgctga tgtgcacgtc ggcgcgcgat gccacgtcca
     5401 ccgtggtgcg gcaggagtcg tcgtagttgg acagctcgga cgcgccgccc cactgcacgt
     5461 acactttcgg gcgcgtgatg gtttccatcc acgacatggc gaactgcgac accatggtgg
     5521 tggcgaagtg gcgcgggccc ttgcagatct gccaggcctc aacgttgttc ggcgtttcca
     5581 gcatgttctt caggatggac tccgagtgca tgcaccagat gcgcgacgtg cccacctggc
     5641 aggcgatggc ctcgccgccg tgcttcgcct tgatgtccat gaagttgtcc acgatggact
     5701 gcatggcctc gtcccacgag atgcgctgcc agccgggctc ctcgcccttc gggttcgtgc
     5761 gcttcatggg atagtgcaga cggtcggggt ggtacgccgc ctggatggac gactgcgact
     5821 tcgtgcagca gttgcccatg gactggaacg ctccctcgtc gccctcgacg cgtatggccc
     5881 gcccgttctg gacgatgacc ttgacgccgc actccatctt gccgcagccg cggcagcacg
     5941 ttttcacggc cacggtgtcg ttgccggaca cgctgctgta cgtctcttcg gccagcgcgg
     6001 tgtgcgtcga agcgccgaac gccgcggctg ccgcaccggt gatggcggcg gtcttgacga
     6061 acgtgcgtcg tgacatggtc aggttaccca tactcctcct ccttctttct cctcgttctc
     6121 gtcttgctcg tcttcgttcg tgcgatcggg gaggggggct gccgggtgaa gcctcgggct
     6181 ccgaaccggt gcaaagtccc cgtgtcgctt gggccgacca tacggatgcg agggggtgca
     6241 gagcatcgtc caaaggttgg aaagcaggtt cgaccgcatc ataaattctt atgatcttgt
     6301 gatcatgaaa aattcgatag tgctttgaac tggggaaact ttaaccgtag gtcataaatt
     6361 attctgaaac tagaatagtt tcctcaaaga gggccgctca tcttttccgt ataatcgaac
     6421 ccgtcgacag aggggtcggc gcgagcgacg ttatgtacgg gctcacaggg ggtggggcat
     6481 ggaaaagctt cggagtgcat gggataccgc gttggggacg agcctttccc tcaagcgcat
     6541 cgcgggcgcg gccttgcaat ggctttggat gctcatgatg ttctacagca tcgtgccgta
     6601 cggcttctcg tcggacgtgc gcggctcgct gtacgtcagc ctgatcatat cgctggtggc
     6661 catggtggtg accatgctgt tcgtctccgt catcgtgcgg cgcgagcggg tgagcggcaa
     6721 ccgctccatc gtgctgggat cggccatcat gatgagcgcg ggcagcgtgc tcacgccgtt
     6781 ctccgatcct accacgcccg tcggcatgct ggtgctgggc ctgagcgcgg tcatgaccgg
     6841 cacgggctcg gccgtgctgt tcttgtgctg gatcgagctc gagtcggggc tgggaggccg
     6901 cttggcgctt gtggagcttg cctcgtcgct gtgcatcgcg ttcgtcgtgg ggttcttgct
     6961 gatcgtaggg ccggcgatac ccgtcatcgt gctcatcgtt gtcatgcccg tgctttcggc
     7021 ggcagctctc aggcgcagcg cgcccgatac gccgcagctg ccgcgcgagc ccgagcagcc
     7081 tttgtcgcgc catacggcga ccctgttcgc gaaggcgctt ctgggcgcgg tgctcatcgg
     7141 catgttgatg gggttcttcg acgtggtgtc ggggttcaaa acctacgagg tgcaggacat
     7201 cttcggcacg tacctgtttc tggcgggctt catcggagcg ttggccatct gcctgatagc
     7261 cgtgttcctg cagcgcgaca gcgtgttctt ctcgtaccgc gtctccatgc tgatgctgtg
     7321 cttgggatgc ttgtccacgc cgttcatggc cgacaacaac acgtattcga gcgcgcttat
     7381 cttcggcggc taccactgct tcgtgctggt gctgtgcgtg gtgtgcatcg acgtggcgtc
     7441 cagcttccgc gtgagcccgg cgcggacgat cggcctgggg ttcgtggcgc tgtacggcgg
     7501 cgagaccgca ggccagctgt tcgcccattc gctggaggcg accggggtca gcatgttcga
     7561 cctcacgctg gtcacgctgg tggccgtgtc gctgctgttc atcgcgcatt tgttcctgtt
     7621 cacggagacc gacctcatca aaatcggcat cggcgaggtc agcctgatgg gcacggtgcc
     7681 cgaagaggcc gccgacgacc cggccgaccc ggtggatccg tgcgcgctga tcgcggagcg
     7741 cttcgccctg tcgccgcgcg agaccgacgt gcttccgctg ctgctggagg gccgcaccat
     7801 ctcgcgcatc caggagacgc tgttcatctc ggcgggcacg gtgagcacgc atatccgcca
     7861 catctaccag aagacgggcg tggacaatcg ccaggagctc atcgacctgg cccacgagac
     7921 ggccgcgcag gacgaggcgg tctgaggcga tggagacggc gtatctgcac gagttcgcga
     7981 aggtggccga ttgcggcagc ttcacggccg ccgcgcgcga gctgcatctg acgcagtcga
     8041 cgctgagcaa gcacgtggcc ttgttggagc gcgaattcgg ggccgacctg ttcgtgcgcg
     8101 accgcagcgg cgtgagcctg accgaggcgg gcggcgtgct gtacgcccag gcgcggcaga
     8161 tggagaagct gctgcgcgcc accgagctgc tggtgcgcgc ggcgcgcgac gggcagggtt
     8221 cggcgggcgc gggggttgcg ggcgcgacgg cgggcgcagc ggcgggcggg ggtgccgtcg
     8281 acggctcgcc tgcggccgcg tgcgacacgg cgcttcgctg caagtgccgg ctcgcggccg
     8341 agcgctgcgg gctcgaccgg cgcgagctgg gcgcgctcat cctgtacgtg gaggagcgcg
     8401 gcttcgaggc gatccagggc gagctggcgc tgacgcgcga cgaggtggcc gacgtgctgg
     8461 gcagcgtgta ccgcaagctg ggcgtgggcg acaagcagga ggcgctcgat ttcatccatt
     8521 ccgtttcgga atgatggtag acgcgcccgc ggcggttggg catactgcgt agaagtgaaa
     8581 gcgctatgaa gctggggggc ttcgagcaaa gggcttcatc cgaagaatgg atggagaccg
     8641 aatatgagtg cttggactgc tgcgtccgtc gaagacgcgg cgaacaatct tcttgaagaa
     8701 ctgaatagcg gcgcgatgcg cttgtgcgtg gagcatgacg cgtccgacga cgttgcgccg
     8761 gcgttgctgc gcgcggctcg cgctcgcgtc gaggcggggt cgaacgtgcg cgtcgtgtgc
     8821 gccgacgccg ttgcagctgc gcgctttcgg gcgcttgcgg ccgacgatcc gctgctgggc
     8881 gcgctcgacg tggtatcggt gcgcgagctg gccctctcga ttctaggcga cgcgcgggtc
     8941 ggcgatgccg tgggacgcga cgcgcgcgtg ctggacgaga acgagctggc cgtgctcatg
     9001 gaggacgtga aggtgagcgg cctcaagccg ggccggctgc gcgagatgct caagttcttc
     9061 tacaagagca tcagcgactg cgccgacgat gacgagcgct ggctgatcac cgccgaagag
     9121 cagaccgtgc acgccatcct caccgagaac ctggaggcgc gccgcgcgtt gctgccgtgc
     9181 gaggtttcgt cgctggcgta tcgcggcctt gtgaaagccg gggtcgagcg cgagccgctg
     9241 acgctggtgg cagacgactt cgggtcgctc agcaaggcgt cgcagcgcct cgtgcgccat
     9301 ctggcgacgg acggcctcgt ggcggcgggc cgcacgctgg cctcgtccag ctccgaggag
     9361 ccgtatccgt gcttcgacgg gttccgcgcg cttgccgacg aggccgactg cctggtgacg
     9421 ctggcgtgcg agcggccggc cgcggcgcgc gagtcccgcg cgctggacga cccggcggcc
     9481 gagttcgcgt tcgcggcggc gtccgtggag gagtgcctgg ccgacggctt ccggccaggc
     9541 gacgtggctg tggccgtgcc gaacgccgct tggggcgagc gcatcgccgc cgagctggaa
     9601 gggcgcggca tcgccgtgga gcgcgggttc gacagcggga agatcaaggg cgacccgcgc
     9661 accgagggcc gttacgccga cctcaagctg gccgcgttcc tgcggttgta cctcgatccg
     9721 cacgatttca cgtcgttgcg ctcgtggctg gggttcggcg attggctgct gcgctccgat
     9781 gcgtttttgg agctgatggc gtacgcgcgc gaccatgaga cgaccgtgcc cgaagccgtg
     9841 gcgcagctgc gcacgcagcg cgacgccgat cgctcctcga cgatattcgg gaagttcgac
     9901 ggcccgctcg acgagctgga cgagctgctg cgcgcctgcg aggaagggct gacgcgcgag
     9961 gccgccgtag cgctgttcga agcgcacgga atgccgttgt ccccgcgcca cgtggagctg
    10021 ctgggcgacg accctgcgca tgcggacgtc gagcgcttgg cgcgctgcgg gttcgcgaag
    10081 ccggtggagg atgtcgcgcg cgatgccgtg catgtggtct cctacgcgcg ctgccacggg
    10141 caccatgtgc gcacggtgtt cgtcaccggc ctggtgaacg gcttcttgcc ggcgagcgac
    10201 gccgtggacg acaagttcac catcgaccat cggcgcgtgg cgctcgaacg cgagcgcgcg
    10261 ctgttcctgg acgtgctgtc ctgtgcgagc gaacgagcgg tatgcacgcg gttcgtgcgc
    10321 gaccttttag agaacaccgc gttcacgaag gtgcagacct cgcgcgtgtt cgtgcgcgac
    10381 ggcgagcgct tcgcgcgcgt gacgccgtcg gagttcgcgt cgctgaccga cgaggtgctc
    10441 tctcccgcgc ccgacgcgcc tcgcgagctg ccgacgaagg tgctgtacgc gtcttcgact
    10501 ttgtagctcc aacggtctga cgaccgcgcg aaccttcgct gacttttcct gaccggtgat
    10561 tttgaaatga tgaaaggact gatagatatg tcccacgatc aggaaaccac catcgccgac
    10621 gtgctgcgcg gccgcatggc aacctaccag ttcctctcgc gcctgttccg cgtggaggtg
    10681 gaccaggagc tgtacgacac gctgatgtcc atgcgcttcc cggcgaacac cggcaacgcg
    10741 ctggtggacg aaggctaccg gatgatctgc ggttatctga gccaagccga cggcacggtg
    10801 ctcaccgagc tggcggtgga ctacgtgcgc gcgttcatcg ggtcgggcaa cgacggcttc
    10861 tcggccgcgt acccgtacga gtcggtgtac acgagcccga agcgcctcat gatgcaggac
    10921 gcccgcgacg aggtgctggt gctgtaccgc gccttcggtc tggacaagca ggaaagctgg
    10981 aaggagggcg aggaccatat cgcgctggag ctggagttcg agcagatcct atgcgagcgc
    11041 gctatcaggg cgtacgaggc cggcgacgag gacgagtgcc tgaagctgct gctgtcgcag
    11101 cgcaacttcc tggaggacca cctgctggcg tggtacccca tgatggcggc cgacttgcag
    11161 aagttcccgc agaccgactt ctacaagggc ctgggcaagc tgaccgacgg cttcctgcgc
    11221 aacgaccgcg aatttttgga cgccgtgctg tctgagaacg aagcggattg ccatccagag
    11281 cggaggccgc aggccgaagc cgaaggatcc cgtgcggcgt cggccgaaac cgaagtcgag
    11341 gttgcatgat ggacgagcac ggcatcgagc ccaagcgcga tacgggcccc tgcgtgtcgc
    11401 gccgcacgtt cgccctggga gccgtgggag cgtgcgccgt catcgggctg ggcggcgtga
    11461 agtacctgcc cagtgccacg ttgctgcgcc cgccgggcgg gcaggacgag gagcagctgg
    11521 cgcgcggctg cctgcattgc gagaagtgcc gcgaggtgtg cccgaagcat gccatcgctc
    11581 ctgctcatct tgaggacggc atcttgaacg cgcgcacacc gcgcatggac ttcaaaagcg
    11641 gctggtgcga tttctgcgag aacgagccgg gcgggccgaa gtgcgtcgcg gcgtgcccga
    11701 cgcatgcgct ttcgtgcccc gacccgtcgc aggcgatcat cggcaaggcg gagatcaacc
    11761 gcgactggtg ccttgccgcg aagggcatgg gctgccacga gtgcgtcgac gtgtgcaact
    11821 acgaggcgct cgagctgggc gccgacaacg tgccggtggt ggacgtggat gcgtgcaacg
    11881 gctgcggcgc ctgcgagctg gcgtgcatct cgctgtcggc aggttccatc acggccggcg
    11941 ccaccgatcg cgccatcgtc gtccgtccga ccgaagaagt ggaggcgtga tcaggatgaa
    12001 gaagctcaaa acctatcggc tgcggttcct gatcatgctg gcgctgctgg ccgtggtggc
    12061 cgtggggtac ttcacggccg gcggcatcgg caacttctgc ggcatcggct tcgagtccat
    12121 cacgctgctg tgccccctag gcgcgctgct ggccatgatc gccgagcgca cagccatccc
    12181 gatggccgtg atcagcgttg cggtcgtgct gctggtgtgt atcgtgctag gcaaggtgtt
    12241 ctgcgcctgg gcgtgccccg tgcacttcct gtcgcgagga aggaagaagg gggataaggg
    12301 ggacaaggag ggtaaagggg gcaaggttgc ttccccttgc tccgcgtgcg cttccccttg
    12361 cggcaaagcc aagggcatca agatcgacag ccgtcacggc atcctcgctg cggcgttggg
    12421 ctccacgctc gtcttcggct tcccggtgtt ctgcctcgtc tgcccgatcg gcctcacgtt
    12481 cgccacggtg ctgctggtca tgcgcctgtt cgcgttcggc gacgccacgt ggacggtcgt
    12541 cgccttcccg gccatcatcg cgctggagat cctgctgctg ccgaagtggt gccagcgctt
    12601 ctgcccgttg ggcgcgttgc tttcgctgtt cagcgggctg aaccggacgt tccgccccca
    12661 ggtggacgcc gagaagtgcc tgcgcgaggg tcgcggcaag gcctgcaacc tgtgcgagca
    12721 ggcatgcccc gagggcatca acctgcacga tgtcgcggcg ggcgagacca cgctcaacga
    12781 ctgcagcaag tgccgcgcgt gcgccgacgt gtgccccgag cgcgccatca cgttcccgtt
    12841 gctgccgaag aagcaggcgc aggcgacgcc gctgcccgtc gacgacgaga tcccggaaag
    12901 gggataggcc atgtccctga tcaacggcct gctggccctg ttgggcgaga tcgagagcga
    12961 tgccatcgcc gtgcatgagg aacgctgcat ctcggttcgc aaccgcaacg ccgactgcct
    13021 gcgctgcgtg gaggcatgca cgtcgggcgc gctggcgtac cgcgcgggcg agctgctcgt
    13081 agagcccgag cgctgcatcg gatgcggcac gtgcgccacg gcgtgcccca cctgcgccat
    13141 cgagctgcgc aacccgacgg atgccgagct gacggcgcag ctcaagcact ccatcgtggc
    13201 gacgaaaggc catccggtca tcgtgtgcga gcaggcgctc gaggcggcca acgtcgcggc
    13261 cgacgatgcg tcggccgcgt gcgcggtgcc gtgcctgggg cgcatggacg agtcggcgct
    13321 cgtggggttg gcggcgtacc gcgccttcga cgcgacgctg gcctgcggtt cgtgcgagac
    13381 gtgcccacat gctccgggcg gcgcgctcgt gcgcgaggtg gtggagggcg cgcgcaacct
    13441 gcttgaggcg ttcggatcct ccatgcccat cgacctggag gaatgcgtgc ccgagcgcgt
    13501 gttggagccc ggcgggtcgc acggccaggc aggcagcgac ggggtggggc ggcgcgactt
    13561 cttccgctcg gccaaggacg catcgacccg cgcagctggc gcggcggtgg ccgaggagtt
    13621 ggggatggtc gaggccgagc ccgttcctgc ggcgcatcgc aaagtgggcg cagatgggac
    13681 gctgtcgcag ttcgtcccca cgcgccgcgt gcggctgtac aactacctgc agcacgtggg
    13741 gcaaccgacg gccgacgagg tggagacgcg tgtcatcggc gccgtgtcca tcgatgccca
    13801 gaagtgcagt tcgtgccgca tgtgcgcggt gttctgtccc acgggcgcca tcaagaagct
    13861 cgacgagggc ggcgtgttcg gcatcgtgca ccgtccgagc gcctgcatgc agtgccgcct
    13921 gtgcgagcac atctgtcccg agcaggccat cgcggtgagc gggcgcgtgc ccatcaagca
    13981 gttcatgggc aaggaagccg tgtgcttcgc tatgaagcgt ccaacctgga ccccgaacaa
    14041 gccctcatcg atgtacgata agctgcacgc gacgctcggc gaacacttgg agatgtgcat
    14101 gttctagact agtcgactca cactggctca ccttcgggtg ggcctttctg cgtttatata
    14161 ctagagagag aatataaaaa gccagattat taatccggct tttttattat ttaattcctg
    14221 ttataaaaaa aggatcaatt ttgaactctc tcccaaagtt gatcccttaa cgatccctgc
    14281 aggagaaggc catcctgacg gatggccttt ttgaagcttt ctagaatcgg gggcccccgg
    14341 ggggattcta gaacaatgga agtcctcgtc tcgccctcga attagcccgc ttaatgagcg
    14401 ggcttttttt gaattaattc tcgcgagctg gcacgacagg tttcccgact ggaaagcggg
    14461 cagtgagcgc aacgcaatta atgtaagtta gcgcgaattg caagctggcc gacgcgctgg
    14521 gctacgtctt gctggcgttc gggagcagaa gagcatacat ctggaagcaa agccaggaaa
    14581 gcggcctatg gagctgtgcg gcagcgctca gtaggcaatt tttcaaaata ttgttaagcc
    14641 ttttctgagc atggtatttt tcatggtatt accaattagc aggaaaataa gccattgaat
    14701 ataaaagata aaaatgtctt gtttacaata gagtgggggg ggtcagcctg ccgccttggg
    14761 ccgggtgatg tcgtacttgc ccgccgcgaa ctcggttacc gtccagccca gcgcgaccag
    14821 ctccggcaac gcctcgcgca cccgctggcg gcgcttgcgc atggtcgaac cactggcctc
    14881 tgacggccag acatagccgc acaaggtatc tatggaagcc ttgccggttt tgccggggtc
    14941 gatccagcca cacagccgct ggtgcagcag gcgggcggtt tcgctgtcca gcgcccgcac
    15001 ctcgtccatg ctgatgcgca catgctggcc gccacccatg acggcctgcg cgatcaaggg
    15061 gttcagggcc acgtacaggc gcccgtccgc ctcgtcgctg gcgtactccg acagcagccg
    15121 aaacccctgc cgcttgcggc cattctgggc gatgatggat accttccaaa ggcgctcgat
    15181 gcagtcctgt atgtgcttga gcgccccacc actatcgacc tctgccccga tttcctttgc
    15241 cagcgcccga tagctacctt tgaccaccat ggcatcagcg gtgacggcct cccacttggg
    15301 ttccaggaac agccggagct gccgtccgcc ttcggtcttg ggttccgggc caagcactag
    15361 gccattaggc ccagccatgg ccaccagccc ttgcaggatg cgcagatcat cagcgcccag
    15421 cggctccggg ccgctgaact cgatccgctt gccgtcgccg tagtcatacg tcacgtccag
    15481 cttgctgcgc ttgcgctcgc cccgcttgag ggcacggaac aggccggggg ccagacagtg
    15541 cgccgggtcg tgtcggacgt ggctgaggct gtgcttgttc ttaggcttca ccacggggca
    15601 cccccttgct cttgcgctgc ctctccagca cggcgggctt gagcaccccg ccgtcatgcc
    15661 gcctgaacca ccgatcagcg aacggtgcgc catagttggc cttgctcaca ccgaagcgga
    15721 cgaagaaccg gcgctggtcg tcgtccacac cccattcctc ggcctcggcg ctggtcatgc
    15781 tcgacaggta ggactgccag cggatgttat cgaccagtac cgagctgccc cggctggcct
    15841 gctgctggtc gcctgcgccc atcatggccg cgcccttgct ggcatggtgc aggaacacga
    15901 tagagcaccc ggtatcggcg gcgatggcct ccatgcgacc gatgacctgg gccatggggc
    15961 cgctggcgtt ttcttcctcg atgtggaacc ggcgcagcgt gtccagcacc atcaggcggc
    16021 ggccctcggc ggcgcgcttg aggccgtcga accactccgg ggccatgatg ttgggcaggc
    16081 tgccgatcag cggctggatc agcaggccgt cagccacggc ttgccgttcc tcggcgctga
    16141 ggtgcgcccc aagggcgtgc aggcggtgat gaatggcggt gggcgggtct tcggcgggca
    16201 ggtagatcac cgggccggtg ggcagttcgc ccacctccag cagatccggc ccgcctgcaa
    16261 tctgtgcggc cagttgcagg gccagcatgg atttaccggc accaccgggc gacaccagcg
    16321 ccccgaccgt accggccacc atgttgggca aaacgtagtc cagcggtggc ggcgctgctg
    16381 cgaacgcctc cagaatattg ataggcttat gggtagccat tgattgcctc ctttgcaggc
    16441 agttggtggt taggcgctgg cggggtcact acccccgccc tgcgccgctc tgagttcttc
    16501 caggcactcg cgcagcgcct cgtattcgtc gtcggtcagc cagaacttgc gctgacgcat
    16561 ccctttggcc ttcatgcgct cggcatatcg cgcttggcgt acagcgtcag ggctggccag
    16621 caggtcgccg gtctgcttgt ccttttggtc tttcatatca gtcaccgaga aacttgccgg
    16681 ggccgaaagg cttgtcttcg cggaacaagg acaaggtgca gccgtcaagg ttaaggctgg
    16741 ccatatcagc gactgaaaag cggccagcct cggccttgtt tgacgtataa ccaaagccac
    16801 cgggcaacca atagcccttg tcacttttga tcaggtagac cgaccctgaa gcgctttttt
    16861 cgtattccat aaaaccccct tctgtgcgtg agtactcata gtataacagg cgtgagtacc
    16921 aacgcaagca ctacatgctg aaatctggcc cgcccctgtc catgcctcgc tggcggggtg
    16981 ccggtgcccg tgccagctcg gcccgcgcaa gctggacgct gggcagaccc atgaccttgc
    17041 tgacggtgcg ctcgatgtaa tccgcttcgt ggccgggctt gcgctctgcc agcgctgggc
    17101 tggcctcggc catggccttg ccgatttcct cggcactgcg gccccggctg gccagcttct
    17161 gcgcggcgat aaagtcgcac ttgctgaggt catcaccgaa gcgcttgacc agcccggcca
    17221 tctcgctgcg gtactcgtcc agcgccgtgc gccggtggcg gctaagctgc cgctcggaca
    17281 gttcgaggct ggccagcctg cgggccttct cctgctgccg ctgggcctgc tcgatctgct
    17341 ggccagcctg ctgcaccagc gccgggccag cggtggcggt cttgcccttg gattcacgca
    17401 gcagcaccca cggctgataa ccggcgcggg tggtgtgctt gtccttgcgg ttggtgaagc
    17461 ccgccaagcg gccatagtgg cggctgtcgg cgctggccgg gtcggcgtcg tactcgctgg
    17521 ccagcgtccg ggcaatctgc ccccgaagtt caccgcctgc ggcgtcggcc accttgaccc
    17581 atgcctgata gttcttcggg ctggtttcca ctaccagggc aggctcccgg ccctcggctt
    17641 tcatgtcatc caggtcaaac tcgctgaggt cgtccaccag caccagacca tgccgctcct
    17701 gctcggcggg cctgatatac acgtcattgc cctgggcatt catccgcttg agccatggcg
    17761 tgttctggag cacttcggcg gctgaccatt cccggttcat catctggccg gtggtggcgt
    17821 ccctgacgcc gatatcgaag cgctcacagc ccatggcctt gagctgtcgg cctatggcct
    17881 gcaaagtcct gtcgttcttc atcgggccac caagcgcagc cagatcgagc cgtcctcggt
    17941 tgtcagtggc gtcaggtcga gcaagagcaa cgatgcgatc agcagcacca ccgtaggcat
    18001 catggaagcc agcatcacgg ttagccatag cttccagtgc cacccccgcg acgcgctccg
    18061 ggcgctctgc gcggcgctgc tcacctcggc ggctacctcc cgcaactctt tggccagctc
    18121 cacccatgcc gcccctgtct ggcgctgggc tttcagccac tccgccgcct gcgcctcgct
    18181 ggcctgctgg gtctggctca tgacctgccg ggcttcgtcg gccagtgtcg ccatgctctg
    18241 ggccagcggt tcgatctgct ccgctaactc gttgatgcct ctggatttct tcactctgtc
    18301 gattgcgttc atggtctatt gcctcccggt attcctgtaa gtcgatgatc tgggcgttgg
    18361 cggtgtcgat gttcagggcc acgtctgccc ggtcggtgcg gatgccccgg ccttccatct
    18421 ccaccacgtt cggccccagg tgaacaccgg gcaggcgctc gatgccctgc gcctcaagtg
    18481 ttctgtggtc aatgcgggcg tcgtggcctg cccgctctaa tgcccggttg gcatggtcgg
    18541 cccatgcctc gcgggtctgc tcaagccatg ccttgggctt gagcgcttcg gtcttctgtg
    18601 ccccgccctt ctccggggtc ttgccgttgt accgcttgaa ccactgagcg gcgggccgct
    18661 cgatgccgtc attgatccgc tcggagatca tcaggtggca gtgcgggttc tcgccgccac
    18721 cggcatggat ggccagcgta tacggcaggc gctcggcacc ggtcaggtgc tgggcgaact
    18781 cggacgccag cgccttctgc tggtcgaggg tcagctcgac cggcagggca aattcgacct
    18841 ccttgaacag ccgcccattg gcgcgttcat acaggtcggc agcatcccag tagtcggcgg
    18901 gccgctcgac gaactccggc atgtgcccgg attcggcgtg caagacttca tccatgtcgc
    18961 gggcatactt gccttcgcgc tggatgaagt cggccttggc cctggccgat tggccgcccg
    19021 acctgctgcc ggttttcgcc gtaaggtgat aaatcgccat gctgcctcgc tgttgctttt
    19081 gcttttcggc tccatgcaat ggccctcgga gagcgcaccg cccgaagggt ggccgttagg
    19141 ccagtttctc gaagagaaac cggtaagtgc gccctcccct acaaagtagg gtcgggattg
    19201 ccgccgctgt gcctccatga tagcctacga gacagcacat taacaatggg gtgtcaagat
    19261 ggttaagggg agcaacaagg cggcggatcg gctggccaag ctcgaagaac aacgagcgcg
    19321 aatcaatgcc gaaattcagc gggtgcgggc aagggaacag cagcaagagc gcaagaacga
    19381 aacaaggcgc aaggtgctgg tgggggccat gattttggcc aaggtgaaca gcagcgagtg
    19441 gccggaggat cggctcatgg cggcaatgga tgcgtacctt gaacgcgacc acgaccgcgc
    19501 cttgttcggt ctgccgccac gccagaagga tgagccgggc tgaatgatcg accgagacag
    19561 gccctgcggg gctgcacacg cgcccccacc cttcgggtag ggggaaaggc cgctaaagcg
    19621 gctaaaagcg ctccagcgta tttctgcggg gtttggtgtg gggtttagcg ggctttgccc
    19681 gcctttcccc ctgccgcgca gcggtggggc ggtgtgtagc ctagcgcagc gaatagacca
    19741 gctatccggc ctctggccgg gcatattggg caagggcagc agcgccccac aagggcgctg
    19801 ataaccgcgc ctagtggatt attcttagat aatcatggat ggatttttcc aacaccccgc
    19861 cagcccccgc ccctgctggg tttgcaggtt tgggggcgtg acagttattg caggggttcg
    19921 tgacagttat tgcagggggg cgtgacagtt attgcagggg ttcgtgacag ttagtacggg
    19981 agtgacgggc actggctggc aatgtctagc aacggcaggc atttcggctg agggtaaaag
    20041 aactttccgc taagcgatag actgtatgta aacacagtat tgcaaggacg cggaacatgc
    20101 ctcatgtggc ggccctcgcg agaattaatt cagataaaaa aaatccttag ctttcgctaa
    20161 ggatgatttc tagcgatgac cctgctgatt ggttcgctga ccgtagactg gttggcttgg
    20221 tttcatcagc catccgcttg ccctcatctg ttacgccggc ggtagccggc cagcctcgca
    20281 gagcaggatt cccgttgagc accgccaggt gcgaataagg gacagtgaag aaggaacacc
    20341 cgctcgcggg tgggcctact tcacctatcc tgcccggctg acgccgttgg atacaccaag
    20401 gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg tgcgacatcg cgcgcgcgcg
    20461 cgcggatgct agcggcgcgc caaaaaaaag cccgctcatt a
//
