LOCUS       sequence-466014-       19839 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Eggerthella lenta A2 Dadh (Delta-Elen_0473) in 
            Gordonibacter urolithinfaciens DSM 27213.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19839)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 19839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
COMMENT     Sequence Label: pXD80ENSF7.3-Dadh-Delta-Elen0473(ElA2)
FEATURES             Location/Qualifiers
     source          1..19839
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..813)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     1300..1318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1475..1494
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             complement(3005..3028)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     terminator      13463..13490
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      13622..13649
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(14081..14932)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(14919..15758)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(16269..17240)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            18479..18566
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      18908..19302
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(19465..19508)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     oriT            19604..19713
                     /note="incP origin of transfer"
ORIGIN
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagatttag
     1501 tacatcagga ccactgggta gcccagctgg tagatcagca cgcgaaacat cacggaggtg
     1561 gccagcgcgc cgatggcggc gacgaccatg agcgtcttgg aggcgcccac cttcttcttc
     1621 gccacgaagg cgcaggccgc agccacgatc acggagacga tggagcccca gaacatgggc
     1681 gctcccgcgc cggacaccat gacggtcatc aggctgtcga cgtgcgtggg ggccgtggtc
     1741 atcgatgtcg ggtcggcgta gtagccgaat tgcgcgatct tcgccatggc gcaggcgatg
     1801 acgaacacca cgtcggccac gatctgcacg agggccgcga tgaacgtcag ctggatgccc
     1861 gtcgcctcga cggcctcgtc cttcttcagg atggagatca gccagacggc cgcggcgccc
     1921 aggaggcacg cgttgcccag atagaacgcc acgagcgcca ggccccacgc gggacgggcc
     1981 ggcatgaggt aggagtgcgc ggtggcgacc atcatgagca cggcaaccgc gagggtcgcc
     2041 caggccagcg ccttgggaac gggcttgccg cctcgaagga ccacgaacca tgcgacgatg
     2101 acgatggcca gcgccacgca tccgatgagc tcctgggtga tgcccgacgt gatgtgcccg
     2161 aagccgttga agatgcgctc ccagtgctcg aggtgcagga aggctccgat gccgcctacc
     2221 accagcgaga cggcggacgt gatgagcgcg gtcatctgca ggttcctgcc ctggcccttg
     2281 agcgccagga tggacatgcc gcccagcgtg ccgcaggtca agcatacgaa cagcgtgaag
     2341 atgacgagag gccactgcgt ttccattact catcacctcc ttcccagtga tggtcgcgta
     2401 ggatgtagga gtacgacggc ttgttgccca cgtcgggcag cttgtgcacg tcggcgtcgg
     2461 tgaacggctc caggaactcc accatcttgc ggcgcttgcc cagcgggtcc tcggagccct
     2521 cgaacgactc gtagccggcc tcggtgtcgc cgatccagcg ggcgttgccg ccgcactggc
     2581 tgacgcactg gggcagctcg ccctgcgaca gcttctgctc gcacagcgtg cacttctcga
     2641 cgaccttctc ctcctcgttg aggtagcgga cgccgtaggg gcaggccatg acgcagaact
     2701 ggcagccgat gcacttctcc ttgtcgatct ggatggagcc gtcctcggcc acgtgcgacg
     2761 cctcggtggg gcacaccttc acgcactcgg ggttctcgca gtgctggcag ctgatgggca
     2821 ggaagtacat gtacacgtcg gggtactgcc ctgatccgcc cgggatgggg ttcggcccca
     2881 cgcgcaccac tttgttccag tagctgccga tgggcacgtt gttgaccgcc ttgcaggcga
     2941 ccgagcacgc caggcagccc acgcaccggt tgaggtcggt gatgatagcc ttcgtcatgg
     3001 ttcagtgatg gtgatggtga tggtgatgct cgagtgcggc ctccctccct tcgtaggtag
     3061 gcagccattc cttcagacgg gggtcgtcgg aggtatggat gatgggcgtg ccggtggagt
     3121 cgcacggcac ggggttgttg aacggcgagt tctcgggcgt ggccttgtag atcttcacct
     3181 ggtaggcgcg caggttggac gtgccggagt gcgggtcctg ggcgtagtgg tcgatcagct
     3241 ggttcacctg ggagaactgc cagccgtggc cggcgtcctt cacctcgggg taccaccacg
     3301 tgtgctcgag gttgatgacg tcttccttca cgccgtagta caggtcagcc acttcgcgga
     3361 tcttgcccca ctcggtttcg atccacaccc agtcgccctg ctcgatgccg tactcggcgg
     3421 ccgtcttcgg gttgatctcc acgcgcggca cgggccacag ctcgcggcac cagggaagct
     3481 ggcgatgctc ggagtggaag tacaccggga tgcgacggcc ggtggtggcg gtcagcgggt
     3541 actccttgat gcggtcgccg tccttcgggc cgtgaggcgg ctcggtgtag gtgggaagcc
     3601 tccactcggg atggtcggga tggtgcgatt ccatgacggt ggaccagatc tcctgcttca
     3661 tcgtcggggt gaaccagccc ggcttccagt cgccgatgcc cttgccggcc gtgtagtcga
     3721 ggcggcccca cacgcggtcg cgtgcgcgca tcgcgccggt ctggtagcgg cggtacgtgc
     3781 cccagttgcg cggctcgatg tccttcgcct gccaccagcc gtgctcctgg aaggccttga
     3841 cgtactcgtc ccacgtcttg tacttgggcg tgacctcggc catcggaacg ccgtggcgct
     3901 cgaacgtggg cttgccgttc tcgacgtgcc acgtggtgta ggtcagctcg tcgtcggtga
     3961 gcagcttgat ggagtcgtcc agctgccagt tgatgtcggg ccactcgttg cccggctcgt
     4021 cggtccactt ccagttcatg cggcgggcga ggtccatgac gatctcggga tcgtacttgg
     4081 cttccgcggg cggctgcacg cacttgaccg tggcgcccat ggcgccggcg gaaccttggc
     4141 tggcgcgcgg cgagttgagc tcgatccaat gggccaccgg caggatgatg tcggcggtgt
     4201 cgttctgcgg cgtgtgccac aggttgaggt ccacgtagaa gtcaagcgag cacagggcct
     4261 cccaggcgcg cgtggtgttc gtctggctca tgaagttgcc ggactcgttc cagagggcgc
     4321 gcaccgggta ggggtcgccc gtgatgacgg cgtcccacag cgaatggctg tcgcaccagt
     4381 actgccacca gcccagaagc gggaacttgt cgatgccgat ctgcttgcgg ttgacttcct
     4441 ccggcggggt ggtggcgccg ggggcccagg cgctgaagcc ctggaggtcg ccgtcgatgg
     4501 ggacgatggt cgggccgcgg ttgccgcccg gggtgtccat gttgccggtg atgcccacga
     4561 ggttgtcgaa ggcgcggcag ttctggatgg cgctgcagaa gtgctcgacc gccagcatgt
     4621 actggatgcc gccgttgccg tagccggtgg acgggtcgat gcgcgtgccg taggccgtgg
     4681 ccgccgcctc gatctcggag gcggggatgc cggtgatctc ggccgccacc tcgggcttgt
     4741 actcggcggc gcgggccttg tagtgctccc acaccggctt gaccttcacg gtcttgccgt
     4801 ccttcagcgt gatctcgaac tcgccttcca gcgcggggtc gatggcgggg tcgaacggca
     4861 tgggatcggg cagccagccc tgcgtctggc ccggcagcag gttgtcctgc gcggcctcgc
     4921 ggccttggcg cgccttctcg gagtcgtagt tctcgccctc ccagaagccg ccggtctcgt
     4981 cgatgacgcc ttcctggtcg gcgttgaacc aggtgaacgc gccgtactcg tgctcgacgc
     5041 cctcggcctt cagcttctcc cagttgttgt cgtacacgag gaacttatag gggctgccgc
     5101 cctccttgat gtcgctttcc ttgagcagcg cggttttcac gtcccagtag gaaccgtcgg
     5161 tgcgcacggt ggggaagccg gagggctcca tgtcctcgca cacgaggaac ggggcgttcg
     5221 tccacttctt cacgtacagc tcgtcgtaga gcttcttctc gatgatgacg ttcgtccagg
     5281 ccagggccag ggcgccgtcg gtgccggggc gcaggtgctg ccagtagtcg gcttccttgc
     5341 ccatgttggc catgcggggg tcgacgctga tgtgcacgtc ggcgcgcgat gccacgtcca
     5401 ccgtggtgcg gcaggagtcg tcgtagttgg acagctcgga cgcgccgccc cactgcacgt
     5461 acactttcgg gcgcgtgatg gtttccatcc acgacatggc gaactgcgac accatggtgg
     5521 tggcgaagtg gcgcgggccc ttgcagatct gccaggcctc aacgttgttc ggcgtttcca
     5581 gcatgttctt caggatggac tccgagtgca tgcaccagat gcgcgacgtg cccacctggc
     5641 aggcgatggc ctcgccgccg tgcttcgcct tgatgtccat gaagttgtcc acgatggact
     5701 gcatggcctc gtcccacgag atgcgctgcc agccgggctc ctcgcccttc gggttcgtgc
     5761 gcttcatggg atagtgcaga cggtcggggt ggtacgccgc ctggatggac gactgcgact
     5821 tcgtgcagca gttgcccatg gactggaacg ctccctcgtc gccctcgacg cgtatggccc
     5881 gcccgttctg gacgatgacc ttgacgccgc actccatctt gccgcagccg cggcagcacg
     5941 ttttcacggc cacggtgtcg ttgccggaca cgctgctgta cgtctcttcg gccagcgcgg
     6001 tgtgcgtcga agcgccgaac gccgcggctg ccgcaccggt gatggcggcg gtcttgacga
     6061 acgtgcgtcg tgacatggtc aggttaccca tactcctcct ccttctttct cctcgttctc
     6121 gtcttgctcg tcttcgttcg tgcgatcggg gaggggggct gccgggtgaa gcctcgggct
     6181 ccgaaccggt gcaaagtccc cgtgtcgctt gggccgacca tacggatgcg agggggtgca
     6241 gagcatcgtc caaaggttgg aaagcaggtt cgaccgcatc ataaattctt atgatcttgt
     6301 gatcatgaaa aattcgatag tgctttgaac tggggaaact ttaaccgtag gtcataaatt
     6361 attctgaaac tagaatagtt tcctcaaaga gggccgctca tcttttccgt ataatcgaac
     6421 ccgtcgacag aggggtcggc gcgagcgacg ttatgtacgg gctcacaggg ggtggggcat
     6481 ggagacggcg tatctgcacg agttcgcgaa ggtggccgat tgcggcagct tcacggccgc
     6541 cgcgcgcgag ctgcatctga cgcagtcgac gctgagcaag cacgtggcct tgttggagcg
     6601 cgaattcggg gccgacctgt tcgtgcgcga ccgcagcggc gtgagcctga ccgaggcggg
     6661 cggcgtgctg tacgcccagg cgcggcagat ggagaagctg ctgcgcgcca ccgagctgct
     6721 ggtgcgcgcg gcgcgcgacg ggcagggttc ggcgggcgcg ggggttgcgg gcgcgacggc
     6781 gggcgcagcg gcgggcgggg gtgccgtcga cggctcgcct gcggccgcgt gcgacacggc
     6841 gcttcgctgc aagtgccggc tcgcggccga gcgctgcggg ctcgaccggc gcgagctggg
     6901 cgcgctcatc ctgtacgtgg aggagcgcgg cttcgaggcg atccagggcg agctggcgct
     6961 gacgcgcgac gaggtggccg acgtgctggg cagcgtgtac cgcaagctgg gcgtgggcga
     7021 caagcaggag gcgctcgatt tcatccattc cgtttcggaa tgatggtaga cgcgcccgcg
     7081 gcggttgggc atactgcgta gaagtgaaag cgctatgaag ctggggggct tcgagcaaag
     7141 ggcttcatcc gaagaatgga tggagaccga atatgagtgc ttggactgct gcgtccgtcg
     7201 aagacgcggc gaacaatctt cttgaagaac tgaatagcgg cgcgatgcgc ttgtgcgtgg
     7261 agcatgacgc gtccgacgac gttgcgccgg cgttgctgcg cgcggctcgc gctcgcgtcg
     7321 aggcggggtc gaacgtgcgc gtcgtgtgcg ccgacgccgt tgcagctgcg cgctttcggg
     7381 cgcttgcggc cgacgatccg ctgctgggcg cgctcgacgt ggtatcggtg cgcgagctgg
     7441 ccctctcgat tctaggcgac gcgcgggtcg gcgatgccgt gggacgcgac gcgcgcgtgc
     7501 tggacgagaa cgagctggcc gtgctcatgg aggacgtgaa ggtgagcggc ctcaagccgg
     7561 gccggctgcg cgagatgctc aagttcttct acaagagcat cagcgactgc gccgacgatg
     7621 acgagcgctg gctgatcacc gccgaagagc agaccgtgca cgccatcctc accgagaacc
     7681 tggaggcgcg ccgcgcgttg ctgccgtgcg aggtttcgtc gctggcgtat cgcggccttg
     7741 tgaaagccgg ggtcgagcgc gagccgctga cgctggtggc agacgacttc gggtcgctca
     7801 gcaaggcgtc gcagcgcctc gtgcgccatc tggcgacgga cggcctcgtg gcggcgggcc
     7861 gcacgctggc ctcgtccagc tccgaggagc cgtatccgtg cttcgacggg ttccgcgcgc
     7921 ttgccgacga ggccgactgc ctggtgacgc tggcgtgcga gcggccggcc gcggcgcgcg
     7981 agtcccgcgc gctggacgac ccggcggccg agttcgcgtt cgcggcggcg tccgtggagg
     8041 agtgcctggc cgacggcttc cggccaggcg acgtggctgt ggccgtgccg aacgccgctt
     8101 ggggcgagcg catcgccgcc gagctggaag ggcgcggcat cgccgtggag cgcgggttcg
     8161 acagcgggaa gatcaagggc gacccgcgca ccgagggccg ttacgccgac ctcaagctgg
     8221 ccgcgttcct gcggttgtac ctcgatccgc acgatttcac gtcgttgcgc tcgtggctgg
     8281 ggttcggcga ttggctgctg cgctccgatg cgtttttgga gctgatggcg tacgcgcgcg
     8341 accatgagac gaccgtgccc gaagccgtgg cgcagctgcg cacgcagcgc gacgccgatc
     8401 gctcctcgac gatattcggg aagttcgacg gcccgctcga cgagctggac gagctgctgc
     8461 gcgcctgcga ggaagggctg acgcgcgagg ccgccgtagc gctgttcgaa gcgcacggaa
     8521 tgccgttgtc cccgcgccac gtggagctgc tgggcgacga ccctgcgcat gcggacgtcg
     8581 agcgcttggc gcgctgcggg ttcgcgaagc cggtggagga tgtcgcgcgc gatgccgtgc
     8641 atgtggtctc ctacgcgcgc tgccacgggc accatgtgcg cacggtgttc gtcaccggcc
     8701 tggtgaacgg cttcttgccg gcgagcgacg ccgtggacga caagttcacc atcgaccatc
     8761 ggcgcgtggc gctcgaacgc gagcgcgcgc tgttcctgga cgtgctgtcc tgtgcgagcg
     8821 aacgagcggt atgcacgcgg ttcgtgcgcg accttttaga gaacaccgcg ttcacgaagg
     8881 tgcagacctc gcgcgtgttc gtgcgcgacg gcgagcgctt cgcgcgcgtg acgccgtcgg
     8941 agttcgcgtc gctgaccgac gaggtgctct ctcccgcgcc cgacgcgcct cgcgagctgc
     9001 cgacgaaggt gctgtacgcg tcttcgactt tgtagctcca acggtctgac gaccgcgcga
     9061 accttcgctg acttttcctg accggtgatt ttgaaatgat gaaaggactg atagatatga
     9121 gcgatgttga tgtattcgtg atcgatgacg atttcgatcc cctggccgac ctgagcctgg
     9181 acgacggcga gcgggacgat gccgaggcgg attacctgcc gcccatcccc gatgccgaca
     9241 agagcgtggt gcctcccgtg gtgccgttgt ctgcgaccga gcgcatcgag aagctgctgg
     9301 cgggcattcc cggccagcag ttccgcctgc tgcacgccgt ggaattctgc accgagccga
     9361 agacgatgga cgaggccgtg gccaatttgg acgcggcgta tccgaacacg acgagcgtgt
     9421 acggctcggc gcaggtggtg cagctgctcg agcgcgacgg cgcgctggag cgcatcgtgg
     9481 acgaggacgc cgcccatgcg gccgccggcg acgctgccga tacgcccgcc gaggacgagg
     9541 gcttcatctc ggtgacgccc gctccgccgt gtcactaccg tgccacgcaa gcgggcctcg
     9601 atgccgtcgc ggcgcacgtc aacgagggcc tcgtggcgga gcggatcagc gaagacgagc
     9661 gctatctgcc catcttccag cgcgtgcttg agatgtgcgc gcgcgaagga ggctgcccca
     9721 ccaaggagct ggaccaggcg gtggacggcg acccgctgtg ccaagagccg cgccgcttct
     9781 gcgggttctt ccgcggcaag ctggaagaaa caggcgccat cgaatggcgc gacgcctgga
     9841 ccattaccga tctggggcgc agcgtgctcg cgtccggctt gttcgccgcc gcttccccgt
     9901 ccgaacgcta gagaaggagc cacccatgtc ccacgatcag gaaaccacca tcgccgacgt
     9961 gctgcgcggc cgcatggcaa cctaccagtt cctctcgcgc ctgttccgcg tggaggtgga
    10021 ccaggagctg tacgacacgc tgatgtccat gcgcttcccg gcgaacaccg gcaacgcgct
    10081 ggtggacgaa ggctaccgga tgatctgcgg ttatctgagc caagccgacg gcacggtgct
    10141 caccgagctg gcggtggact acgtgcgcgc gttcatcggg tcgggcaacg acggcttctc
    10201 ggccgcgtac ccgtacgagt cggtgtacac gagcccgaag cgcctcatga tgcaggacgc
    10261 ccgcgacgag gtgctggtgc tgtaccgcgc cttcggtctg gacaagcagg aaagctggaa
    10321 ggagggcgag gaccatatcg cgctggagct ggagttcgag cagatcctat gcgagcgcgc
    10381 tatcagggcg tacgaggccg gcgacgagga cgagtgcctg aagctgctgc tgtcgcagcg
    10441 caacttcctg gaggaccacc tgctggcgtg gtaccccatg atggcggccg acttgcagaa
    10501 gttcccgcag accgacttct acaagggcct gggcaagctg accgacggct tcctgcgcaa
    10561 cgaccgcgaa tttttggacg ccgtgctgtc tgagaacgaa gcggattgcc atccagagcg
    10621 gaggccgcag gccgaagccg aaggatcccg tgcggcgtcg gccgaaaccg aagtcgaggt
    10681 tgcatgatgg acgagcacgg catcgagccc aagcgcgata cgggcccctg cgtgtcgcgc
    10741 cgcacgttcg ccctgggagc cgtgggagcg tgcgccgtca tcgggctggg cggcgtgaag
    10801 tacctgccca gtgccacgtt gctgcgcccg ccgggcgggc aggacgagga gcagctggcg
    10861 cgcggctgcc tgcattgcga gaagtgccgc gaggtgtgcc cgaagcatgc catcgctcct
    10921 gctcatcttg aggacggcat cttgaacgcg cgcacaccgc gcatggactt caaaagcggc
    10981 tggtgcgatt tctgcgagaa cgagccgggc gggccgaagt gcgtcgcggc gtgcccgacg
    11041 catgcgcttt cgtgccccga cccgtcgcag gcgatcatcg gcaaggcgga gatcaaccgc
    11101 gactggtgcc ttgccgcgaa gggcatgggc tgccacgagt gcgtcgacgt gtgcaactac
    11161 gaggcgctcg agctgggcgc cgacaacgtg ccggtggtgg acgtggatgc gtgcaacggc
    11221 tgcggcgcct gcgagctggc gtgcatctcg ctgtcggcag gttccatcac ggccggcgcc
    11281 accgatcgcg ccatcgtcgt ccgtccgacc gaagaagtgg aggcgtgatc aggatgaaga
    11341 agctcaaaac ctatcggctg cggttcctga tcatgctggc gctgctggcc gtggtggccg
    11401 tggggtactt cacggccggc ggcatcggca acttctgcgg catcggcttc gagtccatca
    11461 cgctgctgtg ccccctaggc gcgctgctgg ccatgatcgc cgagcgcaca gccatcccga
    11521 tggccgtgat cagcgttgcg gtcgtgctgc tggtgtgtat cgtgctaggc aaggtgttct
    11581 gcgcctgggc gtgccccgtg cacttcctgt cgcgaggaag gaagaagggg gataaggggg
    11641 acaaggaggg taaagggggc aaggttgctt ccccttgctc cgcgtgcgct tccccttgcg
    11701 gcaaagccaa gggcatcaag atcgacagcc gtcacggcat cctcgctgcg gcgttgggct
    11761 ccacgctcgt cttcggcttc ccggtgttct gcctcgtctg cccgatcggc ctcacgttcg
    11821 ccacggtgct gctggtcatg cgcctgttcg cgttcggcga cgccacgtgg acggtcgtcg
    11881 ccttcccggc catcatcgcg ctggagatcc tgctgctgcc gaagtggtgc cagcgcttct
    11941 gcccgttggg cgcgttgctt tcgctgttca gcgggctgaa ccggacgttc cgcccccagg
    12001 tggacgccga gaagtgcctg cgcgagggtc gcggcaaggc ctgcaacctg tgcgagcagg
    12061 catgccccga gggcatcaac ctgcacgatg tcgcggcggg cgagaccacg ctcaacgact
    12121 gcagcaagtg ccgcgcgtgc gccgacgtgt gccccgagcg cgccatcacg ttcccgttgc
    12181 tgccgaagaa gcaggcgcag gcgacgccgc tgcccgtcga cgacgagatc ccggaaaggg
    12241 gataggccat gtccctgatc aacggcctgc tggccctgtt gggcgagatc gagagcgatg
    12301 ccatcgccgt gcatgaggaa cgctgcatct cggttcgcaa ccgcaacgcc gactgcctgc
    12361 gctgcgtgga ggcatgcacg tcgggcgcgc tggcgtaccg cgcgggcgag ctgctcgtag
    12421 agcccgagcg ctgcatcgga tgcggcacgt gcgccacggc gtgccccacc tgcgccatcg
    12481 agctgcgcaa cccgacggat gccgagctga cggcgcagct caagcactcc atcgtggcga
    12541 cgaaaggcca tccggtcatc gtgtgcgagc aggcgctcga ggcggccaac gtcgcggccg
    12601 acgatgcgtc ggccgcgtgc gcggtgccgt gcctggggcg catggacgag tcggcgctcg
    12661 tggggttggc ggcgtaccgc gccttcgacg cgacgctggc ctgcggttcg tgcgagacgt
    12721 gcccacatgc tccgggcggc gcgctcgtgc gcgaggtggt ggagggcgcg cgcaacctgc
    12781 ttgaggcgtt cggatcctcc atgcccatcg acctggagga atgcgtgccc gagcgcgtgt
    12841 tggagcccgg cgggtcgcac ggccaggcag gcagcgacgg ggtggggcgg cgcgacttct
    12901 tccgctcggc caaggacgca tcgacccgcg cagctggcgc ggcggtggcc gaggagttgg
    12961 ggatggtcga ggccgagccc gttcctgcgg cgcatcgcaa agtgggcgca gatgggacgc
    13021 tgtcgcagtt cgtccccacg cgccgcgtgc ggctgtacaa ctacctgcag cacgtggggc
    13081 aaccgacggc cgacgaggtg gagacgcgtg tcatcggcgc cgtgtccatc gatgcccaga
    13141 agtgcagttc gtgccgcatg tgcgcggtgt tctgtcccac gggcgccatc aagaagctcg
    13201 acgagggcgg cgtgttcggc atcgtgcacc gtccgagcgc ctgcatgcag tgccgcctgt
    13261 gcgagcacat ctgtcccgag caggccatcg cggtgagcgg gcgcgtgccc atcaagcagt
    13321 tcatgggcaa ggaagccgtg tgcttcgcta tgaagcgtcc aacctggacc ccgaacaagc
    13381 cctcatcgat gtacgataag ctgcacgcga cgctcggcga acacttggag atgtgcatgt
    13441 tctagactag tcgactcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact
    13501 agagagagaa tataaaaagc cagattatta atccggcttt tttattattt aattcctgtt
    13561 ataaaaaaag gatcaatttt gaactctctc ccaaagttga tcccttaacg atccctgcag
    13621 gagaaggcca tcctgacgga tggccttttt gaagctttct agaatcgggg gcccccgggg
    13681 ggattctaga acaatggaag tcctcgtctc gccctcgaat tagcccgctt aatgagcggg
    13741 ctttttttga attaattctc gcgagctggc acgacaggtt tcccgactgg aaagcgggca
    13801 gtgagcgcaa cgcaattaat gtaagttagc gcgaattgca agctggccga cgcgctgggc
    13861 tacgtcttgc tggcgttcgg gagcagaaga gcatacatct ggaagcaaag ccaggaaagc
    13921 ggcctatgga gctgtgcggc agcgctcagt aggcaatttt tcaaaatatt gttaagcctt
    13981 ttctgagcat ggtatttttc atggtattac caattagcag gaaaataagc cattgaatat
    14041 aaaagataaa aatgtcttgt ttacaataga gtgggggggg tcagcctgcc gccttgggcc
    14101 gggtgatgtc gtacttgccc gccgcgaact cggttaccgt ccagcccagc gcgaccagct
    14161 ccggcaacgc ctcgcgcacc cgctggcggc gcttgcgcat ggtcgaacca ctggcctctg
    14221 acggccagac atagccgcac aaggtatcta tggaagcctt gccggttttg ccggggtcga
    14281 tccagccaca cagccgctgg tgcagcaggc gggcggtttc gctgtccagc gcccgcacct
    14341 cgtccatgct gatgcgcaca tgctggccgc cacccatgac ggcctgcgcg atcaaggggt
    14401 tcagggccac gtacaggcgc ccgtccgcct cgtcgctggc gtactccgac agcagccgaa
    14461 acccctgccg cttgcggcca ttctgggcga tgatggatac cttccaaagg cgctcgatgc
    14521 agtcctgtat gtgcttgagc gccccaccac tatcgacctc tgccccgatt tcctttgcca
    14581 gcgcccgata gctacctttg accaccatgg catcagcggt gacggcctcc cacttgggtt
    14641 ccaggaacag ccggagctgc cgtccgcctt cggtcttggg ttccgggcca agcactaggc
    14701 cattaggccc agccatggcc accagccctt gcaggatgcg cagatcatca gcgcccagcg
    14761 gctccgggcc gctgaactcg atccgcttgc cgtcgccgta gtcatacgtc acgtccagct
    14821 tgctgcgctt gcgctcgccc cgcttgaggg cacggaacag gccgggggcc agacagtgcg
    14881 ccgggtcgtg tcggacgtgg ctgaggctgt gcttgttctt aggcttcacc acggggcacc
    14941 cccttgctct tgcgctgcct ctccagcacg gcgggcttga gcaccccgcc gtcatgccgc
    15001 ctgaaccacc gatcagcgaa cggtgcgcca tagttggcct tgctcacacc gaagcggacg
    15061 aagaaccggc gctggtcgtc gtccacaccc cattcctcgg cctcggcgct ggtcatgctc
    15121 gacaggtagg actgccagcg gatgttatcg accagtaccg agctgccccg gctggcctgc
    15181 tgctggtcgc ctgcgcccat catggccgcg cccttgctgg catggtgcag gaacacgata
    15241 gagcacccgg tatcggcggc gatggcctcc atgcgaccga tgacctgggc catggggccg
    15301 ctggcgtttt cttcctcgat gtggaaccgg cgcagcgtgt ccagcaccat caggcggcgg
    15361 ccctcggcgg cgcgcttgag gccgtcgaac cactccgggg ccatgatgtt gggcaggctg
    15421 ccgatcagcg gctggatcag caggccgtca gccacggctt gccgttcctc ggcgctgagg
    15481 tgcgccccaa gggcgtgcag gcggtgatga atggcggtgg gcgggtcttc ggcgggcagg
    15541 tagatcaccg ggccggtggg cagttcgccc acctccagca gatccggccc gcctgcaatc
    15601 tgtgcggcca gttgcagggc cagcatggat ttaccggcac caccgggcga caccagcgcc
    15661 ccgaccgtac cggccaccat gttgggcaaa acgtagtcca gcggtggcgg cgctgctgcg
    15721 aacgcctcca gaatattgat aggcttatgg gtagccattg attgcctcct ttgcaggcag
    15781 ttggtggtta ggcgctggcg gggtcactac ccccgccctg cgccgctctg agttcttcca
    15841 ggcactcgcg cagcgcctcg tattcgtcgt cggtcagcca gaacttgcgc tgacgcatcc
    15901 ctttggcctt catgcgctcg gcatatcgcg cttggcgtac agcgtcaggg ctggccagca
    15961 ggtcgccggt ctgcttgtcc ttttggtctt tcatatcagt caccgagaaa cttgccgggg
    16021 ccgaaaggct tgtcttcgcg gaacaaggac aaggtgcagc cgtcaaggtt aaggctggcc
    16081 atatcagcga ctgaaaagcg gccagcctcg gccttgtttg acgtataacc aaagccaccg
    16141 ggcaaccaat agcccttgtc acttttgatc aggtagaccg accctgaagc gcttttttcg
    16201 tattccataa aacccccttc tgtgcgtgag tactcatagt ataacaggcg tgagtaccaa
    16261 cgcaagcact acatgctgaa atctggcccg cccctgtcca tgcctcgctg gcggggtgcc
    16321 ggtgcccgtg ccagctcggc ccgcgcaagc tggacgctgg gcagacccat gaccttgctg
    16381 acggtgcgct cgatgtaatc cgcttcgtgg ccgggcttgc gctctgccag cgctgggctg
    16441 gcctcggcca tggccttgcc gatttcctcg gcactgcggc cccggctggc cagcttctgc
    16501 gcggcgataa agtcgcactt gctgaggtca tcaccgaagc gcttgaccag cccggccatc
    16561 tcgctgcggt actcgtccag cgccgtgcgc cggtggcggc taagctgccg ctcggacagt
    16621 tcgaggctgg ccagcctgcg ggccttctcc tgctgccgct gggcctgctc gatctgctgg
    16681 ccagcctgct gcaccagcgc cgggccagcg gtggcggtct tgcccttgga ttcacgcagc
    16741 agcacccacg gctgataacc ggcgcgggtg gtgtgcttgt ccttgcggtt ggtgaagccc
    16801 gccaagcggc catagtggcg gctgtcggcg ctggccgggt cggcgtcgta ctcgctggcc
    16861 agcgtccggg caatctgccc ccgaagttca ccgcctgcgg cgtcggccac cttgacccat
    16921 gcctgatagt tcttcgggct ggtttccact accagggcag gctcccggcc ctcggctttc
    16981 atgtcatcca ggtcaaactc gctgaggtcg tccaccagca ccagaccatg ccgctcctgc
    17041 tcggcgggcc tgatatacac gtcattgccc tgggcattca tccgcttgag ccatggcgtg
    17101 ttctggagca cttcggcggc tgaccattcc cggttcatca tctggccggt ggtggcgtcc
    17161 ctgacgccga tatcgaagcg ctcacagccc atggccttga gctgtcggcc tatggcctgc
    17221 aaagtcctgt cgttcttcat cgggccacca agcgcagcca gatcgagccg tcctcggttg
    17281 tcagtggcgt caggtcgagc aagagcaacg atgcgatcag cagcaccacc gtaggcatca
    17341 tggaagccag catcacggtt agccatagct tccagtgcca cccccgcgac gcgctccggg
    17401 cgctctgcgc ggcgctgctc acctcggcgg ctacctcccg caactctttg gccagctcca
    17461 cccatgccgc ccctgtctgg cgctgggctt tcagccactc cgccgcctgc gcctcgctgg
    17521 cctgctgggt ctggctcatg acctgccggg cttcgtcggc cagtgtcgcc atgctctggg
    17581 ccagcggttc gatctgctcc gctaactcgt tgatgcctct ggatttcttc actctgtcga
    17641 ttgcgttcat ggtctattgc ctcccggtat tcctgtaagt cgatgatctg ggcgttggcg
    17701 gtgtcgatgt tcagggccac gtctgcccgg tcggtgcgga tgccccggcc ttccatctcc
    17761 accacgttcg gccccaggtg aacaccgggc aggcgctcga tgccctgcgc ctcaagtgtt
    17821 ctgtggtcaa tgcgggcgtc gtggcctgcc cgctctaatg cccggttggc atggtcggcc
    17881 catgcctcgc gggtctgctc aagccatgcc ttgggcttga gcgcttcggt cttctgtgcc
    17941 ccgcccttct ccggggtctt gccgttgtac cgcttgaacc actgagcggc gggccgctcg
    18001 atgccgtcat tgatccgctc ggagatcatc aggtggcagt gcgggttctc gccgccaccg
    18061 gcatggatgg ccagcgtata cggcaggcgc tcggcaccgg tcaggtgctg ggcgaactcg
    18121 gacgccagcg ccttctgctg gtcgagggtc agctcgaccg gcagggcaaa ttcgacctcc
    18181 ttgaacagcc gcccattggc gcgttcatac aggtcggcag catcccagta gtcggcgggc
    18241 cgctcgacga actccggcat gtgcccggat tcggcgtgca agacttcatc catgtcgcgg
    18301 gcatacttgc cttcgcgctg gatgaagtcg gccttggccc tggccgattg gccgcccgac
    18361 ctgctgccgg ttttcgccgt aaggtgataa atcgccatgc tgcctcgctg ttgcttttgc
    18421 ttttcggctc catgcaatgg ccctcggaga gcgcaccgcc cgaagggtgg ccgttaggcc
    18481 agtttctcga agagaaaccg gtaagtgcgc cctcccctac aaagtagggt cgggattgcc
    18541 gccgctgtgc ctccatgata gcctacgaga cagcacatta acaatggggt gtcaagatgg
    18601 ttaaggggag caacaaggcg gcggatcggc tggccaagct cgaagaacaa cgagcgcgaa
    18661 tcaatgccga aattcagcgg gtgcgggcaa gggaacagca gcaagagcgc aagaacgaaa
    18721 caaggcgcaa ggtgctggtg ggggccatga ttttggccaa ggtgaacagc agcgagtggc
    18781 cggaggatcg gctcatggcg gcaatggatg cgtaccttga acgcgaccac gaccgcgcct
    18841 tgttcggtct gccgccacgc cagaaggatg agccgggctg aatgatcgac cgagacaggc
    18901 cctgcggggc tgcacacgcg cccccaccct tcgggtaggg ggaaaggccg ctaaagcggc
    18961 taaaagcgct ccagcgtatt tctgcggggt ttggtgtggg gtttagcggg ctttgcccgc
    19021 ctttccccct gccgcgcagc ggtggggcgg tgtgtagcct agcgcagcga atagaccagc
    19081 tatccggcct ctggccgggc atattgggca agggcagcag cgccccacaa gggcgctgat
    19141 aaccgcgcct agtggattat tcttagataa tcatggatgg atttttccaa caccccgcca
    19201 gcccccgccc ctgctgggtt tgcaggtttg ggggcgtgac agttattgca ggggttcgtg
    19261 acagttattg caggggggcg tgacagttat tgcaggggtt cgtgacagtt agtacgggag
    19321 tgacgggcac tggctggcaa tgtctagcaa cggcaggcat ttcggctgag ggtaaaagaa
    19381 ctttccgcta agcgatagac tgtatgtaaa cacagtattg caaggacgcg gaacatgcct
    19441 catgtggcgg ccctcgcgag aattaattca gataaaaaaa atccttagct ttcgctaagg
    19501 atgatttcta gcgatgaccc tgctgattgg ttcgctgacc gtagactggt tggcttggtt
    19561 tcatcagcca tccgcttgcc ctcatctgtt acgccggcgg tagccggcca gcctcgcaga
    19621 gcaggattcc cgttgagcac cgccaggtgc gaataaggga cagtgaagaa ggaacacccg
    19681 ctcgcgggtg ggcctacttc acctatcctg cccggctgac gccgttggat acaccaagga
    19741 aagtctacac gaaccctttg gcaaaatcct gtatatcgtg cgacatcgcg cgcgcgcgcg
    19801 cggatgctag cggcgcgcca aaaaaaagcc cgctcatta
//
