LOCUS       sequence-466015-       19430 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Eggerthella lenta A2 Hcdh partial gene cluster (inactive) 
            in Gordonibacter urolithinfaciens DSM 27213.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19430)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 19430)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
COMMENT     Sequence Label: pXD80EN16-HcdhA(cHis8)BC-partial cluster(ElA2)
FEATURES             Location/Qualifiers
     source          1..19430
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..813)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     1300..1318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1475..1494
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             complement(6300..6323)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(6331..6342)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      13054..13081
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      13213..13240
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(13672..14523)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(14510..15349)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(15860..16831)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            18070..18157
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      18499..18893
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(19056..19099)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     oriT            19195..19304
                     /note="incP origin of transfer"
ORIGIN
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagatttac
     1501 ctctcgaaaa tgtctgatcc ttgcatgcga aggcgcatgg tgtccaccat ctgttgggcg
     1561 gtgcccaact cgaccggtcg cggcgtcatg gcatagcgat gcaccgcgcc ttcaagcaag
     1621 tacgcagggc gaacctcgtc gagcagctcg atggcgttct cgggacatac gtcgcgacag
     1681 ctgccgcact tcacgcattc gccgggatag tggtagacgc ccagcgttcc atcgtcgttg
     1741 tcgaatttgc ggatggcgcc cgtcgggcag aacgtcgcgc acatgcggca cgacgagcac
     1801 gcacgaccgt cgatcaccac gcaaccccac aggcgcgtcg caacgctggg cagtacgggc
     1861 tggcccagct gcgccagatt gtccaacagc gcctcgcgcc gatcgggaac gaaatggggc
     1921 aacgtgccgt ccttcgtaac gcgcggaagc gcagcgggag ccgcagccgc aggaacagcc
     1981 gacgaaaccg ccatgtgctc gttgccgcgg cgctcagcga agtacgccgc cacggcagcc
     2041 gctgccccat cagggccaac gccctcgaca caggcgccgg caggcacgcc ttcgaccacc
     2101 tgcacccgag catcgcttcc ccaggcttcg aacagggcgt tggacgttgc ggccaccagg
     2161 cgcgccgtgg caacgccgtg ctcctgctcg caacgcgagc agtcgccgca tgcaagccgg
     2221 atccggcgta cgccttcaac cgcaagcccg ctggtcagcg actcgtccac acgtcccaag
     2281 cacaccacgt tcgccacatg gccttcgtcg agcaacccgc ccgccgcacg ccgcagctgc
     2341 tcgcacgcca ccaccacttc gtcgccaacc cacgcgccca ggcacgcttg cgcaagctgc
     2401 gcatcgctgg ggttgcgcgc ttccagcgcg ctcgtggggc acacagtcgc gcacgtgccg
     2461 catccgatgc acttcgccgc gtccacccgc agctcgccgt ccaccagcgc gatacagccc
     2521 gacgtgcatg cgtcggcgca cttcaagcac gcgacgttgc ggttgcgcac tttcgcgcac
     2581 cgctcccggt gcaccacgat ggcctcgctg gacaccccgt ccagcacgcc tacgatactt
     2641 cggttcgacc gcgatcgctc gctcgcgcgc ccgtccatca ctcggccgtc ttctcgtcga
     2701 tgtcggccag cacgaccgtg cttgcctgag gccccgcacc cttcttcacc aaaaacggca
     2761 tgctgaccgc cttcgccgga cacgcgtcca cacaggctcg gcatcgcgtg cagtcggcca
     2821 gagtgcgctc gccgaaatcg ggatgccgca ggttgatatc ggcctcgcac gcgatggcgc
     2881 acttgctgca cgctgcgccg ttcaccgtct ccaggcactt cgactcgtcg atgaccggcc
     2941 gccacgtctt gctgaagcgc gacacgaggt tcatcagccc cgccatcggg cagaaccgcg
     3001 tgcaccactt gcgcaagaac accagctcca ccactagcaa cagcgggatg agcaccaccg
     3061 accacgtcat atcgcccgcc gcgaacaggc gccacaccac cagcacggtg gcgaacgtca
     3121 gcccgatggg gcacaccagg cagaacacgg gaaagccgaa gatggccgtg gacagcagcg
     3181 cgccgcccaa cacgtagtgg cgcgaatcca gcttcgcacg cacccccttg cacgacgagc
     3241 agctcccgca atcgtgagag tacgcgccgt cggccgctgt cggagatcct tcgactcgct
     3301 tcgctcgctc aggggatgcc gccgcctcgc ccagctcgct cttcgcgatg cccagcatct
     3361 ccgagcgctt cgcctgctca agcgccgtgc gcttcttgga cgagcggaag aacccgcgca
     3421 cgcggtcgag cagcggcacg gggcagaccc acccgcagaa cgcgcggccc accgccagca
     3481 cgagcaccgc catgatgacg atggacacca ccgcgcgcgg caccacggtc ttggtggcga
     3541 tcatggtggc cagcgccccc agcgggcaca acgccgagaa cgtctcccag ccgaagcccg
     3601 aaagcgtacc catgctcacg cccgcgatca gcccgacgga cagcacgacg aagatcgcgg
     3661 ccgccacgat ggtgcgcagc ttgttcgagt tcattgccgc ctcctattcc gccgactcga
     3721 tcggcttgac gacgatggcg cgctcctgcg cgcccgacac gatggacccc gccttcagcg
     3781 atacgcacac actctcgcat gcgccgcacc cgttgcactt gtcagccagc acggacacgt
     3841 gcgggttggc gccctcgccc gtcagctcta tggcctcgta ggggcacgcg tcgtagcagt
     3901 aacggcaccc cgtgtcgcgg aatgcaaggc actgcgcctc gtcgatcacg gccaaaccca
     3961 aaagcgtgtt ctccgccgta gcgtctgcgg gcagcgccag cgcctcggtg gggcacacct
     4021 tcacgcacaa cggctccccg ccgttctcgt cggcgcagta gtcgcagaaa tcggcgtcga
     4081 acttcagcgc gggcgagcgc atgcccagca gcccgtcctc gatatgcgca ggcacgatga
     4141 cgccgcgcgg gcacgcctcg tagcacttct cgcatcggat gcacgccgac acgaggcgcg
     4201 cctcgtcctg ccctcccggc gggcgcacca acgggttatg ccccgcatag cgcagcgccc
     4261 ccaatcccag cagcgccgcc gtgcttcccg cgccgatgag cagcgcgcgc cgcgacacgt
     4321 ccaacggccc cttcgccctc ttcgacgaaa tctcgtcctc ttcgctcatg tctcctcctt
     4381 tgcccactgg tccttatcag tcagcgaggt ttgctgtggt gccgcatatc ggcgcgtcgc
     4441 acggccgagc gtgcttcgca cgagaggggc acgtgcgaaa gcgtcgagat ggggtgccac
     4501 agcagaccgc agcgtcggcc ctttcgtcgt tcgacagaac gacgaaaggc agatgacggg
     4561 gcttccctgc cttgcgagaa gcggagctgt gccattcgag aagccccgtc catcacatct
     4621 gtttaagagc accggccgcg ctgagcggcg caaccggttc ggacgctcgg accaagatcg
     4681 accagaatct cggcccgagc gcgcccttcc gtctcctagt acaaagcgaa gatgctgata
     4741 gccaccacgt acaggatcac gcgccagcac aatccgccca cggcgacgca cgccagggcg
     4801 acgcccgcgt acgcggccag cttcttgccg gtttccacct tcttcgcaag ccacgtcagc
     4861 accagcggag caaccaggcc gacggccacc gtgcccagcc agaacatcac ggcattggca
     4921 ccggcgaaga tgctgcccac gatggacgcg cggtccacca tggccacgtc gggcagcgtc
     4981 ggatcgaagt agtactggat gtcggccgag tacagcccgg ccgagccgtt gatgatgaac
     5041 gcgtaggtga acacggcgat cagctgcagc gcgccgccgg ccaaggccac cttcacgccc
     5101 aggtccttcg cgctgtcatc gttcgtcaaa ccggcgatga ccagcgccgt caagccgccc
     5161 atgaacacgg tgttcgccaa gtagtacacc ggcagcagcg gggtgttcca cgacggcagt
     5221 gatgccatga ggtagctcat gcccgtgatc accggcagcg ccaggcccac gacgatggcc
     5281 agcacaccgc accatttcgg cgccatgccg tcttcggcgc ggcgcatcat caggaagtac
     5341 agcaccagca ccaccgcgaa cacgatgcag ccgatgaact ccagcgtgat acccgaggtg
     5401 atgtggccga agccgttgaa gatgcgctcc caatgctgca ggtgcatgaa caccgacagc
     5461 ccgccgacca ccaacgccgc cagcgcggtg accagcgatg caagctgcat cttcttgccc
     5521 ttgcccatga cgttcagcag gccttgcgcg cccagcgcgc cgcctgcgac gcataggaag
     5581 aaggtgaaga gaataagggg ccattcagga tgcatagtct actccctcca ctttcggccc
     5641 tcgcgcatga ggtacatgag cttcggctcg ttgcccacat ccgcaagatg gtggatatcg
     5701 ttttcggtgt acggctcgac gtagtccttc agcttcacgc gcgtctgggt catctcctcg
     5761 tactggcagc cggggctgtc gggatgcgcc ggcccctcga agttgtcgac gccctgatcg
     5821 aggtcgccga agaagcgggc gcgggcgccg cactgggcca cgcactgggg aagctcgccc
     5881 tgcgagatac gctgctcgca cagcgtgcat ttctccacga cgcgctcttc ctgattgagg
     5941 tagcgcacgc cgtacgggca cgccatcgcg cagaactggc agccgatgca cttcgacttg
     6001 tcgatttgca ccgtgccgtc gtcgcggatg tgggacgctt ccgtggggca caccttcacg
     6061 cattcggggt tctcgcagtg ctggcactgg acgggaagga agtacatctc gacgtccggg
     6121 aacgtgcccg agccgccctc gatgggatgc gggccgacgc gcagcgtctt gatccagaac
     6181 gatcccacgt cgacgccgtt gatggccttg cacgcaaccg tgcacgcaag gcagccggtg
     6241 caacggttca agtcggtaat gatcgcgtag tttgccatgt ggtgaacctc cttccttcag
     6301 tgatggtgat ggtgatggtg atgctcgagt gcggccttcg atttcgaata agcgcccagc
     6361 gcgtcgccgc ccacgctgcc cacggcaagc ttgccgccgg agagcaggtc ggggctttgg
     6421 acgctcggct ggcagccgac cgccgccatg ttcgcgaacg tgagctccac cttggaatcc
     6481 tccaggcgcg gatcgttcgc caaccattcc ttgaggcgcg gatcgtttgc gttcgtgatg
     6541 accggattgc cttgcgggtc gcacggaacc gggttgccat gcggcgagtt ctcgggcgtc
     6601 gcggggtaca cgagcaccgg cacgccgcgc agctgggacg cgccgcaaat ccaacactgc
     6661 gcgtacttgt ccatcgtgca gttgatgttc accagctcga agccgtgcga agcggtgtcg
     6721 atctcgggat accaccaggc gtggttcgcg ttgaccgtgc cctccttgat gccgtagtac
     6781 aagtccacga cctcgcggat ggcgccccac gggctgcgga tccaaatcca ctgcccctgc
     6841 tccaacccca ggcgcgctgc gtcgttgggg ttcatctcca aacgagggct gggccacagc
     6901 tcgcggcacc acggcagctg gcgatgctcg gagtggaagt acaccggctg gcgggagccc
     6961 gtcgtcatga tgaaggcgct gtcaggatgc gccgtcagcg cctccttgta ctcctccacc
     7021 agatgatcgc cctggtagtt gtcgttgatg taggcgtccg acgtcttgat ctggtcgacc
     7081 agcgaggcgt cgaagtactc ggggttcgat acgcgcgagt tcttcggctc cacccagtgg
     7141 gggaacttgt cgatgtcggg gatgttcgga tcgaccgtgt tcgtgctggc gaaggtggct
     7201 gcgccgtcgg ggatgtacgt ttcggcgatg gtggaccaca cctccacctt ggcagtgggg
     7261 gtcatgaagc cgcacttctc gtcgatggcc gcgtacaggt tgtagccgcc ctgctggcga
     7321 cggtagccca tctcccaacg gcggtacgtg ccccaacgct cggggtgcca cttgcggcag
     7381 tcgaaccagc cttcttcctg gaacttcgcc gcgtactgcg ggaagtcggg gccgtcgggg
     7441 tactcctcgg tcttccacca gtcggtggcg tccttgagca cgcggtactc ctgctcctcg
     7501 tagccgacgt tgccgcccat ctgcacgaag tcgtggtagt ccaggttgtt ccattcgtcg
     7561 tattcagggt cgcggttgtt ccacaccacg cccatggcct tgtacaggca gatgacggca
     7621 accgggtcgt agatgcaatc gcccatcggc tccacgcagc gctgcccggc accgaagatg
     7681 ccgcccgcgc cctgcgacac gcggcccgtg ttcacctcga gccagtgcag cacggggatc
     7741 acgatgtcgg cgcagccgtt gttcgggcag gaccacaggt tgaagtccag ccagaagtcc
     7801 agacgggtga gcgcctccca agcctccagc aggttggact cgttcatgaa gtcgcccgac
     7861 atgcacacgc cgccgtggat ctggtacggc gtgtcgatgc cgttgatgga gtcccagatg
     7921 gcagcggagt cggcccatcg gttccagtag cgcagcagcg ggaagcgctc ggcggaaatc
     7981 tgcttctcgt tgctgtcgaa cgacgtcttg agcgccggcc acgagcggct gggcttgtag
     8041 gcgccgccct tacgctcggc gatccagcgc gcctcttcgg cggtgggaac cttgttgccg
     8101 tagcgctcgg ccaagggcga tttctcgtcg atgaggtact gcacgaagtt ctggatcagc
     8161 gggatctggt cttccacgga caggtcgcgc ggggtgttgc ccagctccat ggtgcccacg
     8221 tccaagcccc agtccttcgg ctcatggtcg gtcaccagca tgttggcgcg gccgcagcag
     8281 ccgtccacct gggccttcga cgaaccgcgg ttgccggcgg gctcgtcgga gttgccggtg
     8341 atgcaggcga tcagctgcag cgcgcgggtg ttctgcaccg cgtggcccgt ctggtcggga
     8401 gcaagctggt agtggatgcc gccgttgccg tgcagcgggt tcaggcgcgt cgtgtagatg
     8461 cgcacgccct cctcgatctt gtcgaccggc acctcggtga cctcggacac gtactccagc
     8521 gtgtactgct ccaggctgtc ggagaacgcc tcccacaccg tgtcggcgct gatggtggag
     8581 ccgtccttca gcttcacctc cacgccgccg gggaacagcg ccgggttctt gggcagcccg
     8641 gccgggttcg agcgcttgcc gccctcgttg ccctcgtccc agtaggcgtc gtactgcgcg
     8701 tcggccggat cggcgaactt ggaggggtcg ggcagccagg cgtccgcgat gatgggcttg
     8761 tacggatgct cgatccaggt gcccgtcgtc gggatcttgt gcttctcgcc ctcccactgg
     8821 caggcctcgg catcccagta cgtaggcttg ttgttgttct cgtcccagca gatgaagcgc
     8881 tggtagcggc cctcgtagtc ccagtactgg ccgatccact cggggtcgca gtcggcctcg
     8941 gtcaggatgc ggctctccat gtcgatgccg ccgttcattt ccatgaacca acccttcgcc
     9001 gtgcggccgt ccttctcggg gttccacaag aacggggcgt tcgtccaacg gcgcacgaac
     9061 gcgtcgtcgt acgcctcgtt gtcgaggatc cacttgagcc aacccagcga caggcacagg
     9121 tcggtgccca cgcgcagcgg cagccacacg tcggcttcct tgcccagcgg cgtcatgcgg
     9181 ggatccacca ggatgtgctt gtaggcgcgc tgcgagcagt ccaccaccgt acggttcgtg
     9241 gagtcgtagt tcgagtactc cgacgcggtg ccccactgca cgtacaccag cgggccctgc
     9301 tcaacctcca tccacggcga gccgatctcg tcggtgagga tgccgccgaa gtggcgcgga
     9361 cccttgcaga tctcgtaggc caggtgcgcg ttcggcgtgg ggaagatgga cttcagcgtg
     9421 ccgtacggcg gctgcgccca cacgcgcgag gtgccgccca tggcgaagtt ggcttccccg
     9481 ccgtacttct ccacgatctc cttgaacttg gcacccgcct cgtcgaacgc ctcggcaagc
     9541 gtgatgcgca cccaaccggg gtcgtcctcg cctttggggt tcgtgcgctt catgcagtag
     9601 cgcagacgat cgggatggta cagagccagc atggacgact gcgacttcgc gcagcagtga
     9661 ccgcggctgt gagcgttgga ctcgtcgccc tcgaccttga tgaccttgtt gtcctgaacg
     9721 gtcacccaca cgccgcactc caccttgccg cacgcgcggc agcaggagcg gatgcgcttg
     9781 atctcgcccg ctgaggcgtc ttcaccttcg gccaacgccg tcgaaggcgt tgctgcgaag
     9841 cccaccgtgg ttgccgcggc agccatggca gaggccttca agaagcttcg acgactcagt
     9901 gataggttcg ccatgtgccc ctcctctctt ccttttctcg cttcgttcgt ctctgccgga
     9961 acccggcgcg accatacgcc ggaaccggtt tccccattct aggaacggag caaaagccca
    10021 aatcataagg ggtggatgga tgaaacgcgc cgatatcatc cccaaaggat tattttgctt
    10081 ttcgcgccca acctatactt gatggcagcg aagaggttgc cgttcgcgcc cgtcatccgg
    10141 gcgtgcggca acgggaagaa acctgtgaac gaaggagact gccatggcac aaccgcgtcc
    10201 gtcattgacg ctgatcctcg acctcgacga gcgcctcgac agcgaggacg tgcgcctgga
    10261 aatcgaccgc tgctactcgt acgtagggtc gacactggtg cgcacccatc ccgcctgcga
    10321 cggcgagccg cagaacatca tgcgcttcct cgtgaagctg ggcacgcgcc gctacctgcg
    10381 cgccgaggac gagggcgccg acgagctgtg gaacgacgtt atggagcgct ggttctacaa
    10441 cgagctgtac aaggtgagta acaacatgct gatctacaac cgccgccagc gcgaagtggg
    10501 caacccccag ctggtgttcg actggatcga cgtggagctg cagaacggcc agctgcatgc
    10561 gctgctgcat tgcgacaacg tcagcggcat ccgacccgag accagcgagc ttctgacgca
    10621 gctgcgcgca gcgtacaacg aaggcgcgct gggcgaggac gtcgtgcgcg cctacctgcc
    10681 cgcgcccgct tcatacgagg aacagaaggc cgccgggctg gcggcgaagg ccgagcgcga
    10741 cgcgcagaag gcggcggggc tggctgcggc agaagaggag gcccgggctg cggccgcggc
    10801 ggccgaagcc gcggcagagg aggcgtttct ggagctgccg cgtttggcca acgacgccgc
    10861 cgaagaggag gacgagcccg ctcttgagcc gttcgccctg gacgagcccg acttcgaggt
    10921 cgactaccgc ttgtggctga tcgaatacgc cgacggcagc acgcgcacgt tcgactcgca
    10981 cgccggaacc ctcgcgtaac cgaaaaggag ccttaccatg atcgaagcca tcgcctcctt
    11041 cgacgggcgc atcgccaagg tgcaaggacc tgcgcgaagc ggcaagaccg aagcgctcgt
    11101 tcggcgttgc gcctgcctcg tgcgcggcgg cgccgccccg gaaaccatcc tcgtggaaac
    11161 gtcgagcgcc gcagcggctc aggcgttccg ccgtcggctt cgccgcgcgc tcggccccga
    11221 cctccagcac gccgccgacg acgtgcatgt gcgcaccgcg ctcgaaacgt gcgtcgccgt
    11281 gctggacgcg cccgccgcgc gcgccgccac cggccgcgtg ccgcgcctgt tgaacgatgc
    11341 ggagtacaac ttcttcctgg aggatatgaa gacgctcggc cagcccatcc gccgtttgcg
    11401 caaaatgctc gatttcttct atcgccagat gagcgacctc gagccgcgcg actcctggct
    11461 cgcaggcggc gaagaggaag ccgtgctgtc ccacctcgag cgcgtgctgg cctcacgcgg
    11521 cgccatgctg gcgcaggaag cgcccagcct gtgcgccgcg tacctgcgca gcgacgcggg
    11581 cgagggcgtg tgcggcagct acgcctacgt gctgtgcgac gacttccaga acatgagccg
    11641 cgccgagcag acgtgcctgt gcctgctggc cgaccgccag ctcatcgcgt gcggcaaccc
    11701 caaccagcag caggccgcgc gcgcgtcgtt cccctgcgcc gagggcttcg tgcagttcga
    11761 cgctcgccgc cgcgacgtag ccgtgttcac gctgtccgga gcgcacggca accccgccat
    11821 cgccgcgttc gccgacgggc tttgcgacca gggcgacatg gatcccgcct tcaaagcggg
    11881 catcgcgagc gacgcgcaga cgtcggacgg catcatggcc gtcaagtggt cgacgccgga
    11941 agacgagctg gacggcatca cgaagtacct gcgcattctg ctcgacggcg aagaggacct
    12001 gcacgagaac cgcacgtgcg tgctcgtgcc caacaagcgc tgggcgctca tggcgcagca
    12061 ggtgctcaag cagcgcggtt tcgccgtggc gctggccgga gcgttctccc gtttgggcgg
    12121 cgacccgcgc gacagcgccc gcgcccgcgc gctcgtggcg tacacgaagc tcaacctgct
    12181 ggccgatccg cgcgacatga cggcttggcg cagctggtgc ggcttcgaca actacctcac
    12241 gaacagcgat gcatggggcg gcttgcaggc tttcgccgaa aagcgcgagc tcacgctcta
    12301 cgacgcgctc gccctggccg accagctgga agacgaaccg ttcctgcgcg cgcacgccct
    12361 ggtcgagcgc tggcgcgcgg gccaggagct tatcgccgcg aacgccgggc gcaagggctt
    12421 cggcctgctg cacgccatcg gagccgacga tctgcccgag ttcgaggaga cggcgcgcgc
    12481 cctcgtgggc gacgaggatg cggcagcgat gttcgccctg cagcgcacca acgtcaccga
    12541 tcccgcgctg cccgacgatc cgcacgtgct gcacgtggcc tcctacggcg ccctgtgcgg
    12601 catcgaatac gacaacatct tcgccatcgc cgccgtggac ggcttcatgc cgcggcgcga
    12661 tgcgttcgag gtgatcagca ccgaggaaga ccgcgagcgc atcatgaacg aagagcgccg
    12721 attgttctgc aacagcgtgt cgaaggccag caaacgcctg gtcgtctcct atttcagcaa
    12781 agcgccgctc gagcttgccg agcgtgccaa gatgcaggtg gtgcgcgtga aggccgaggg
    12841 caacggccgc gtggcctcgg tgcgtcccac cgcgtttttg gccgaagcgg gaaacgccgc
    12901 ccccgtcacc accggcggtc aagccctcct cgcccagcac gggctgaact agccgccaac
    12961 gcgccgccgc ccagcggcgg cgcgaagctg ccttcgtacc ggcgctccag accaagaaga
    13021 gccttccgac gcgcggacta gtcgactcac actggctcac cttcgggtgg gcctttctgc
    13081 gtttatatac tagagagaga atataaaaag ccagattatt aatccggctt ttttattatt
    13141 taattcctgt tataaaaaaa ggatcaattt tgaactctct cccaaagttg atcccttaac
    13201 gatccctgca ggagaaggcc atcctgacgg atggcctttt tgaagctttc tagaatcggg
    13261 ggcccccggg gggattctag aacaatggaa gtcctcgtct cgccctcgaa ttagcccgct
    13321 taatgagcgg gctttttttg aattaattct cgcgagctgg cacgacaggt ttcccgactg
    13381 gaaagcgggc agtgagcgca acgcaattaa tgtaagttag cgcgaattgc aagctggccg
    13441 acgcgctggg ctacgtcttg ctggcgttcg ggagcagaag agcatacatc tggaagcaaa
    13501 gccaggaaag cggcctatgg agctgtgcgg cagcgctcag taggcaattt ttcaaaatat
    13561 tgttaagcct tttctgagca tggtattttt catggtatta ccaattagca ggaaaataag
    13621 ccattgaata taaaagataa aaatgtcttg tttacaatag agtggggggg gtcagcctgc
    13681 cgccttgggc cgggtgatgt cgtacttgcc cgccgcgaac tcggttaccg tccagcccag
    13741 cgcgaccagc tccggcaacg cctcgcgcac ccgctggcgg cgcttgcgca tggtcgaacc
    13801 actggcctct gacggccaga catagccgca caaggtatct atggaagcct tgccggtttt
    13861 gccggggtcg atccagccac acagccgctg gtgcagcagg cgggcggttt cgctgtccag
    13921 cgcccgcacc tcgtccatgc tgatgcgcac atgctggccg ccacccatga cggcctgcgc
    13981 gatcaagggg ttcagggcca cgtacaggcg cccgtccgcc tcgtcgctgg cgtactccga
    14041 cagcagccga aacccctgcc gcttgcggcc attctgggcg atgatggata ccttccaaag
    14101 gcgctcgatg cagtcctgta tgtgcttgag cgccccacca ctatcgacct ctgccccgat
    14161 ttcctttgcc agcgcccgat agctaccttt gaccaccatg gcatcagcgg tgacggcctc
    14221 ccacttgggt tccaggaaca gccggagctg ccgtccgcct tcggtcttgg gttccgggcc
    14281 aagcactagg ccattaggcc cagccatggc caccagccct tgcaggatgc gcagatcatc
    14341 agcgcccagc ggctccgggc cgctgaactc gatccgcttg ccgtcgccgt agtcatacgt
    14401 cacgtccagc ttgctgcgct tgcgctcgcc ccgcttgagg gcacggaaca ggccgggggc
    14461 cagacagtgc gccgggtcgt gtcggacgtg gctgaggctg tgcttgttct taggcttcac
    14521 cacggggcac ccccttgctc ttgcgctgcc tctccagcac ggcgggcttg agcaccccgc
    14581 cgtcatgccg cctgaaccac cgatcagcga acggtgcgcc atagttggcc ttgctcacac
    14641 cgaagcggac gaagaaccgg cgctggtcgt cgtccacacc ccattcctcg gcctcggcgc
    14701 tggtcatgct cgacaggtag gactgccagc ggatgttatc gaccagtacc gagctgcccc
    14761 ggctggcctg ctgctggtcg cctgcgccca tcatggccgc gcccttgctg gcatggtgca
    14821 ggaacacgat agagcacccg gtatcggcgg cgatggcctc catgcgaccg atgacctggg
    14881 ccatggggcc gctggcgttt tcttcctcga tgtggaaccg gcgcagcgtg tccagcacca
    14941 tcaggcggcg gccctcggcg gcgcgcttga ggccgtcgaa ccactccggg gccatgatgt
    15001 tgggcaggct gccgatcagc ggctggatca gcaggccgtc agccacggct tgccgttcct
    15061 cggcgctgag gtgcgcccca agggcgtgca ggcggtgatg aatggcggtg ggcgggtctt
    15121 cggcgggcag gtagatcacc gggccggtgg gcagttcgcc cacctccagc agatccggcc
    15181 cgcctgcaat ctgtgcggcc agttgcaggg ccagcatgga tttaccggca ccaccgggcg
    15241 acaccagcgc cccgaccgta ccggccacca tgttgggcaa aacgtagtcc agcggtggcg
    15301 gcgctgctgc gaacgcctcc agaatattga taggcttatg ggtagccatt gattgcctcc
    15361 tttgcaggca gttggtggtt aggcgctggc ggggtcacta cccccgccct gcgccgctct
    15421 gagttcttcc aggcactcgc gcagcgcctc gtattcgtcg tcggtcagcc agaacttgcg
    15481 ctgacgcatc cctttggcct tcatgcgctc ggcatatcgc gcttggcgta cagcgtcagg
    15541 gctggccagc aggtcgccgg tctgcttgtc cttttggtct ttcatatcag tcaccgagaa
    15601 acttgccggg gccgaaaggc ttgtcttcgc ggaacaagga caaggtgcag ccgtcaaggt
    15661 taaggctggc catatcagcg actgaaaagc ggccagcctc ggccttgttt gacgtataac
    15721 caaagccacc gggcaaccaa tagcccttgt cacttttgat caggtagacc gaccctgaag
    15781 cgcttttttc gtattccata aaaccccctt ctgtgcgtga gtactcatag tataacaggc
    15841 gtgagtacca acgcaagcac tacatgctga aatctggccc gcccctgtcc atgcctcgct
    15901 ggcggggtgc cggtgcccgt gccagctcgg cccgcgcaag ctggacgctg ggcagaccca
    15961 tgaccttgct gacggtgcgc tcgatgtaat ccgcttcgtg gccgggcttg cgctctgcca
    16021 gcgctgggct ggcctcggcc atggccttgc cgatttcctc ggcactgcgg ccccggctgg
    16081 ccagcttctg cgcggcgata aagtcgcact tgctgaggtc atcaccgaag cgcttgacca
    16141 gcccggccat ctcgctgcgg tactcgtcca gcgccgtgcg ccggtggcgg ctaagctgcc
    16201 gctcggacag ttcgaggctg gccagcctgc gggccttctc ctgctgccgc tgggcctgct
    16261 cgatctgctg gccagcctgc tgcaccagcg ccgggccagc ggtggcggtc ttgcccttgg
    16321 attcacgcag cagcacccac ggctgataac cggcgcgggt ggtgtgcttg tccttgcggt
    16381 tggtgaagcc cgccaagcgg ccatagtggc ggctgtcggc gctggccggg tcggcgtcgt
    16441 actcgctggc cagcgtccgg gcaatctgcc cccgaagttc accgcctgcg gcgtcggcca
    16501 ccttgaccca tgcctgatag ttcttcgggc tggtttccac taccagggca ggctcccggc
    16561 cctcggcttt catgtcatcc aggtcaaact cgctgaggtc gtccaccagc accagaccat
    16621 gccgctcctg ctcggcgggc ctgatataca cgtcattgcc ctgggcattc atccgcttga
    16681 gccatggcgt gttctggagc acttcggcgg ctgaccattc ccggttcatc atctggccgg
    16741 tggtggcgtc cctgacgccg atatcgaagc gctcacagcc catggccttg agctgtcggc
    16801 ctatggcctg caaagtcctg tcgttcttca tcgggccacc aagcgcagcc agatcgagcc
    16861 gtcctcggtt gtcagtggcg tcaggtcgag caagagcaac gatgcgatca gcagcaccac
    16921 cgtaggcatc atggaagcca gcatcacggt tagccatagc ttccagtgcc acccccgcga
    16981 cgcgctccgg gcgctctgcg cggcgctgct cacctcggcg gctacctccc gcaactcttt
    17041 ggccagctcc acccatgccg cccctgtctg gcgctgggct ttcagccact ccgccgcctg
    17101 cgcctcgctg gcctgctggg tctggctcat gacctgccgg gcttcgtcgg ccagtgtcgc
    17161 catgctctgg gccagcggtt cgatctgctc cgctaactcg ttgatgcctc tggatttctt
    17221 cactctgtcg attgcgttca tggtctattg cctcccggta ttcctgtaag tcgatgatct
    17281 gggcgttggc ggtgtcgatg ttcagggcca cgtctgcccg gtcggtgcgg atgccccggc
    17341 cttccatctc caccacgttc ggccccaggt gaacaccggg caggcgctcg atgccctgcg
    17401 cctcaagtgt tctgtggtca atgcgggcgt cgtggcctgc ccgctctaat gcccggttgg
    17461 catggtcggc ccatgcctcg cgggtctgct caagccatgc cttgggcttg agcgcttcgg
    17521 tcttctgtgc cccgcccttc tccggggtct tgccgttgta ccgcttgaac cactgagcgg
    17581 cgggccgctc gatgccgtca ttgatccgct cggagatcat caggtggcag tgcgggttct
    17641 cgccgccacc ggcatggatg gccagcgtat acggcaggcg ctcggcaccg gtcaggtgct
    17701 gggcgaactc ggacgccagc gccttctgct ggtcgagggt cagctcgacc ggcagggcaa
    17761 attcgacctc cttgaacagc cgcccattgg cgcgttcata caggtcggca gcatcccagt
    17821 agtcggcggg ccgctcgacg aactccggca tgtgcccgga ttcggcgtgc aagacttcat
    17881 ccatgtcgcg ggcatacttg ccttcgcgct ggatgaagtc ggccttggcc ctggccgatt
    17941 ggccgcccga cctgctgccg gttttcgccg taaggtgata aatcgccatg ctgcctcgct
    18001 gttgcttttg cttttcggct ccatgcaatg gccctcggag agcgcaccgc ccgaagggtg
    18061 gccgttaggc cagtttctcg aagagaaacc ggtaagtgcg ccctccccta caaagtaggg
    18121 tcgggattgc cgccgctgtg cctccatgat agcctacgag acagcacatt aacaatgggg
    18181 tgtcaagatg gttaagggga gcaacaaggc ggcggatcgg ctggccaagc tcgaagaaca
    18241 acgagcgcga atcaatgccg aaattcagcg ggtgcgggca agggaacagc agcaagagcg
    18301 caagaacgaa acaaggcgca aggtgctggt gggggccatg attttggcca aggtgaacag
    18361 cagcgagtgg ccggaggatc ggctcatggc ggcaatggat gcgtaccttg aacgcgacca
    18421 cgaccgcgcc ttgttcggtc tgccgccacg ccagaaggat gagccgggct gaatgatcga
    18481 ccgagacagg ccctgcgggg ctgcacacgc gcccccaccc ttcgggtagg gggaaaggcc
    18541 gctaaagcgg ctaaaagcgc tccagcgtat ttctgcgggg tttggtgtgg ggtttagcgg
    18601 gctttgcccg cctttccccc tgccgcgcag cggtggggcg gtgtgtagcc tagcgcagcg
    18661 aatagaccag ctatccggcc tctggccggg catattgggc aagggcagca gcgccccaca
    18721 agggcgctga taaccgcgcc tagtggatta ttcttagata atcatggatg gatttttcca
    18781 acaccccgcc agcccccgcc cctgctgggt ttgcaggttt gggggcgtga cagttattgc
    18841 aggggttcgt gacagttatt gcaggggggc gtgacagtta ttgcaggggt tcgtgacagt
    18901 tagtacggga gtgacgggca ctggctggca atgtctagca acggcaggca tttcggctga
    18961 gggtaaaaga actttccgct aagcgataga ctgtatgtaa acacagtatt gcaaggacgc
    19021 ggaacatgcc tcatgtggcg gccctcgcga gaattaattc agataaaaaa aatccttagc
    19081 tttcgctaag gatgatttct agcgatgacc ctgctgattg gttcgctgac cgtagactgg
    19141 ttggcttggt ttcatcagcc atccgcttgc cctcatctgt tacgccggcg gtagccggcc
    19201 agcctcgcag agcaggattc ccgttgagca ccgccaggtg cgaataaggg acagtgaaga
    19261 aggaacaccc gctcgcgggt gggcctactt cacctatcct gcccggctga cgccgttgga
    19321 tacaccaagg aaagtctaca cgaacccttt ggcaaaatcc tgtatatcgt gcgacatcgc
    19381 gcgcgcgcgc gcggatgcta gcggcgcgcc aaaaaaaagc ccgctcatta
//
