LOCUS       sequence-466016-       20550 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Eggerthella lenta A2 (–)-Eldh2 in Gordonibacter 
            urolithinfaciens DSM 27213.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20550)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 20550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
COMMENT     Sequence Label: pXD80ENSF1-C1858_02810-(–)-Eldh2-Accs (ElA2)
FEATURES             Location/Qualifiers
     source          1..20550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..813)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     1300..1318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1475..1494
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      14174..14201
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      14333..14360
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(14792..15643)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(15630..16469)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(16980..17951)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            19190..19277
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      19619..20013
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(20176..20219)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     oriT            20315..20424
                     /note="incP origin of transfer"
ORIGIN
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagatctac
     1501 tgctcgcttt tgcgatcctt acgccggtcg agaagagttt gggcctcttc tgccgcagcg
     1561 aagaaatcgc gtaagcgttc ttcgtttcta ggatggtaga cggcgtatgc tacgaggttc
     1621 ttgtcttttt cctcgatcgg tataccggtg cgctttccgg tttccagcag taagccgatc
     1681 tgcttttgcg tctttttcca tatgaggacg ttgcctctga gcggtgtgct gaagaactcc
     1741 acgttgttgc ctgccgatac gggcctggtc ttgggctcga atccgtaatc gatacatgcg
     1801 gcttcgattt gatcccaggc agggttcgtc ttgtcgctca tgaatttcac cagcgtttgc
     1861 gtttgcagat ctttccacga gatcgaggtt cggttggcaa gcggatggtc gttttctacg
     1921 attgcgagca attgaatcga cacgaacggc ctactgatca atccctcttg ttcggcgtga
     1981 cgggggttgg tgtatccgat gaacaaatcg atggactctc tcgatagcaa cgtgaagggg
     2041 tcgtcgatgt tgcggtcgaa aacgatgttc gcgtgatgat tttcgcgggc gatcattgcc
     2101 gtcatagaag cgagggaggc tatgtcggag tcttccatgt gtccgaccac gtgaataggg
     2161 cgcttcgctt tgagctgttc gagcgtgttt ctggtcttgt cgtattcgtt cacgatgacc
     2221 ccggcgcttt cgaacaagat gcgtccgcct tcggttagct gaactccttt tttccctcga
     2281 tcaagcagtt ctattccgaa ttctttttca agggcgagca tgtgcttgct caatgccggt
     2341 tgggtgatgt tgaggtttcc ggccgcctcg gtgaagttca gacattgagc gagctcgatg
     2401 aattctctgc aggattcgat gttcatgatg accccccccc tccgccgaat gaaaacccat
     2461 tcggcgattg cattgcgagg ttcgatgccc ccccagcatc gctcgtctta aaagccattg
     2521 tagcatcctg ggtgattttg agaggtacac gcgctttcct taggcctgtt ggcatggtct
     2581 tataacgcat cggaatggga ggggcttgaa tcggaatgca tgcggggcga tgtgtgccga
     2641 aacgttattt ccctatcttc gagttgcttt tcatactgaa ggaagatgcg atctccttaa
     2701 tggggtcgtc gtgggcaggg tcgaaaataa ggaggagggt atgggtaaac tgcatatgtc
     2761 gcgacgggat ttcgtcaaag tctcggtcgt gacaggcgcg gcgttcggca tctccgcgtt
     2821 ggctgagccg agtttggctg ttgctgacga tgccgtcgat aagccggtgg gaggtgacat
     2881 caagcgtatc cgaacagggt gtcgcggctg cggcaaaatg gaatgcggcg ttatcgccgt
     2941 agtgcagaac gggcgtgtcg ttcggatgga gggcgaccct acggcgttcc agtcgatggg
     3001 gaactgctgc accaaatcgc aagcggccat tcaggcggca taccatcctg atcgtctgca
     3061 ttatcctatg aaacgaacga acgacaagga atccgatgat cccggttggg tgcgtatttc
     3121 ttgggatgag gccatgacca cgatcgtgtc gaaattcgac gagctgcaag cgcgctacgg
     3181 aggcgaatcg ctgttcggca tgtgcggcac gtcgcgcgtg tggtgcatgt tcggagcgtc
     3241 caacggcatg tacctgtggg attcgccgaa catcgtgcag gcgtggcaga tctgcaaggg
     3301 cccgcgccat ttcgccactc ttatgtgctc gagcttcgcc gacagctgga tggagacggt
     3361 tgcccatcct gatgtgtacg tggcctgggg cggtgcatcc gagatgtcga actacgatga
     3421 cagctgccgc accactgtgg atgtggcgac gcgctcgacc acgcacattt gcgtcgatcc
     3481 gcgcgaaacg aacatgggta aagaggcgga cttccagctg cacctgcgac ccggtaccga
     3541 tggggcgatg gcgttggctt ggacgaacgt catcattgaa aacaatctgt acgacgagtt
     3601 gttcacgaag aagtggacca acggtccgtt tctcgtgtgc gaagacatgg agccgaccgg
     3661 ctggaccgag gcttcgccca ttgtcatgac cccattcgaa ctcaagacgc atttgctgaa
     3721 ggaatcggat ctgctggagg acggaagccc aaagcgtttt atggtttggg acaaccttgc
     3781 tgggaggctg acctatttcg attccgaaac cggtttttgg gaaggtgagg aatggacgaa
     3841 gccgacggcc gggaaggaag cttcgcagga gaatctcgcg cctggcttcc atcaaggttt
     3901 tgtcattgat cctactccgt tcgaccctga aatagatccg gcgttgtttg gggaattcga
     3961 agtgacgctc aaagacgggt cgacgcataa ggtgcagccg gtgtttcaaa agtacgccga
     4021 tcgctgcgcg gaatacactc ctgaagtcgc tgaggaaatc accggggttc cggccgatca
     4081 gattcgtgcg gcggctatgg cttacggcac gccccttcat ccggaaaagg gttatggcaa
     4141 tggcggtatt cagtacatgc tggccaccga gcatgcgaat accgccatcc agaatgtgcg
     4201 cgcgcttgat tatctgacgg ccatcacggg aaattacgat acgccgggcg gtcagcgtgc
     4261 gtcaacgcgc gctcccatcg agggcggtca gatgggtttt gccaacaacg gttcgggcat
     4321 gcccatgctt tcgccggggc agatggagaa gatcttaggt cgcgatgaga ttccgctgct
     4381 gtattggtgg ggcatgtggg ccgatgctgc ggctacgtgg aatgcggtgc tcaccggaga
     4441 accgtatccg gtcgttggcg cgttcaattc gtcggggaac ttcatgaacc agtgcaacac
     4501 caccacgaca tgggaggctc tcaagaagct cgatttttat gtggaatgca acctgtggca
     4561 cactcctggc ggtggtttgt gcgacatcgt tttgcctgcc gctcatttct tggagttgtc
     4621 cagtccgcgt tcatcccagg gcgcttcggg cgctatgggc gcgacggtca aatgcatcga
     4681 cccgcctggt gaagcgaagt tcgacggcga gatcattcgc atgttgtacg aatacaaagg
     4741 cctcccctat aacgttattc ctggttatcc tgaatatcct agcgtcgagg aaatgctgga
     4801 catgtcggtg gcgggtttcg agccgaaggg atggaaggta ttcgagcagc gtttccagga
     4861 acatggctgg tgggattgca aggaaactga accggaattg tggggaacgt atcgccgcta
     4921 cgaaaccggt gcgaatcgcg ctcgcaatgc gggtgggttc gtaggttctc aaggtgattg
     4981 gcttcccggg ttctacactc cgacaaagaa gatcgaaata tggtctacgg tcatggagtc
     5041 gttccatccc gatggcgagt ggaatcttcc cacgtatcgc gaaccaccgc acagtcctgt
     5101 gagcgatcct gacatgtaca aagaataccc gctgatcatc actacgggac gccgcattcc
     5161 cgtgtacttc cattccgagc accggcagct gccgtggtgt cgcgagcaat ggccggtgcc
     5221 gcgcgtggaa atcaatccag acgatgccgc cacgtatggc atagaacagg gagattgggt
     5281 ttggattgag acgccatatg ggaagattcg cgaggttgcc gatttgtact acgggatccg
     5341 tcccggcact atcaattgcg agcatcagtg gtggttcccg gaattcaagc aatcgggatc
     5401 gggccacgag ctatgcgcgg tcaaccactt gatagacaac tgggcgcgcg atccgcattg
     5461 cggcgcgtct aacctgcgtg cgtacctcgg caagatatac aaggcaacgc ccgagaactc
     5521 cccgttcggc aatccggtgc cttgcgacca caacggcgtt gagatcatcc acgatgcttc
     5581 cgatcctcgt ctcaaagaat ggctgccgac gtacgaaggg agggagtagg ccatgaccca
     5641 gtatgctatt gtcaccgact tgaatcggtg cgtcgggtgc ttggcatgcg ctgtcgcatg
     5701 caaagccgtg aacgatgtgc ccattggcag tttttggaac aaggttttac gcatcgggcc
     5761 gaatgcttct atacaaggtg cgcaggatgc ggattcttat ctttacttcc tgcccgtgca
     5821 gtgccagcac tgcgccgacc ccgagtgcgt gaaggtgtgc ccgacgggcg cctcgcacaa
     5881 gcttgaggac ggcacggtgc aggttgacaa gtccaagtgc atcggctgcc agttctgcgc
     5941 gatggcttgt ccctacaacg ttcgttactt gaacgagcag gagcgcgtgg ttgagaagtg
     6001 caccctttgc gagcagaaga tcgcccaggg cgagctgccg caatgcgtca gccaatgcgg
     6061 tggcatggcg cgcttcttcg gtgatttgga taagggcatc gaatcgttcg aaggtgcgga
     6121 aaacggaacc gagcgcattg ttctcgggga gttctgcaag cccttcacgg atgaagacgt
     6181 gcacgtgctg cctaatgtgg gaaacaatcc gtcggtgcgc tatatcctgc gtgaccatga
     6241 atggcaagga ggcggtgaat aatggagctc cagtggcctt tgatcgtgtt caccacgctg
     6301 gtggcgtgga gcgcggggct gttcgggacg caggcgctga tggccgcgtt cggcgtcggg
     6361 aagaaggcgc aggtcccggc ctggatcgcg agcgccgtcc ttctggccgt cggcgggatc
     6421 gcggtgttct tccacctcga gcactgggag cgcatcttca acggcttcgg ccacctgacg
     6481 agcggcatca cccaggagct gatcgccatc gtcgtgctgg cggccgtcgc cgtggcctac
     6541 ctggtcatgc tgcgcaagtc cgacgacggc gcgagcgtgc cgaagtggct ggcgtggctg
     6601 tcggtcgcgc tgtcggtcgc gctggtggcc gtgatggcgc actcgtacac gatggccgcg
     6661 aggccggcgt gggatagcgt gctgtggatc ctctacgtcc tgggcaacgc ctgcgtgctg
     6721 ggaccggcga cgttcgcgtt cctcatggcc gcagcgccgc ccgcaggtca gcccgccggg
     6781 cgcgcggccg acgccggggc tcccgcaggc ctcgctgccc tcgtaggcgc cgccgcgaac
     6841 gccctggccg ccctcgcctt cgcgatcttc ctccagctct ccgccggctc cttcgccgac
     6901 gtcggctact acttcgaccc gacgcatccg accgaggcca tggccgacgc cgcggcgacg
     6961 gtcggcgcgc aagcgcccct gctgtggctc ggcgcggtcg ccgtcggcgc tatcgtcccg
     7021 ctcgcggcgg ccttcatggg caggcgctcc ggcaactgga ggctctgggc cccggtggct
     7081 atcatcgccg ccctggcggg cgccgtctgc atgcgcgtgg ccttctacaa cctcggcctc
     7141 agcgtgttca tgttctacta gactagtccg ctggttgcca cgacgccctc gaagtctcct
     7201 tcgagggcgt tctgtcgttt gcaagaaggg tctggagagg caaatcgcgc tcgaaggggc
     7261 ttcgaggccg gggtaggtcg ggagggtgga atccggatgt ttcacgtgaa acatccgttc
     7321 ttttacggcc ggtgtttcgc gtgaaacatt ttgcggaaag gactccttcc cttcccgtgc
     7381 gccaccccaa cgccccgctc ctagcgctcg tacacctggt cgcagttgag cagatccttc
     7441 atggagtgcc agatctggtg cgggttgccc ggcgggttct tgagcggctt catcggatag
     7501 cgctcctgcg cgcccgacag cagatcgacg gcgaacactt cgtcggacag ctcgagcgcg
     7561 ccttccgggc agatgtcggt gcagcagcgg cacttcacgc aatcgaccgg acgatgcgtc
     7621 acgccgatcg agccgtcctc acccgcgtac ttcgcgatgg ccccggtggg gcagaacgtg
     7681 gcgcacatct ggcacgacga gcacttctcg ggatcgatga tcacatggcc ccacaagcgc
     7741 gtgtccatga gcacatcctg cggctgcccc aacgaatcga gcgcggccag caaacgttcg
     7801 cggcggtcgg gaatgaagtg aggcagcgtg ccgtccttgc ccaccttcac gaacctcggc
     7861 tcccgccgct cctccacacc caatgcgtcc ttgacggcgt agtcagccgt caccgccgca
     7921 acgttcttag cctcgtcctt catgctggag aagaagctcc gacggctcgc gtcgtagccc
     7981 tcgtcgcccg cccggcgcac ggatgaggga agcttttccg ccaggtcgat gcgcatatcg
     8041 ttgttccagg ttgcaagcag cgcgcttgcc gtgtcgcaca caacccgcgc catctcgaaa
     8101 cccgagacat gctcgcattg cgcgcagcgc gcctgcacga ggctcacgcg gcgcacgccc
     8161 aacgccgcca gcgtgaccag caggctctcc tccacgcgcc ccaggcacgt gacgcccacc
     8221 accttctcgg ggtcgtacaa cccgtcggcc gcggcaagca gctgatcgca ggcgataacc
     8281 acctgtccgt cggccacgtc agccgccgcg cggcatgctt gcagaagctc ggcatcgttc
     8341 ggacgatgcg cttcaagcgc gcaggtggga cacacggtgg cgcaggttcc gcacccgatg
     8401 caccgctcgg gttcgatgac cagctcgttg tcgtcatagg agatgcaacc gctcgtgcac
     8461 acgtcggcgc acttcatgca cgttgcgttg cgattgcgca caatagcgca acgaccctga
     8521 tgaaccgtaa tgtcggcgct ctgaagttgt tcgagcagac cgaggaaatt cgtcacgtga
     8581 gccatgactt caccctttct cgtctacccc cttgcaccgc acagtataac cactttcgcc
     8641 gtctcacgcg agaaaaaacg cgcttgacac cttcgacagg cctccctatc atgtggcctt
     8701 ggggagtccg ccggcacgct gatcgcgtgc gaagcggaca ttgtttgcaa aagtgggaac
     8761 ctcaggggtg aggtttcgcg gtgctgtgcg ccgtaagtgc aaacaatgtc tgacgagggg
     8821 cggaacatga ctcacgattc cacgaaccag catctttctc ttgagcccga ctacgacggg
     8881 ttcgatccac ttgaagattt cgaagacgat gcgaccgagg tcgaaggccg tgccgacgag
     8941 ttcgtcgcca cggtgagcgc cgccgaagac gcgctcgctc acgacgcgat ggcacccgcc
     9001 acgcccgcca tctgcgaaaa gcccgctgaa gagcgcatcg ccgacttgtt ggaggccatg
     9061 gcgccgcgcc gcacggtgct gctgggcatc ctggccgcct gcgccgagcc gcagccggca
     9121 tccgccgtga acgctcgcgt cgacgagctg caggagaaca acttctcggt gtacagcgcg
     9181 gccaacctgt gctccttgct ggagaaggcg ggcgccatcg agcgcgtcac ggccgagggc
     9241 gaacccgcgg aggacatcga aatcgagcca cagaccgtcg tcgtcgacgg cgtcgaatac
     9301 ctggaagccc gcgaacccgt cgagatctac tggcacatca ccgagccggg ccgcgcggcg
     9361 cttgaggccg acaaaccgct cgagcgcctg cgcgccctcc tggacgaaga tgccgcctac
     9421 gcgcccatct accagcgcat cctccgcttg tgcacagcag acggcggcgc cacgacaccc
     9481 gccatcaaca acgcggtcga ccacaacccg ctggtgcaga agccgcgctt ctacgcgccc
     9541 cacttcgtcg accgcctgga gaaatgcgac gcgctggcct ggaagaaggc gtggtgcatc
     9601 accgacatcg gacgcgccgg cctcgatatg ctggcagacg tcgtagacga gaacgcgccc
     9661 gccacccagc ccgaaacccc cgcaacccct gatcccgccg catccaagga ggactaacca
     9721 tgaccgaagc aaccgcgccc gcaaccgaga ccctcgccga cgaagcgacc atgcaggagc
     9781 tcgtcaaact gaacgagcag cgcgccgcca cgtacggcct gctgtcgcgc ctgtaccgcg
     9841 tggaggtgga ccagcccctt ctcgacgagc tgcgcagcat gcgcttcccc gccaagacgg
     9901 gcaacgagaa cgtggacgag ggctaccgcc tgctggccac gtacctgagc aacacctggg
     9961 acaacagcgt caccgacctc gccatcgact acgtgcgcgt gttcatcgga cacggcatcg
    10021 acgcgttctc ggccgcctac ccgttcgaat cggtgtacac ctccgagaag cgcctgctca
    10081 tgcaggaagc acgcgacgag gtgctggcca tctaccgcag cgcaggcctc gacaagcagg
    10141 acacatggaa agagggcgag gatcatatcg cgctcgagct ggaattcgag cagatcatgg
    10201 ccacgcgcac ggtcgaagcg ctgcgcaaag gcgacgaaga cgaggccgtc gcgctgctga
    10261 ccacgcagaa gaactttctc gaagaccatc tggcggcatg ggctcccatg atgacctccg
    10321 acatgaagcg attcgcgcaa accgacttct accggggctt ggcgtacttg acggacgggt
    10381 tcctccaaac cgaccaaacg ttcctcgacg acgtgctggc cgaagactaa ctgcacagga
    10441 tcgaaagaag gagcgatgga caacaagcag gagaaaacct acgccggcat gacgcgacgc
    10501 acgctgtgcc tgggcgcagg cggcgctgcg gtcatgctag gtctaggcgg attgaagtac
    10561 gcgggatcgc aaccgctcgt gcgcccgccg ggaggccagg acgaggaacg gctcatctcg
    10621 gcctgcatcc gctgcgagaa gtgctacgag atctgcccgc gcgacgtcat cgcgcccgcc
    10681 cacctcgagg acggtgtgct gaacatgcgc acccccacgt tcgacttctc ggccaactgg
    10741 tgcgactggt gcgccgaaga gcacgacggc accccgttgt gcgtgagcgc ctgccccacc
    10801 gaagcgctca agctgcccga cgacgccacg gccgagaaca ccatcatcgg caaagccgtt
    10861 atcaacacgg actggtgctt ggcgtacaag ctgatcggat gccggttttg ctacgacgcc
    10921 tgtccctacg aggccatgga gctcgacgag aacaaccgcc ccgtcgtgat cgaggacaag
    10981 tgcaacggct gcggcgcgtg cgagagcgtg tgcgtctcgc tgcagaacgg ctccatctcc
    11041 gaaggagcca ccgcgcgcgc catcgtcgtc cacccgcttg acgaagttga aggataaggt
    11101 gccctcatga agaagaactc caagaagctg cgcaccctcg tcgcactggc catcgtggcc
    11161 gtcgtcgccg tcggcttcgt cacgaacgtc gggatcggca ccatgtcggc accgggcatc
    11221 tgggacatct ccatcctatg cccgctgggc gccttgggaa cgatgctcgc ctccaagatg
    11281 atggtgccgc gcgccgtaat ctcgctgatc gtgatggtgc tgctcatcgt tctgttcgcc
    11341 cgcgcgttct gcggatggat ttgccccgtg ccgctcgtgc agaagttgcg cggcttgttc
    11401 gcgaagaagc aacccgtcga acgcggcgac gatgccgatc ctgccgcggc gccgctcacg
    11461 aaagtcgaga tcgacgcgct gggcgtttca tgctcgaagg ggtcgaagaa tggcagcgga
    11521 tgcgcgtcct gcgcctccaa gcgcggagcc gcgctcgatg ctcgccactt cgtgctgggc
    11581 ggcgcgctgg cgtccacctt catctttgga ttcccggtgt tctgcctcgt ctgccccatc
    11641 ggactcacgt tcgccaccat cctgctggtc atcaacctgt tctcacacgg cgatgtcacc
    11701 tggtcgctca tcgtggtgcc cgcgctgctt atcgccgagg tgctgctgtt caagaagtgg
    11761 tgccataagc tgtgcccgct ttcggcgttc atgagcctga tcgcgaaggc gaacaagacc
    11821 ttcgtgccca cgatcgacga tgcgaagtgc ctggaaacgg cgaagggcgg cgcgtgcggg
    11881 gcctgcggcc gcgtctgcga ggagggcatc gacccgcgcc acccgcagct ttcgcaggcg
    11941 gcatggagcg agtgcaccaa gtgccgcgcc tgcgtggacg catgccctgc gaacgcgatc
    12001 accatgccgc tgctgccgcg gcgaacgccg tctaccgcag aacctgtcgc cgtgccaacg
    12061 aacgccgaga agatcgacgc ggagaatgcg cgccccgttc cgtaaagccc ctcctgcgcg
    12121 acccggctcg cagctgcagc ggtctggtcg cgcatccgga atcgggccga gcccgacccc
    12181 gggcaaaagg cggcttggca agggctttgg gggacgagcc gccttttgcc cgggagcgac
    12241 atccccgctc cccctccctg cgaaaagagg gatgccgctt ccggccttca tctgacaacc
    12301 acgacgtggg aggacgaaaa cccatggcat acgaaacgac aagcgaatcg accgaggccc
    12361 tgctcgtcac cggaggcgtc ggcaccggca aaacgcaacg tctcgtcgag cgcgctgccg
    12421 cgctgctggc cgaaaacgcc gccgcgggca acgtgctcgt gctgtgcgcg acgccccagg
    12481 ccgcacgcct gttctcggag cggttgacag cccgcgtcgg tgccaagcgc gctgccggcg
    12541 caaccgtcgc caccgcacgc gccttagcgc tcgacgtgct ggccgacgac ggcgcgcgcc
    12601 gctggtcggg gcgcgagccg cgactactga ccgccttcga agagacgttc ctcatggagg
    12661 acatgaaggt gagcgggctg cggccgaagc ggctgcgcga gatgctcaag ttcttctacc
    12721 gctcgtggac cgagctggcg gacgacgatc ccgattggct gctaccgggc gaggaggccg
    12781 atgtccatac gctgttgaaa gagaacctgg tcttcatgcg cgccgtcata gaacccgaag
    12841 cggcgaacct tgctgcacgc tacctccgag aacacgacga agcgcgggcg gcgcattcct
    12901 acgcgcacgt gctgatggac gactacgcct gcgcaagccg tgcctcgcag acgctggcga
    12961 atctcgtcgc aacctgctcg atcaccgtgg cgggcgacgc ggaaacctgc atcgagacat
    13021 acgattcgta cccctatgcc gccggaatcg acgaattcct cgcaacgcat cccgatgctg
    13081 cttgcgagca cctggatgcc gacgcgcccg atgccgtcga acgggtcgct ctggcaagcg
    13141 acacgcccga agccgagttc gaacaggtgg cgcgcacggt ggccgaagcc gtcgccgcag
    13201 gaacgcccgc acggcagatc gtcgtggccg taccgaacag cgtgtggtcg cgcaacatcg
    13261 caaccgcttt gcacgcacga ggcgtgccaa ccgaagcgct cgccatctcg cagcccctgc
    13321 gcggcgacgt gcgggacaac gcccgctgca cgcctgcgcg catcgccacg gccctcaacc
    13381 tcgtggcgaa cccagacgac accgcggctt ggcggtgctg gtgtggattc ggcgactacc
    13441 tggtgaacag cgcggctttc gccagcctgc ggacttgcgc gaaggagcgc agtatcggcc
    13501 tcgtggacgc tttggagatg ctaagcgaaa acgactgcga gcacgtagtg ggcgcgcaac
    13561 gcgtggcgac cgcctacaag gacggtcgcg cgctcatcga gcaggtacga ggattgcggg
    13621 gcacggcact gctggacgag ctgacgcgca tcgtcaccga cggagcggaa tcccaagcgc
    13681 ccgccgttct gcgcgagctc tgcctcgagc acggcaacgc agccgatgaa aacccggaaa
    13741 gcctttacga tgccgatgct gcggcgatgg cgcggcgcat ggccgagcgc ctgctcgcgc
    13801 ccacgctgga ggttgccgac gcggtagccg tcgtccccta cgacttcgcc tgcggcttct
    13861 cgcccgacct gctgatcgtc gccggcttcg tgaacggttt catcccctcc cgcgactatt
    13921 tcgacgccac ggtgaccccg cccgagcgcc aggagaagat gcgcgccgtc gacgaacgac
    13981 gcgtgcgcgc gctcacagga aaggccggcc gcgcggtggc gctgtcgtac ttcacgtcca
    14041 ccgacctcga aagcgccggc aagctgaagc tcaagatcag tcgcatccgc ctcaagaacg
    14101 gtatccgcac ctgcactata gagcccagcc tattcctgga gcaaatcgca gcgcaggagt
    14161 agcgactcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagagaga
    14221 atataaaaag ccagattatt aatccggctt ttttattatt taattcctgt tataaaaaaa
    14281 ggatcaattt tgaactctct cccaaagttg atcccttaac gatccctgca ggagaaggcc
    14341 atcctgacgg atggcctttt tgaagctttc tagaatcggg ggcccccggg gggattctag
    14401 aacaatggaa gtcctcgtct cgccctcgaa ttagcccgct taatgagcgg gctttttttg
    14461 aattaattct cgcgagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
    14521 acgcaattaa tgtaagttag cgcgaattgc aagctggccg acgcgctggg ctacgtcttg
    14581 ctggcgttcg ggagcagaag agcatacatc tggaagcaaa gccaggaaag cggcctatgg
    14641 agctgtgcgg cagcgctcag taggcaattt ttcaaaatat tgttaagcct tttctgagca
    14701 tggtattttt catggtatta ccaattagca ggaaaataag ccattgaata taaaagataa
    14761 aaatgtcttg tttacaatag agtggggggg gtcagcctgc cgccttgggc cgggtgatgt
    14821 cgtacttgcc cgccgcgaac tcggttaccg tccagcccag cgcgaccagc tccggcaacg
    14881 cctcgcgcac ccgctggcgg cgcttgcgca tggtcgaacc actggcctct gacggccaga
    14941 catagccgca caaggtatct atggaagcct tgccggtttt gccggggtcg atccagccac
    15001 acagccgctg gtgcagcagg cgggcggttt cgctgtccag cgcccgcacc tcgtccatgc
    15061 tgatgcgcac atgctggccg ccacccatga cggcctgcgc gatcaagggg ttcagggcca
    15121 cgtacaggcg cccgtccgcc tcgtcgctgg cgtactccga cagcagccga aacccctgcc
    15181 gcttgcggcc attctgggcg atgatggata ccttccaaag gcgctcgatg cagtcctgta
    15241 tgtgcttgag cgccccacca ctatcgacct ctgccccgat ttcctttgcc agcgcccgat
    15301 agctaccttt gaccaccatg gcatcagcgg tgacggcctc ccacttgggt tccaggaaca
    15361 gccggagctg ccgtccgcct tcggtcttgg gttccgggcc aagcactagg ccattaggcc
    15421 cagccatggc caccagccct tgcaggatgc gcagatcatc agcgcccagc ggctccgggc
    15481 cgctgaactc gatccgcttg ccgtcgccgt agtcatacgt cacgtccagc ttgctgcgct
    15541 tgcgctcgcc ccgcttgagg gcacggaaca ggccgggggc cagacagtgc gccgggtcgt
    15601 gtcggacgtg gctgaggctg tgcttgttct taggcttcac cacggggcac ccccttgctc
    15661 ttgcgctgcc tctccagcac ggcgggcttg agcaccccgc cgtcatgccg cctgaaccac
    15721 cgatcagcga acggtgcgcc atagttggcc ttgctcacac cgaagcggac gaagaaccgg
    15781 cgctggtcgt cgtccacacc ccattcctcg gcctcggcgc tggtcatgct cgacaggtag
    15841 gactgccagc ggatgttatc gaccagtacc gagctgcccc ggctggcctg ctgctggtcg
    15901 cctgcgccca tcatggccgc gcccttgctg gcatggtgca ggaacacgat agagcacccg
    15961 gtatcggcgg cgatggcctc catgcgaccg atgacctggg ccatggggcc gctggcgttt
    16021 tcttcctcga tgtggaaccg gcgcagcgtg tccagcacca tcaggcggcg gccctcggcg
    16081 gcgcgcttga ggccgtcgaa ccactccggg gccatgatgt tgggcaggct gccgatcagc
    16141 ggctggatca gcaggccgtc agccacggct tgccgttcct cggcgctgag gtgcgcccca
    16201 agggcgtgca ggcggtgatg aatggcggtg ggcgggtctt cggcgggcag gtagatcacc
    16261 gggccggtgg gcagttcgcc cacctccagc agatccggcc cgcctgcaat ctgtgcggcc
    16321 agttgcaggg ccagcatgga tttaccggca ccaccgggcg acaccagcgc cccgaccgta
    16381 ccggccacca tgttgggcaa aacgtagtcc agcggtggcg gcgctgctgc gaacgcctcc
    16441 agaatattga taggcttatg ggtagccatt gattgcctcc tttgcaggca gttggtggtt
    16501 aggcgctggc ggggtcacta cccccgccct gcgccgctct gagttcttcc aggcactcgc
    16561 gcagcgcctc gtattcgtcg tcggtcagcc agaacttgcg ctgacgcatc cctttggcct
    16621 tcatgcgctc ggcatatcgc gcttggcgta cagcgtcagg gctggccagc aggtcgccgg
    16681 tctgcttgtc cttttggtct ttcatatcag tcaccgagaa acttgccggg gccgaaaggc
    16741 ttgtcttcgc ggaacaagga caaggtgcag ccgtcaaggt taaggctggc catatcagcg
    16801 actgaaaagc ggccagcctc ggccttgttt gacgtataac caaagccacc gggcaaccaa
    16861 tagcccttgt cacttttgat caggtagacc gaccctgaag cgcttttttc gtattccata
    16921 aaaccccctt ctgtgcgtga gtactcatag tataacaggc gtgagtacca acgcaagcac
    16981 tacatgctga aatctggccc gcccctgtcc atgcctcgct ggcggggtgc cggtgcccgt
    17041 gccagctcgg cccgcgcaag ctggacgctg ggcagaccca tgaccttgct gacggtgcgc
    17101 tcgatgtaat ccgcttcgtg gccgggcttg cgctctgcca gcgctgggct ggcctcggcc
    17161 atggccttgc cgatttcctc ggcactgcgg ccccggctgg ccagcttctg cgcggcgata
    17221 aagtcgcact tgctgaggtc atcaccgaag cgcttgacca gcccggccat ctcgctgcgg
    17281 tactcgtcca gcgccgtgcg ccggtggcgg ctaagctgcc gctcggacag ttcgaggctg
    17341 gccagcctgc gggccttctc ctgctgccgc tgggcctgct cgatctgctg gccagcctgc
    17401 tgcaccagcg ccgggccagc ggtggcggtc ttgcccttgg attcacgcag cagcacccac
    17461 ggctgataac cggcgcgggt ggtgtgcttg tccttgcggt tggtgaagcc cgccaagcgg
    17521 ccatagtggc ggctgtcggc gctggccggg tcggcgtcgt actcgctggc cagcgtccgg
    17581 gcaatctgcc cccgaagttc accgcctgcg gcgtcggcca ccttgaccca tgcctgatag
    17641 ttcttcgggc tggtttccac taccagggca ggctcccggc cctcggcttt catgtcatcc
    17701 aggtcaaact cgctgaggtc gtccaccagc accagaccat gccgctcctg ctcggcgggc
    17761 ctgatataca cgtcattgcc ctgggcattc atccgcttga gccatggcgt gttctggagc
    17821 acttcggcgg ctgaccattc ccggttcatc atctggccgg tggtggcgtc cctgacgccg
    17881 atatcgaagc gctcacagcc catggccttg agctgtcggc ctatggcctg caaagtcctg
    17941 tcgttcttca tcgggccacc aagcgcagcc agatcgagcc gtcctcggtt gtcagtggcg
    18001 tcaggtcgag caagagcaac gatgcgatca gcagcaccac cgtaggcatc atggaagcca
    18061 gcatcacggt tagccatagc ttccagtgcc acccccgcga cgcgctccgg gcgctctgcg
    18121 cggcgctgct cacctcggcg gctacctccc gcaactcttt ggccagctcc acccatgccg
    18181 cccctgtctg gcgctgggct ttcagccact ccgccgcctg cgcctcgctg gcctgctggg
    18241 tctggctcat gacctgccgg gcttcgtcgg ccagtgtcgc catgctctgg gccagcggtt
    18301 cgatctgctc cgctaactcg ttgatgcctc tggatttctt cactctgtcg attgcgttca
    18361 tggtctattg cctcccggta ttcctgtaag tcgatgatct gggcgttggc ggtgtcgatg
    18421 ttcagggcca cgtctgcccg gtcggtgcgg atgccccggc cttccatctc caccacgttc
    18481 ggccccaggt gaacaccggg caggcgctcg atgccctgcg cctcaagtgt tctgtggtca
    18541 atgcgggcgt cgtggcctgc ccgctctaat gcccggttgg catggtcggc ccatgcctcg
    18601 cgggtctgct caagccatgc cttgggcttg agcgcttcgg tcttctgtgc cccgcccttc
    18661 tccggggtct tgccgttgta ccgcttgaac cactgagcgg cgggccgctc gatgccgtca
    18721 ttgatccgct cggagatcat caggtggcag tgcgggttct cgccgccacc ggcatggatg
    18781 gccagcgtat acggcaggcg ctcggcaccg gtcaggtgct gggcgaactc ggacgccagc
    18841 gccttctgct ggtcgagggt cagctcgacc ggcagggcaa attcgacctc cttgaacagc
    18901 cgcccattgg cgcgttcata caggtcggca gcatcccagt agtcggcggg ccgctcgacg
    18961 aactccggca tgtgcccgga ttcggcgtgc aagacttcat ccatgtcgcg ggcatacttg
    19021 ccttcgcgct ggatgaagtc ggccttggcc ctggccgatt ggccgcccga cctgctgccg
    19081 gttttcgccg taaggtgata aatcgccatg ctgcctcgct gttgcttttg cttttcggct
    19141 ccatgcaatg gccctcggag agcgcaccgc ccgaagggtg gccgttaggc cagtttctcg
    19201 aagagaaacc ggtaagtgcg ccctccccta caaagtaggg tcgggattgc cgccgctgtg
    19261 cctccatgat agcctacgag acagcacatt aacaatgggg tgtcaagatg gttaagggga
    19321 gcaacaaggc ggcggatcgg ctggccaagc tcgaagaaca acgagcgcga atcaatgccg
    19381 aaattcagcg ggtgcgggca agggaacagc agcaagagcg caagaacgaa acaaggcgca
    19441 aggtgctggt gggggccatg attttggcca aggtgaacag cagcgagtgg ccggaggatc
    19501 ggctcatggc ggcaatggat gcgtaccttg aacgcgacca cgaccgcgcc ttgttcggtc
    19561 tgccgccacg ccagaaggat gagccgggct gaatgatcga ccgagacagg ccctgcgggg
    19621 ctgcacacgc gcccccaccc ttcgggtagg gggaaaggcc gctaaagcgg ctaaaagcgc
    19681 tccagcgtat ttctgcgggg tttggtgtgg ggtttagcgg gctttgcccg cctttccccc
    19741 tgccgcgcag cggtggggcg gtgtgtagcc tagcgcagcg aatagaccag ctatccggcc
    19801 tctggccggg catattgggc aagggcagca gcgccccaca agggcgctga taaccgcgcc
    19861 tagtggatta ttcttagata atcatggatg gatttttcca acaccccgcc agcccccgcc
    19921 cctgctgggt ttgcaggttt gggggcgtga cagttattgc aggggttcgt gacagttatt
    19981 gcaggggggc gtgacagtta ttgcaggggt tcgtgacagt tagtacggga gtgacgggca
    20041 ctggctggca atgtctagca acggcaggca tttcggctga gggtaaaaga actttccgct
    20101 aagcgataga ctgtatgtaa acacagtatt gcaaggacgc ggaacatgcc tcatgtggcg
    20161 gccctcgcga gaattaattc agataaaaaa aatccttagc tttcgctaag gatgatttct
    20221 agcgatgacc ctgctgattg gttcgctgac cgtagactgg ttggcttggt ttcatcagcc
    20281 atccgcttgc cctcatctgt tacgccggcg gtagccggcc agcctcgcag agcaggattc
    20341 ccgttgagca ccgccaggtg cgaataaggg acagtgaaga aggaacaccc gctcgcgggt
    20401 gggcctactt cacctatcct gcccggctga cgccgttgga tacaccaagg aaagtctaca
    20461 cgaacccttt ggcaaaatcc tgtatatcgt gcgacatcgc gcgcgcgcgc gcggatgcta
    20521 gcggcgcgcc aaaaaaaagc ccgctcatta
//
