LOCUS       sequence-466023-       13674 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SED1 biosensor for expression in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13674)
  AUTHORS   Cuevas-Velazquez CL, Vellosillo T, Guadalupe K, Schmidt HB, Yu F, 
            Moses D, Brophy JAN, Cosio-Acosta D, Das A, Wang L, Jones AM, 
            Covarrubias AA, Sukenik S, Dinneny JR
  TITLE     Intrinsically disordered protein biosensor tracks the 
            physical-chemical effects of osmotic stress on cells.
  JOURNAL   Nat Commun. 2021 Sep 14;12(1):5438. doi: 10.1038/s41467-021-25736-8.
  PUBMED    34521831
REFERENCE   2  (bases 1 to 13674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-09-14"; volume: "12(1):5438. doi"; pages: " 
            10.1038/s41467-021-25736-8"
COMMENT     Sequence Label: pGPTVII-SED1
FEATURES             Location/Qualifiers
     source          1..13674
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  1317..2084
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             2276..3070
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             3369..4517
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    6113..6137
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(6384..6405)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6596..6615
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6740..6762
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6754..6771
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6755..6771
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(6784..6984)
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     CDS             complement(7019..7570)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(7621..7800)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             10359..10370
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     misc_feature    11197..11221
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             11686..12336
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     primer_bind     complement(12524..12541)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(12775..12794)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            13017..13126
                     /note="incP origin of transfer"
     CDS             13159..13530
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      join(13667..13674,1..624)
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 ccgggctggt tgccctcgcc gctgggctgg cggccgtcta tggccctgca aacgcgccag
       61 aaacgccgtc gaagccgtgt gcgagacacc gcggccgccg gcgttgtgga tacctcgcgg
      121 aaaacttggc cctcactgac agatgagggg cggacgttga cacttgaggg gccgactcac
      181 ccggcgcggc gttgacagat gaggggcagg ctcgatttcg gccggcgacg tggagctggc
      241 cagcctcgca aatcggcgaa aacgcctgat tttacgcgag tttcccacag atgatgtgga
      301 caagcctggg gataagtgcc ctgcggtatt gacacttgag gggcgcgact actgacagat
      361 gaggggcgcg atccttgaca cttgaggggc agagtgctga cagatgaggg gcgcacctat
      421 tgacatttga ggggctgtcc acaggcagaa aatccagcat ttgcaagggt ttccgcccgt
      481 ttttcggcca ccgctaacct gtcttttaac ctgcttttaa accaatattt ataaaccttg
      541 tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac gccgaagggg ggtgcccccc
      601 cttctcgaac cctcccggcc cgctaacgcg ggcctcccat ccccccaggg gctgcgcccc
      661 tcggccgcga acggcctcac cccaaaaatg gcagcgctgg cagtccttgc cattgccggg
      721 atcggggcag taacgggatg ggcgatcagc ccgagcgcga cgcccggaag cattgacgtg
      781 ccgcaggtgc tggcatcgac attcagcgac caggtgccgg gcagtgaggg cggcggcctg
      841 ggtggcggcc tgcccttcac ttcggccgtc ggggcattca cggacttcat ggcggggccg
      901 gcaattttta ccttgggcat tcttggcata gtggtcgcgg gtgccgtgct cgtgttcggg
      961 ggtgcgataa acccagcgaa ccatttgagg tgataggtaa gattataccg aggtatgaaa
     1021 acgagaattg gacctttaca gaattactct atgaagcgcc atatttaaaa agctaccaag
     1081 acgaagagga tgaagaggat gaggaggcag attgccttga atatattgac aatactgata
     1141 agataatata tcttttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc
     1201 ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga
     1261 ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca taattgggta
     1321 atgactccaa cttattgata gtgttttatg ttcagataat gcccgatgac tttgtcatgc
     1381 agctccaccg attttgagaa cgacagcgac ttccgtccca gccgtgccag gtgctgcctc
     1441 agattcaggt tatgccgctc aattcgctgc gtatatcgct tgctgattac gtgcagcttt
     1501 cccttcaggc gggattcata cagcggccag ccatccgtca tccatatcac cacgtcaaag
     1561 ggtgacagca ggctcataag acgccccagc gtcgccatag tgcgttcacc gaatacgtgc
     1621 gcaacaaccg tcttccggag actgtcatac gcgtaaaaca gccagcgctg gcgcgattta
     1681 gccccgacat agccccactg ttcgtccatt tccgcgcaga cgatgacgtc actgcccggc
     1741 tgtatgcgcg aggttaccga ctgcggcctg agttttttaa gtgacgtaaa atcgtgttga
     1801 ggccaacgcc cataatgcgg gctgttgccc ggcatccaac gccattcatg gccatatcaa
     1861 tgattttctg gtgcgtaccg ggttgagaag cggtgtaagt gaactgcagt tgccatgttt
     1921 tacggcagtg agagcagaga tagcgctgat gtccggcggt gcttttgccg ttacgcacca
     1981 ccccgtcagt agctgaacag gagggacagc tgatagaaac agaagccact ggagcacctc
     2041 aaaaacacca tcatacacta aatcagtaag ttggcagcat cacccataat tgtggtttca
     2101 aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt gaaaaagctg
     2161 ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc gtcttgttat
     2221 aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat aataaatggc
     2281 taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga
     2341 tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg aaaacctata
     2401 tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac gggaaaagga
     2461 catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact ttgaacggca
     2521 tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct cggaagagta
     2581 tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt
     2641 tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc gcttagccga
     2701 attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact gggaagaaga
     2761 cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa agcccgaaga
     2821 ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga aagatggcaa
     2881 agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt atgacattgc
     2941 cttctgcgtc cggtcgatca gggaggatat cggggaagaa cagtatgtcg agctattttt
     3001 tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga
     3061 attgttttag tacctagatg tggcgcaacg atgccggcga caagcaggag cgcaccgact
     3121 tcttccgcat caagtgtttt ggctctcagg ccgaggccca cggcaagtat ttgggcaagg
     3181 ggtcgctggt attcgtgcag ggcaagattc ggaataccaa gtacgagaag gacggccaga
     3241 cggtctacgg gaccgacttc attgccgata aggtggatta tctggacacc aaggcaccag
     3301 gcgggtcaaa tcaggaataa gggcacattg ccccggcgtg agtcggggca atcccgcaag
     3361 gagggtgaat gaatcggacg tttgaccgga aggcatacag gcaagaactg atcgacgcgg
     3421 ggttttccgc cgaggatgcc gaaaccatcg caagccgcac cgtcatgcgt gcgccccgcg
     3481 aaaccttcca gtccgtcggc tcgatggtcc agcaagctac ggccaagatc gagcgcgaca
     3541 gcgtgcaact ggctccccct gccctgcccg cgccatcggc cgccgtggag cgttcgcgtc
     3601 gtctcgaaca ggaggcggca ggtttggcga agtcgatgac catcgacacg cgaggaacta
     3661 tgacgaccaa gaagcgaaaa accgccggcg aggacctggc aaaacaggtc agcgaggcca
     3721 agcaggccgc gttgctgaaa cacacgaagc agcagatcaa ggaaatgcag ctttccttgt
     3781 tcgatattgc gccgtggccg gacacgatgc gagcgatgcc aaacgacacg gcccgctctg
     3841 ccctgttcac cacgcgcaac aagaaaatcc cgcgcgaggc gctgcaaaac aaggtcattt
     3901 tccacgtcaa caaggacgtg aagatcacct acaccggcgt cgagctgcgg gccgacgatg
     3961 acgaactggt gtggcagcag gtgttggagt acgcgaagcg cacccctatc ggcgagccga
     4021 tcaccttcac gttctacgag ctttgccagg acctgggctg gtcgatcaat ggccggtatt
     4081 acacgaaggc cgaggaatgc ctgtcgcgcc tacaggcgac ggcgatgggc ttcacgtccg
     4141 accgcgttgg gcacctggaa tcggtgtcgc tgctgcaccg cttccgcgtc ctggaccgtg
     4201 gcaagaaaac gtcccgttgc caggtcctga tcgacgagga aatcgtcgtg ctgtttgctg
     4261 gcgaccacta cacgaaattc atatgggaga agtaccgcaa gctgtcgccg acggcccgac
     4321 ggatgttcga ctatttcagc tcgcaccggg agccgtaccc gctcaagctg gaaaccttcc
     4381 gcctcatgtg cggatcggat tccacccgcg tgaagaagtg gcgcgagcag gtcggcgaag
     4441 cctgcgaaga gttgcgaggc agcggcctgg tggaacacgc ctgggtcaat gatgacctgg
     4501 tgcattgcaa acgctagggc cttgtggggt cagttccggc tgggggttca gcagccagcg
     4561 ctttactggc atttcaggaa caagcgggca ctgctcgacg cacttgcttc gctcagtatc
     4621 gctcgggacg cacggcgcgc tctacgaact gccgataaac agaggattaa aattgacaat
     4681 tgtgattaag gctcagattc gacggcttgg agcggccgac gtgcaggatt tccgcgagat
     4741 ccgattgtcg gccctgaaga aagctccaga gatgttcggg tccgtttacg agcacgagga
     4801 gaaaaagccc atggaggcgt tcgctgaacg gttgcgagat gccgtggcat tcggcgccta
     4861 catcgacggc gagatcattg ggctgtcggt cttcaaacag gaggacggcc ccaaggacgc
     4921 tcacaaggcg catctgtccg gcgttttcgt ggagcccgaa cagcgaggcc gaggggtcgc
     4981 cggtatgctg ctgcgggcgt tgccggcggg tttattgctc gtgatgatcg tccgacagat
     5041 tccaacggga atctggtgga tgcgcatctt catcctcggc gcacttaata tttcgctatt
     5101 ctggagcttg ttgtttattt cggtctaccg cctgccgggc ggggtcgcgg cgacggtagg
     5161 cgctgtgcag ccgctgatgg tcgtgttcat ctctgccgct ctgctaggta gcccgatacg
     5221 attgatggcg gtcctggggg ctatttgcgg aactgcgggc gtggcgctgt tggtgttgac
     5281 accaaacgca gcgctagatc ctgtcggcgt cgcagcgggc ctggcggggg cggtttccat
     5341 ggcgttcgga accgtgctga cccgcaagtg gcaacctccc gtgcctctgc tcacctttac
     5401 cgcctggcaa ctggcggccg gaggacttct gctcgttcca gtagctttag tgtttgatcc
     5461 gccaatcccg atgcctacag gaaccaatgt tctcggcctg gcgtggctcg gcctgatcgg
     5521 agcgggttta acctacttcc tttggttccg ggggatctcg cgactcgaac ctacagttgt
     5581 ttccttactg ggctttctca gccccagatc tggggtcgat cagccgggga tgcatcaggc
     5641 cgacagtcgg aacttcgggt ccccgacctg taccattcgg tgagcaatgg ataggggagt
     5701 tgatatcgtc aacgttcact tctaaagaaa tagcgccact cagcttcctc agcggcttta
     5761 tccagcgatt tcctattatg tcggcatagt tctcaagatc gacagcctgt cacggttaag
     5821 cgagaaatga ataagaaggc tgataattcg gatctctgcg agggagatga tatttgatca
     5881 caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt
     5941 gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag
     6001 tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat
     6061 cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga
     6121 tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt
     6181 taatgtactg gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc
     6241 accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga
     6301 aaatcctgtt tgatggtggt tccgaaatcg gcaaaatccc ttataaatca aaagaatagc
     6361 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     6421 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     6481 cacccaaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     6541 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     6601 agaaagcgaa aggagcgggc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     6661 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     6721 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattaatt
     6781 cccatcttga aagaaatata gtttaaatat ttattgataa aataacaagt caggtattat
     6841 agtccaagca aaaacataaa tttattgatg caagtttaaa ttcagaaata tttcaataac
     6901 tgattatatc agctggtaca ttgccgtaga tgaaagactg agtgcgatat tatgtgtaat
     6961 acataaattg atgatatagc tagcttagct catcggggga tcgatcctct aggggtcatc
     7021 agatctcggt gacgggcagg accggacggg gcggtaccgg caggctgaag tccagctgcc
     7081 agaaacccac gtcatgccag ttcccgtgct tgaagccggc cgcccgcagc atgccgcggg
     7141 gggcatatcc gagcgcctcg tgcatgcgca cgctcgggtc gttgggcagc ccgatgacag
     7201 cgaccacgct cttgaagccc tgtgcctcca gggacttcag caggtgggtg tagagcgtgg
     7261 agcccagtcc cgtccgctgg tggcgggggg agacgtacac ggtcgactcg gccgtccagt
     7321 cgtaggcgtt gcgtgccttc caggggcccg cgtaggcgat gccggcgacc tcgccgtcca
     7381 cctcggcgac gagccaggga tagcgctccc gcagacggac gaggtcgtcc gtccactcct
     7441 gcggttcctg cggctcggta cggaagttga ccgtgcttgt ctcgatgtag tggttgacga
     7501 tggtgcagac cgccggcatg tccgcctcgg tggcacggcg gatgtcggcc gggcgtcgtt
     7561 ctgggctcat ggtagatccc cctagagtcg agatctggat tgagagtgaa tatgagactc
     7621 taattggata ccgaggggaa tttatggaac gtcagtggag catttttgac aagaaatatt
     7681 tgctagctga tagtgacctt aggcgacttt tgaacgcgca ataatggttt ctgacgtatg
     7741 tgcttagctc attaaactcc agaaacccgc ggctgagtgg ctccttcaac gttgcggttc
     7801 tgtcagttcc aaacgtaaaa cggcttgtcc cgcgtcatcg gcgggggtca taacgtgact
     7861 cccttaattc tccgctcatg atcttgatcc cctgcgccat cagatccttg gcggcaagaa
     7921 agccatccag tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa
     7981 ttccggttcg cttgctgtcc ataaaaccgc ccagtctagc tatcgccatg taagcccact
     8041 gcaagctacc tgctttctct ttgcgcttgc gttttccctt gtccagatag cccagtagct
     8101 gacattcatc cggggtcagc accgtttctg cggactggct ttctacgtgt tccgcttcct
     8161 ttagcagccc ttgcgccctg agtgcttgcg gcagcgtgaa gcttcgacga gtcagtaata
     8221 aacggcgtca aagtggttgc agccggcaca cacgagtcgt gtttatcaac tcaaagcaca
     8281 aatacttttc ctcaacctaa aaataaggca attagccaaa aacaactttg cgtgtaaaca
     8341 acgctcaata cacgtgtcat tttattatta gctattgctt caccgcctta gctttctcgt
     8401 gacctagtcg tcctcgtctt ttcttcttct tcttctataa aacaataccc aaagacctct
     8461 tcttcttcac aattcagatt tcaatttctc aaaatcttaa aaactttctc tcaattctct
     8521 ctaccgtgat caaggtaaat ttctgtgttc cttattctct caaaatcttc gattttgttt
     8581 tcgttcgatc ccaatttcgt atatgttctt tggtttagat tctgttaatc ttagatcgaa
     8641 gacgattttc tgggtttgat cgttagatat catcttaatt ctcgattagg gtttcataga
     8701 tatcatccga tttgttcaaa taatttgagt tttgtcgaat aattactctt cgatttgtga
     8761 tttctatcta gatctggtgt tagtttctag tttgtgcgat cgaatttgtc gattaatctg
     8821 agtttttctg attaacagac tagtatggtg agcaaaggtg aagaactgtt taccggtgtt
     8881 gttccgattc tggttgaact ggatggtgac gttaatggtc acaaattttc agttagcggt
     8941 gaaggcgaag gtgatgcaac ctatggtaaa ctgaccctga aatttatctg taccaccggc
     9001 aaactgccgg ttccgtggcc gaccctggtt accaccctga gctggggtgt tcagtgtttt
     9061 gcacgttatc cggatcacat gaaacagcac gattttttca aaagcgcaat gccggaaggt
     9121 tatgttcaag aacgtaccat cttcttcaaa gatgacggca actataaaac ccgtgccgaa
     9181 gttaaatttg aaggtgatac cctggtgaat cgcattgaac tgaaaggcat cgattttaaa
     9241 gaggatggta atatcctggg ccacaaactg gaatataatg ccattcatgg caacgtgtat
     9301 atcaccgcag ataaacagaa aaacggcatc aaagcaaatt ttggcctgaa ctgcaatatt
     9361 gaagatggtt cagttcagct ggcagatcat tatcagcaga ataccccgat tggtgatggt
     9421 ccggttctgc tgccggataa tcattatctg agcacccaga gcaaactgag caaagatccg
     9481 aatgaaaaac gtgatcacat ggtgctgctg gaatttgtta ccgcagcagg tattaccctg
     9541 ggtatggatg aactgtataa agagctcatg cagtcgatga aagaaacagc ttcgaatatt
     9601 gcagcttctg caaaatctgg catggacaaa accaaagcta ccttggagga aaaggcggag
     9661 aagatgaaga cacgagaccc tgttcagaaa cagatggcta cacaggttaa agaagataag
     9721 atcaatcaag ctgagatgca gaagagagaa acgcgtcagc acaacgcggc catgaaagaa
     9781 gcggctggag ccggaaccgg tttaggtttg gggacggcca ctcactcgac cactggacaa
     9841 gtcggacacg gcactgggac acatcagatg tcggctctgc ctggtcacgg aacgggacaa
     9901 ctgaccgacc gcgttgtgga gggcacggct gtgaccgacc cgattggaag gaacactgga
     9961 actggtcgga caaccgctca taacacacac gttggtggtg gtggtgccac cgggtacgga
    10021 accggcgggg gatatactgg aagatctatg gtgtcaaaag gtgaggaact gtttacaggc
    10081 gtggtgccga tcctggtaga gctggacggg gatgtgaatg gccataaatt cagcgtttca
    10141 ggtgaaggtg agggcgacgc cacgtacgga aaactgacac tgaaattcat ttgcacaaca
    10201 ggtaaactgc ctgtgccttg gcctacactg gtgaccacct ttggttatgg tctgatgtgc
    10261 tttgctcgct atcctgacca catgaaacaa catgatttct ttaaatctgc catgcctgaa
    10321 ggctacgtgc aagagcgcac catttttttc aaagacgatg ggaattacaa aacacgtgcg
    10381 gaggtgaaat tcgagggcga tacactggtt aaccgtatcg agctgaaagg tatcgacttc
    10441 aaagaggacg gaaacattct gggtcataaa ctggaataca actacaacag ccataacgtg
    10501 tacatcatgg ccgacaaaca aaaaaacggg attaaagtga acttcaaaat ccgccacaac
    10561 atcgaagatg gcagcgtgca gctggccgac cactatcaac aaaacacacc gatcggcgac
    10621 ggtcctgtac tgctgcctga caaccactat ctgtcatatc agagcgcact gtcaaaagat
    10681 cctaacgaga aacgcgacca catggttctg ctggaattcg tgacagccgc tggcattaca
    10741 ctgggcatgg acgagctgta caaataaccc gggtaggagc tcatatgaag atgaagatga
    10801 aatatttggt gtgtcaaata aatagcttgt gtgcttaagt ttgtgttttt ttcttggctt
    10861 gttgtgttat gaatttgtgg ctttttctaa tattaaatga atgtaagatc tcattataat
    10921 gaataaacaa atgtttctat aatccattgt gaatgttttg ttggatctct tctgcagcat
    10981 ataactactg tatgtgctat ggtatggact atggaatatg attaaagata aggaattcag
    11041 atcggctgag tggctccttc aacgttgcgg ttctgtcagt tccaaacgta aaacggcttg
    11101 tcccgcgtca tcggcggggg tcataacgtg actcccttaa ttctccgctc atgatcagat
    11161 tgtcgtttcc cgccttcagt ttaaactatc agtgtttgac aggatatatt ggcgggtaaa
    11221 cctaagagaa aagagcgttt attagaataa tcggatattt aaaagggcgt gaaaaggttt
    11281 atccgttcgt ccatttgtat gtgcatgcca accacagggt tccccagatc tggcgccggc
    11341 cagcgagacg agcaagattg gccgccgccc gaaacgatcc gacagcgcgc ccagcacagg
    11401 tgcgcaggca aattgcacca acgcatacag cgccagcaga atgccatagt gggcggtgac
    11461 gtcgttcgag tgaaccagat cgcgcaggag gcccggcagc accggcataa tcaggccgat
    11521 gccgacagcg tcgagcgcga cagtgctcag aattacgatc aggggtatgt tgggtttcac
    11581 gtctggcctc cggaccagcc tccgctggtc cgattgaacg cgcggattct ttatcactga
    11641 taagttggtg gacatattat gtttatcagt gataaagtgt caagcatgac aaagttgcag
    11701 ccgaatacag tgatccgtgc cgccctggac ctgttgaacg aggtcggcgt agacggtctg
    11761 acgacacgca aactggcgga acggttgggg gttcagcagc cggcgcttta ctggcacttc
    11821 aggaacaagc gggcgctgct cgacgcactg gccgaagcca tgctggcgga gaatcatacg
    11881 cattcggtgc cgagagccga cgacgactgg cgctcatttc tgatcgggaa tgcccgcagc
    11941 ttcaggcagg cgctgctcgc ctaccgcgat ggcgcgcgca tccatgccgg cacgcgaccg
    12001 ggcgcaccgc agatggaaac ggccgacgcg cagcttcgct tcctctgcga ggcgggtttt
    12061 tcggccgggg acgccgtcaa tgcgctgatg acaatcagct acttcactgt tggggccgtg
    12121 cttgaggagc aggccggcga cagcgatgcc ggcgagcgcg gcggcaccgt tgaacaggct
    12181 ccgctctcgc cgctgttgcg ggccgcgata gacgccttcg acgaagccgg tccggacgca
    12241 gcgttcgagc agggactcgc ggtgattgtc gatggattgg cgaaaaggag gctcgttgtc
    12301 aggaacgttg aaggaccgag aaagggtgac gattgatcag gaccgctgcc ggagcgcaac
    12361 ccactcacta cagcagagcc atgtagacaa catcccctcc ccctttccac cgcgtcagac
    12421 gcccgtagca gcccgctacg ggctttttca tgccctgccc tagcgtccaa gcctcacggc
    12481 cgcgctcggc ctctctggcg gccttctggc gctcttccgc ttcctcgctc actgactcgc
    12541 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
    12601 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
    12661 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
    12721 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
    12781 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
    12841 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gcttttccgc tgcataaccc
    12901 tgcttcgggg tcattatagc gattttttcg gtatatccat cctttttcgc acgatataca
    12961 ggattttgcc aaagggttcg tgtagacttt ccttggtgta tccaacggcg tcagccgggc
    13021 aggataggtg aagtaggccc acccgcgagc gggtgttcct tcttcactgt cccttattcg
    13081 cacctggcgg tgctcaacgg gaatcctgct ctgcgaggct ggccggctac cgccggcgta
    13141 acagatgagg gcaagcggat ggctgatgaa accaagccaa ccaggaaggg cagcccacct
    13201 atcaaggtgt actgccttcc agacgaacga agagcgattg aggaaaaggc ggcggcggcc
    13261 ggcatgagcc tgtcggccta cctgctggcc gtcggccagg gctacaaaat cacgggcgtc
    13321 gtggactatg agcacgtccg cgagctggcc cgcatcaatg gcgacctggg ccgcctgggc
    13381 ggcctgctga aactctggct caccgacgac ccgcgcacgg cgcggttcgg tgatgccacg
    13441 atcctcgccc tgctggcgaa gatcgaagag aagcaggacg agcttggcaa ggtcatgatg
    13501 ggcgtggtcc gcccgagggc agagccatga cttttttagc cgctaaaacg gccggggggt
    13561 gcgcgtgatt gccaagcacg tccccatgcg ctccatcaag aagagcgact tcgcggagct
    13621 ggtgaagtac atcaccgacg agcaaggcaa gaccgagcgc ctttccgacg ctca
//
