LOCUS       sequence-466037-       15764 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For lentiviral expression of PE7 with blasticidin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15764)
  AUTHORS   Herger M, Kajba CM, Buckley M, Cunha A, Strom M, Findlay GM
  TITLE     High-throughput screening of human genetic variants by pooled prime 
            editing.
  JOURNAL   Cell Genom. 2025 Mar 18:100814. doi: 10.1016/j.xgen.2025.100814.
  PUBMED    40120586
REFERENCE   2  (bases 1 to 15764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Genom.
            2025 Mar 18:100814. doi: 10.1016/j.xgen.2025.100814."
COMMENT     Sequence Label: pLenti_PE7-2A-BSD
FEATURES             Location/Qualifiers
     source          1..15764
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        388..586
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             604..784
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    831..956
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1449..1682
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1867..1911
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2209..2326
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2419..2630
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2686..2706
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2707..6807
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6865..6885
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9664..9684
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9694..9720
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             9730..9786
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             9787..10185
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    10246..10834
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10299..10319)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10717..10728)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10837..10853)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10838..10854)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             11359..11539
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(11565..11582)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11571..11795
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11841..12269
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11928..11947)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12138..12159
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(12278..12298)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      12443..12577
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     12504..12523
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        12639..12686
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             12705..13079
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    13209..13330
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13246..13265)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13300..13319
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(13379..13395)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13379..13395)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13392..13414)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13403..13419
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13427..13433,13434..13451,13452..13457))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13472..13493
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13610..13627)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13781..14369)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13861..13880)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14540..15331,15332..15400))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15163..15182
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15401..15505)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(15580..15599)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
      601 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      661 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      721 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
      781 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
      841 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
      901 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
      961 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1021 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1081 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1141 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1201 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1261 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     1321 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     1381 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     1441 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     1501 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     1561 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     1621 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     1681 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     1741 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     1801 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     1861 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     1921 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     1981 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     2041 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     2101 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
     2161 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
     2221 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2281 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     2341 ggacagcaga gatccagttt ggttaattag ctagctaggt cttgaaagga gtgggaattg
     2401 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
     2461 gaggggtcgg caattgatcc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
     2521 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
     2581 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gaccggttct
     2641 agagccacca tgaaacggac agccgacgga agcgagttcg agtcaccaaa gaagaagcgg
     2701 aaagtcgaca agaagtacag catcggcctg gacatcggca ccaactctgt gggctgggcc
     2761 gtgatcaccg acgagtacaa ggtgcccagc aagaaattca aggtgctggg caacaccgac
     2821 cggcacagca tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc
     2881 gaggccaccc ggctgaagag aaccgccaga agaagataca ccagacggaa gaaccggatc
     2941 tgctatctgc aagagatctt cagcaacgag atggccaagg tggacgacag cttcttccac
     3001 agactggaag agtccttcct ggtggaagag gataagaagc acgagcggca ccccatcttc
     3061 ggcaacatcg tggacgaggt ggcctaccac gagaagtacc ccaccatcta ccacctgaga
     3121 aagaaactgg tggacagcac cgacaaggcc gacctgcggc tgatctatct ggccctggcc
     3181 cacatgatca agttccgggg ccacttcctg atcgagggcg acctgaaccc cgacaacagc
     3241 gacgtggaca agctgttcat ccagctggtg cagacctaca accagctgtt cgaggaaaac
     3301 cccatcaacg ccagcggcgt ggacgccaag gccatcctgt ctgccagact gagcaagagc
     3361 agaaagctgg aaaatctgat cgcccagctg cccggcgaga agaagaatgg cctgttcgga
     3421 aacctgattg ccctgagcct gggcctgacc cccaacttca agagcaactt cgacctggcc
     3481 gaggatgcca aactgcagct gagcaaggac acctacgacg acgacctgga caacctgctg
     3541 gcccagatcg gcgaccagta cgccgacctg tttctggccg ccaagaacct gtccgacgcc
     3601 atcctgctga gcgacatcct gagagtgaac accgagatca ccaaggcccc cctgagcgcc
     3661 tctatgatca agagatacga cgagcaccac caggacctga ccctgctgaa agctctcgtg
     3721 cggcagcagc tgcctgagaa gtacaaagag attttcttcg accagagcaa gaacggctac
     3781 gccggctaca ttgacggcgg agccagccag gaagagttct acaagttcat caagcccatc
     3841 ctggaaaaga tggacggcac cgaggaactg ctcgtgaagc tgaagagaga ggacctgctg
     3901 cggaagcagc ggaccttcga caacggcagc atcccccacc agatccacct gggagagctg
     3961 cacgccattc tgcggcggca ggaagatttt tacccattcc tgaaggacaa ccgggaaaag
     4021 atcgagaaga tcctgacctt ccgcatcccc tactacgtgg gccctctggc caggggaaac
     4081 agcagattcg cctggatgac cagaaagagc gaggaaacca tcaccccctg gaacttcgag
     4141 gaagtggtgg acaagggcgc ttccgcccag agcttcatcg agcggatgac caacttcgat
     4201 aagaacctgc ccaacgagaa ggtgctgccc aagcacagcc tgctgtacga gtacttcacc
     4261 gtgtataacg agctgaccaa agtgaaatac gtgaccgagg gaatgagaaa gcccgccttc
     4321 ctgagcggcg agcagaaaaa ggccatcgtg gacctgctgt tcaagaccaa ccggaaagtg
     4381 accgtgaagc agctgaaaga ggactacttc aagaaaatcg agtgcttcga ctccgtggaa
     4441 atctccggcg tggaagatcg gttcaacgcc tccctgggca cataccacga tctgctgaaa
     4501 attatcaagg acaaggactt cctggacaat gaggaaaacg aggacattct ggaagatatc
     4561 gtgctgaccc tgacactgtt tgaggacaga gagatgatcg aggaacggct gaaaacctat
     4621 gcccacctgt tcgacgacaa agtgatgaag cagctgaagc ggcggagata caccggctgg
     4681 ggcaggctga gccggaagct gatcaacggc atccgggaca agcagtccgg caagacaatc
     4741 ctggatttcc tgaagtccga cggcttcgcc aacagaaact tcatgcagct gatccacgac
     4801 gacagcctga cctttaaaga ggacatccag aaagcccagg tgtccggcca gggcgatagc
     4861 ctgcacgagc acattgccaa tctggccggc agccccgcca ttaagaaggg catcctgcag
     4921 acagtgaagg tggtggacga gctcgtgaaa gtgatgggcc ggcacaagcc cgagaacatc
     4981 gtgatcgaaa tggccagaga gaaccagacc acccagaagg gacagaagaa cagccgcgag
     5041 agaatgaagc ggatcgaaga gggcatcaaa gagctgggca gccagatcct gaaagaacac
     5101 cccgtggaaa acacccagct gcagaacgag aagctgtacc tgtactacct gcagaatggg
     5161 cgggatatgt acgtggacca ggaactggac atcaaccggc tgtccgacta cgatgtggac
     5221 gctatcgtgc ctcagagctt tctgaaggac gactccatcg acaacaaggt gctgaccaga
     5281 agcgacaaga accggggcaa gagcgacaac gtgccctccg aagaggtcgt gaagaagatg
     5341 aagaactact ggcggcagct gctgaacgcc aagctgatta cccagagaaa gttcgacaat
     5401 ctgaccaagg ccgagagagg cggcctgagc gaactggata aggccggctt catcaagaga
     5461 cagctggtgg aaacccggca gatcacaaag cacgtggcac agatcctgga ctcccggatg
     5521 aacactaagt acgacgagaa tgacaagctg atccgggaag tgaaagtgat caccctgaag
     5581 tccaagctgg tgtccgattt ccggaaggat ttccagtttt acaaagtgcg cgagatcaac
     5641 aactaccacc acgcccacga cgcctacctg aacgccgtcg tgggaaccgc cctgatcaaa
     5701 aagtacccta agctggaaag cgagttcgtg tacggcgact acaaggtgta cgacgtgcgg
     5761 aagatgatcg ccaagagcga gcaggaaatc ggcaaggcta ccgccaagta cttcttctac
     5821 agcaacatca tgaacttttt caagaccgag attaccctgg ccaacggcga gatccggaag
     5881 cggcctctga tcgagacaaa cggcgaaacc ggggagatcg tgtgggataa gggccgggat
     5941 tttgccaccg tgcggaaagt gctgagcatg ccccaagtga atatcgtgaa aaagaccgag
     6001 gtgcagacag gcggcttcag caaagagtct atcctgccca agaggaacag cgataagctg
     6061 atcgccagaa agaaggactg ggaccctaag aagtacggcg gcttcgacag ccccaccgtg
     6121 gcctattctg tgctggtggt ggccaaagtg gaaaagggca agtccaagaa actgaagagt
     6181 gtgaaagagc tgctggggat caccatcatg gaaagaagca gcttcgagaa gaatcccatc
     6241 gactttctgg aagccaaggg ctacaaagaa gtgaaaaagg acctgatcat caagctgcct
     6301 aagtactccc tgttcgagct ggaaaacggc cggaagagaa tgctggcctc tgccggcgaa
     6361 ctgcagaagg gaaacgaact ggccctgccc tccaaatatg tgaacttcct gtacctggcc
     6421 agccactatg agaagctgaa gggctccccc gaggataatg agcagaaaca gctgtttgtg
     6481 gaacagcaca agcactacct ggacgagatc atcgagcaga tcagcgagtt ctccaagaga
     6541 gtgatcctgg ccgacgctaa tctggacaaa gtgctgtccg cctacaacaa gcaccgggat
     6601 aagcccatca gagagcaggc cgagaatatc atccacctgt ttaccctgac caatctggga
     6661 gcccctgccg ccttcaagta ctttgacacc accatcgacc ggaagaggta caccagcacc
     6721 aaagaggtgc tggacgccac cctgatccac cagagcatca ccggcctgta cgagacacgg
     6781 atcgacctgt ctcagctggg aggtgactcc ggcggaagct ctggtggcag caagcggacc
     6841 gccgacggct ctgaattcga gagccctaag aagaaaagaa aggtgagcgg aggctctagc
     6901 ggcggaagca ccctgaacat tgaagacgag tatagactgc atgaaacaag caaggaaccc
     6961 gatgtgtccc tgggctccac ctggctgtcc gactttcccc aggcctgggc cgagacagga
     7021 ggaatgggcc tggccgtgcg gcaggcaccc ctgatcatcc ctctgaaggc cacctctaca
     7081 cccgtgagca tcaagcagta ccctatgtct caggaggcca gactgggcat caagcctcac
     7141 atccagaggc tgctggacca gggcatcctg gtgccatgcc agagcccctg gaacacacca
     7201 ctgctgcccg tgaagaagcc aggcaccaat gactatagac ccgtgcagga tctgagagag
     7261 gtgaacaaga gggtggagga tatccacccc accgtgccca acccttacaa tctgctgtcc
     7321 ggcctgcccc cttctcacca gtggtataca gtgctggacc tgaaggatgc cttcttttgt
     7381 ctgagactgc accctaccag ccagccactg ttcgcctttg agtggaggga ccctgagatg
     7441 ggcatctctg gccagctgac ctggacacgc ctgcctcagg gcttcaagaa tagcccaaca
     7501 ctgtttaacg aggccctgca ccgcgacctg gcagatttcc ggatccagca cccagatctg
     7561 atcctgctgc agtacgtgga cgatctgctg ctggccgcca ccagcgagct ggattgccag
     7621 cagggaacac gcgccctgct gcagaccctg ggaaacctgg gatatagggc atccgccaag
     7681 aaggcccaga tctgtcagaa gcaggtgaag tacctgggct atctgctgaa ggagggccag
     7741 agatggctga cagaggccag gaaggagaca gtgatgggcc agccaacacc caagacccca
     7801 agacagctga gggagttcct gggcaaagca ggattttgca ggctgttcat cccaggattc
     7861 gcagagatgg cagcacctct gtacccactg accaagccgg gcaccctgtt taattggggc
     7921 cctgaccagc agaaggccta tcaggagatc aagcaggccc tgctgacagc accagccctg
     7981 ggcctgccag acctgaccaa gcctttcgag ctgtttgtgg atgagaagca gggctacgcc
     8041 aagggcgtgc tgacccagaa gctgggacca tggagacggc ccgtggccta tctgtccaag
     8101 aagctggacc cagtggcagc aggatggcca ccatgcctga ggatggtggc agcaatcgcc
     8161 gtgctgacaa aggatgccgg caagctgacc atgggacagc cactggtcat cctggcacca
     8221 cacgcagtgg aggccctggt gaagcagcct ccagatcgct ggctgtctaa cgcccggatg
     8281 acacactacc aggccctgct gctggacacc gatcgcgtgc agtttggccc tgtggtggcc
     8341 ctgaatccag ccaccctgct gcctctgcca gaggagggcc tgcagcacaa ctgtctggac
     8401 atcctggcag aggcacacgg aacaaggcca gacctgaccg atcagcccct gcctgacgcc
     8461 gatcacacat ggtataccga tggaagctcc ctgctgcagg agggccagag gaaggcagga
     8521 gcagcagtga ccacagagac agaagtgatc tgggccaagg ccctgccagc aggcacatcc
     8581 gcccagcggg ccgagctgat cgccctgacc caggccctga agatggccga gggcaagaag
     8641 ctgaacgtgt acacagactc cagatatgcc ttcgccaccg cacacatcca cggagagatc
     8701 tacaggcgcc ggggctggct gacctctgag ggcaaggaga tcaagaacaa ggatgagatc
     8761 ctggccctgc tgaaggccct gtttctgccc aagcggctga gcatcatcca ctgtcctgga
     8821 caccagaagg gacactccgc cgaggcaagg ggcaatcgga tggccgacca ggccgccaga
     8881 aaggctgcta ttactgaaac tcccgacact tccactctgc tgattgaaaa ctcctcccct
     8941 tccggaggat ctagcggagg ctcctctggc tctgagacac ctggcacaag cgagagcgca
     9001 acacctgaaa gcagcggggg cagcagcggg gggtcaatgg ctgaaaatgg tgataatgaa
     9061 aagatggctg ccctggaggc caaaatctgt catcaaattg agtattattt tggcgacttc
     9121 aatttgccac gggacaagtt tctaaaggaa cagataaaac tggatgaagg ctgggtacct
     9181 ttggagataa tgataaaatt caacaggttg aaccgtctaa caacagactt taatgtaatt
     9241 gtggaagcat tgagcaaatc caaggcagaa ctcatggaaa tcagtgaaga taaaactaaa
     9301 atcagaaggt ctccaagcaa acccctacct gaagtgactg atgagtataa aaatgatgta
     9361 aaaaacagat ctgtttatat taaaggcttc ccaactgatg caactcttga tgacataaaa
     9421 gaatggttag aagataaagg tcaagtacta aatattcaga tgagaagaac attgcataaa
     9481 gcatttaagg gatcaatttt tgttgtgttt gatagcattg aatctgctaa gaaatttgta
     9541 gagacccctg gccagaagta caaagaaaca gacctgctaa tacttttcaa ggacgattac
     9601 tttgccaaaa aaaatgaatc tggcggctca aaaagaaccg ccgacggcag cgaattcgag
     9661 tctcccaaga agaagaggaa agtcggctct ggccctgccg ctaagagagt gaagctggac
     9721 ggatccggcg caacaaactt ctctctgctg aaacaagccg gagatgtcga agagaatcct
     9781 ggaccgatgg ccaagccttt gtctcaagaa gaatccaccc tcattgaaag agcaacggct
     9841 acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc tctctctagc
     9901 gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa
     9961 ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg tatcgtcgcg
    10021 atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca ggtgcttctc
    10081 gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc gacggcagtt
    10141 gggattcgtg aattgctgcc ctctggttat gtgtgggagg gctaagccgt gacctacatg
    10201 ctcgaagggc gtatgcgcca cgaattcgat atcaagctta tcggtaatca acctctggat
    10261 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
    10321 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
    10381 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
    10441 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
    10501 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
    10561 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
    10621 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
    10681 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
    10741 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
    10801 acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta
    10861 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg
    10921 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga
    10981 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
    11041 gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca
    11101 caaggctact tccctgattg gcagaactac acaccagggc cagggatcag atatccactg
    11161 acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga agaagccaat
    11221 gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag
    11281 agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag
    11341 ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
    11401 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
    11461 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
    11521 gtgtggaaaa tctctagcag ggcccgttta aacccgctga tcagcctcga ctgtgccttc
    11581 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
    11641 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
    11701 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
    11761 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
    11821 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
    11881 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
    11941 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
    12001 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
    12061 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
    12121 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
    12181 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
    12241 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
    12301 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
    12361 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
    12421 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccagttccgc ccattctccg
    12481 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
    12541 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
    12601 ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat
    12661 agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca
    12721 gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc
    12781 ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg
    12841 tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg
    12901 tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact
    12961 tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt
    13021 tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac
    13081 acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
    13141 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
    13201 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
    13261 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
    13321 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
    13381 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
    13441 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
    13501 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
    13561 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
    13621 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
    13681 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
    13741 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
    13801 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
    13861 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
    13921 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
    13981 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
    14041 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    14101 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
    14161 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
    14221 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
    14281 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
    14341 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
    14401 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
    14461 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
    14521 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
    14581 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
    14641 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
    14701 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
    14761 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    14821 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
    14881 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
    14941 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
    15001 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    15061 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    15121 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    15181 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    15241 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    15301 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    15361 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    15421 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    15481 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
    15541 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
    15601 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
    15661 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
    15721 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tata
//
