LOCUS       sequence-466038-       17408 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For lentiviral expression of PEmax with MLH1dn and blasticidin 
            resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17408)
  AUTHORS   Herger M, Kajba CM, Buckley M, Cunha A, Strom M, Findlay GM
  TITLE     High-throughput screening of human genetic variants by pooled prime 
            editing.
  JOURNAL   Cell Genom. 2025 Mar 18:100814. doi: 10.1016/j.xgen.2025.100814.
  PUBMED    40120586
REFERENCE   2  (bases 1 to 17408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Genom.
            2025 Mar 18:100814. doi: 10.1016/j.xgen.2025.100814."
COMMENT     Sequence Label: pLenti_PEmax-2A-MLH1dn-2A-BSD
FEATURES             Location/Qualifiers
     source          1..17408
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(46..65)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        237..616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..816
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     771..791
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             834..1014
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1061..1186
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1679..1912
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2097..2141
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2439..2556
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2649..2860
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2916..2936
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2937..7037
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7095..7115
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9216..9236
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9246..9272
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             9282..9338
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             11604..11660
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             11661..12059
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    12120..12708
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(12173..12193)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(12591..12602)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(12711..12727)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12712..12728)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             13233..13413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(13439..13456)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    13445..13669
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      13715..14143
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13802..13821)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14012..14033
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(14152..14172)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      14317..14451
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     14378..14397
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        14513..14560
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             14579..14953
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    15083..15204
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(15120..15139)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     15174..15193
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(15253..15269)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15253..15269)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(15266..15288)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    15277..15293
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(15301..15307,15308..15325,15326..15331))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15346..15367
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(15484..15501)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15655..16243)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15735..15754)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(16414..17205,17206..17274))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     17037..17056
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(17275..17379)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
      841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     1021 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     1081 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     1141 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     1201 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1261 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1321 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1381 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1441 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1501 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     1561 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     1621 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     1681 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     1741 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     1801 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     1861 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     1921 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     1981 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     2041 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     2101 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     2161 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     2221 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     2281 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     2341 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg
     2401 cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     2461 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2521 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     2581 gatccagttt ggttaattag ctagctaggt cttgaaagga gtgggaattg gctccggtgc
     2641 ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg
     2701 caattgatcc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta
     2761 ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt
     2821 gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gaccggttct agagccacca
     2881 tgaaacggac agccgacgga agcgagttcg agtcaccaaa gaagaagcgg aaagtcgaca
     2941 agaagtacag catcggcctg gacatcggca ccaactctgt gggctgggcc gtgatcaccg
     3001 acgagtacaa ggtgcccagc aagaaattca aggtgctggg caacaccgac cggcacagca
     3061 tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc
     3121 ggctgaagag aaccgccaga agaagataca ccagacggaa gaaccggatc tgctatctgc
     3181 aagagatctt cagcaacgag atggccaagg tggacgacag cttcttccac agactggaag
     3241 agtccttcct ggtggaagag gataagaagc acgagcggca ccccatcttc ggcaacatcg
     3301 tggacgaggt ggcctaccac gagaagtacc ccaccatcta ccacctgaga aagaaactgg
     3361 tggacagcac cgacaaggcc gacctgcggc tgatctatct ggccctggcc cacatgatca
     3421 agttccgggg ccacttcctg atcgagggcg acctgaaccc cgacaacagc gacgtggaca
     3481 agctgttcat ccagctggtg cagacctaca accagctgtt cgaggaaaac cccatcaacg
     3541 ccagcggcgt ggacgccaag gccatcctgt ctgccagact gagcaagagc agaaagctgg
     3601 aaaatctgat cgcccagctg cccggcgaga agaagaatgg cctgttcgga aacctgattg
     3661 ccctgagcct gggcctgacc cccaacttca agagcaactt cgacctggcc gaggatgcca
     3721 aactgcagct gagcaaggac acctacgacg acgacctgga caacctgctg gcccagatcg
     3781 gcgaccagta cgccgacctg tttctggccg ccaagaacct gtccgacgcc atcctgctga
     3841 gcgacatcct gagagtgaac accgagatca ccaaggcccc cctgagcgcc tctatgatca
     3901 agagatacga cgagcaccac caggacctga ccctgctgaa agctctcgtg cggcagcagc
     3961 tgcctgagaa gtacaaagag attttcttcg accagagcaa gaacggctac gccggctaca
     4021 ttgacggcgg agccagccag gaagagttct acaagttcat caagcccatc ctggaaaaga
     4081 tggacggcac cgaggaactg ctcgtgaagc tgaagagaga ggacctgctg cggaagcagc
     4141 ggaccttcga caacggcagc atcccccacc agatccacct gggagagctg cacgccattc
     4201 tgcggcggca ggaagatttt tacccattcc tgaaggacaa ccgggaaaag atcgagaaga
     4261 tcctgacctt ccgcatcccc tactacgtgg gccctctggc caggggaaac agcagattcg
     4321 cctggatgac cagaaagagc gaggaaacca tcaccccctg gaacttcgag gaagtggtgg
     4381 acaagggcgc ttccgcccag agcttcatcg agcggatgac caacttcgat aagaacctgc
     4441 ccaacgagaa ggtgctgccc aagcacagcc tgctgtacga gtacttcacc gtgtataacg
     4501 agctgaccaa agtgaaatac gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg
     4561 agcagaaaaa ggccatcgtg gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc
     4621 agctgaaaga ggactacttc aagaaaatcg agtgcttcga ctccgtggaa atctccggcg
     4681 tggaagatcg gttcaacgcc tccctgggca cataccacga tctgctgaaa attatcaagg
     4741 acaaggactt cctggacaat gaggaaaacg aggacattct ggaagatatc gtgctgaccc
     4801 tgacactgtt tgaggacaga gagatgatcg aggaacggct gaaaacctat gcccacctgt
     4861 tcgacgacaa agtgatgaag cagctgaagc ggcggagata caccggctgg ggcaggctga
     4921 gccggaagct gatcaacggc atccgggaca agcagtccgg caagacaatc ctggatttcc
     4981 tgaagtccga cggcttcgcc aacagaaact tcatgcagct gatccacgac gacagcctga
     5041 cctttaaaga ggacatccag aaagcccagg tgtccggcca gggcgatagc ctgcacgagc
     5101 acattgccaa tctggccggc agccccgcca ttaagaaggg catcctgcag acagtgaagg
     5161 tggtggacga gctcgtgaaa gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa
     5221 tggccagaga gaaccagacc acccagaagg gacagaagaa cagccgcgag agaatgaagc
     5281 ggatcgaaga gggcatcaaa gagctgggca gccagatcct gaaagaacac cccgtggaaa
     5341 acacccagct gcagaacgag aagctgtacc tgtactacct gcagaatggg cgggatatgt
     5401 acgtggacca ggaactggac atcaaccggc tgtccgacta cgatgtggac gctatcgtgc
     5461 ctcagagctt tctgaaggac gactccatcg acaacaaggt gctgaccaga agcgacaaga
     5521 accggggcaa gagcgacaac gtgccctccg aagaggtcgt gaagaagatg aagaactact
     5581 ggcggcagct gctgaacgcc aagctgatta cccagagaaa gttcgacaat ctgaccaagg
     5641 ccgagagagg cggcctgagc gaactggata aggccggctt catcaagaga cagctggtgg
     5701 aaacccggca gatcacaaag cacgtggcac agatcctgga ctcccggatg aacactaagt
     5761 acgacgagaa tgacaagctg atccgggaag tgaaagtgat caccctgaag tccaagctgg
     5821 tgtccgattt ccggaaggat ttccagtttt acaaagtgcg cgagatcaac aactaccacc
     5881 acgcccacga cgcctacctg aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta
     5941 agctggaaag cgagttcgtg tacggcgact acaaggtgta cgacgtgcgg aagatgatcg
     6001 ccaagagcga gcaggaaatc ggcaaggcta ccgccaagta cttcttctac agcaacatca
     6061 tgaacttttt caagaccgag attaccctgg ccaacggcga gatccggaag cggcctctga
     6121 tcgagacaaa cggcgaaacc ggggagatcg tgtgggataa gggccgggat tttgccaccg
     6181 tgcggaaagt gctgagcatg ccccaagtga atatcgtgaa aaagaccgag gtgcagacag
     6241 gcggcttcag caaagagtct atcctgccca agaggaacag cgataagctg atcgccagaa
     6301 agaaggactg ggaccctaag aagtacggcg gcttcgacag ccccaccgtg gcctattctg
     6361 tgctggtggt ggccaaagtg gaaaagggca agtccaagaa actgaagagt gtgaaagagc
     6421 tgctggggat caccatcatg gaaagaagca gcttcgagaa gaatcccatc gactttctgg
     6481 aagccaaggg ctacaaagaa gtgaaaaagg acctgatcat caagctgcct aagtactccc
     6541 tgttcgagct ggaaaacggc cggaagagaa tgctggcctc tgccggcgaa ctgcagaagg
     6601 gaaacgaact ggccctgccc tccaaatatg tgaacttcct gtacctggcc agccactatg
     6661 agaagctgaa gggctccccc gaggataatg agcagaaaca gctgtttgtg gaacagcaca
     6721 agcactacct ggacgagatc atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg
     6781 ccgacgctaa tctggacaaa gtgctgtccg cctacaacaa gcaccgggat aagcccatca
     6841 gagagcaggc cgagaatatc atccacctgt ttaccctgac caatctggga gcccctgccg
     6901 ccttcaagta ctttgacacc accatcgacc ggaagaggta caccagcacc aaagaggtgc
     6961 tggacgccac cctgatccac cagagcatca ccggcctgta cgagacacgg atcgacctgt
     7021 ctcagctggg aggtgactcc ggcggaagct ctggtggcag caagcggacc gccgacggct
     7081 ctgaattcga gagccctaag aagaaaagaa aggtgagcgg aggctctagc ggcggaagca
     7141 ccctgaacat tgaagacgag tatagactgc atgaaacaag caaggaaccc gacgtgtccc
     7201 tgggctccac ctggctgtcc gactttcccc aggcctgggc cgagacagga ggaatgggcc
     7261 tggccgtgcg gcaggcaccc ctgatcatcc ctctgaaggc cacctctaca cccgtgagca
     7321 tcaagcagta ccctatgtct caggaggcca gactgggcat caagcctcac atccagaggc
     7381 tgctggacca gggcatcctg gtgccatgcc agagcccctg gaacacacca ctgctgcccg
     7441 tgaagaagcc aggcaccaat gactatagac ccgtgcagga tctgagagag gtgaacaaga
     7501 gggtggagga tatccacccc accgtgccca acccttacaa tctgctgtcc ggcctgcccc
     7561 cttctcacca gtggtataca gtgctggacc tgaaggatgc cttcttttgt ctgagactgc
     7621 accctaccag ccagccactg ttcgcctttg agtggaggga ccctgagatg ggcatctctg
     7681 gccagctgac ctggacacgc ctgcctcagg gcttcaagaa tagcccaaca ctgtttaacg
     7741 aggccctgca ccgcgacctg gcagatttcc ggatccagca cccagatctg atcctgctgc
     7801 agtacgtgga cgatctgctg ctggccgcca ccagcgagct ggattgccag cagggaacac
     7861 gcgccctgct gcagaccctg ggaaacctgg gatatagggc atccgccaag aaggcccaga
     7921 tctgtcagaa gcaggtgaag tacctgggct atctgctgaa ggagggccag agatggctga
     7981 cagaggccag gaaggagaca gtgatgggcc agccaacacc caagacccca agacagctga
     8041 gggagttcct gggcaaagca ggattttgca ggctgttcat cccaggattc gcagagatgg
     8101 cagcacctct gtacccactg accaagccgg gcaccctgtt taattggggc cctgaccagc
     8161 agaaggccta tcaggagatc aagcaggccc tgctgacagc accagccctg ggcctgccag
     8221 acctgaccaa gcctttcgag ctgtttgtgg atgagaagca gggctacgcc aagggcgtgc
     8281 tgacccagaa gctgggacca tggagacggc ccgtggccta tctgtccaag aagctggacc
     8341 cagtggcagc aggatggcca ccatgcctga ggatggtggc agcaatcgcc gtgctgacaa
     8401 aggatgccgg caagctgacc atgggacagc cactggtcat cctggcacca cacgcagtgg
     8461 aggccctggt gaagcagcct ccagatcgct ggctgtctaa cgcccggatg acacactacc
     8521 aggccctgct gctggacacc gatcgcgtgc agtttggccc tgtggtggcc ctgaatccag
     8581 ccaccctgct gcctctgcca gaggagggcc tgcagcacaa ctgtctggac atcctggcag
     8641 aggcacacgg aacaaggcca gacctgaccg atcagcccct gcctgacgcc gatcacacat
     8701 ggtataccga tggaagctcc ctgctgcagg agggccagag gaaggcagga gcagcagtga
     8761 ccacagagac agaagtgatc tgggccaagg ccctgccagc aggcacatcc gcccagcggg
     8821 ccgagctgat cgccctgacc caggccctga agatggccga gggcaagaag ctgaacgtgt
     8881 acacagactc cagatatgcc ttcgccaccg cacacatcca cggagagatc tacaggcgcc
     8941 ggggctggct gacctctgag ggcaaggaga tcaagaacaa ggatgagatc ctggccctgc
     9001 tgaaggccct gtttctgccc aagcggctga gcatcatcca ctgtcctgga caccagaagg
     9061 gacactccgc cgaggcaagg ggcaatcgga tggccgacca ggccgccaga aaggctgcta
     9121 ttactgaaac tcccgacact tccactctgc tgattgaaaa ctcctcccct tctggcggct
     9181 caaaaagaac cgccgacggc agcgaattcg agtctcccaa gaagaagagg aaagtcggct
     9241 ctggccctgc cgctaagaga gtgaagctgg acggaagcgg agctactaac ttcagcctgc
     9301 tgaagcaggc tggagacgtg gaggagaacc ctggacctag cttcgttgct ggagtcatcc
     9361 ggagactgga cgagacagtg gtgaacagaa ttgccgccgg cgaggtgatc cagagacctg
     9421 ccaatgcaat aaaggagatg atcgagaact gtctggacgc caagtccaca agcattcagg
     9481 tgatcgtgaa ggagggcgga ctgaagctga tccagatcca agacaacggc acaggcatca
     9541 gaaaggaaga tctggacatc gtgtgtgaac ggttcaccac atctaagctg cagtcttttg
     9601 aggatctggc ctctatcagt acctacggct tcagaggcga ggccctggcc agcatcagcc
     9661 acgtggccca tgtgaccatc accaccaaaa ccgccgacgg caaatgcgct tatcgcgcta
     9721 gctacagcga cggcaagctg aaagccccgc caaagccttg cgccggcaac cagggtacac
     9781 agataacagt ggaggatctg ttctacaaca tcgccacccg gagaaaggcc ctgaaaaatc
     9841 ccagcgagga gtacggcaag atcctggaag tcgtcggcag atactccgtg cacaacgccg
     9901 gaatcagctt tagcgtaaag aagcagggag aaaccgtggc cgatgtgcgc accctgccaa
     9961 atgccagcac cgtggataac atcagaagca ttttcggaaa tgccgtgtcc agagaactga
    10021 tcgagatcgg ctgcgaagat aagaccctgg cttttaagat gaacggctac atcagcaacg
    10081 ccaattactc tgtgaagaag tgcatctttc ttctgttcat caaccacaga ctggtggaaa
    10141 gcaccagcct gcggaaagcc atcgagacag tgtacgccgc ctacctgcct aagaacaccc
    10201 accccttcct gtacctgagc ctcgagatca gccctcagaa cgtggacgtc aatgtgcatc
    10261 ctacaaagca cgaggtgcac ttcctgcacg aggaaagcat cctggaaaga gtgcagcagc
    10321 acattgagag caagctgctg ggctctaaca gcagcagaat gtacttcaca cagaccctgc
    10381 tgcctggcct ggccggcccc tcaggcgaaa tggttaagtc cacaacctct ctgacctcat
    10441 ccagcaccag cggttcttcc gataaggtgt acgcccacca gatggtgcgg accgactctc
    10501 gggagcagaa gctggacgcc tttctgcaac ctctgagcaa acctctgagc tctcagcctc
    10561 aggccatcgt gaccgaggac aagacagata tctcctccgg ccgtgccaga cagcaggacg
    10621 aagaaatgct cgagctgcca gctcctgccg aggtggccgc caagaaccag agcctggagg
    10681 gagataccac aaagggcacc agcgaaatga gcgagaagcg gggccctacc tccagcaacc
    10741 ccagaaaacg gcaccgggag gacagcgacg tggaaatggt ggaggacgac agcagaaagg
    10801 aaatgacagc cgcttgtacc cctagaagaa gaatcatcaa cctgacctcc gtgctgagcc
    10861 tgcaggagga gatcaacgag cagggccacg aggtgctgag agagatgctg cacaatcaca
    10921 gcttcgtggg ctgcgtgaac cctcaatggg ccctggctca gcatcaaaca aagctgtacc
    10981 tgctgaacac caccaagctg agcgaagagc tgttctacca gatcctcatc tacgacttcg
    11041 ccaacttcgg cgtgctacgc ctgagcgagc ccgcccctct gtttgacctg gccatgctgg
    11101 ctctggatag cccagaaagc ggctggacag aagaggacgg acctaaagag gggctggctg
    11161 aatacatcgt ggagttcctg aagaaaaagg ccgagatgct ggccgactac ttttctctgg
    11221 aaatcgacga ggaaggcaac ctgatcggcc tgcctctgct gatcgataac tacgtgcctc
    11281 ccctggaagg cctgcccatc ttcatcctga gactggctac agaggtgaac tgggacgagg
    11341 aaaaggaatg cttcgagtct ctgagcaagg agtgcgccat gttctatagc atcagaaaac
    11401 agtacatctc tgaagagagc actctgtctg gccagcagag tgaagtgccc ggaagcatcc
    11461 ccaacagctg gaagtggacc gtggaacaca tcgtgtacaa ggccctgcgg agccacattc
    11521 tccctcctaa gcacttcacc gaggacggca acatcctgca gctggccaac ctgcccgacc
    11581 tttataaggt tttcggatcc ggcgcaacaa acttctctct gctgaaacaa gccggagatg
    11641 tcgaagagaa tcctggaccg atggccaagc ctttgtctca agaagaatcc accctcattg
    11701 aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg
    11761 cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg
    11821 gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga
    11881 cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc
    11941 gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac
    12001 agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag
    12061 ccgtgaccta catgctcgaa gggcgtatgc gccacgaatt cgatatcaag cttatcggta
    12121 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
    12181 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
    12241 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
    12301 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
    12361 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
    12421 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
    12481 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
    12541 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
    12601 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
    12661 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc
    12721 gacctcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg
    12781 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc
    12841 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
    12901 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt
    12961 ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca gggccaggga
    13021 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag caagagaagg
    13081 tagaagaagc caatgaagga gagaacaccc gcttgttaca ccctgtgagc ctgcatggga
    13141 tggatgaccc ggagagagaa gtattagagt ggaggtttga cagccgccta gcatttcatc
    13201 acatggcccg agagctgcat ccggactgta ctgggtctct ctggttagac cagatctgag
    13261 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
    13321 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
    13381 gaccctttta gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg ctgatcagcc
    13441 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
    13501 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
    13561 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
    13621 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
    13681 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
    13741 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
    13801 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
    13861 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
    13921 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
    13981 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
    14041 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
    14101 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
    14161 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
    14221 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
    14281 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccagtt
    14341 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
    14401 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
    14461 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa
    14521 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat
    14581 ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga
    14641 gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt
    14701 ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa
    14761 caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt
    14821 cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc
    14881 gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga
    14941 ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt
    15001 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
    15061 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
    15121 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    15181 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
    15241 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
    15301 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
    15361 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
    15421 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
    15481 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
    15541 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
    15601 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
    15661 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
    15721 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    15781 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    15841 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    15901 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    15961 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    16021 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    16081 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    16141 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    16201 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    16261 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
    16321 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
    16381 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
    16441 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    16501 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    16561 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    16621 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    16681 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    16741 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    16801 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    16861 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    16921 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    16981 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    17041 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    17101 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    17161 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    17221 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    17281 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    17341 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    17401 acctgacg
//
