LOCUS       sequence-466047-        8973 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of human GDF11 with 
            C-terminal V5 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8973)
  AUTHORS   Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA
  TITLE     Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor 
            Sequestration in Triple-Negative Breast Cancer.
  JOURNAL   Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 
            10.1016/j.devcel.2017.10.027.
  PUBMED    29161592
REFERENCE   2  (bases 1 to 8973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.10"; journalName: "Dev Cell"; date: 
            "2017-11-20- 20"; volume: "43"; issue: "4"; pages: "418-435.e13"
COMMENT     Sequence Label: pLX304 GDF11-V5 blast
FEATURES             Location/Qualifiers
     source          1..8973
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    531..555
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1807..1831)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1833..1874
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    1916..2504
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1969..1989)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2387..2398)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2576..2809
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2887..3008
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2924..2943)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2978..2997
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3048..3183
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3203..3222)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3204..3222)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3232..3249)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3232..3248)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3241..3263)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3390..3845
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3477..3496)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3687..3708
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3871..3975
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3976..4044,4045..4836)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4194..4213)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5007..5595
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5496..5515
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5749..5766
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5883..5904
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5919..5924,5925..5942,5943..5949)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5957..5973
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5962..5984
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5981..5997
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5981..5997
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6016..6036
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6018..6036
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6064..6290
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6291..6471
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6518..6643
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7136..7369
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7554..7598
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7773..8273
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8192..8210
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8285..8680
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8740..8857
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ctggctaact gtcgggatca acaagtttgt
      541 acaaaaaagc agaagggccg tcaaggccca ccatggtgct cgcggccccg ctgctgctgg
      601 gcttcctgct cctcgccctg gagctgcggc cccgggggga ggcggccgag ggccccgcgg
      661 cggcggcggc ggcggcggcg gcggcggcag cggcgggggt cgggggggag cgctccagcc
      721 ggccagcccc gtccgtggcg cccgagccgg acggctgccc cgtgtgcgtt tggcggcagc
      781 acagccgcga gctgcgccta gagagcatca agtcgcagat cttgagcaaa ctgcggctca
      841 aggaggcgcc caacatcagc cgcgaggtgg tgaagcagct gctgcccaag gcgccgccgc
      901 tgcagcagat cctggaccta cacgacttcc agggcgacgc gctgcagccc gaggacttcc
      961 tggaggagga cgagtaccac gccaccaccg agaccgtcat tagcatggcc caggagacgg
     1021 acccagcagt acagacagat ggcagccctc tctgctgcca ttttcacttc agccccaagg
     1081 tgatgttcac aaaggtactg aaggcccagc tgtgggtgta cctacggcct gtaccccgcc
     1141 cagccacagt ctacctgcag atcttgcgac taaaacccct aactggggaa gggaccgcag
     1201 ggggaggggg cggaggccgg cgtcacatcc gtatccgctc actgaagatt gagctgcact
     1261 cacgctcagg ccattggcag agcatcgact tcaagcaagt gctacacagc tggttccgcc
     1321 agccacagag caactggggc atcgagatca acgcctttga tcccagtggc acagacctgg
     1381 ctgtcacctc cctggggccg ggagccgagg ggctgcatcc attcatggag cttcgagtcc
     1441 tagagaacac aaaacgttcc cggcggaacc tgggtctgga ctgcgacgag cactcaagcg
     1501 agtcccgctg ctgccgatat cccctcacag tggactttga ggctttcggc tgggactgga
     1561 tcatcgcacc taagcgctac aaggccaact actgctccgg ccagtgcgag tacatgttca
     1621 tgcaaaaata tccgcatacc catttggtgc agcaggccaa tccaagaggc tctgctgggc
     1681 cctgttgtac ccccaccaag atgtccccaa tcaacatgct ctacttcaat gacaagcagc
     1741 agattatcta cggcaagatc cctggcatgg tggtggatcg ctgtggctgc tcttcaggcc
     1801 tcatgggccc agctttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg
     1861 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca
     1921 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     1981 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     2041 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     2101 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     2161 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     2221 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     2281 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     2341 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     2401 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     2461 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca
     2521 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     2581 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     2641 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     2701 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     2761 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt
     2821 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     2881 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     2941 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     3001 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     3061 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     3121 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     3181 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     3241 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     3301 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     3361 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     3421 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     3481 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     3541 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     3601 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     3661 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     3721 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     3781 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     3841 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     3901 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     3961 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     4021 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     4081 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     4141 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     4201 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     4261 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     4321 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     4381 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     4441 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     4501 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     4561 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     4621 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     4681 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     4741 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     4801 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     4861 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     4921 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     4981 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     5041 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     5101 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     5161 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     5221 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     5281 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     5341 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     5401 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     5461 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     5521 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     5581 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     5641 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     5701 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     5761 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     5821 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     5881 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     5941 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     6001 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct
     6061 taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac
     6121 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg
     6181 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat
     6241 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct
     6301 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     6361 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     6421 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     6481 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     6541 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     6601 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     6661 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     6721 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     6781 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     6841 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     6901 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     6961 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     7021 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     7081 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     7141 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     7201 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     7261 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     7321 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     7381 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     7441 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     7501 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     7561 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     7621 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     7681 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     7741 ttcagaccca cctcccaacc ccgaggggac ccttgcgcct tttccaaggc agccctgggt
     7801 ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg
     7861 ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg
     7921 tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg
     7981 cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc gcagacggac
     8041 agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat agcggctgct
     8101 cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg gggtgtgggg
     8161 cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag cctccggagc
     8221 gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc cagggggtac
     8281 caccatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag caacggctac
     8341 aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc tctctagcga
     8401 cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt gtgcagaact
     8461 cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat
     8521 cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg tgcttctcga
     8581 tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga cggcagttgg
     8641 gattcgtgaa ttgctgccct ctggttatgt gtgggagggc ctgcagctgc agtagtaaga
     8701 attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     8761 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     8821 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     8881 agatccactt tggcgccggc tcgagggggc ccggggttat taatagtaat caattacggg
     8941 gtcattagtt catagcccat atatggagtt ccg
//
