LOCUS       sequence-466061-        9424 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dual-color reporter express eGFP constitutively ; tdTomato 
            expression is contingent on the inclusion of the frame-shifting 
            cassette exon 7; expresses SMN1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9424)
  AUTHORS   Recinos Y, Ustianenko D, Yeh YT, Wang X, Jacko M, Yesantharao LV, Wu
            Q, Zhang C
  TITLE     CRISPR-dCas13d-based deep screening of proximal and distal 
            splicing-regulatory elements.
  JOURNAL   Nat Commun. 2024 May 7;15(1):3839. doi: 10.1038/s41467-024-47140-8.
  PUBMED    38714659
REFERENCE   2  (bases 1 to 9424)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-05-7"; volume: "15(1):3839. doi"; pages: " 
            10.1038/s41467-024-47140-8"
COMMENT     Sequence Label: pDual-IN-SMN1
FEATURES             Location/Qualifiers
     source          1..9424
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..658
                     /label=chicken beta-actin promoter
     intron          659..1675
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1599..1621
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1683..1702
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             join(1736..1738,1739..1741,1742..2254)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(1781..1802)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2042..2061)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2389..2410
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(2446..3020,3021..3023,3024..3032)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2613..2630)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2840..2859
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3063..3128
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3869..5293
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="SKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGLV
                     TVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQA
                     LKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHL
                     FLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNF
                     EDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLK
                     GEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERS
                     EGRHHLFLYGMDELYK"
     intron          5316..5448
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     5431..5450
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    5457..6045
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5510..5530)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5928..5939)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6072..6089)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6078..6189
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6285..6305)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6285..6303)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6324..6340)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6442..6461)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6507..6562
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6561..6580)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6923..6939)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6947..6963
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6971..6977,6978..6995,6996..7001))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7016..7037
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7096..7291
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7142..7277
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7297..7431
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7334..7353)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7388..7407
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7499..7516)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7670..8258)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7750..7769)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8429..9220,9221..9289))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9052..9071
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9290..9394)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg ggsgcgcgcc aggcggggcg gggcggggcg aggggcgggg
      541 cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc
      601 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg
      661 agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag
      841 ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt
      901 gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg
     1021 cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg
     1081 cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc
     1141 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg
     1201 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
     1261 gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg
     1321 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca
     1381 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc
     1441 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     1501 ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg gctgtccgcg
     1561 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
     1621 cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct
     1681 gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattcg gtaccatggt
     1741 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     1801 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     1861 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     1921 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     1981 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     2041 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     2101 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     2161 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     2221 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     2281 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     2341 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     2401 ggagttcgtg accgccgccg ggatccaccg gatctagaaa ttccgcccct ctccctcccc
     2461 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat
     2521 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt
     2581 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt
     2641 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc
     2701 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc
     2761 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat
     2821 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc
     2881 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
     2941 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
     3001 ttgaaaaaca cgatgataat atggccacaa ccatggctag cataattccc ccaccacctc
     3061 ccgactacaa agaccatgac ggtgattata aagatcatga catcgattac aaggatgacg
     3121 atgacaaggg ctatcatact ggctattata tggtaagtaa tcactcagca tcttttcctg
     3181 acaatttttt tgtagttatg tgactttgtt ttgctgatca tattttgttg aataaaataa
     3241 gtaaaatgtc ttgtgaaaca aaatactttt taacatccat ataaagctat ctatatatag
     3301 ctatctatgt ctatatagct atttttttta acttccttta ttttccttac agggtttcag
     3361 acaaaatcaa aaagaaggaa ggtgctcagc attccttaaa ttaaggagta agtctgccag
     3421 cattatgaaa gtgaatctta cttttgtaaa actttatggt ttgtggaaaa caaatgtttt
     3481 tgaacattta aaaagttcag atgttaaaaa gttgaaaggt taatgtaaaa caatcaatat
     3541 taaagaattt tgatgccaaa actattagat aaaaggttaa tctagatccc tactagaatt
     3601 ctcatactta actggttggt tatgtggaag aaacatactt tcacaataaa gagctttagg
     3661 atatgatgcc attttatatc actagtaggc agaccagcag actttttttt attgtgatat
     3721 gggataacct aggcatactg cactgtacac tctgacatat gaagtgctct agtcaagttt
     3781 aactggtgtc cacagaggac atggtttaac tggaattcgt caagcctctg gttctaattt
     3841 ctcatttgca ggagaaatgc tggcagcgag caagggcgag gaggtcatca aagagttcat
     3901 gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga tcgagggcga
     3961 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg
     4021 ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta
     4081 cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa
     4141 gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc
     4201 cctgcaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga
     4261 cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc
     4321 ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca
     4381 ctacctggtg gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta
     4441 ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga
     4501 acagtacgag cgctccgagg gccgccacca cctgttcctg gggcatggca ccggcagcac
     4561 cggcagcggc agctccggca ccgcctcctc cgaggacaac aacatggccg tcatcaaaga
     4621 gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga
     4681 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     4741 gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa
     4801 ggcgtacgtg aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg
     4861 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga
     4921 ctcctccctg caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc
     4981 ccccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct
     5041 gtacccccgc gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg
     5101 cggccactac ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc
     5161 cggctactac tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat
     5221 cgtggaacag tacgagcgct ccgagggccg ccaccacctg ttcctgtacg gcatggacga
     5281 gctgtacaag tagcttaagt aactcgaggc cgcaggtaag tatcaaggtt acaagacagg
     5341 tttaaggaga ccaatagaaa ctgggcttgt cgagacagag aagactcttg cgtttctgat
     5401 aggcacctat tggtcttact gacatccact ttgcctttct ctccacaggt gtcgacaatc
     5461 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     5521 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     5581 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     5641 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     5701 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     5761 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     5821 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     5881 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     5941 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     6001 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga attcgagctc
     6061 ggtacgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     6121 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     6181 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     6241 cagcaagggg gaggattggg aagacaatag cccagctttt gttcccttta gtgagggtta
     6301 attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgcta
     6361 attcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct
     6421 ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa
     6481 gccccttgag catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg
     6541 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca
     6601 tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa
     6661 caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt
     6721 attccataga aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt
     6781 tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct
     6841 ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca
     6901 gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     6961 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     7021 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     7081 cgtgccagcg gatccgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     7141 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     7201 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     7261 aggctttttt ggaggcctag gcttttgcaa aaagctaact tgtttattgc agcttataat
     7321 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     7381 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ccgctgcatt
     7441 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     7501 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     7561 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     7621 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     7681 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     7741 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     7801 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     7861 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     7921 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     7981 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     8041 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     8101 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     8161 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     8221 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     8281 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     8341 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     8401 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     8461 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     8521 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     8581 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     8641 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     8701 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     8761 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     8821 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     8881 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     8941 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     9001 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     9061 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     9121 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     9181 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     9241 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     9301 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     9361 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     9421 ggtc
//
