LOCUS       sequence-466069-        9855 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A CMV driven human TCF7L2 followed by a P2A cleavage peptide and an 
            H2A mCherry fusion protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9855)
  AUTHORS   Cantu Gutierrez ME, Hill MC, Largoza G, Martin JF, Wythe JD
  TITLE     Defining the Transcriptional and Epigenetic Basis of Organotypic 
            Endothelial Diversity in the Developing and Adult Mouse
  JOURNAL   bioRxiv 2021.11.15.468651
REFERENCE   2  (bases 1 to 9855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.11.15.468651"
COMMENT     Sequence Label: JDW 1045 (pLenti6.2_CMV_V5_TCF7L2_P2A_H2A_mChe...
FEATURES             Location/Qualifiers
     source          1..9855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    171..296
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             343..523
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(524..750)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(778..798)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(778..796)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(817..833)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(817..833)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(830..852)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    841..857
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(865..871,872..889,890..895))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    910..931
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1048..1065)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1219..1807)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1299..1318)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1978..2769,2770..2838))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2601..2620
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2839..2943)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2969..3424)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3106..3127)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3318..3337
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3551..3573
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3565..3582
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3566..3582
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3592..3611
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3592..3610
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      3631..3766
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3806..3927
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3817..3836)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3871..3890
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             3999..4232
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(4319..4717)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(4736..4783)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(4797..5296)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4845..4864)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     5238..5261
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             complement(5329..5370)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    5423..5447
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(5465..6175)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5545..5568)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(5743..5762)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6012..6030
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(6572..6628)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(8021..8062)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     regulatory      8062..8071
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(8078..8102)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(8198..8222)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(8219..8422)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8248..8268)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8423..8726
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(9071..9115)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9300..9533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 cccagtattt gtctacagcc ttctgatgtt tctaacaggc caggattaac tgcgaatcgt
       61 tctagctccc tgcttgccca tactatatgt tttaatttat attttttctt tccccctggc
      121 cttaaccgaa ttttttccca tcgcgatcta attctccccc gcttaatact gacgctctcg
      181 cacccatctc tctccttcta gcctccgcta gtcaaaattt ttggcgtact caccagtcgc
      241 cgcccctcgc ctcttgccgt gcgcgcttca gcaagccgag tcctgcgtcg agagagctcc
      301 tctggtttcc ctttcgcttt caagtccctg ttcgggcgcc actgctagag attttccaca
      361 ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga cgggcacaca
      421 ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc cctagttagc
      481 cagagagctc ccaggctcag atctggtcta accagagaga cccgtttatg tatcgagcta
      541 ggcacttaaa tacaatatct ctgcaatgcg gcaattcagt ggttcgtcca atccatgtca
      601 gacccgtctg ttgccttcct aataaggcac gatcgtacca ccttacttcc accaatcggc
      661 atgcacggtg ctttttctct ccttgtaagg catgttgcta actcatcgtt accatgttgc
      721 aagactacaa gagtattgca taagactaca ttaagcttgc agctccagct tttgttccct
      781 ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
      841 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
      901 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
      961 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     1021 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     1081 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     1141 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     1201 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     1261 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     1321 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     1381 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     1441 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     1501 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     1561 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     1621 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     1681 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     1741 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     1801 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     1861 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     1921 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     1981 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     2041 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     2101 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     2161 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     2221 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     2281 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     2341 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     2401 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     2461 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     2521 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     2581 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     2641 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     2701 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     2761 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     2821 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     2881 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     2941 gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc
     3001 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     3061 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     3121 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     3181 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     3241 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     3301 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     3361 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     3421 gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     3481 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     3541 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac
     3601 tcactatagg gcgaattggg tacgtcccta ggcctccaaa aaagcctcct cactacttct
     3661 ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc
     3721 catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg
     3781 gcgggatagc tagagccaga catgataaga tacattgatg agtttggaca aaccacaact
     3841 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     3901 accattataa gctgcaataa acaagttcct ctcactctct gatattcatt tctttgcaag
     3961 ttataaatac tgaataataa gatgacatga actactactg ctagagattt tccacactga
     4021 ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac
     4081 ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga
     4141 gagctcccag gctcagatct ggtctaacca gagagaccca gtacaagcaa aaagcagatc
     4201 ttgtcttcgt tgggagtgaa ttagcccttc cagtcccccc ttttctttta aaaagtggct
     4261 aagatctaca gctgccttgt aagtcattgg tcttaaaggt accgagctcg aattgtgctt
     4321 agccctccca cacataacca gagggcagca attcacgaat cccaactgcc gtcggctgtc
     4381 catcactgtc cttcactatg gctttgatcc caggatgcag atcgagaagc acctgtcggc
     4441 accgtccgca ggggctcaag atgcccctgt tctcatttcc gatcgcgacg atacaagtca
     4501 ggttgccagc tgccgcagca gcagcagtgc ccagcaccac gagttctgca caaggtcccc
     4561 cagtaaaatg atatacattg acaccagtga agatgcggcc gtcgctagag agagctgcgc
     4621 tggcgacgct gtagtcttca gagatgggga tgctgttgat tgtagccgtt gctctttcaa
     4681 tgagggtgga ttcttcttga gacaaaggct tggccatggt ttagttcctc accttgtcgt
     4741 attatactat gccgatatac tatgccgatg attaattgtc aacacgtgtc tagagtcgaa
     4801 aggcccggag atgaggaaga ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag
     4861 aatgccgggc ctccggagga ccttcgggcg cccgccccgc ccctgagccc gcccctgagc
     4921 ccgcccccgg acccacccct tcccagcctc tgagcccaga aagcgaagga gcaaagctgc
     4981 tattggccgc tgccccaaag gcctacccgc ttccattgct cagcggtgct gtccatctgc
     5041 acgagactag tgagacgtgc tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg
     5101 gcggggggga acttcctgac taggggagga gtagaaggtg gcgcgaaggg gccaccaaag
     5161 aacggagccg gttggcgcct accggtggat gtggaatgtg tgcgaggcca gaggccactt
     5221 gtgtagcgcc aagtgcccag cggggctgct aaagcgcatg ctccagactg ccttgggaaa
     5281 agcgcctccc ctacccggta ggtaccatcg tcattactaa ccggtacgcg tagaatcgag
     5341 accgaggaga gggttaggga taggcttacc ttcgaaccgc gggccctcta gactcgagcg
     5401 gccgccactg tgctggatat caaccacttt gtacaagaaa gctgggagat ctctcgagct
     5461 atcattactt gtacagctcg tccatgccgc cggtggagtg gcggccctcg gcgcgctcgt
     5521 actgttccac gatggtgtag tcctcgttgt gggaggtgat gtccagcttg atgttgacgt
     5581 tgtaggcgcc gggcagctgc acgggcttct tggccttgta ggtggtcttg acctcggcgt
     5641 cgtagtggcc gccgtccttc agcttcagcc tctgcttgat ctcgcccttc agggcgccgt
     5701 cctcggggta catccgctcg gaggaggcct cccagcccat ggtcttcttc tgcattacgg
     5761 ggccgtcgga ggggaagttg gtgccgcgca gcttcacctt gtagatgaac tcgccgtcct
     5821 gcagggagga gtcctgggtc acggtcacca cgccgccgtc ctcgaagttc atcacgcgct
     5881 cccacttgaa gccctcgggg aaggacagct tcaagtagtc ggggatgtcg gcggggtgct
     5941 tcacgtaggc cttggagccg tacatgaact gaggggacag gatgtcccag gcgaagggca
     6001 gggggccgcc cttggtcacc ttcagcttgg cggtctgggt gccctcgtag gggcggccct
     6061 cgccctcgcc ctcgatctcg aactcgtggc cgttcacgga gccctccatg tgcaccttga
     6121 agcgcatgaa ctccttgatg atggccatgt tgtcctcctc gcccttgctc accatccccc
     6181 ctgcggtttt ctgctggccc ttcttaccaa tcagagactt gtggatgtga ggaatgactc
     6241 ctcctccagc aatggtggcc ttgataagcg agtccagctc ctcgtctccg cggatggcca
     6301 gctgtaaatg tctgggagtg attcgcttca ccttcagatc cttagaagcg tttccagcca
     6361 actccagcac ctcagcggtg agatactcca ggatagcagc actgtaaacc gcagctgtag
     6421 ctccgacacg gccatggctg gtggttcgtg tcttcaggtg cctgtggata cgacctacag
     6481 ggaactgcag accagctctc tgagatctgg agacggcttt cgtcttggct tttccactgt
     6541 ctttacctgc ttttccacct gccatcctag gtggcccggg attctcttcg acatcccctg
     6601 cttgtttcaa cagggagaag ttagtggctc cggtaccgca gtaagcttcc atctgaagag
     6661 ggtgggctga ggcagctgcc ttcaccttgt atgtagcgaa cgcacttttt ttttctcctg
     6721 taatcggagg aagtgaaagg caaggattta ggaaacattc gctgtgttca ttggtctctc
     6781 ccggctgctt gtcccttttc ctcttcttct tctttccata gttatcccgc gcggaccagc
     6841 cggggtacag ttgcatatga agctgtcgct ccttccgggc cagctcgtag tatttcgctt
     6901 gctcttctct ggacagtgca tgccacctcc gcccaaggat ctggttgatg gccgcgcttt
     6961 ctttcaacgt gcactcagct acgacctttg ctctcatttc cttcatatac aacatgaatg
     7021 cattaagagg tttctttatg tggggcttct tcttttcttc ttcctttttg gagtcctgat
     7081 gctttgaact atggagtgag ccgacatcac tctgggacga ttcctgtttg actgttggtg
     7141 tgactatggc cggatgcgga atgcccgtcg tgtgtagcgt atgatgtggt gggaccatat
     7201 ggggagggaa cctggacatg gaagcattga cggtcagagc tgtggggtag gggtgtctga
     7261 atcctcctgt cgtgattggg tacactggtt gaccttgcca tcctagcgga tgggggattt
     7321 gtcctacggt gccaggcgat agtgggtaat acggggatat atctggaggg tgcggaggcc
     7381 gtgggattcc tgttttgggg tctacgtcgg ctggtaagtg tggaggtggg tttcccggcg
     7441 tgaagtgttc attgctgtac gtgataagag gcgtgagggg gtggacatgg tgagggtgct
     7501 gcaccactgg cactttgtta gagacaatgt gtgccggtga tggggaccgg gcatccttga
     7561 gggcttgtct actctggagg ctccctgcct ggacatcaag cagtggccat ttcatctgga
     7621 gataggttcg ggcggtgggc gagagcgatc cgttggggag gtaggggctc gtcaggtcgg
     7681 ggatcatgat gaaggggtag ccgggatacg gtggcccctt aaagagccct ccatcttgcc
     7741 tcttggccgc ttcttccaaa ctttcccggg atttgtctcg gaaactttcg gagcgaggcg
     7801 gaggccgtct ttccgcctcg gaatcggagg agctgttttg attcgtttct gattcattga
     7861 ctagagacga tttgacatca gctaaatccc tctctgccga ggagttttcg gagctcttct
     7921 cctcctgttc gccctcgtct ttgaaggaaa tcagttcgtc gttggcgcct aggtcatccc
     7981 ctccaccgcc gttcagctgc ggcataccac cagcaccacc cgtagaatcg agaccgagga
     8041 gagggttagg gataggctta cccatggtgg cggatccagc ctgctttttt gtacaaactt
     8101 gttgatatct gcagaattcc accacactgg actagtggat cctctagagt cggtgtcttc
     8161 tatggaggtc aaaacagcgt ggatggcgtc tccaggcgat ctgacggttc actaaacgag
     8221 ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg
     8281 agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga
     8341 cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga
     8401 tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc
     8461 aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt
     8521 cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg
     8581 cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta
     8641 tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg
     8701 ggccatttac cgtaagttat gtaacgcgga actcccaagc ttatcgcaga cccagtcgct
     8761 agcgttaacg tcgacatcgc agacccagtc gctagcgtta acgtcgacat cgataccgtc
     8821 gagatccgtt cactaatcga atggatctgt ctctgtctct ctctccacct tcttcttcta
     8881 ttccttcggg cctgtcgggt cccctcgggg ttgggaggtg ggtctgaaac gataatggtg
     8941 aatatccctg cctaactcta ttcactatag aaagtacagc aaaaactatt cttaaaccta
     9001 ccaagcctcc tactatcatt atgaataatt ttatatacca cagccaattt gttatgttaa
     9061 accaattcca caaacttgcc catttatcta attccaataa ttcttgttca ttcttttctt
     9121 gctggttttg cgattcttca attaaggagt gtattaagct tgtgtaattg ttaatttctc
     9181 tgtcccactc catccaggtc gtgtgattcc aaatctgttc cagagattta ttactccaac
     9241 tagcattcca aggcacagca gtggtgcaaa tgagttttcc agagcaaccc caaatcccca
     9301 ggagctgttg atcctttagg tatctttcca cagccaggat tcttgcctgg agctgcttga
     9361 tgccccagac tgtgagttgc aacagatgct gttgcgcctc aatagccctc agcaaattgt
     9421 tctgctgctg cactatacca gacaataatt gtctggcctg taccgtcagc gtcattgacg
     9481 ctgcgcccat agtgcttcct gctgctccca agaacccaag gaacaaagct cctattccca
     9541 ctgctctttt ttctctctgc accactcttc tctttgcctt ggtgggtgct actcctaatg
     9601 gttcaatttt tactacttta tatttatata attcacttct ccaattgtcc ctcatatctc
     9661 ctcctccagg tctgaagatc agcggccgct tgctgtgcgg tggtcttact tttgttttgc
     9721 tcttcctcta tcttgtctaa agcttccttg gtgtctttta tctctatcct ttgatgcaca
     9781 caatagaggg ttgctactgt attatataat gatctaagtt cttctgatcc tgtctgaagg
     9841 gatggttgta gctgt
//
