LOCUS       sequence-466070-        9642 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A CMV driven human TCF7 followed by a P2A cleavage peptide and an 
            H2A mCherry fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9642)
  AUTHORS   Cantu Gutierrez ME, Hill MC, Largoza G, Martin JF, Wythe JD
  TITLE     Defining the Transcriptional and Epigenetic Basis of Organotypic 
            Endothelial Diversity in the Developing and Adult Mouse
  JOURNAL   bioRxiv 2021.11.15.468651
REFERENCE   2  (bases 1 to 9642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.11.15.468651"
COMMENT     Sequence Label: JDW 1039 (pLenti6.2_CMV_V5_TCF7_P2A_H2A_mCherry)
FEATURES             Location/Qualifiers
     source          1..9642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    87..320
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    813..938
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             985..1165
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1166..1392)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1420..1440)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1420..1438)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1459..1475)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1459..1475)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1472..1494)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1483..1499
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1507..1513,1514..1531,1532..1537))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1552..1573
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1690..1707)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1861..2449)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1941..1960)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2620..3411,3412..3480))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3243..3262
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3481..3585)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3611..4066)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3748..3769)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3960..3979
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4193..4215
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4207..4224
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4208..4224
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4234..4253
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4234..4252
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      4273..4408
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4448..4569
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4459..4478)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4513..4532
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4641..4874
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(4961..5359)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(5378..5425)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(5439..5938)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5487..5506)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     5880..5903
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             complement(5971..6012)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    6065..6089
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(6107..6817)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6187..6210)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(6385..6404)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6654..6672
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(7214..7270)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(8450..8491)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     regulatory      8491..8500
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(8507..8531)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(8627..8651)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(8648..8851)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8677..8697)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8852..9155
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(9500..9544)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tctgttccag agatttatta ctccaactag cattccaagg cacagcagtg gtgcaaatga
       61 gttttccaga gcaaccccaa atccccagga gctgttgatc ctttaggtat ctttccacag
      121 ccaggattct tgcctggagc tgcttgatgc cccagactgt gagttgcaac agatgctgtt
      181 gcgcctcaat agccctcagc aaattgttct gctgctgcac tataccagac aataattgtc
      241 tggcctgtac cgtcagcgtc attgacgctg cgcccatagt gcttcctgct gctcccaaga
      301 acccaaggaa caaagctcct attcccactg ctcttttttc tctctgcacc actcttctct
      361 ttgccttggt gggtgctact cctaatggtt caatttttac tactttatat ttatataatt
      421 cacttctcca attgtccctc atatctcctc ctccaggtct gaagatcagc ggccgcttgc
      481 tgtgcggtgg tcttactttt gttttgctct tcctctatct tgtctaaagc ttccttggtg
      541 tcttttatct ctatcctttg atgcacacaa tagagggttg ctactgtatt atataatgat
      601 ctaagttctt ctgatcctgt ctgaagggat ggttgtagct gtcccagtat ttgtctacag
      661 ccttctgatg tttctaacag gccaggatta actgcgaatc gttctagctc cctgcttgcc
      721 catactatat gttttaattt atattttttc tttccccctg gccttaaccg aattttttcc
      781 catcgcgatc taattctccc ccgcttaata ctgacgctct cgcacccatc tctctccttc
      841 tagcctccgc tagtcaaaat ttttggcgta ctcaccagtc gccgcccctc gcctcttgcc
      901 gtgcgcgctt cagcaagccg agtcctgcgt cgagagagct cctctggttt ccctttcgct
      961 ttcaagtccc tgttcgggcg ccactgctag agattttcca cactgactaa aagggtctga
     1021 gggatctcta gttaccagag tcacacaaca gacgggcaca cactacttga agcactcaag
     1081 gcaagcttta ttgaggctta agcagtgggt tccctagtta gccagagagc tcccaggctc
     1141 agatctggtc taaccagaga gacccgttta tgtatcgagc taggcactta aatacaatat
     1201 ctctgcaatg cggcaattca gtggttcgtc caatccatgt cagacccgtc tgttgccttc
     1261 ctaataaggc acgatcgtac caccttactt ccaccaatcg gcatgcacgg tgctttttct
     1321 ctccttgtaa ggcatgttgc taactcatcg ttaccatgtt gcaagactac aagagtattg
     1381 cataagacta cattaagctt gcagctccag cttttgttcc ctttagtgag ggttaattgc
     1441 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     1501 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     1561 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     1621 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     1681 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     1741 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     1801 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1861 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     1921 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1981 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     2041 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     2101 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     2161 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     2221 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     2281 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     2341 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     2401 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     2461 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     2521 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     2581 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     2641 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     2701 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     2761 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     2821 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     2881 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     2941 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     3001 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     3061 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     3121 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     3181 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     3241 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     3301 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     3361 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     3421 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     3481 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     3541 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     3601 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     3661 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     3721 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     3781 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     3841 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     3901 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     3961 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     4021 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
     4081 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     4141 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     4201 cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg
     4261 ggtacgtccc taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggccg
     4321 aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg
     4381 cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggata gctagagcca
     4441 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     4501 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     4561 aaacaagttc ctctcactct ctgatattca tttctttgca agttataaat actgaataat
     4621 aagatgacat gaactactac tgctagagat tttccacact gactaaaagg gtctgaggga
     4681 tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa
     4741 gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat
     4801 ctggtctaac cagagagacc cagtacaagc aaaaagcaga tcttgtcttc gttgggagtg
     4861 aattagccct tccagtcccc ccttttcttt taaaaagtgg ctaagatcta cagctgcctt
     4921 gtaagtcatt ggtcttaaag gtaccgagct cgaattgtgc ttagccctcc cacacataac
     4981 cagagggcag caattcacga atcccaactg ccgtcggctg tccatcactg tccttcacta
     5041 tggctttgat cccaggatgc agatcgagaa gcacctgtcg gcaccgtccg caggggctca
     5101 agatgcccct gttctcattt ccgatcgcga cgatacaagt caggttgcca gctgccgcag
     5161 cagcagcagt gcccagcacc acgagttctg cacaaggtcc cccagtaaaa tgatatacat
     5221 tgacaccagt gaagatgcgg ccgtcgctag agagagctgc gctggcgacg ctgtagtctt
     5281 cagagatggg gatgctgttg attgtagccg ttgctctttc aatgagggtg gattcttctt
     5341 gagacaaagg cttggccatg gtttagttcc tcaccttgtc gtattatact atgccgatat
     5401 actatgccga tgattaattg tcaacacgtg tctagagtcg aaaggcccgg agatgaggaa
     5461 gaggagaaca gcgcggcaga cgtgcgcttt tgaagcgtgc agaatgccgg gcctccggag
     5521 gaccttcggg cgcccgcccc gcccctgagc ccgcccctga gcccgccccc ggacccaccc
     5581 cttcccagcc tctgagccca gaaagcgaag gagcaaagct gctattggcc gctgccccaa
     5641 aggcctaccc gcttccattg ctcagcggtg ctgtccatct gcacgagact agtgagacgt
     5701 gctacttcca tttgtcacgt cctgcacgac gcgagctgcg gggcgggggg gaacttcctg
     5761 actaggggag gagtagaagg tggcgcgaag gggccaccaa agaacggagc cggttggcgc
     5821 ctaccggtgg atgtggaatg tgtgcgaggc cagaggccac ttgtgtagcg ccaagtgccc
     5881 agcggggctg ctaaagcgca tgctccagac tgccttggga aaagcgcctc ccctacccgg
     5941 taggtaccat cgtcattact aaccggtacg cgtagaatcg agaccgagga gagggttagg
     6001 gataggctta ccttcgaacc gcgggccctc tagactcgag cggccgccac tgtgctggat
     6061 atcaaccact ttgtacaaga aagctgggag atctctcgag ctatcattac ttgtacagct
     6121 cgtccatgcc gccggtggag tggcggccct cggcgcgctc gtactgttcc acgatggtgt
     6181 agtcctcgtt gtgggaggtg atgtccagct tgatgttgac gttgtaggcg ccgggcagct
     6241 gcacgggctt cttggccttg taggtggtct tgacctcggc gtcgtagtgg ccgccgtcct
     6301 tcagcttcag cctctgcttg atctcgccct tcagggcgcc gtcctcgggg tacatccgct
     6361 cggaggaggc ctcccagccc atggtcttct tctgcattac ggggccgtcg gaggggaagt
     6421 tggtgccgcg cagcttcacc ttgtagatga actcgccgtc ctgcagggag gagtcctggg
     6481 tcacggtcac cacgccgccg tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg
     6541 ggaaggacag cttcaagtag tcggggatgt cggcggggtg cttcacgtag gccttggagc
     6601 cgtacatgaa ctgaggggac aggatgtccc aggcgaaggg cagggggccg cccttggtca
     6661 ccttcagctt ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct
     6721 cgaactcgtg gccgttcacg gagccctcca tgtgcacctt gaagcgcatg aactccttga
     6781 tgatggccat gttgtcctcc tcgcccttgc tcaccatccc ccctgcggtt ttctgctggc
     6841 ccttcttacc aatcagagac ttgtggatgt gaggaatgac tcctcctcca gcaatggtgg
     6901 ccttgataag cgagtccagc tcctcgtctc cgcggatggc cagctgtaaa tgtctgggag
     6961 tgattcgctt caccttcaga tccttagaag cgtttccagc caactccagc acctcagcgg
     7021 tgagatactc caggatagca gcactgtaaa ccgcagctgt agctccgaca cggccatggc
     7081 tggtggttcg tgtcttcagg tgcctgtgga tacgacctac agggaactgc agaccagctc
     7141 tctgagatct ggagacggct ttcgtcttgg cttttccact gtctttacct gcttttccac
     7201 ctgccatcct aggtggcccg ggattctctt cgacatcccc tgcttgtttc aacagggaga
     7261 agttagtggc tccggtaccc aagagcactg tcatcggaag gaacggggct ggggcagcgg
     7321 ccttctccgg gtaagtaccg aatgcatttc tttttcctcc tgtggtggat tcttggtgct
     7381 tttccctcga ccgcctcttc ttcttcccgt agttgtcccg cgctgaccag cctgggtata
     7441 gctgcatgtg cagctgcctc tccttgcggg ccagctcata gtacttggcc tgctcttctc
     7501 gcgacagcgc gtgccacctg cggcccagga tctggttgat ggcagcgctc tccttaagtg
     7561 tgcactctgc aatgaccttg gctctcatct ccttcatgta cagcatgaag gcattgaggg
     7621 gcttcttgat ggttggcttc ttggcctcct tctctgcctt ggactctgct tgtgtcttca
     7681 ggttgcggtc gaagggctgc agctcctgct tccctgaggg gggcacaatg gccgggtggg
     7741 ggatggctgc tgggtgacca ggtacaccag aacctagcat caaggatggg tgggtgaacc
     7801 agctcacagt gtgggggagc tgccccatgc tgcctgaggt cagggagtag aagccagaga
     7861 ggtcaggggt ctgcagaggc ctgtgaactt gcttctggct gatgtccgca ggtgcagggg
     7921 tgggatgtgg gctgttgaaa tgttcgtaga gagagagttg ggggacaccg tgggggggct
     7981 gattggcctt gtgcagcggg ggctgcggct gggggtgctg ccctgctccc gaggggggtg
     8041 ggtaatgcat gagcagattg aaggcggagt agacggtctc tttgtacatg ccgctggtgc
     8101 actccggggc cttcaggccg tcctccaggg gctctggaag tttgtccggg aagagtctct
     8161 gcgcagcgtg ttcccgcccg agagcctcgg cctcgccgcg ggccccggcg ccggcccccg
     8221 ggacccccgg gatccctgcg ccgccggccg cgccctcgga ctcgttcacg agcgacgact
     8281 tgagctcggc caggtcgcgc tcgggaccgg cggcgctgtc gcggctcttg tcgtcctgct
     8341 cctcgccttc atcctggaag gccagcagct cgtccggcgc gccgaggtcg tcgccgccgc
     8401 ccgcgccgcc cccgccggag tccagctgcg gcataccacc agcaccaccc gtagaatcga
     8461 gaccgaggag agggttaggg ataggcttac ccatggtggc agatccagcc tgcttttttg
     8521 tacaaacttg ttgatatctg cagaattcca ccacactgga ctagtggatc ctctagagtc
     8581 ggtgtcttct atggaggtca aaacagcgtg gatggcgtct ccaggcgatc tgacggttca
     8641 ctaaacgagc tctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca
     8701 atggggcgga gttgttacga cattttggaa agtcccgttg attttggtgc caaaacaaac
     8761 tcccattgac gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgctatccac
     8821 gcccattgat gtactgccaa aaccgcatca ccatggtaat agcgatgact aatacgtaga
     8881 tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc
     8941 atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg
     9001 ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg
     9061 gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc
     9121 agccaggcgg gccatttacc gtaagttatg taacgcggaa ctcccaagct tatcgcagac
     9181 ccagtcgcta gcgttaacgt cgacatcgca gacccagtcg ctagcgttaa cgtcgacatc
     9241 gataccgtcg agatccgttc actaatcgaa tggatctgtc tctgtctctc tctccacctt
     9301 cttcttctat tccttcgggc ctgtcgggtc ccctcggggt tgggaggtgg gtctgaaacg
     9361 ataatggtga atatccctgc ctaactctat tcactataga aagtacagca aaaactattc
     9421 ttaaacctac caagcctcct actatcatta tgaataattt tatataccac agccaatttg
     9481 ttatgttaaa ccaattccac aaacttgccc atttatctaa ttccaataat tcttgttcat
     9541 tcttttcttg ctggttttgc gattcttcaa ttaaggagtg tattaagctt gtgtaattgt
     9601 taatttctct gtcccactcc atccaggtcg tgtgattcca aa
//
