LOCUS       sequence-466071-       10241 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A CMV driven human TCF7L1 followed by a P2A cleavage peptide and an 
            H2A mCherry fusion protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10241)
  AUTHORS   Cantu Gutierrez ME, Hill MC, Largoza G, Martin JF, Wythe JD
  TITLE     Defining the Transcriptional and Epigenetic Basis of Organotypic 
            Endothelial Diversity in the Developing and Adult Mouse
  JOURNAL   bioRxiv 2021.11.15.468651
REFERENCE   2  (bases 1 to 10241)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.11.15.468651"
COMMENT     Sequence Label: JDW 1042 (pLenti6.2_CMV_V5_TCF7L1_P2A_H2A_mChe...
FEATURES             Location/Qualifiers
     source          1..10241
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(15..39)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             92..133
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     promoter        166..665
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(201..224)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     598..617
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        679..726
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             745..1143
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             1230..1463
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1535..1656
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1572..1591)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1626..1645
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1696..1831
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1851..1870)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1852..1870)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1880..1897)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1880..1896)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1889..1911)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2038..2493
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2125..2144)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2335..2356
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2519..2623
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2624..2692,2693..3484)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2842..2861)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3655..4243
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4144..4163
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4397..4414
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4531..4552
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4567..4572,4573..4590,4591..4597)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4605..4621
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4610..4632
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4629..4645
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4629..4645
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4664..4684
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4666..4684
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4712..4938
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4939..5119
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5166..5291
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5784..6017
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6202..6246
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        6591..6894
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6895..7098
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7049..7069
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7095..7119
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    7215..7239
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      7245..7254
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7254..7295
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             9075..9131
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             9528..10238
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(9673..9691)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     9941..9960
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     10135..10158
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 tagctcgaga gatctcccag ctttcttgta caaagtggtt gatatccagc acagtggcgg
       61 ccgctcgagt ctagagggcc cgcggttcga aggtaagcct atccctaacc ctctcctcgg
      121 tctcgattct acgcgtaccg gttagtaatg acgatggtac ctaccgggta ggggaggcgc
      181 ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca
      241 caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt
      301 tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg
      361 ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg
      421 tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat
      481 agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg
      541 ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat
      601 tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc
      661 tttcgactct agacacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa
      721 tacgacaagg tgaggaacta aaccatggcc aagcctttgt ctcaagaaga atccaccctc
      781 attgaaagag caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc
      841 agcgcagctc tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact
      901 gggggacctt gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac
      961 ctgacttgta tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg
     1021 tgccgacagg tgcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat
     1081 ggacagccga cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc
     1141 taagcacaat tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct
     1201 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     1261 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     1321 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     1381 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     1441 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     1501 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     1561 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     1621 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     1681 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     1741 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     1801 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
     1861 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     1921 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     1981 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
     2041 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     2101 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     2161 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     2221 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     2281 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     2341 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     2401 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     2461 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     2521 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     2581 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     2641 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     2701 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     2761 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     2821 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     2881 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     2941 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     3001 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     3061 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     3121 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     3181 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     3241 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     3301 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     3361 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     3421 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     3481 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     3541 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     3601 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     3661 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     3721 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     3781 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     3841 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     3901 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     3961 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     4021 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     4081 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     4141 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     4201 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     4261 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     4321 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     4381 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     4441 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     4501 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     4561 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     4621 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
     4681 aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct
     4741 tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca
     4801 tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt
     4861 ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc
     4921 ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     4981 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     5041 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     5101 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     5161 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     5221 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     5281 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     5341 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     5401 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     5461 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     5521 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     5581 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
     5641 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     5701 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     5761 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     5821 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     5881 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     5941 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     6001 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     6061 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     6121 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     6181 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     6241 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     6301 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     6361 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     6421 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
     6481 ttagtgaacg gatctcgacg gtatcgatgt cgacgttaac gctagcgact gggtctgcga
     6541 tgtcgacgtt aacgctagcg actgggtctg cgataagctt gggagttccg cgttacataa
     6601 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     6661 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     6721 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
     6781 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     6841 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
     6901 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
     6961 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
     7021 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
     7081 gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc
     7141 tgttttgacc tccatagaag acaccgactc tagaggatcc actagtccag tgtggtggaa
     7201 ttctgcagat atcaacaagt ttgtacaaaa aagcaggctg gatcgccacc atgggtaagc
     7261 ctatccctaa ccctctcctc ggtctcgatt ctacgggtgg tgctggtggt atgccccagc
     7321 tcggcggcgg gggcggcggc ggcggcggca gcgggggagg cggcggctcc agcgccgggg
     7381 cggccggcgg aggggacgac ctcggggcga acgacgagct gatccccttc caggacgagg
     7441 ggggcgagga gcaggagccg agcagcgata gcgcctcggc gcagcgggac ctagacgagg
     7501 tcaagtcgtc cctggtcaac gagtcggaga accagagcag cagctcggac tcggaggcgg
     7561 agaggcgccc gcagcccgtc cgggacactt tccagaagcc gcgggactat ttcgccgaag
     7621 tgagaaggcc tcaggacagc gcgttcttta aaggaccccc gtaccctggg taccccttcc
     7681 tgatgatccc ggacctgagc agcccgtacc tctccaacgg acccctgtct cccggaggag
     7741 cgcgcaccta cctgcagatg aaatggcccc tcctcgatgt cccctccagc gccacagtca
     7801 aggacacgag gtcaccatct ccagcacact tgtctaataa agttcctgtc gttcagcacc
     7861 cgcatcacat gcatccgctg actcccctca tcacctacag caatgaccac ttctcccccg
     7921 gctcccctcc cacccacctc tccccagaga tcgatccaaa gacaggaatc ccccggcccc
     7981 ctcacccatc cgagctgtca ccgtattacc cactctctcc cggagctgtc ggacaaatcc
     8041 cccaccccct cggctggctc gtcccacagc aaggccagcc catgtactcc cttcctcccg
     8101 gtggcttccg gcacccttac cccgccctcg ccatgaacgc ctcgatgtcc agcctggtct
     8161 ccagtcggtt ctctcctcac atggtggctc ctgcccaccc tggcctgccc acctcaggga
     8221 tcccccaccc tgccatcgtc tcccccatcg tcaagcagga accggcaccc cccagcctga
     8281 gccctgcagt gagcgtgaaa tcaccagtca ccgtgaaaaa ggaggaggaa aagaagcccc
     8341 acgtgaagaa gcctctgaat gccttcatgt tgtatatgaa ggagatgagg gccaaggtgg
     8401 tggctgagtg caccctgaag gaaagtgcag ccattaacca gatccttgga agaaagtggc
     8461 acaacctgtc tcgagaagaa caggccaagt actacgagct ggcccggaag gagcggcagc
     8521 ttcactcgca gctctaccca acctggtcag cccgggacaa ctatggtaag aaaaagaaga
     8581 ggaagagaga aaagcagctg tcccagacac agtcacagca gcaagtccag gaggcagagg
     8641 gtgccctggc ctccaagagc aagaagccat gtgttcagta cctgcccccc gagaagccct
     8701 gtgacagccc tgcctcctcc cacgggagca tgctggactc cccggccact ccctctgcag
     8761 ctttggcctc accagctgcc cctgctgcca cccattcgga gcaagcccag cccctctccc
     8821 tcaccaccaa accagaaacc cgggcccagc tggctctcca ctctgccgcc ttcctgtcgg
     8881 ctaaggctgc agcctcctcc tctaggcaga tgggcagcca gcctcccctc ctgtcccggc
     8941 ccctccccct tgggtccatg cccacagctc tgctggcctc tcccccgtcc ttccccgcca
     9001 cgctccatgc ccaccaggcc ctcccggtgc tacaggccca gcctctttcc ctggtcacca
     9061 agtctgccca cggagccact aacttctccc tgttgaaaca agcaggggat gtcgaagaga
     9121 atcccgggcc acctaggatg gcaggtggaa aagcaggtaa agacagtgga aaagccaaga
     9181 cgaaagccgt ctccagatct cagagagctg gtctgcagtt ccctgtaggt cgtatccaca
     9241 ggcacctgaa gacacgaacc accagccatg gccgtgtcgg agctacagct gcggtttaca
     9301 gtgctgctat cctggagtat ctcaccgctg aggtgctgga gttggctgga aacgcttcta
     9361 aggatctgaa ggtgaagcga atcactccca gacatttaca gctggccatc cgcggagacg
     9421 aggagctgga ctcgcttatc aaggccacca ttgctggagg aggagtcatt cctcacatcc
     9481 acaagtctct gattggtaag aagggccagc agaaaaccgc aggggggatg gtgagcaagg
     9541 gcgaggagga caacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
     9601 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     9661 gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca
     9721 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
     9781 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     9841 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
     9901 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
     9961 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
    10021 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgccgag gtcaagacca
    10081 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagctgg
    10141 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgagcgc gccgagggcc
    10201 gccactccac cggcggcatg gacgagctgt acaagtaatg a
//
