LOCUS       sequence-466073-       10070 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A CMV driven human FOXF1 followed by a P2A cleavage peptide and an 
            H2A mCherry fusion protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10070)
  AUTHORS   Cantu Gutierrez ME, Hill MC, Largoza G, Martin JF, Wythe JD
  TITLE     Defining the Transcriptional and Epigenetic Basis of Organotypic 
            Endothelial Diversity in the Developing and Adult Mouse
  JOURNAL   bioRxiv 2021.11.15.468651
REFERENCE   2  (bases 1 to 10070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.11.15.468651"
COMMENT     Sequence Label: JDW 1392 (pLenti-CMV-FOXF1-P2A-H2A-mCherry)
FEATURES             Location/Qualifiers
     source          1..10070
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(72..116)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    301..534
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1027..1152
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1199..1379
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1380..1606)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1634..1654)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1634..1652)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1673..1689)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1673..1689)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1686..1708)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1697..1713
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1721..1727,1728..1745,1746..1751))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1766..1787
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1904..1921)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2075..2663)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2155..2174)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2834..3625,3626..3694))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3457..3476
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3695..3799)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3825..4280)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3962..3983)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4174..4193
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4407..4429
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4421..4438
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4422..4438
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4448..4467
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4448..4466
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      4487..4622
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4662..4783
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4673..4692)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4727..4746
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4855..5088
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(5175..5573)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(5592..5639)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(5687..6016)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5701..5836
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6001..6021
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     misc_feature    6072..6660
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             6178..6189
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6587..6607
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    6691..6715
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(6733..7443)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6813..6836)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(7011..7030)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     7280..7298
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(7840..7896)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(9061..9084)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      9084..9093
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(9100..9124)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(9208..9232)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(9229..9432)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(9258..9278)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        9433..9736
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    9759..9875
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 accaagcctc ctactatcat tatgaataat tttatatacc acagccaatt tgttatgtta
       61 aaccaattcc acaaacttgc ccatttatct aattccaata attcttgttc attcttttct
      121 tgctggtttt gcgattcttc aattaaggag tgtattaagc ttgtgtaatt gttaatttct
      181 ctgtcccact ccatccaggt cgtgtgattc caaatctgtt ccagagattt attactccaa
      241 ctagcattcc aaggcacagc agtggtgcaa atgagttttc cagagcaacc ccaaatcccc
      301 aggagctgtt gatcctttag gtatctttcc acagccagga ttcttgcctg gagctgcttg
      361 atgccccaga ctgtgagttg caacagatgc tgttgcgcct caatagccct cagcaaattg
      421 ttctgctgct gcactatacc agacaataat tgtctggcct gtaccgtcag cgtcattgac
      481 gctgcgccca tagtgcttcc tgctgctccc aagaacccaa ggaacaaagc tcctattccc
      541 actgctcttt tttctctctg caccactctt ctctttgcct tggtgggtgc tactcctaat
      601 ggttcaattt ttactacttt atatttatat aattcacttc tccaattgtc cctcatatct
      661 cctcctccag gtctgaagat cagcggccgc ttgctgtgcg gtggtcttac ttttgttttg
      721 ctcttcctct atcttgtcta aagcttcctt ggtgtctttt atctctatcc tttgatgcac
      781 acaatagagg gttgctactg tattatataa tgatctaagt tcttctgatc ctgtctgaag
      841 ggatggttgt agctgtccca gtatttgtct acagccttct gatgtttcta acaggccagg
      901 attaactgcg aatcgttcta gctccctgct tgcccatact atatgtttta atttatattt
      961 tttctttccc cctggcctta accgaatttt ttcccatcgc gatctaattc tcccccgctt
     1021 aatactgacg ctctcgcacc catctctctc cttctagcct ccgctagtca aaatttttgg
     1081 cgtactcacc agtcgccgcc cctcgcctct tgccgtgcgc gcttcagcaa gccgagtcct
     1141 gcgtcgagag agctcctctg gtttcccttt cgctttcaag tccctgttcg ggcgccactg
     1201 ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc agagtcacac
     1261 aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg cttaagcagt
     1321 gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca gagagacccg
     1381 tttatgtatc gagctaggca cttaaataca atatctctgc aatgcggcaa ttcagtggtt
     1441 cgtccaatcc atgtcagacc cgtctgttgc cttcctaata aggcacgatc gtaccacctt
     1501 acttccacca atcggcatgc acggtgcttt ttctctcctt gtaaggcatg ttgctaactc
     1561 atcgttacca tgttgcaaga ctacaagagt attgcataag actacattaa gcttgcagct
     1621 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc
     1681 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     1741 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     1801 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     1861 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     1921 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     1981 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     2041 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     2101 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     2161 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     2221 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     2281 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     2341 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     2401 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     2461 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     2521 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     2581 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     2641 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     2701 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     2761 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     2821 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     2881 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     2941 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     3001 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     3061 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     3121 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     3181 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     3241 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     3301 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     3361 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     3421 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     3481 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     3541 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     3601 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     3661 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     3721 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     3781 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa
     3841 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     3901 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     3961 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     4021 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     4081 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     4141 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     4201 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     4261 tgcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg
     4321 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     4381 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     4441 gcgcgcgtaa tacgactcac tatagggcga attgggtacg tccctaggcc tccaaaaaag
     4501 cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc tgcataaata
     4561 aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt taggggcggg
     4621 atgggcggag ttaggggcgg gatagctaga gccagacatg ataagataca ttgatgagtt
     4681 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     4741 tattgcttta tttgtaacca ttataagctg caataaacaa gttcctctca ctctctgata
     4801 ttcatttctt tgcaagttat aaatactgaa taataagatg acatgaacta ctactgctag
     4861 agattttcca cactgactaa aagggtctga gggatctcta gttaccagag tcacacaaca
     4921 gacgggcaca cactacttga agcactcaag gcaagcttta ttgaggctta agcagtgggt
     4981 tccctagtta gccagagagc tcccaggctc agatctggtc taaccagaga gacccagtac
     5041 aagcaaaaag cagatcttgt cttcgttggg agtgagctag cccttccagt cccccctttt
     5101 cttttaaaaa gtggctaaga tctacagctg ccttgtaagt cattggtctt aaaggtaccg
     5161 agctcgaatt gtgcttagcc ctcccacaca taaccagagg gcagcaattc acgaatccca
     5221 actgccgtcg gctgtccatc actgtccttc actatggctt tgatcccagg atgcagatcg
     5281 agaagcacct gtcggcaccg tccgcagggg ctcaagatgc ccctgttctc atttccgatc
     5341 gcgacgatac aagtcaggtt gccagctgcc gcagcagcag cagtgcccag caccacgagt
     5401 tctgcacaag gtcccccagt aaaatgatat acattgacac cagtgaagat gcggccgtcg
     5461 ctagagagag ctgcgctggc gacgctgtag tcttcagaga tggggatgct gttgattgta
     5521 gccgttgctc tttcaatgag ggtggattct tcttgagaca aaggcttggc catggtttag
     5581 ttcctcacct tgtcgtatta tactatgccg atatactatg ccgatgatta attgtcaaca
     5641 cgtgctgatc agatccgaaa atggatatac aagctcccgg gagctttttg caaaagccta
     5701 ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggcagag gcggcctcgg
     5761 cctctgcata aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg
     5821 gagttagggg cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgagat
     5881 gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac ctggttgctg
     5941 actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac
     6001 accctaactg acacacattc cacagaatta attccaaact cattactaac cggatatctg
     6061 cagaattcca ggcggggagg cggcccaaag ggagatccga ctcgtctgag ggcgaaggcg
     6121 aagacgcgga agaggccgca gagccggcag caggccgcgg gaaggaaggt ccgctggatt
     6181 gagggccgaa gggacgtagc agaaggacgt cccgcgcaga atccaggtgg caacacaggc
     6241 gagcagccat ggaaaggacg tcagcttccc cgacaacacc acggaattgt cagtgcccaa
     6301 cagccgagcc cctgtccagc agcgggcaag gcaggcggcg atgagttccg ccgtggcaat
     6361 agggaggggg aaagcgaaag tcccggaaag gagctgacag gtggtggcaa tgccccaacc
     6421 agtgggggtt gcgtcagcaa acacagtgca caccacgcca cgttgcctga caacgggcca
     6481 caactcctca taaagagaca gcaaccagga tttatacaag gaggagaaaa tgaaagccat
     6541 acgggaagca atagcatgat acaaaggcat taaagcagcg tatccacata gcgtaaaagg
     6601 agcaacatag ttaagaatac cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt
     6661 gtcgagcggc cgccactgtg ctggatatca accactttgt acaagaaagc tgggagatct
     6721 ctcgagctat cattacttgt acagctcgtc catgccgccg gtggagtggc ggccctcggc
     6781 gcgctcgtac tgttccacga tggtgtagtc ctcgttgtgg gaggtgatgt ccagcttgat
     6841 gttgacgttg taggcgccgg gcagctgcac gggcttcttg gccttgtagg tggtcttgac
     6901 ctcggcgtcg tagtggccgc cgtccttcag cttcagcctc tgcttgatct cgcccttcag
     6961 ggcgccgtcc tcggggtaca tccgctcgga ggaggcctcc cagcccatgg tcttcttctg
     7021 cattacgggg ccgtcggagg ggaagttggt gccgcgcagc ttcaccttgt agatgaactc
     7081 gccgtcctgc agggaggagt cctgggtcac ggtcaccacg ccgccgtcct cgaagttcat
     7141 cacgcgctcc cacttgaagc cctcggggaa ggacagcttc aagtagtcgg ggatgtcggc
     7201 ggggtgcttc acgtaggcct tggagccgta catgaactga ggggacagga tgtcccaggc
     7261 gaagggcagg gggccgccct tggtcacctt cagcttggcg gtctgggtgc cctcgtaggg
     7321 gcggccctcg ccctcgccct cgatctcgaa ctcgtggccg ttcacggagc cctccatgtg
     7381 caccttgaag cgcatgaact ccttgatgat ggccatgttg tcctcctcgc ccttgctcac
     7441 catcccccct gcggttttct gctggccctt cttaccaatc agagacttgt ggatgtgagg
     7501 aatgactcct cctccagcaa tggtggcctt gataagcgag tccagctcct cgtctccgcg
     7561 gatggccagc tgtaaatgtc tgggagtgat tcgcttcacc ttcagatcct tagaagcgtt
     7621 tccagccaac tccagcacct cagcggtgag atactccagg atagcagcac tgtaaaccgc
     7681 agctgtagct ccgacacggc catggctggt ggttcgtgtc ttcaggtgcc tgtggatacg
     7741 acctacaggg aactgcagac cagctctctg agatctggag acggctttcg tcttggcttt
     7801 tccactgtct ttacctgctt ttccacctgc catcctaggt ggcccgggat tctcttcgac
     7861 atcccctgct tgtttcaaca gggagaagtt agtggctccg gtacccatca cgcaaggctt
     7921 gatgtcttgg taggtgacct gctggtggta gtaggagccc ccgccggccg agtgcatgga
     7981 agaggacgcc atggcgttga aagagaagac aaactccttt cggtcacaca tgctgggcga
     8041 ctgcgagtga taccgcggga tgccttgcag ctcggctggg gcgttgtggc tgttctggtg
     8101 cagatacggc tgctccaggg agtgcgtgga gaggctgccg gacagggggt tggccgcggg
     8161 gttacagggg gacaggggct gctgcttgat ataagaggcg ccgctgttga gcgccgcgga
     8221 cgccgagggc ggccaggccg ccgccgagcc cgagtagacg gcgtgcggct ccatgacccc
     8281 accggcgccg gtgggcagca gcggggaggc gggcaccgag ctgtcgtggt gcgggtactc
     8341 gccggccgcc gcgccgccgc agccgcccat gtacgagtgg ccgccgttgg aaggcaggtg
     8401 gggcaccgag tggctgggca gggccatgcc gtccacgttg cccggcaagt ggccgttcat
     8461 catgcccagg ccgccctcca gcgccaggct gttgggcggg cacgagaggc cgccggccga
     8521 gccctggaag ccgtaggtgt ccgggaggtg gttgaagccg agcccgttca tcatgctgta
     8581 catgggcttg agcgcctggc atttccttcg gaagccgcgc ggccgccgcc gaaaggagcc
     8641 ctcctcgaac atgaactcgc tggccgggtc gatggtccag tagtggccct tgccgggccg
     8701 cccaaggccc ttgggtagct tgatgaagca ctcgttgagc gagaggttgt ggcgcacgga
     8761 gttcttccag ccctggtagg agccccggaa gaaggggaag cggctctgca ggaactggta
     8821 gatctcgctc agcgtcaggc gcttggtggg tgaactctgg atggccatga cgatgagcgc
     8881 gatgtaggaa tagggcggct tctccgggcg ccggatgccg gcgttggtct tcttggcctt
     8941 ggacgggccg gacgacgcgg ggtccatggc cgcgccgcct cccccgccgc cgccgccgcc
     9001 gccgccgtgc ggtggctgct gcttctcggg cgccgaagag aattcgaatc gatgggatcc
     9061 cttatcgtcg tcgtccttgt aatccatggt ggcggatcca gcctgctttt ttgtacaaac
     9121 ttgttgatat ctgcagaatt ccaccacact ggactagagt cggtgtcttc tatggaggtc
     9181 aaaacagcgt ggatggcgtc tccaggcgat ctgacggttc actaaacgag ctctgcttat
     9241 atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg agttgttacg
     9301 acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga cgtcaatggg
     9361 gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga tgtactgcca
     9421 aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa
     9481 gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt cattgacgtc
     9541 aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg
     9601 taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta tgggaacata
     9661 cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg ggccatttac
     9721 cgtaagttat gtaacgcgga actcccaagc ttatcgataa aattttgaat tttgtaattt
     9781 gtttttgtaa ttctttagtt tgtatgtctg ttgctattat gtctactatt ctttcccctg
     9841 cactgtaccc cccaatcccc ccttttcttt taaaagttaa ccgataccgt cgagatccgt
     9901 tcactaatcg aatggatctg tctctgtctc tctctccacc ttcttcttct attccttcgg
     9961 gcctgtcggg tcccctcggg gttgggaggt gggtctgaaa cgataatggt gaatatccct
    10021 gcctaactct attcactata gaaagtacag caaaaactat tcttaaacct
//
