LOCUS       sequence-466098-        9004 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Piggybac expression vector with integrin beta1 NPxY mutant (YYFF) 
            tagged with mRuby2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9004)
  AUTHORS   Conway JRW, Joshi O, Kaivola J, Follain G, Gounis M, Kuhl D, Ivaska 
            J
  TITLE     Dynamic regulation of integrin beta1 phosphorylation supports 
            invasion of breast cancer cells.
  JOURNAL   Nat Cell Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4.
  PUBMED    40419795
REFERENCE   2  (bases 1 to 9004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4."
COMMENT     Sequence Label: pPB.DEST-ITGB1(YYFF)-mRuby2
FEATURES             Location/Qualifiers
     source          1..9004
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   674..708
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1011..1390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1678..2694
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2618..2640
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2702..2721
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             5258..5968
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     protein_bind    complement(5989..6013)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(6046..6063)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6052..6276
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6323..6339)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6347..6363
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6371..6377,6378..6395,6396..6401))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6416..6437
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   6703..6765
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6816..6836)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6816..6834)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6855..6871)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6879..6895
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6903..6909,6910..6927,6928..6933))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6948..6969
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7086..7103)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7257..7845)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7337..7356)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8016..8807,8808..8876))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8639..8658
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8877..8981)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagcatg ggcccatctc gacattgatt
     1021 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1081 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     1141 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     1201 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1261 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     1321 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1381 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
     1441 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
     1501 gggggggggg ggggggcccc gggggggggg gggggggggg gggggggggg gggggggggc
     1561 gggggggggg ggcggggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct
     1621 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga
     1681 gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc
     1741 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
     1801 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc
     1861 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg
     1921 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
     1981 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
     2041 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
     2101 gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc
     2161 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc
     2221 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     2281 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc
     2341 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     2401 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     2461 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     2521 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     2581 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     2641 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     2701 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattcag atctgcggcc
     2761 gcctcgagaa gcttgatcaa caagtttgta caaaaaaagc aggctggcgc cggaaccaat
     2821 tcagtcgact ggatccggta ccaccgccat gaatttacaa ccaattttct ggattggact
     2881 gatcagttca gtttgctgtg tgtttgctca aacagatgaa aatagatgtt taaaagcaaa
     2941 tgccaaatca tgtggagaat gtatacaagc agggccaaat tgtgggtggt gcacaaattc
     3001 aacattttta caggaaggaa tgcctacttc tgcacgatgt gatgatttag aagccttaaa
     3061 aaagaagggt tgccctccag atgacataga aaatcccaga ggctccaaag atataaagaa
     3121 aaataaaaat gtaaccaacc gtagcaaagg aacagcagag aagctcaagc cagaggatat
     3181 tactcagatc caaccacagc agttggtttt gcgattaaga tcaggggagc cacagacatt
     3241 tacattaaaa ttcaagagag ctgaagacta tcccattgac ctctactacc ttatggacct
     3301 gtcttactca atgaaagacg atttggagaa tgtaaaaagt cttggaacag atctgatgaa
     3361 tgaaatgagg aggattactt cggacttcag aattggattt ggctcatttg tggaaaagac
     3421 tgtgatgcct tacattagca caacaccagc taagctcagg aacccttgca caagtgaaca
     3481 gaactgcacc agcccattta gctacaaaaa tgtgctcagt cttactaata aaggagaagt
     3541 atttaatgaa cttgttggaa aacagcgcat atctggaaat ttggattctc cagaaggtgg
     3601 tttcgatgcc atcatgcaag ttgcagtttg tggatcactg attggctgga ggaatgttac
     3661 acggctgctg gtgttttcca cagatgccgg gtttcacttt gctggagatg ggaaacttgg
     3721 tggcattgtt ttaccaaatg atggacaatg tcacctggaa aataatatgt acacaatgag
     3781 ccattattat gattatcctt ctattgctca ccttgtccag aaactgagtg aaaataatat
     3841 tcagacaatt tttgcagtta ctgaagaatt tcagcctgtt tacaaggagc tgaaaaactt
     3901 gatccctaag tcagcagtag gaacattatc tgcaaattct agcaatgtaa ttcagttgat
     3961 cattgatgca tacaattccc tttcctcaga agtcattttg gaaaacggca aattgtcaga
     4021 aggagtaaca ataagttaca aatcttactg caagaacggg gtgaatggaa caggggaaaa
     4081 tggaagaaaa tgttccaata tttccattgg agatgaggtt caatttgaaa ttagcataac
     4141 ttcaaataag tgtccaaaaa aggattctga cagctttaaa attaggcctc tgggctttac
     4201 ggaggaagta gaggttattc ttcagtacat ctgtgaatgt gaatgccaaa gcgaaggcat
     4261 ccctgaaagt cccaagtgtc atgaaggaaa tgggacattt gagtgtggcg cgtgcaggtg
     4321 caatgaaggg cgtgttggta gacattgtga atgcagcaca gatgaagtta acagtgaaga
     4381 catggatgct tactgcagga aagaaaacag ttcagaaatc tgcagtaaca atggagagtg
     4441 cgtctgcgga cagtgtgttt gtaggaagag ggataataca aatgaaattt attctggcaa
     4501 attctgcgag tgtgataatt tcaactgtga tagatccaat ggcttaattt gtggaggaaa
     4561 tggtgtttgc aagtgtcgtg tgtgtgagtg caaccccaac tacactggca gtgcatgtga
     4621 ctgttctttg gatactagta cttgtgaagc cagcaacgga cagatctgca atggccgggg
     4681 catctgcgag tgtggtgtct gtaagtgtac agatccgaag tttcaagggc aaacgtgtga
     4741 gatgtgtcag acctgccttg gtgtctgtgc tgagcataaa gaatgtgttc agtgcagagc
     4801 cttcaataaa ggagaaaaga aagacacatg cacacaggaa tgttcctatt ttaacattac
     4861 caaggtagaa agtcgggaca aattacccca gccggtccaa cctgatcctg tgtcccattg
     4921 taaggagaag gatgttgacg actgttggtt ctattttacg tattcagtga atgggaacaa
     4981 cgaggtcatg gttcatgttg tggagaatcc agagtgtccc actggtccag acatcattcc
     5041 aattgtagct ggtgtggttg ctggaattgt tcttattggc cttgcgttac tcctcatttg
     5101 gaagctttta atgataattc atgacagaag ggagtttgct aaatttgaaa aggagaaaat
     5161 gaatgccaaa tgggacacgg gtgaaaatcc tatttttaag agtgccgtaa caactgtggt
     5221 caatccgaag tttgagggaa aaggcggcgg cggccgcatg gtgtctaagg gcgaagagct
     5281 gatcaaggaa aatatgcgta tgaaggtggt catggaaggt tcggtcaacg gccaccaatt
     5341 caaatgcaca ggtgaaggag aaggcaatcc gtacatggga actcaaacca tgaggatcaa
     5401 agtcatcgag ggaggacccc tgccatttgc ctttgacatt cttgccacgt cgttcatgta
     5461 tggcagccgt acttttatca agtacccgaa aggcattcct gatttcttta aacagtcctt
     5521 tcctgagggt tttacttggg aaagagttac gagatacgaa gatggtggag tcgtcaccgt
     5581 catgcaggac accagccttg aggatggctg tctcgtttac cacgtccaag tcagaggggt
     5641 aaactttccc tccaatggtc ccgtgatgca gaagaagacc aagggttggg agcctaatac
     5701 agagatgatg tatccagcag atggtggtct gaggggatac actcatatgg cactgaaagt
     5761 tgatggtggt ggccatctgt cttgctcttt cgtaacaact tacaggtcaa aaaagaccgt
     5821 cgggaacatc aagatgcccg gtatccatgc cgttgatcac cgcctggaaa ggttagagga
     5881 aagtgacaat gaaatgttcg tagtacaacg cgaacacgca gttgccaagt tcgccgggct
     5941 tggtggtggg atggacgagc tgtacaagta gtaactcgag atatctagac ccagctttct
     6001 tgtacaaagt ggttgatcat cgatgatcta gagctcgctg atcagcctcg actgtgcctt
     6061 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     6121 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     6181 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     6241 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaacctgca
     6301 gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     6361 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     6421 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     6481 cgtgccagcg gatccattca tgaatgaatt catgtcgaca tactagttaa aagttttgtt
     6541 actttataga agaaattttg agtttttgtt tttttttaat aaataaataa acataaataa
     6601 attgtttgtt gaatttatta ttagtatgta agtgtaaata taataaaact taatatctat
     6661 tcaaattaat aaataaacct cgatatacag accgataaaa cacatgcgtc aattttacgc
     6721 atgattatct ttaacgtacg tcacaatatg attatctttc tagggttaat ctagtatacg
     6781 cgtatgcggc cgcttaatta atccagcttt tgttcccttt agtgagggtt aattgcgcgc
     6841 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     6901 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     6961 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     7021 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     7081 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     7141 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     7201 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     7261 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     7321 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     7381 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     7441 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     7501 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     7561 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     7621 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     7681 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     7741 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     7801 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     7861 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     7921 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     7981 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     8041 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     8101 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     8161 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     8221 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     8281 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     8341 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     8401 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     8461 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     8521 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     8581 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     8641 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     8701 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     8761 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     8821 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     8881 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     8941 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     9001 ccac
//
