LOCUS       sequence-466122-       11801 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-inducible lentiviral transfer vector with Src 
            constitutively-active mutant (Y527F).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11801)
  AUTHORS   Conway JRW, Joshi O, Kaivola J, Follain G, Gounis M, Kuhl D, Ivaska 
            J
  TITLE     Dynamic regulation of integrin beta1 phosphorylation supports 
            invasion of breast cancer cells.
  JOURNAL   Nat Cell Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4.
  PUBMED    40419795
REFERENCE   2  (bases 1 to 11801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4."
COMMENT     Sequence Label: pINDUCER20-Src(Y527F)
FEATURES             Location/Qualifiers
     source          1..11801
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2230..2248,2249..2271,2272..2290,2291..2313,2314..2332,
                     2333..2355,2356..2374,2375..2397,2398..2416,2417..2439,
                     2440..2458,2459..2481,2482..2500)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2533..2571
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2568..2592
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2701..2725
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2763..2772
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2769..2810
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     protein_bind    complement(4388..4412)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4465..4491
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        4577..5788
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5625..5644
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(5847..6464,6465..6470,6471..6506,6507..6509,6510..6545,
                     6546..6548,6549..6584,6585..6593)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6631..7204
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6798..6815)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7025..7044
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7220..8014
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7220..8014
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7274..7293)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7884..7903
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    8191..8779
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8244..8264)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8662..8673)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8854..9087
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9206..9224)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9292..9396
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9397..9465,9466..10257)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9615..9634)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10428..11016
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10917..10936
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11170..11187
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11427..11448
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11463..11468,11469..11486,11487..11493)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11501..11517
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11506..11528
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11525..11541
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11525..11541
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11591..11610
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
     2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2401 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2461 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg
     2521 tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
     2581 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
     2641 gaccgatcca gcctccgcgg ccccgaacta gtccagtgtg gtggaattct gcagatatca
     2701 acaagtttgt acaaaaaagc aggctggcgc cggaaccaat tcagtcgact ggatccggta
     2761 ccgccaccat ggggagcagc aagagcaagc ccaaggaccc cagccagcgc cggcgcagcc
     2821 tggagccacc cgacagcacc caccacgggg gattcccagc ctcgcagacc cccaacaaga
     2881 cagcagcccc cgacacgcac cgcaccccca gccgctcctt tgggaccgtg gccaccgagc
     2941 ccaagctctt cgggggcttc aacacttctg acaccgtcac gtcgccgcag cgtgccgggg
     3001 cactggctgg cggcgtcacc actttcgtgg ctctctatga ctacgagtcc cggactgaaa
     3061 cggacttgtc cttcaagaaa ggagaacgcc tgcagattgt caacaacacg gaaggtgact
     3121 ggtggctggc tcattccctc actacaggac agacgggcta catccccagt aactatgtcg
     3181 cgccctcaga ctccatccag gctgaagagt ggtactttgg gaagatcact cgtcgggagt
     3241 ccgagcggct gctgctcaac cccgaaaacc cccggggaac cttcttggtc cgggagagcg
     3301 agacgacaaa aggtgcctat tgcctctccg tttctgactt tgacaacgcc aaggggctca
     3361 atgtgaagca ctacaagatc cgcaagctgg acagcggcgg cttctacatc acctcacgca
     3421 cacagttcag cagcctgcag cagctggtgg cctactactc caaacatgct gatggcttgt
     3481 gccaccgcct gaccaacgtc tgccccacgt ccaagcccca gacccaggga ctcgccaagg
     3541 acgcgtggga aatcccccgg gagtcgctgc ggctggaggt gaagctgggg cagggctgct
     3601 ttggagaggt ctggatgggg acctggaacg gcaccaccag agtggccata aagactctga
     3661 agcccggcac catgtccccg gaggccttcc tgcaggaagc ccaagtgatg aagaagctcc
     3721 ggcatgagaa gctggttcag ctgtacgcag tggtgtcgga agagcccatc tacatcgtca
     3781 ctgagtacat gagcaagggg agcctcctgg atttcctgaa gggagagatg ggcaagtacc
     3841 tgcggctgcc acagctcgtc gatatggctg ctcagattgc atccggcatg gcctatgtgg
     3901 agaggatgaa ctacgtgcac cgagacctgc gggcggccaa catcctggtg ggggagaacc
     3961 tggtgtgcaa ggtggctgac tttgggctgg cacgcctcat cgaggacaac gagtacacag
     4021 cacggcaagg tgccaagttc cccatcaagt ggacagcccc cgaggcagcc ctctatggcc
     4081 ggttcaccat caagtcggat gtctggtcct tcggcatcct gctgactgag ctgaccacca
     4141 agggccgggt gccataccca gggatggtca acagggaggt gctggaccag gtggagaggg
     4201 gctaccgcat gccctgcccg cccgagtgcc ccgagtcgct gcatgacctc atgtgccagt
     4261 gctggcggaa ggaccctgag gagcggccca cttttgagta cctgcaggcc ttcctggagg
     4321 actacttcac ctcgacagag ccccagttcc agcctggaga gaacctatag tgactcgaga
     4381 tatctagacc cagctttctt gtacaaagtg gttgatatcc agcacagtgg cggccgctcg
     4441 agtctagagg gcccgcggtt cgaataccca tacgacgtcc cagactacgc ttagtaatga
     4501 ttaattaaac tagaaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagt
     4561 aacccaccca agatctggcc tccgcgccgg gttttggcgc ctcccgcggg cgcccccctc
     4621 ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga tccttccgcc
     4681 cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc ccagtatcag
     4741 cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt ctttccagag
     4801 agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg atctccgtgg
     4861 ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct agttccgtcg
     4921 cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca cttggtgagt
     4981 agcgggctgc tgggctggcc ggggctttcg tggccgccgg gccgctcggt gggacggaag
     5041 cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg ccctgaactg
     5101 ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga gtcttgaatg gaagacgctt
     5161 gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg gcaagaaccc
     5221 aaggtcttga ggccttcgct aatgcgggaa agctcttatt cgggtgagat gggctggggc
     5281 accatctggg gaccctgacg tgaagtttgt cactgactgg agaactcggt ttgtcgtctg
     5341 ttgcgggggc ggcagttatg gcggtgccgt tgggcagtgc acccgtacct ttgggagcgc
     5401 gcgccctcgt cgtgtcgtga cgtcacccgt tctgttggct tataatgcag ggtggggcca
     5461 cctgccggta ggtgtgcggt aggcttttct ccgtcgcagg acgcagggtt cgggcctagg
     5521 gtaggctctc ctgaatcgac aggcgccgga cctctggtga ggggagggat aagtgaggcg
     5581 tcagtttctt tggtcggttt tatgtaccta tcttcttaag tagctgaagc tccggttttg
     5641 aactatgcgc tcggggttgg cgagtgtgtt ttgtgaagtt ttttaggcac cttttgaaat
     5701 gtaatcattt gggtcaatat gtaattttca gtgttagact agtaaattgt ccgctaaatt
     5761 ctggccgttt ttggcttttt tgttagacga agcttggtac cgagctcgga tctccacccc
     5821 gtaccggtcc tgcagtcgaa ttcaccatgt ctagactgga caagagcaaa gtcataaacg
     5881 gagctctgga attactcaat ggtgtcggta tcgaaggcct gacgacaagg aaactcgctc
     5941 aaaagctggg agttgagcag cctaccctgt actggcacgt gaagaacaag cgggccctgc
     6001 tcgatgccct gccaatcgag atgctggaca ggcatcatac ccacttctgc cccctggaag
     6061 gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc ataccgctgt gctctcctct
     6121 cacatcgcga cggggctaaa gtgcatctcg gcacccgccc aacagagaaa cagtacgaaa
     6181 ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt ctccctggag aacgcactgt
     6241 acgctctgtc cgccgtgggc cactttacac tgggctgcgt attggaggaa caggagcatc
     6301 aagtagcaaa agaggaaaga gagacaccta ccaccgattc tatgccccca cttctgagac
     6361 aagcaattga gctgttcgac cggcagggag ccgaacctgc cttccttttc ggcctggaac
     6421 taatcatatg tggcctggag aaacagctaa agtgcgaaag cggcgggccg accgacgccc
     6481 ttgacgattt tgacttagac atgctcccag ccgatgccct tgacgacttt gaccttgata
     6541 tgctgcctgc tgacgctctt gacgattttg accttgacat gctccccggg taactaagta
     6601 aggatccgcg gccgcactag aggaattccg cccctctccc cccccccccc ctaacgttac
     6661 tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat
     6721 attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat
     6781 tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga
     6841 agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca
     6901 gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac
     6961 acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt
     7021 caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca
     7081 ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt
     7141 aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg
     7201 ataagcttac cggtccacca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     7261 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     7321 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     7381 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg
     7441 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     7501 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     7561 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     7621 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     7681 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     7741 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     7801 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     7861 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     7921 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     7981 cgccttctat cgccttcttg acgagttctt ctgatgtaca agtaaagcgg ccgcgactct
     8041 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     8101 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgtttag tccctcccaa
     8161 ttcgatatca agcttatcgc gatagatcct aatcaacctc tggattacaa aatttgtgaa
     8221 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     8281 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     8341 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     8401 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     8461 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     8521 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     8581 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     8641 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     8701 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     8761 ctttgggccg cctccccgcc tgagatcctt taagaccaat gacttacaag gcagctgtag
     8821 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     8881 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     8941 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     9001 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     9061 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     9121 tataacttgc aaagaaatga atatcagaga gtgagaggcc cgggttaatt aaggaaaggg
     9181 ctagatcatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     9241 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     9301 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     9361 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     9421 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     9481 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     9541 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     9601 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
     9661 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     9721 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     9781 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     9841 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     9901 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     9961 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
    10021 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
    10081 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
    10141 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
    10201 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
    10261 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
    10321 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
    10381 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
    10441 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
    10501 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
    10561 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
    10621 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
    10681 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
    10741 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
    10801 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
    10861 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
    10921 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    10981 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    11041 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
    11101 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
    11161 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
    11221 ctccccgcgc gttggccgat tcattaatgc agcaagctca tggctgacta atttttttta
    11281 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
    11341 tttttggagg cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt tcccgactgg
    11401 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
    11461 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
    11521 cacacaggaa acagctatga catgattacg aatttcacaa ataaagcatt tttttcactg
    11581 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg
    11641 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc
    11701 aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa
    11761 agaaattgta tttgttaaat atgtactaca aacttagtag t
//
