LOCUS       sequence-466129-        1687 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transient overexpression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1687)
  TITLE     GMCL1 Controls 53BP1 Stability and Modulates Paclitaxel Sensitivity 
            in Cancer
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3_GMCL2-FLAG
FEATURES             Location/Qualifiers
     source          1..1687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1617..1640
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1653..1676
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ccgccagtgt gctggaattc gccaccatgg gatcgtcgag cagccgggtg ctgggccagc
       61 cgaggcgagc ccttgcccag caggaacagg gtgccagggc caggggctcg gcccggaggc
      121 cggacactgg agacgatgcg gcgagctacg gcttctgtta ctgcccgggc agtcacaagc
      181 gcaagcggag cagcggggcc tgccgctact gtgacccgga ctcgcacagg gaggagcatg
      241 aggaggaggg ggacaagcag cagccgctcc tcaacacccc tgcaaggaaa aaattaagga
      301 gtacatccaa atatatttat caaacattat ttttgaatgg tgaaaacagt gacattaaga
      361 tttgtgctct aggagaagaa tggcgattac acaaaatata tttatgtcaa tctggctact
      421 tttctagtat gttcagtggt tcttggaaag aatccagcat gaatattatt gaactggaga
      481 ttcctgacca gaacattgat gtagacgcac tgcaggttgc gtttggttca ctgtatcgag
      541 atgatgtctt gataaaaccc agtcgagttg ttgccatttt ggcagcagct tgtatgctgc
      601 agctggatgg tttaatacag cagtgtggtg agacaatgaa ggaaacaatt aatgtgaaaa
      661 ctgtatgcgg ttattacaca tcagtagaga tctatggatt agattctgta aagaaaaagt
      721 gccttgaatg gcttctaaac aatttgatga ctcaccagaa tgttaaactt tttaaagaac
      781 tcggtataaa tgtcatgaaa cagctcattg gttcctctaa cttatttgtg atgcaagtgg
      841 agatggatgt atacaccact ctaaaaaagt ggatgttcct tcaacttgtg ccttcttgga
      901 atggatcttt aaaacagctt ttgacagaaa cagatgtctg gttttctaaa cagagaaaag
      961 attttgaagg tatggccttt cttgaaactg aaccaggaaa accatttgtg tcagtattca
     1021 gacatttaag gttacaatat attatcagtg acctagcttc tgcaagaatt attgaacaag
     1081 atggtatagt accttcagaa tggctgtctt ctgtgtataa acagcagtgg tttgctatgc
     1141 tgcgggcaga acaagaccgt gaggtagggc ctcaagaaat caataaagaa gacctagagg
     1201 gaaatagcat gaggtgtggt agaaagcttg ccaaagatgg tgaatactac tggtgttgga
     1261 cgggttttaa cttcggcttt gacctacttg taatttacac caatggatac atcattttca
     1321 aacgcaatac actgaatcag ccacgcagcg ggtctgtcag tttacggcct cgaaggagca
     1381 tagcatttag attacgcttg gcttcttttg atagtagtgg aaaactagta tgtagtagaa
     1441 caactggcta tcaaatactt atacttaaaa aggatcagga acaagtggtg atgaacttgg
     1501 acagcaggtt tctgaccttc cctttatata tctgctgtaa cttcttgtat atatcaccag
     1561 aaaaaggaat tgaaaataat cgtcacccag aaaatccaga aaacggagga tcaggagact
     1621 acaaggacga cgatgacaag ggaggatcag gagattataa agatgatgat gataaatgag
     1681 ggcccta
//
