LOCUS       sequence-46615-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Zhang Y, Zhang F, Li X, Baller JA, Qi Y, Starker CG, Bogdanove AJ, 
            Voytas DF
  TITLE     TALENs enable efficient plant genome engineering.
  JOURNAL   Plant Physiol. 2012 Nov 2.
  PUBMED    23124327
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2012 Nov 2."
COMMENT     Sequence Label: pZHY013
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    75..99
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             121..186
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             193..213
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 caataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat
       61 gcttttttat aatgccaact ttgtacaaaa aagcaggctc cgaattcgcc cttcaccatg
      121 gattataagg atcacgatgg cgactacaag gaccacgata ttgactacaa agacgatgac
      181 gataaaatgg ctcctaagaa aaagcgcaaa gtcggtatcc atggcgttcc ctctagataa
      241 cgcaggatcc cagctggtga agtccgagct ggaagaaaaa aagagcgagc tgcgccacaa
      301 gctcaagtac gtgccccacg agtacatcga gctgatcgag atcgcccgca acagcaccca
      361 agaccgcatc ctggagatga aagtgatgga gttcttcatg aaggtgtacg gctaccgcgg
      421 caagcacctg ggcggctccc gcaagcccga tggcgccatc tacaccgtgg gctcccccat
      481 cgactatggc gtcattgtcg acaccaaggc ctactccggc ggctacaact tacccatcgg
      541 tcaggccgac gagatgcaac gctacgtgaa ggagaaccag acccgcaata agcacattaa
      601 tcccaacgag tggtggaagg tgtacccctc ctccgtgacc gagttcaaat tcctgttcgt
      661 gtccggccac ttcaagggca attataaggc ccaactgacc cgcctgaacc acaagaccaa
      721 ctgcaacggc gccgtgctgt ccgtggagga actgctgatc ggcggcgaga tgatcaaggc
      781 tggtaccctg accctggaag aggtgcgccg caagttcaac aatggtgaaa tcaatttcag
      841 gtccggcggc g
//
