LOCUS       sequence-466151-        9176 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral overexpression vector NADK (D184A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9176)
  AUTHORS   Flickinger KM, Mellado Fritz CA, Huggler KS, Wade GM, Chang GR, Fox 
            KC, Simcox JA, Cantor JR
  TITLE     Cytosolic NADK is conditionally essential for folate-dependent 
            nucleotide synthesis.
  JOURNAL   Nat Metab. 2025 May 2. doi: 10.1038/s42255-025-01272-3.
  PUBMED    40316835
REFERENCE   2  (bases 1 to 9176)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Metab. 
            2025 May 2. doi: 10.1038/s42255-025-01272-3."
COMMENT     Sequence Label: pLJC6-NADK (D184A)-3xFLAG
FEATURES             Location/Qualifiers
     source          1..9176
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    580..705
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             752..932
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(933..1159)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1187..1207)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1187..1205)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1226..1242)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1226..1242)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1239..1261)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1250..1266
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1274..1280,1281..1298,1299..1304))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1319..1340
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1457..1474)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1628..2216)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1708..1727)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2387..3178,3179..3247))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3010..3029
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3248..3352)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3378..3833)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3515..3536)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3727..3746
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3960..3982
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3974..3991
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3975..3991
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4001..4020
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4001..4019
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      4040..4175
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4215..4336
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4226..4245)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4280..4299
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4408..4641
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(4713..5108)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(5117..5627)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5180..5198)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    5676..5793
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(5841..5906)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        complement(7301..8512)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(7445..7464)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             complement(8801..8845)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    join(9030..9176,1..87)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 aattgtctgg cctgtaccgt cagcgtcatt gacgctgcgc ccatagtgct tcctgctgct
       61 cccaagaacc caaggaacaa agctcctatt cccactgctc ttttttctct ctgcaccact
      121 cttctctttg ccttggtggg tgctactcct aatggttcaa tttttactac tttatattta
      181 tataattcac ttctccaatt gtccctcata tctcctcctc caggtctgaa gatcagctgc
      241 tgcttgctgt gcggtggtct tacttttgtt ttgctcttcc tctatcttgt ctaaagcttc
      301 cttggtgtct tttatctcta tcctttgatg cacacaatag agggttgcta ctgtattata
      361 taatgatcta agttcttctg atcctgtctg aagggatggt tgtagctgtc ccagtatttg
      421 tctacagcct tctgatgttt ctaacaggcc aggattaact gcgaatcgtt ctagctccct
      481 gcttgcccat actatatgtt ttaatttata ttttttcttt ccccctggcc ttaaccgaat
      541 tttttcccat cgcgatctaa ttctcccccg cttaatactg acgctctcgc acccatctct
      601 ctccttctag cctccgctag tcaaaatttt tggcgtactc accagtcgcc gcccctcgcc
      661 tcttgccgtg cgcgcttcag caagccgagt cctgcgtcga gagagctcct ctggtttccc
      721 tttcgctttc aagtccctgt tcgggcgcca ctgctagaga ttttccacac tgactaaaag
      781 ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc
      841 actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc
      901 caggctcaga tctggtctaa ccagagagac ccgtttatgt atcgagctag gcacttaaat
      961 acaatatctc tgcaatgcgg caattcagtg gttcgtccaa tccatgtcag acccgtctgt
     1021 tgccttccta ataaggcacg atcgtaccac cttacttcca ccaatcggca tgcacggtgc
     1081 tttttctctc cttgtaaggc atgttgctaa ctcatcgtta ccatgttgca agactacaag
     1141 agtattgcat aagactacat taagcttgca gctccagctt ttgttccctt tagtgagggt
     1201 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     1261 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     1321 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     1381 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     1441 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     1501 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     1561 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     1621 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     1681 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     1741 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     1801 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     1861 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1921 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     1981 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     2041 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     2101 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     2161 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     2221 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     2281 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     2341 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     2401 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     2461 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     2521 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     2581 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     2641 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     2701 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     2761 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     2821 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     2881 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     2941 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     3001 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     3061 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     3121 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     3181 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     3241 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     3301 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     3361 cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     3421 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     3481 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     3541 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     3601 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     3661 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     3721 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     3781 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc
     3841 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     3901 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     3961 ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg
     4021 cgaattgggt acgtccctag gcctccaaaa aagcctcctc actacttctg gaatagctca
     4081 gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga
     4141 gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg cgggatagct
     4201 agagccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     4261 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     4321 ctgcaataaa caagttcctc tcactctctg atattcattt ctttgcaagt tataaatact
     4381 gaataataag atgacatgaa ctactactgc tagagatttt ccacactgac taaaagggtc
     4441 tgagggatct ctagttacca gagtcacaca acagacgggc acacactact tgaagcactc
     4501 aaggcaagct ttattgaggc ttaagcagtg ggttccctag ttagccagag agctcccagg
     4561 ctcagatctg gtctaaccag agagacccag tacaagcaaa aagcagatct tgtcttcgtt
     4621 gggagtgaat tagcccttcc agtcccccct tttcttttaa aaagtggcta agatctacag
     4681 ctgccttgta agtcattggt cttaaaggta ccttagccct cccacacata accagagggc
     4741 agcaattcac gaatcccaac tgccgtcggc tgtccatcac tgtccttcac tatggctttg
     4801 atcccaggat gcagatcgag aagcacctgt cggcaccgtc cgcaggggct caagatgccc
     4861 ctgttctcat ttccgatcgc gacgatacaa gtcaggttgc cagctgccgc agcagcagca
     4921 gtgcccagca ccacgagttc tgcacaaggt cccccagtaa aatgatatac attgacacca
     4981 gtgaagatgc ggccgtcgct agagagagct gcgctggcga cgctgtagtc ttcagagatg
     5041 gggatgctgt tgattgtagc cgttgctctt tcaatgaggg tggattcttc ttgagacaaa
     5101 ggaaccatgg atccccctgg ggagagaggt cggtgattcg gtcaacgagg gagccgactg
     5161 ccgacgtgcg ctccggaggc ttgcagaatg cggaacaccg cgcgggcagg aacagggccc
     5221 acactaccgc cccacacccc gcctcccgca ccgccccttc ccggccgctg ctctcggcgc
     5281 gccctgctga gcagccgcta ttggccacag cccatcgcgg tcggcgcgct gccattgctc
     5341 cctggcgctg tccgtctgcg agggtactag tgagacgtgc ggcttccgtt tgtcacgtcc
     5401 ggcacgccgc gaaccgcaag gaaccttccc gacttagggg cggagcagga agcgtcgccg
     5461 gggggcccac aagggtagcg gcgaagatcc gggtgacgct gcgaacggac gtgaagaatg
     5521 tgcgagaccc agggtcggcg ccgctgcgtt tcccggaacc acgcccagag cagccgcgtc
     5581 cctgcgcaaa cccagggctg ccttggaaaa ggcgcaaccc caaccccctc gagccgcggc
     5641 caaagtggat ctctgctgtc cctgtaataa acccgaaaat tttgaatttt tgtaatttgt
     5701 ttttgtaatt ctttagtttg tatgtctgtt gctattatgt ctactattct ttcccctgca
     5761 ctgtaccccc caatcccccc ttttctttta aaattgtgga tgaatactgc catttgtctc
     5821 gaggtcgaga attcttatta cttgtcatcg tcatccttgt aatcaatgtc atgatcttta
     5881 taatcaccgt catggtcttt gtagtcgcct gcggccgcgc tgcctccgcc ctcctcttct
     5941 tcttcctctt cctcctcaaa atgagcctgc ttcttgcgaa cgttccaatg aagacattga
     6001 gccagggatt caaaccaatc tgataccggg tcgcgcacac aaatgctcgg cagaggatag
     6061 caggaagtgg tgatacttat ggaatccccg tgccggattt cctgccgttt tctcccatcg
     6121 aaggacaccc aagcggtatt ccgcgcttca ggggacagca taattttcaa ctctactccg
     6181 gccggtacta ctatcggtcg gaaagagagt gagtgaggac aaatcggtgt gatcattatg
     6241 gccggcacgt ttgggtggat cattgacgca cctgcagcgg ctgcataggc tgtggacccg
     6301 gtgggtgtgc tcacgataac cccatcacct tggaccgtgg taatcaaatg accgtccaga
     6361 tatacgtcta cgttactgag gtaggaactc gggccgcggt cgataacaac ttcgttcagg
     6421 acttgatatt gcatagcctg tttgcctaca tccatgtcca ggccggctgc ttgactcccg
     6481 ttttctccga gaccgttatg gacggctgtt ttcttccctc gcagttcctt tacaaccctg
     6541 actttcaggc gggagcgcaa aacaacggcg gcgttaccct ctatgacctg cgttacctga
     6601 gattgaaagt tctcaaaaga gaatggcgtg agaaaaccaa gagagccgag atggaatgcc
     6661 atgacaggtg gaacgctgcc ctgaaaaagg gaggatgcat acagaagcgt cccggcccct
     6721 cccaaacaga ttataaagtc gatctgattg gatatgtcgt cgtaatcttc ccgaaacgta
     6781 caaaattttt ttttcacagc gccaaaactt tcatcagaag cgatcgccgg gtcctcgagt
     6841 actttctttt cgacgtaaac gatcatattt tcttccatca agtgtgtgca aagctctttg
     6901 aacggttgga ggagtgacgc atcccgcatt tttttgatta cgagaacgga ctttggactc
     6961 ttattccacg tgagcctctg ggaagctgga tcctgaatgt gcatgattgt ctgcggattc
     7021 tgaagaacac atgccttggg cccaaatgtg gtcacaggac aaggcccgtg gagacttcta
     7081 gttcggcgga attccttagt agacccgagg gctggggacg cagacaagct tctgctcttt
     7141 gctctgcctc gtataggatg gttatagctc catgtttcgt caccatggca cgctgaacag
     7201 caatatgcgg cagcgtctgg gctaagctct ttattcatcg tcattttctc ctgctccatc
     7261 tccattgtca ggttaattaa accggtagcg ctagcgcttc gtctaacaaa aaagccaaaa
     7321 acggccagaa tttagcggac aatttactag tctaacactg aaaattacat attgacccaa
     7381 atgattacat ttcaaaaggt gcctaaaaaa cttcacaaaa cacactcgcc aaccccgagc
     7441 gcatagttca aaaccggagc ttcagctact taagaagata ggtacataaa accgaccaaa
     7501 gaaactgacg cctcacttat ccctcccctc accagaggtc cggcgcctgt cgattcagga
     7561 gagcctaccc taggcccgaa ccctgcgtcc tgcgacggag aaaagcctac cgcacaccta
     7621 ccggcaggtg gccccaccct gcattataag ccaacagaac gggtgacgtc acgacacgac
     7681 gagggcgcgc gctcccaaag gtacgggtgc actgcccaac ggcaccgcca taactgccgc
     7741 ccccgcaaca gacgacaaac cgagttctcc agtcagtgac aaacttcacg tcagggtccc
     7801 cagatggtgc cccagcccat ctcacccgaa taagagcttt cccgcattag cgaaggcctc
     7861 aagaccttgg gttcttgccg cccaccatgc cccccacctt gtttcaacga cctcacagcc
     7921 cgcctcacaa gcgtcttcca ttcaagactc gggaacagcc gccattttgc tgcgctcccc
     7981 ccaaccccca gttcagggca accttgctcg cggacccaga ctacagccct tggcggtctc
     8041 tccacacgct tccgtcccac cgagcggccc ggcggccacg aaagccccgg ccagcccagc
     8101 agcccgctac tcaccaagtg acgatcacag cgatccacaa acaagaaccg cgacccaaat
     8161 cccggctgcg acggaactag ctgtgccaca cccggcgcgt ccttatataa tcatcggcgt
     8221 tcaccgcccc acggagatcc ctccgcagaa tcgccgagaa gggactactt ttcctcgcct
     8281 gttccgctct ctggaaagaa aaccagtgcc ctagagtcac ccaagtcccg tcctaaaatg
     8341 tccttctgct gatactgggg ttctaaggcc gagtcttatg agcagcgggc cgctgtcctg
     8401 agcgtccggg cggaaggatc aggacgctcg ctgcgccctt cgtctgacgt ggcagcgctc
     8461 gccgtgagga ggggggcgcc cgcgggaggc gccaaaaccc ggcgcggagg ccagatcccg
     8521 ctcgaggcta gtctcgtgat cgataccgtc gagatccgtt cactaatcga atggatctgt
     8581 ctctgtctct ctctccacct tcttcttcta ttccttcggg cctgtcgggt cccctcgggg
     8641 ttgggaggtg ggtctgaaac gataatggtg aatatccctg cctaactcta ttcactatag
     8701 aaagtacagc aaaaactatt cttaaaccta ccaagcctcc tactatcatt atgaataatt
     8761 ttatatacca cagccaattt gttatgttaa accaattcca caaacttgcc catttatcta
     8821 attccaataa ttcttgttca ttcttttctt gctggttttg cgattcttca attaaggagt
     8881 gtattaagct tgtgtaattg ttaatttctc tgtcccactc catccaggtc gtgtgattcc
     8941 aaatctgttc cagagattta ttactccaac tagcattcca aggcacagca gtggtgcaaa
     9001 tgagttttcc agagcaaccc caaatcccca ggagctgttg atcctttagg tatctttcca
     9061 cagccaggat tcttgcctgg agctgcttga tgccccagac tgtgagttgc aacagatgct
     9121 gttgcgcctc aatagccctc agcaaattgt tctgctgctg cactatacca gacaat
//
