LOCUS       sequence-466210-        5747 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE2010m is an enhancer sequence, designed to drive AAV-mediated 
            transgene expression in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5747)
  AUTHORS   Ben-Simon Y, Hooper M, Narayan S, Daigle TL, Dwivedi D, Way SW, 
            Oster A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya
            X, Roth JR, Alexander JR, Allen S, Amster A, Arbuckle J, Ayala A, 
            Baker PM, Bakken TE, Barcelli T, Barta S, Bendrick J, Bertagnolli D,
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chance RK, Chavan S, Clark M, 
            Colbert K, Collman F, Daniel S, Departee M, DiValentin P, Donadio N,
            Dotson N, Egdorf T, Fliss T, Gabitto M, Garcia J, Gary A, Gasperini 
            M, Gloe J, Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, 
            Halterman C, Haradon Z, Hastings SD, Helback O, Ho W, Hockemeyer D, 
            Huang C, Huff S, Hunker A, Johansen N, Jones D, Juneau Z, Kalmbach 
            B, Kannan M, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, Lee AY, 
            Leibly M, Lenz GH, Li S, Liang E, Lusk N, Madigan Z, Malloy J, 
            Malone J, McCue R, Melchor J, Mollenkopf T, Moosman S, Morin E, 
            Newman D, Ng L, Ngo K, Omstead V, Otto S, Oyama A, Pena N, Pham T, 
            Phillips E, Pom CA, Potekhina L, Ransford S, Ray PL, Rette D, 
            Reynoldson C, Rimorin C, Rocha D, Ruiz A, Sanchez REA, Sawyer L, 
            Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shepard N, Shulga L, 
            Sigler AR, Siverts L, Soliman S, Somasundaram S, Staats B, Stewart 
            K, Szelenyi E, Tieu M, Trader C, Tran A, van Velthoven CTJ, Walker 
            M, Wang Y, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T, 
            Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith
            KA, Esposito L, Waters J, Thyagarajan B, Yao S, Lein ES, Zeng H, 
            Levi BP, Ngai J, Ting JT, Tasic B
  TITLE     A suite of enhancer AAVs and transgenic mouse lines for genetic 
            access to cortical cell types.
  JOURNAL   Cell. 2025 May 16:S0092-8674(25)00513-6. doi: 
            10.1016/j.cell.2025.05.002.
  PUBMED    40403729
REFERENCE   2  (bases 1 to 5747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 16:S0092-8674(25)00513-6. doi: 10.1016/j.cell.2025.05.002."
COMMENT     Sequence Label: AiP1199 - pAAV-AiE2010m-minBG-IRES2-iCre-HGHpA
FEATURES             Location/Qualifiers
     source          1..5747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(16..35)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     135..157
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(195..213)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        281..385
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(386..454,455..1246)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(604..623)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1417..2005
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1906..1925
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   2067..2196
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     misc_feature    2876..3449
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2899..3451
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3043..3060)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3270..3289
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3475..3495
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4560..5036
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4759..4778)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   5076..5216
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5076..5205
                     /label=AAV2 ITR
     rep_origin      5291..5746
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5378..5397)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5588..5609
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
       61 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
      121 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
      181 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
      241 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
      301 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
      361 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
      421 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
      481 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
      541 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
      601 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
      661 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
      721 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
      781 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
      841 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
      901 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
      961 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     1021 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     1081 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     1141 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     1201 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     1261 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     1321 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     1381 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1441 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     1501 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     1561 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     1621 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     1681 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     1741 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     1801 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     1861 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     1921 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     1981 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     2041 cttttgctgg ccttttgctc acatgtcctg caggcagctg cgcgctcgct cgctcactga
     2101 ggccgcccgg gcgtcgggcg acctttggtc gcccggcctc agtgagcgag cgagcgcgca
     2161 gagagggagt ggccaactcc atcactaggg gttcctgcgg ccgcacgcgt atgtgtcttt
     2221 tactctgatc ctcctgtttt taccttccaa gtgctggaat cacagacata taccactgtg
     2281 catagcatca ttacaatgtt atagtttttc acactatgcc ttgacttttt ggaaaggcaa
     2341 accacctctt ggatttctcc ttccttctct atctctctct ctctctcttc ctccctccgt
     2401 ccctccatct cttcctcctt cccattttct tctctcccta tttggacaca atataaaata
     2461 atttagatga ggtgagttaa attgtgaaca aagtatgtgc ctatacatgg ttgtaaatca
     2521 gcttatcaaa gtgtaatatt agaagaattt ataaaaatga taaaattcat actcaaagtt
     2581 ctgtgtaaag caataatagc tttatctcct tttagttatc ttgagtcttt ctatgactaa
     2641 caactccctc ataggcatct taaagagcag taagcataag tagattccaa atgggaaggg
     2701 agaagtgtga accatcactt tcatccagac ttgtagatat atctgctgca ttttcagaaa
     2761 ccagaaacag acagtgttct ttatctccat tgagtctagt gtagcaacag agctcgggct
     2821 gggcataaaa gtcagggcag agccatctat tgcttacatt tgcttctggg atccgcccct
     2881 ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     2941 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     3001 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     3061 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     3121 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     3181 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     3241 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     3301 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     3361 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
     3421 tggttttcct ttgaaaaaca cgatgataat atggccacag ctagcaccat ggtgcccaag
     3481 aagaagagga aagtctccaa cctgctgact gtgcaccaaa acctgcctgc cctccctgtg
     3541 gatgccacct ctgatgaagt caggaagaac ctgatggaca tgttcaggga caggcaggcc
     3601 ttctctgaac acacctggaa gatgctcctg tctgtgtgca gatcctgggc tgcctggtgc
     3661 aagctgaaca acaggaaatg gttccctgct gaacctgagg atgtgaggga ctacctcctg
     3721 tacctgcaag ccagaggcct ggctgtgaag accatccaac agcacctggg ccagctcaac
     3781 atgctgcaca ggagatctgg cctgcctcgc ccttctgact ccaatgctgt gtccctggtg
     3841 atgaggagaa tcagaaagga gaatgtggat gctggggaga gagccaagca ggccctggcc
     3901 tttgaacgca ctgactttga ccaagtcaga tccctgatgg agaactctga cagatgccag
     3961 gacatcagga acctggcctt cctgggcatt gcctacaaca ccctgctgcg cattgccgaa
     4021 attgccagaa tcagagtgaa ggacatctcc cgcaccgatg gtgggagaat gctgatccac
     4081 attggcagga ccaagaccct ggtgtccaca gctggtgtgg agaaggccct gtccctgggg
     4141 gttaccaagc tggtggagag atggatctct gtgtctggtg tggctgatga ccccaacaac
     4201 tacctgttct gccgggtcag aaagaatggt gtggctgccc cttctgccac ctcccaactg
     4261 tccacccggg ccctggaagg gatctttgag gccacccacc gcctgatcta tggtgccaag
     4321 gatgactctg ggcagagata cctggcctgg tctggccact ctgccagagt gggtgctgcc
     4381 agggacatgg ccagggctgg tgtgtccatc cctgaaatca tgcaggctgg tggctggacc
     4441 aatgtgaaca ttgtgatgaa ctacatcaga aacctggact ctgagactgg ggccatggtg
     4501 aggctgctcg aggatgggga ctaatgaggc gcgccgcggc cgctgctcga gagatctacg
     4561 ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag
     4621 tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct
     4681 tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggaagacaa
     4741 cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc
     4801 tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct cccgagttgt
     4861 tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg
     4921 gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt
     4981 ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct gtccttctga
     5041 ttttgtaggt aaccacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc
     5101 cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg
     5161 cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg
     5221 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag
     5281 caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     5341 agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc
     5401 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     5461 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca
     5521 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     5581 tttaatagtg gactcttgtt ccaaactgga acaacactca actctatctc gggctattct
     5641 tttgatttat aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa
     5701 caaaaattta acgcgaattt taacaaaata ttaacgttta caatttt
//
