LOCUS       sequence-466239-        5127 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE2013m is an enhancer sequence, designed to drive AAV-mediated 
            transgene expression in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5127)
  AUTHORS   Ben-Simon Y, Hooper M, Narayan S, Daigle TL, Dwivedi D, Way SW, 
            Oster A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya
            X, Roth JR, Alexander JR, Allen S, Amster A, Arbuckle J, Ayala A, 
            Baker PM, Bakken TE, Barcelli T, Barta S, Bendrick J, Bertagnolli D,
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chance RK, Chavan S, Clark M, 
            Colbert K, Collman F, Daniel S, Departee M, DiValentin P, Donadio N,
            Dotson N, Egdorf T, Fliss T, Gabitto M, Garcia J, Gary A, Gasperini 
            M, Gloe J, Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, 
            Halterman C, Haradon Z, Hastings SD, Helback O, Ho W, Hockemeyer D, 
            Huang C, Huff S, Hunker A, Johansen N, Jones D, Juneau Z, Kalmbach 
            B, Kannan M, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, Lee AY, 
            Leibly M, Lenz GH, Li S, Liang E, Lusk N, Madigan Z, Malloy J, 
            Malone J, McCue R, Melchor J, Mollenkopf T, Moosman S, Morin E, 
            Newman D, Ng L, Ngo K, Omstead V, Otto S, Oyama A, Pena N, Pham T, 
            Phillips E, Pom CA, Potekhina L, Ransford S, Ray PL, Rette D, 
            Reynoldson C, Rimorin C, Rocha D, Ruiz A, Sanchez REA, Sawyer L, 
            Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shepard N, Shulga L, 
            Sigler AR, Siverts L, Soliman S, Somasundaram S, Staats B, Stewart 
            K, Szelenyi E, Tieu M, Trader C, Tran A, van Velthoven CTJ, Walker 
            M, Wang Y, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T, 
            Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith
            KA, Esposito L, Waters J, Thyagarajan B, Yao S, Lein ES, Zeng H, 
            Levi BP, Ngai J, Ting JT, Tasic B
  TITLE     A suite of enhancer AAVs and transgenic mouse lines for genetic 
            access to cortical cell types.
  JOURNAL   Cell. 2025 May 16:S0092-8674(25)00513-6. doi: 
            10.1016/j.cell.2025.05.002.
  PUBMED    40403729
REFERENCE   2  (bases 1 to 5127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 16:S0092-8674(25)00513-6. doi: 10.1016/j.cell.2025.05.002."
COMMENT     Sequence Label: AiP1302 - pAAV-AiE2013m-minBG-iCre-HGHpA
FEATURES             Location/Qualifiers
     source          1..5127
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    48..524
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(247..266)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   564..704
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   564..693
                     /label=AAV2 ITR
     rep_origin      779..1234
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(866..885)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1076..1097
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1251..1270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1370..1392
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1430..1448)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1516..1620
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1621..1689,1690..2481)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1839..1858)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2652..3240
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3141..3160
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   3302..3431
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      4094..4103
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4106..4126
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggtgaggctg ctcgaggatg gggactaagg ctgctcgaga gatctacggg tggcatccct
       61 gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc
      121 ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta
      181 tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc tgtagggcct
      241 gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc actgcaatct
      301 ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg ggattccagg
      361 catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat
      421 tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg cctcccaaat
      481 tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt ttgtaggtaa
      541 ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct
      601 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
      661 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta
      721 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
      781 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
      841 acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
      901 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
      961 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
     1021 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     1081 ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa
     1141 gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     1201 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
     1261 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     1321 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     1381 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     1441 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     1501 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     1561 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     1621 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     1681 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     1741 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     1801 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     1861 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     1921 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     1981 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     2041 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     2101 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     2161 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     2221 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     2281 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     2341 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     2401 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     2461 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     2521 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     2581 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     2641 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2701 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2761 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     2821 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2881 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2941 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3001 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3061 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3121 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3181 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3241 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3301 tcctgcaggc agctgcgcgc tcgctcgctc actgaggccg cccgggcgtc gggcgacctt
     3361 tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac
     3421 taggggttcc tgcggccgca cgcgtgtccc ataggcagtt tgtggctgag tgctggtcca
     3481 ggggtgagga ggtgggctat gttactgagg gtctctgggt gttaggaaaa cagggcccag
     3541 gagtctggct gctcgtatgc tggcccaggc tcttgttttt cttgagctga cttgctggag
     3601 aagtgagcta agtcagaaac aaaatgccac attgcacgcc cactgaagtc tgggctcaag
     3661 ggaaagaaga gagattgcca gagcgttagc tgttcccaat ccactcctgg accttaagct
     3721 gtcttgaaca gagttgccaa tcagcttggt agggactggc ctttgaggag gggagggggt
     3781 gtaggcaggg gagggggaga gaagggagca gtctgcgctc catcttaatt acctcatcag
     3841 aaacagctcc cttcccgcaa agctctggtg tcttctacaa gagggtgagt ctttggcttt
     3901 acatgtgaac ttgtgccatt tgcctgcgta tataaacatg aagggtcgtc tgggttcaga
     3961 gctgaaatct ttcacttgtg acttagctgg gaaattcttg gcaagatcag aatgcagtgg
     4021 taaggtctga gctcgggctg ggcataaaag tcagggcaga gccatctatt gcttacattt
     4081 gcttctggga tccgccacca tggtgcccaa gaagaagagg aaagtctcca acctgctgac
     4141 tgtgcaccaa aacctgcctg ccctccctgt ggatgccacc tctgatgaag tcaggaagaa
     4201 cctgatggac atgttcaggg acaggcaggc cttctctgaa cacacctgga agatgctcct
     4261 gtctgtgtgc agatcctggg ctgcctggtg caagctgaac aacaggaaat ggttccctgc
     4321 tgaacctgag gatgtgaggg actacctcct gtacctgcaa gccagaggcc tggctgtgaa
     4381 gaccatccaa cagcacctgg gccagctcaa catgctgcac aggagatctg gcctgcctcg
     4441 cccttctgac tccaatgctg tgtccctggt gatgaggaga atcagaaagg agaatgtgga
     4501 tgctggggag agagccaagc aggccctggc ctttgaacgc actgactttg accaagtcag
     4561 atccctgatg gagaactctg acagatgcca ggacatcagg aacctggcct tcctgggcat
     4621 tgcctacaac accctgctgc gcattgccga aattgccaga atcagagtga aggacatctc
     4681 ccgcaccgat ggtgggagaa tgctgatcca cattggcagg accaagaccc tggtgtccac
     4741 agctggtgtg gagaaggccc tgtccctggg ggttaccaag ctggtggaga gatggatctc
     4801 tgtgtctggt gtggctgatg accccaacaa ctacctgttc tgccgggtca gaaagaatgg
     4861 tgtggctgcc ccttctgcca cctcccaact gtccacccgg gccctggaag ggatctttga
     4921 ggccacccac cgcctgatct atggtgccaa ggatgactct gggcagagat acctggcctg
     4981 gtctggccac tctgccagag tgggtgctgc cagggacatg gccagggctg gtgtgtccat
     5041 ccctgaaatc atgcaggctg gtggctggac caatgtgaac attgtgatga actacatcag
     5101 aaacctggac tctgagactg gggccat
//
