LOCUS       sequence-46629-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Onder TT, Kara N, Cherry A, Sinha AU, Zhu N, Bernt KM, Cahan P, 
            Marcarci BO, Unternaehrer J, Gupta PB, Lander ES, Armstrong SA, 
            Daley GQ
  TITLE     Chromatin-modifying enzymes as modulators of reprogramming.
  JOURNAL   Nature. 2012 Mar 4;483(7391):598-602. doi: 10.1038/nature10953.
  PUBMED    22388813
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-03-4- 4"; volume: "483"; issue: "7391"; pages:
            "598-602"
COMMENT     Sequence Label: pSMP-DNMT3A_1
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     57..79
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     95..111
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     ncRNA           197..211
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           239..365
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(460..479)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(461..479)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(533..556)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 agccctttgt caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
       61 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
      121 aggcgccgga attagatctc tcgagaaggt atattgctgt tgacagtgag cgcgcccaag
      181 gtcaaggaga ttattagtga agccacagat gtaataatct ccttgacctt gggcttgcct
      241 actgcctcgg aattcaaggg gctactttag gagcaattat cttgtttact aaaactgaat
      301 accttgctat ctctttgata catttttaca aagctgaatt aaaatggtat aaattaaatc
      361 acttttttca attggaagac taatgcggcc ggccatatat atcgttatat ggtggggagc
      421 tgtggcttga aatttgtttc actttaggcg ctggttgggc cctatagtga gtcgtattac
      481 ctaggacgcg ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc
      541 tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc
      601 cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc
      661 tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg
      721 acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg
//
