LOCUS       sequence-46632-e         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Onder TT, Kara N, Cherry A, Sinha AU, Zhu N, Bernt KM, Cahan P, 
            Marcarci BO, Unternaehrer J, Gupta PB, Lander ES, Armstrong SA, 
            Daley GQ
  TITLE     Chromatin-modifying enzymes as modulators of reprogramming.
  JOURNAL   Nature. 2012 Mar 4;483(7391):598-602. doi: 10.1038/nature10953.
  PUBMED    22388813
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-03-4- 4"; volume: "483"; issue: "7391"; pages:
            "598-602"
COMMENT     Sequence Label: pSMP-DNMT1_1
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     88..104
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     ncRNA           190..204
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           232..358
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(451..470)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(452..470)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(524..547)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 gtcaccctaa gcctccgcct cctcttcctc catccgcccc gtctctcccc cgttgaacct
       61 cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc tctaggcgcc
      121 ggaattagat ctctcgagaa ggtatattgc tgttgacagt gagcgcgcct gcaaggacat
      181 ggttaaatag tgaagccaca gatgtattta accatgtcct tgcaggcttg cctactgcct
      241 cggaattcaa ggggctactt taggagcaat tatcttgttt actaaaactg aataccttgc
      301 tatctctttg atacattttt acaaagctga attaaaatgg tataaattaa atcacttttt
      361 tcaattggaa gactaatgcg gccggccagg gctacaattc tctgacgagg ggcgcagtgc
      421 ttcttcgcct tttggagttg gattttaggg ccctatagtg agtcgtatta cctaggacgc
      481 gttctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc
      541 cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac
      601 cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc
      661 cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga
      721 agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt
      781 aggccttt
//
