LOCUS       sequence-46633-e         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Onder TT, Kara N, Cherry A, Sinha AU, Zhu N, Bernt KM, Cahan P, 
            Marcarci BO, Unternaehrer J, Gupta PB, Lander ES, Armstrong SA, 
            Daley GQ
  TITLE     Chromatin-modifying enzymes as modulators of reprogramming.
  JOURNAL   Nature. 2012 Mar 4;483(7391):598-602. doi: 10.1038/nature10953.
  PUBMED    22388813
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-03-4- 4"; volume: "483"; issue: "7391"; pages:
            "598-602"
COMMENT     Sequence Label: pSMP-DNMT1_2
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..78
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     94..110
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     ncRNA           196..210
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           238..364
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(458..477)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(459..477)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(531..554)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 agccctttgt cccctaagcc tccgcctcct cttcctccat ccgccccgtc tctccccctt
       61 gaacctcctc gttcgacccc gcctcgatcc tccctttatc cagccctcac tccttctcta
      121 ggcgccggaa ttagatctct cgagaaggta tattgctgtt gacagtgaac gacgcaccta
      181 ctccaagttc aaatagtgaa gccacagatg tatttgaact tggagtaggt gcgctgccta
      241 ctgcctcgga attcaagggg ctactttagg agcaattatc ttgtttacta aaactgaata
      301 ccttgctatc tctttgatac atttttacaa agctgaatta aaatggtata aattaaatca
      361 cttttttcaa ttggaagact aatgcggccg gccacgttgg ggcagacttg gttgtcttca
      421 catgttgagt ggggtgtttt gggtaatagg acttgggccc tatagtgagt cgtattacct
      481 aggacgcgtt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt
      541 tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca
      601 catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta
      661 ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac
      721 aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga
      781 agcgggtagg cctttggggc agcggccaaa tagcagc
//
