LOCUS       sequence-46634-e         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Onder TT, Kara N, Cherry A, Sinha AU, Zhu N, Bernt KM, Cahan P, 
            Marcarci BO, Unternaehrer J, Gupta PB, Lander ES, Armstrong SA, 
            Daley GQ
  TITLE     Chromatin-modifying enzymes as modulators of reprogramming.
  JOURNAL   Nature. 2012 Mar 4;483(7391):598-602. doi: 10.1038/nature10953.
  PUBMED    22388813
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-03-4- 4"; volume: "483"; issue: "7391"; pages:
            "598-602"
COMMENT     Sequence Label: pSMP-DNMT1_3
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     40..62
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     78..94
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     ncRNA           180..194
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           222..348
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(442..461)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(443..461)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(515..538)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 agcctccgcc tcctcttcct ccatccgccc cgtctctccc ccttgaacct cctcgttcga
       61 ccccgcctcg atcctccctt tatccagccc tcactccttc tctaggcgcc ggaattagat
      121 ctctcgagaa ggtatattgc tgttgacagt gagcgccgag tctggctttg agagttatag
      181 tgaagccaca gatgtataac tctcaaagcc agactcgttg cctactgcct cggaattcaa
      241 ggggctactt taggagcaat tatcttgttt actaaaactg aataccttgc tatctctttg
      301 atacattttt acaaagctga attaaaatgg tataaattaa atcacttttt tcaattggaa
      361 gactaatgcg gccggccatc tatggcgtac tttatctccg cgtggatctt ttgttccttc
      421 gtttcttgta ggtataaggg gccctatagt gagtcgtatt acctaggacg cgttctaccg
      481 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
      541 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
      601 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
      661 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
      721 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
      781 gggcagcggc caatagcagc tttgctcctt cgc
//
