LOCUS       sequence-466380-        9278 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Optimized VEGF-sensing eLIDAR with VEGFR1 ecto+TM domains fused with
            ADAR2dd, with Golgi traffic sequence and ER export sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9278)
  AUTHORS   Zhang X, Mille-Fragoso LS, Kaseniit KE, Lee AP, Zhang M, Call CC, Hu
            Y, Xie Y, Gao XJ
  TITLE     Post-transcriptional modular synthetic receptors.
  JOURNAL   Nat Chem Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w.
  PUBMED    40155716
REFERENCE   2  (bases 1 to 9278)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w."
COMMENT     Sequence Label: XZ363
            EF1a-VEGFR1sp-3xFlag-VEGFR1(ecto+TM)-TS-...
FEATURES             Location/Qualifiers
     source          1..9278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     322..341
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        416..520
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(521..589,590..1381)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(739..758)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1552..2140
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2041..2060
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2294..2311
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2428..2449
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2464..2469,2470..2487,2488..2494)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2502..2518
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2507..2529
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2526..2542
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2526..2542
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    2591..2712
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2602..2621)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2656..2675
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2842..3864)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             complement(2842..3864)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     protein_bind    complement(3872..3919)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     4051..4070
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    4242..4466
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     4455..4472
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(4528..4557)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(8152..8217)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      8292..8301
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          8323..9261
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(8346..8366)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        complement(join(9262..9278,1..195))
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 acccgttgcg aaaaagaacg ttcacggcga ctactgcact tatatacggt tctcccccac
       61 cctcgggaaa aaggcggagc cagtacacga catcactttc ccagtttacc ccgcgccacc
      121 ttctctaggc accggttcaa ttgccgaccc ctccccccaa cttctcgggg actgtgggcg
      181 atgtgcgctc tgcccactga cgggcacgat tcttcatgca attgtcggtc aagccttgcc
      241 ttgttgtagc ttaaattttg ctcgcgcact actcagcgac ctccaacaca caagcaggga
      301 gcagatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccataggg
      361 gatcgggaga tctcccgatc cgtcgacgtc aggtggcact tttcggggaa atgtgcgcgg
      421 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
      481 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
      541 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
      601 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
      661 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
      721 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
      781 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
      841 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
      901 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
      961 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     1021 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     1081 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     1141 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     1201 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     1261 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     1321 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     1381 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     1441 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     1501 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     1561 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     1621 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     1681 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     1741 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     1801 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     1861 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     1921 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     1981 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     2041 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     2101 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     2161 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     2221 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     2281 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     2341 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     2401 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     2461 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     2521 tcacacagga aacagctatg accatgatta cgccaagctc tagctagagg tcgacggtat
     2581 acagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     2641 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     2701 aataaacaag ttggggtggg cgaagaactc cagcatgaga tccccgcgct ggaggatcat
     2761 ccagccggcg tcccggaaaa cgattccgaa gcccaacctt tcatagaagg cggcggtgga
     2821 atcgaaatct cgtagtacgt gctattcctt tgccctcgga cgagtgctgg ggcgtcggtt
     2881 tccactatcg gcgagtactt ctacacagcc atcggtccag acggccgcgc ttctgcgggc
     2941 gatttgtgta cgcccgacag tcccggctcc ggatcggacg attgcgtcgc atcgaccctg
     3001 cgcccaagct gcatcatcga aattgccgtc aaccaagctc tgatagagtt ggtcaagacc
     3061 aatgcggagc atatacgccc ggagccgcgg cgatcctgca agctccggat gcctccgctc
     3121 gaagtagcgc gtctgctgct ccatacaagc caaccacggc ctccagaaga agatgttggc
     3181 gacctcgtat tgggaatccc cgaacatcgc ctcgctccag tcaatgaccg ctgttatgcg
     3241 gccattgtcc gtcaggacat tgttggagcc gaaatccgcg tgcacgaggt gccggacttc
     3301 ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc tgcgcgacgg acgcactgac
     3361 ggtgtcgtcc atcacagttt gccagtgata cacatgggga tcagcaatcg cgcatatgaa
     3421 atcacgccat gtagtgtatt gaccgattcc ttgcggtccg aatgggccga acccgctcgt
     3481 ctggctaaga tcggccgcag cgatcgcatc catggcctcc gcgaccggct gcagaacagc
     3541 gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc tgtgcacggc gggagatgca
     3601 ataggtcagg ctctcgctga attccccaat gtcaagcact tccggaatcg ggagcgcggc
     3661 cgatgcaaag tgccgataaa cataacgatc tttgtagaaa ccatcggcgc agctatttac
     3721 ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa gcacgagatt cttcgccctc
     3781 cgagagctgc atcaggtcgg agacgctgtc gaacttttcg atcagaaact tctcgacaga
     3841 cgtcgcggtg agttcaggct ttttggccaa ggaagttcct atactttcta gagaatagga
     3901 acttcggaat aggaacttct aggtacgtga accatcaccc taatcaagtt ttttggggtc
     3961 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     4021 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     4081 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     4141 gccgctacag ggcgcgtggg gataccccct agagccccag ctggttcttt ccgcctcaga
     4201 agccatagag cccaccgcat ccccagaaca tcgatagatc tccatagagc ccaccgcatc
     4261 cccagcatgc ctgctattgt cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc
     4321 ccccagaata gaatgacacc tactcagaca atgcgatgca atttcctcat tttattagga
     4381 aaggacagtg ggagtggcac cttccagggt caaggaaggc acgggggagg ggcaaacaac
     4441 agatggctgg caactagaag gcacagtcga ggctgatcag cgggtttaaa ctcaatgatg
     4501 attatgatga tggtcgacgg cgctattcag atcctcttct gagatgagtt tttgttcggg
     4561 ccctcctcga gcggctcaca cctcgttctc gtagcagaag ggcgtgagtg agaactggtc
     4621 ctgctcggtg ggcttctcca cccaggcccc cagccccgcc ttgatgaagg ctgtgaacag
     4681 acgcgccttg gcggcctggt actcctttgc cgccagcttg gactcatggt acacgttggg
     4741 tttggtaatc ttggagcgta gtaagtggga gggaaccttg ccgtgcacac gcatccagcg
     4801 acagtacaac gcgtgcttac acaggcggga cgcgcggccc agctcatcct tcccagtcgt
     4861 ggcgttgatg acctcaatag cggagtcgcc taccgtccag ttgacactga agttgggggc
     4921 cttccctggc tgccgtgctt ctgcattgct gatgccactg agcaaaggct tgttgagggt
     4981 gtagagaggt ggcaggtcct ctatgttgga gatccgctgg tacatggccc tggaaaggtg
     5041 gtccccgtgg taaaggctgc ccaggatgat gctcgagaag taaatgggct ccacgaaaat
     5101 gctgagcagg gatccctgga tgcccaccac gttccagcgt gcaatcttgt cactgcagga
     5161 catggtgagc agccgctccc cttgcagcac cccgtcccac gcttggatgc tcgcattgga
     5221 gcgcactgga atcgtcccct gaccagactc tattttggtc cgtagctgtc ctcttgcttt
     5281 acgatttggg tgtctatctg ctggttcttc caggattggc tcatgtggtg agaagattct
     5341 ggcatctcca cagggagagg tgctgatgta cagatgaaac tggacattct ccttcagcct
     5401 aaacccccct cgctctgatt tctgaaagat ggatcttttt tgatcatctt tgttatttaa
     5461 gtaaagctca agttgtgtat aaagaaatct gagcaaggat ctccgagata ttatttctgc
     5521 atggcagtca tttaatgcaa ggccacgatc actcatgtat tcaccattaa tacattttgt
     5581 tcctgtagaa acacttatca ccttggcatc tttaacatct gtgcctgttg tcatgacgac
     5641 tccagccagc acttttctgc gagcgtgagg ggaggagaag ttgtcggtca ggtcaccaaa
     5701 cttacccagg accaggcgtg agacagcgtc agctaaaacc tgcggtaaat gcagctgaag
     5761 accctcactg ggaataggct ggcgagatgg cgtttgatcc aagtgcaaca cgttgatgtc
     5821 gatctggtcc agggggatgt actcgccctc gctggtgatc ctgctcttgg cggccgccct
     5881 caggataaag agggttaata ggagccagaa gagagtcgca gccacacagg tgcatgttag
     5941 agtgatcagc tccagattac tcttgtccga ggttccttga acagtgaggt atgctgaact
     6001 ttccacagag cccttctggt tggtggcttt gcagtgatag acaccttcat cctcttctgt
     6061 gactctttca ataaacagcg tgctgcttcc tggtcctaaa ataattccag gctcttgttg
     6121 tattttgtgg ttgtttttaa accaagtgat ctgaggctcg gggacaccat tagcatgaca
     6181 gtctaaagtg gtggaactgc tgatggccac tgtgtgatca ctgaggtttc gcaggaggta
     6241 tggtgcttcc tgatctctga ttgtaatttc tttcttctgg aggatttctt cccctgtgta
     6301 tacattcctg gctctgcagg cataggtgcc tgaatcttgc agggaaacat tcatgatggt
     6361 aagattaaga gtgatggagt gctccttagt gatggccatt ttttgcttgc taatactgta
     6421 gtgcattgtt ctgttattaa ctgtccgcag taaaatccaa gtaacgtctc tgtataagaa
     6481 cttgttaact gtgcaagaca gtttcaggtc ctctccttcc gtcggcattt tttccaagtt
     6541 aacatgaaac ccatttggca catctgtgat ataaaagctt atgtttcttc ccacagtccc
     6601 aactttattg gaagctatgc aaatgtagat tccagaaatt ctagagtcag ccacaaccaa
     6661 ggtgctagcc atcttattct ttccttctat tattgccatg cgctgagtga tgctctcaat
     6721 tctgtttccc atgttgctgt cagcatccag gataaaggac tcttcattat tggaacaaaa
     6781 gtcacacctt gcttcggaat gattatggtt acaggggtgc cagaaccact tgattgtagg
     6841 ttgagggata ccatatgcgg tacaagtcag gatttgtctg ctgcccagtg ggtagagagc
     6901 cgggtctgga aacgatgaca cagccttttc gtaaatctgg ggtttcacat tgacaattag
     6961 agtggcagtg aggtttttaa acacatttga ctgttttatg ctcagcaaga ttgtataatt
     7021 ccctgcatcc tcttcagtta cgtccttgat aattaacgag tagccacgag tcaaatagcg
     7081 agcagatttc tcagtcgcag gtaacccatc ttttaaccat acaacttccg gcgagggaaa
     7141 tgccttcact ttcatagaga gccggtaaga ccgcttgcca gctacggttt caagcacctg
     7201 ctgttttcga tgtttcacag tgatgaatgc tttatcatat atatgcactg aggtgttaac
     7261 agatttgaat gatggtccac tccttacacg acaagtataa agtcctttgt ctttgttctg
     7321 cattttgtca atagtaagaa cactgtagaa tatgttggca tgggaattgc tttggtcaat
     7381 tcgtcgcctt acggaagctc tcttattttt ttcatcaggg taactccagg tcatttgaac
     7441 tctcgtgttc aagggagtgg tagcagtaca attgagaaca agagtatggc ctctaagtaa
     7501 tttgactggg cgtggtgtgc ttatttggac atctatgatt gtattggttt gtcgatgtgt
     7561 gagatagttt gtcttataca aatgcccatt gactgttgct tcacaggtca gaagccctat
     7621 ttctttgtac gttgcatttg atatgatgaa gccctttcta ctgtcccaga ttatgcgttt
     7681 tccatcaggg atcaaagtgt caagtggaaa cttttttaaa gtaacagtga tgttaggtga
     7741 cgtaacccgg cagggaatga cgagctccct tccttcagtc atgtgtataa tttcggggat
     7801 ttcactgtac atctctacga aaggtctacc tgtatcacta ataaatatat agattgcaga
     7861 ttctgtttcc ttcttctttg aagtaggtac agctagatat ttgcagctgt agaagccagt
     7921 gtggtttgct tgagctgtgt tcaaggttaa agtactgcag aattgtttgc catttcttcc
     7981 acaggcagat ttagttatgc tcagcctttc gctttcctta ctcaccattt caggcaaaga
     8041 ccatttatgg gctgcttccc ccctgcattg gagatgcagt gtctggcctg cttgcatgat
     8101 gtgctgggtg ccttttaaac tcagttcagg atcttttaat tttgaggtac ccttatcatc
     8161 gtcatccttg tagtcaatgt catgatcttt gtaatcccca tcgtgatcct tgtagtcacc
     8221 tgaactagat cctgtgagaa gcagacagct gagcagcgcg cacagcagga ccccggtgtc
     8281 ccagtagctg accatggtgg cgaactagta gcttcacgac acctgaaatg gaagaaaaaa
     8341 actttgaacc actgtctgag gcttgagaat gaaccaagat ccaaactcaa aaagggcaaa
     8401 ttccaaggag aattacatca agtgccaagc tggcctaact tcagtctcca cccactcagt
     8461 gtggggaaac tccatcgcat aaaacccctc cccccaacct aaagacgacg tactccaaaa
     8521 gcgcgagaac taatcgaggt gcctggacgg cgcccggtac tccgtggagt cacatgaagc
     8581 gacggctgag gacggaaagg cccttttcct ttgtgtgggt gactcacccg cccgctctcc
     8641 cgagcgccgc gtcctccatt ttgagctccc tgcagcaggg ccgggaagcg gccatctttc
     8701 cgctcacgca actggtgccg accgggccag ccttgccgcc cagggcgggg cgatacacgg
     8761 cggcgcgagg ccaggcacca gagcaggccg gccagcttga gactaccccc gtccgattct
     8821 cggtggccgc gctcgcaggc cccgcctcgc cgaacatgtg cgctgggacg cacgggcccc
     8881 gtcgccgccc gcggccccaa aaaccgaaat accagtgtgc agatcttggc ccgcatttac
     8941 aagactatct tgccagaaaa aaagcgtcgc agcaggtcat caaaaatttt aaatggctag
     9001 agacttatcg aaagcagcga gacaggcgcg aaggtgccac cagattcgca cgcggcggcc
     9061 ccagcgccca ggccaggcct caactcaagc acgaggcgaa ggggctcctt aagcgcaagg
     9121 cctcgaactc tcccacccac ttccaacccg aagctcggga tcaagaatca cgtactgcag
     9181 ccaggtggaa gtaattcaag gcacgcaagg gccataaccc gtaaagaggc caggcccgcg
     9241 ggaaccacac acggcactta cctgtgttct ggcggcaa
//
