LOCUS       sequence-466381-        7021 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AC5 promoter driven FRBP-ADAR2dd for drosophila cell expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7021)
  AUTHORS   Zhang X, Mille-Fragoso LS, Kaseniit KE, Lee AP, Zhang M, Call CC, Hu
            Y, Xie Y, Gao XJ
  TITLE     Post-transcriptional modular synthetic receptors.
  JOURNAL   Nat Chem Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w.
  PUBMED    40155716
REFERENCE   2  (bases 1 to 7021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w."
COMMENT     Sequence Label: APL0040 Ac5-FKBP-ADARdd
FEATURES             Location/Qualifiers
     source          1..7021
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1882..1891
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1897..1899,1900..2220)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             3591..3632
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3642..3659
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3682..3699)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3732..3866
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3756..3775)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3810..3829
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(4467..5055)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4547..4566)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5226..6017,6018..6086))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5849..5868
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6087..6191)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6259..6277
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(6341..7021,1..1829)
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
ORIGIN
        1 ccagaccaaa aggcttgaat ttctgcgtct tttgccgaac gcagtgcatg tgcaattgtt
       61 gttttttgga atattcaatt ttcggactgt ccgctttgat ttcagtttct tggcttattc
      121 aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca aaacggtagt
      181 ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg cggggtctgt
      241 ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg cgtgtgtgta
      301 ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatgctgcgc tctctctagc
      361 gccatctcgc ttacgcatgc tcaacgcacc gcatgttgcc gtttcctttt atgcgtcatt
      421 ttggctcgaa ataggcaatt atttaaacaa agattagtca acgaaaacgc taaaataaat
      481 aagtctacaa tatggttact tattgccatg tgtgtgcagc caacgatagc aacaaaagca
      541 acaacacagg tggctttccc tctttcactt tttgtttgca agccgcgtgc gagcaagacg
      601 gcacgaccgg caaacgcaat tacgctgaca aagagcagac gaagttttgg cgaaaaacat
      661 caaggcgcct gatacgaatg catttgcaat aacaattgcg atatttaata ttgtttatga
      721 agctgtttga cttcaaaaca cacaaaaaaa aaaataaaac aaattatttg aaagagaatt
      781 aggaatcgga cgcttatcgt tagggtaaca acaagaaatg cttactgagt cacagcctct
      841 ggaaaactgc cgcaagccag agagagagag aaaaagaggg agagcagctt agaccgcatg
      901 tgcttgtgtg tgaggcgtct ctctcttcgt ctctgttgcg caaacgcata gactgcactg
      961 aaaaaatcga ttacctattt tttatgaatg aatatttgca ctattactat tcaaaactat
     1021 taagatagca atcacattca atagccaaat actataccac ctgagcgatg caacgaaatg
     1081 atcaatttga gcaaaaatgc tgcatattta ggacggcatc attatagaaa tgcttcttgc
     1141 tgtgtacttt tctctcgtct ggcagctgtt tcgccgttat tgttaaaacc ggcttaagtt
     1201 aggtgtgttt tctacgacta gtgaatgccc tactagaaga tgtgtgttgc acaaaatgtc
     1261 cctggaataa ccaatttgaa gtgcagatag cagtaaacgt aagctaatat gaatattatt
     1321 taactgtaat gttttaatat cgctggacat tactaataaa cccactataa acacatgtac
     1381 atatgtatgt tttggcatac aatgagtagt tggggaaaaa atgtgtaaaa gcaccgtgac
     1441 catcacagca taaagataac cagctgaagt atcgaatatg agtaaccccc aaattgaatc
     1501 acatgccgca actgatagga cccatggaag tacactcttc atggcgatat acaagacaca
     1561 cacaagcacg aacacccagt tgcggaggaa attctccgta aatgaaaacc caatcggcga
     1621 acaattcata cccatatatg gtaaaagttt tgaacgcgac ttgagagcgg agagcattgc
     1681 ggctgataag gttttagcgc taagcgggct ttataaaacg ggctgcggga ccagttttca
     1741 tatcactacc gtttgagttc ttgtgctgtg tggatactcc tcccgacaca aagccgctcc
     1801 atcagccagc agtcgtctaa tccagagacc ccggatcggg gtacctacta gtccagtgtg
     1861 gtggaattcc gaaatgggat cgccaccatg gctagcatgg gagtgcaggt ggaaaccatc
     1921 tccccaggag acgggcgcac cttccccaag cgcggccaga cctgcgtggt gcactacacc
     1981 gggatgcttg aagatggaaa gaaatttgat tcctcccggg acagaaacaa gccctttaag
     2041 tttatgctag gcaagcagga ggtgatccga ggctgggaag aaggggttgc ccagatgagt
     2101 gtgggtcaga gagccaaact gactatatct ccagattatg cctatggtgc cactgggcac
     2161 ccaggcatca tcccaccaca tgccactctc gtcttcgatg tggagcttct aaaactggaa
     2221 ggcgccggga caggagggtc tgggggtggc actggcggat ctggaggaga ctacaaggac
     2281 gatgacgaca acggcggatc gggggggtct ggtggtagtg gcggttcggg gggaggtacc
     2341 ggtttgcact tggatcaaac gccatctcgc cagcctattc ccagtgaggg tcttcagctg
     2401 catttaccgc aggttttagc tgacgctgtc tcacgcctgg tcctgggtaa gtttggtgac
     2461 ctgaccgaca acttctcctc ccctcacgct cgcagaaaag tgctggctgg agtcgtcatg
     2521 acaacaggca cagatgttaa agatgccaag gtgataagtg tttctacagg aacaaaatgt
     2581 attaatggtg aatacatgag tgatcgtggc cttgcattaa atgactgcca tgcagaaata
     2641 atatctcgga gatccttgct cagatttctt tatacacaac ttgagcttta cttaaataac
     2701 aaagatgatc aaaaaagatc catctttcag aaatcagagc gaggggggtt taggctgaag
     2761 gagaatgtcc agtttcatct gtacatcagc acctctccct gtggagatgc cagaatcttc
     2821 tcaccacatg agccaatcct ggaagaacca gcagatagac acccaaatcg taaagcaaga
     2881 ggacagctac ggaccaaaat agagtctggt caggggacga ttccagtgcg ctccaatgcg
     2941 agcatccaag cgtgggacgg ggtgctgcaa ggggagcggc tgctcaccat gtcctgcagt
     3001 gacaagattg cacgctggaa cgtggtgggc atccagggat ccctgctcag cattttcgtg
     3061 gagcccattt acttctcgag catcatcctg ggcagccttt accacgggga ccacctttcc
     3121 agggccatgt accagcggat ctccaacata gaggacctgc cacctctcta caccctcaac
     3181 aagcctttgc tcagtggcat cagcaatgca gaagcacggc agccagggaa ggcccccaac
     3241 ttcagtgtca actggacggt aggcgactcc gctattgagg tcatcaacgc cacgactggg
     3301 aaggatgagc tgggccgcgc gtcccgcctg tgtaagcacg cgttgtactg tcgctggatg
     3361 cgtgtgcacg gcaaggttcc ctcccactta ctacgctcca agattaccaa acccaacgtg
     3421 taccatgagt ccaagctggc ggcaaaggag taccaggccg ccaaggcgcg tctgttcaca
     3481 gccttcatca aggcggggct gggggcctgg gtggagaagc ccaccgagca ggaccagttc
     3541 tcactcacgc cctgagcggc cgctcgagtc tagagggccc gcggttcgaa ggtaagccta
     3601 tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg tcatcatcac catcaccatt
     3661 gagtttaaac ccgctgatca gcctcgactg tgccttctaa ggcctgagct cgctgatcag
     3721 cctcgatcga ggatccagac atgataagat acattgatga gtttggacaa accacaacta
     3781 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     3841 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     3901 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg
     3961 ctgattatga tcagtcgacc gatgcccttg agagccttca acccagtcag ctccttccgg
     4021 tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc
     4081 gtaggacagg tgccggcagc gctctgggtc attttcggcg aggaccgctt tcgctggagc
     4141 gcgacgatga tcggcctgtc gcttgcggta ttcggaatct tgcacgccct cgctcaagcc
     4201 ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc aggccattat cgccggcatg
     4261 gcggccgacg cgctgggcta cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc
     4321 cccattatga ttcttctcgc ttccggcggc atcgggatgc ccgcgttgca ggccatgctg
     4381 tccaggcagg tagatgacga ccatcaggga cagcttcaag gccagcaaaa ggccaggaac
     4441 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     4501 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     4561 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     4621 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     4681 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     4741 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     4801 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     4861 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     4921 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     4981 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     5041 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     5101 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     5161 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     5221 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     5281 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     5341 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     5401 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     5461 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     5521 cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     5581 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     5641 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     5701 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     5761 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     5821 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa
     5881 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     5941 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     6001 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     6061 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     6121 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     6181 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     6241 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattaatt
     6301 ctatattcta aaaacacaaa tgatacttct aaaaaaaaat catgaatggc atcaactctg
     6361 aatcaaatct ttgcagatgc acctacttct catttccact gtcacatcat ttttccagat
     6421 ctcgctgcct gttatgtggc ccacaaacca agacacgttt tatggccatt aaagctggct
     6481 gatcgtcgcc aaacaccaaa tacataatga atatgtacac attcgagaaa gaagcgatca
     6541 aagaagcgtc ttcgggcgga gtaggagaat gcggaggaga aggagaacga gctgatctag
     6601 tatctctcca caatccaatg ccaactgacc aactggccat attcggagca atttgaagcc
     6661 aatttccatc gcctggcgat cgctccattc ttggctatat gtttttcacc gttacccggg
     6721 gccattttca aagactcgtc ggcaagataa gattgtgtca ctcgctgtct ctcttcattt
     6781 gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag aatgagaata
     6841 atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt tatctataga
     6901 taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac ctttaatgta
     6961 ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt ttaagatgat
     7021 g
//
