LOCUS       sequence-466385-        7729 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  human oxytocin receptor-V2-ADAR2dd(E488Q, T501A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7729)
  AUTHORS   Zhang X, Mille-Fragoso LS, Kaseniit KE, Lee AP, Zhang M, Call CC, Hu
            Y, Xie Y, Gao XJ
  TITLE     Post-transcriptional modular synthetic receptors.
  JOURNAL   Nat Chem Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w.
  PUBMED    40155716
REFERENCE   2  (bases 1 to 7729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w."
COMMENT     Sequence Label: XZ105 CMV-TO-signal-hOXTR-V2-ADAR2dd(DD-E488Q-...
FEATURES             Location/Qualifiers
     source          1..7729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(108..212)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(287..306)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        473..852
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        853..1056
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1007..1027
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    1058..1076
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1079..1097
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1107..1131
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3800..3829
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(3885..3902)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3891..4115
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4287..4306)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4438..4485
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4493..5515
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4493..5515
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    5645..5766
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5682..5701)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5736..5755
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5815..5831)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5815..5831)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5828..5850)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5839..5855
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5863..5869,5870..5887,5888..5893))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5908..5929
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6046..6063)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6217..6805)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6297..6316)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6976..7729,1..38,39..107))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7599..7618
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
       61 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
      121 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
      181 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
      241 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
      301 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      361 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      421 tgcttagggt taggcgtttt gcgctgcttc gtacgggcca gatatacgcg ttgacattga
      481 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
      541 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
      601 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      661 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      721 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      781 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
      841 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
      901 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
      961 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     1021 aggcgtgtac ggtgggaggt ctatataagc agagctctcc ctatcagtga tagagatctc
     1081 cctatcagtg atagagatcg tcgacgagct cgtttagtga accgtcagat cgcctggaga
     1141 cgccatccac gctgttttga cctccataga agacaccggg accgatccag cctccggact
     1201 ctagcgttta aacttaagct tcgccaccat gaagacgatc atcgccctga gctacatctt
     1261 ctgcctggta ttcgccgact acaaggacga tgatgacgcc agcggcgaag gggcccttgc
     1321 cgccaattgg tctgccgagg cggcgaacgc tagtgcggcc ccccctggcg cggaaggtaa
     1381 ccgaacggca ggcccaccta ggagaaatga agctttggcg agagtggagg tggccgtcct
     1441 ctgccttatt ctgctgttgg ccttgtccgg aaacgcttgt gtgctgctcg cgctccgcac
     1501 gacccggcag aagcactcca gactgttttt ttttatgaag caccttagca tcgctgacct
     1561 cgtcgttgcg gtattccagg tgctgcccca actgctgtgg gatatcacat tcaggtttta
     1621 cggcccagac ttgctctgta gacttgtcaa gtacctccag gttgtcggaa tgttcgcgtc
     1681 cacatacctc cttcttttga tgtcactgga ccgatgtctg gcaatatgtc agccccttag
     1741 gagcctgcgg agaagaacgg acaggctcgc tgtactcgct acatggctcg gctgtcttgt
     1801 ggcatccgca cctcaggttc atatcttttc actcagagaa gtggcagatg gcgtgtttga
     1861 ctgttgggcc gtattcattc agccatgggg acccaaggcg tatatcacct ggatcacgtt
     1921 ggctgtgtat atcgttcctg ttatcgtgtt ggccgcttgc tacggcctca tctcattcaa
     1981 aatttggcag aacctccgct tgaaaacagc tgccgctgct gctgcagaag ctccagaagg
     2041 cgcggcagct ggcgacggtg gacgggttgc actcgccagg gtcagtagcg ttaagctcat
     2101 cagcaaggca aagatccgga ctgttaaaat gacctttatt attgtgcttg cttttattgt
     2161 gtgttggact cccttcttct ttgtacagat gtggagcgtg tgggacgcta acgcccccaa
     2221 ggaggcgagc gcgttcatta tagtgatgtt gctcgcgtcc ctgaattcct gttgtaaccc
     2281 gtggatctac atgctgttta ctggccacct ctttcacgaa cttgtgcaac ggtttctctg
     2341 ctgcagcgct tcttatctga agggccggcg ccttggcgag acgagcgcct ccaagaagag
     2401 caattcttct agctttgtgc tcagtcacag gtcctcttcc cagcgcagtt gttctcagcc
     2461 aagcacagca gccggtggac gcaccccacc cagcctgggt ccccaagatg agtcctgcac
     2521 caccgccagc tcctccctgg ccaaggacac ttcatcgggt accggtgctt tgcacttgga
     2581 tcaaacgcca tctcgccagc ctattcccag tgagggtctt cagctgcatt taccgcaggt
     2641 tttagctgac gctgtctcac gcctggtcct gggtaagttt ggtgacctga ccgacaactt
     2701 ctcctcccct cacgctcgca gaaaagtgct ggctggagtc gtcatgacaa caggcacaga
     2761 tgttaaagat gccaaggtga taagtgtttc tacaggaaca aaatgtatta atggtgaata
     2821 catgagtgat cgtggccttg cattaaatga ctgccatgca gaaataatat ctcggagatc
     2881 cttgctcaga tttctttata cacaacttga gctttactta aataacaaag atgatcaaaa
     2941 aagatccatc tttcagaaat cagagcgagg ggggtttagg ctgaaggaga atgtccagtt
     3001 tcatctgtac atcagcacct ctccctgtgg agatgccaga atcttctcac cacatgagcc
     3061 aatcctggaa gaaccagcag atagacaccc aaatcgtaaa gcaagaggac agctacggac
     3121 caaaatagag tctggtcagg ggacgattcc agtgcgctcc aatgcgagca tccaagcgtg
     3181 ggacggggtg ctgcaagggg agcggctgct caccatgtcc tgcagtgaca agattgcacg
     3241 ctggaacgtg gtgggcatcc agggatccct gctcagcatt ttcgtggagc ccatttactt
     3301 ctcgagcatc atcctgggca gcctttacca cggggaccac ctttccaggg ccatgtacca
     3361 gcggatctcc aacatagagg acctgccacc tctctacacc ctcaacaagc ctttgctcag
     3421 tggcatcagc aatgcagaag cacggcagcc agggaaggcc cccaacttca gtgtcaactg
     3481 gacggtaggc gactccgcta ttgaggtcat caacgccacg actgggaagg atgagctggg
     3541 ccgcgcgtcc cgcctgtgta agcacgcgtt gtactgtcgc tggatgcgtg tgcacggcaa
     3601 ggttccctcc cacttactac gctccaagat taccaaaccc aacgtgtacc atgagtccaa
     3661 gctggcggca aaggagtacc aggccgccaa ggcgcgtctg ttcacagcct tcatcaaggc
     3721 ggggctgggg gcctgggtgg agaagcccac cgagcaggac cagttctcac tcacgccctg
     3781 agccgctcga ggagggcccg aacaaaaact catctcagaa gaggatctga atagcgccgt
     3841 cgaccatcat cataatcatc attgagttta aacccgctga tcagcctcga ctgtgccttc
     3901 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     3961 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     4021 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     4081 tagcaggcat gctggggatg cggtgggctc tatggagatc tatcgatgtt ctggggatgc
     4141 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     4201 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     4261 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     4321 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     4381 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtacctagaa
     4441 gttcctattc cgaagttcct attctctaga aagtatagga acttccttgg ccaaaaagcc
     4501 tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga
     4561 cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg
     4621 tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta
     4681 tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag
     4741 cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc
     4801 tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc
     4861 ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata
     4921 cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac
     4981 tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg
     5041 ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt
     5101 cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga
     5161 ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca
     5221 gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc
     5281 gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga
     5341 tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt
     5401 cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt
     5461 actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaagg aatagcacgt
     5521 actacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     5581 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     5641 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     5701 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     5761 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     5821 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     5881 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     5941 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     6001 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     6061 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     6121 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     6181 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     6241 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     6301 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     6361 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     6421 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     6481 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     6541 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     6601 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     6661 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     6721 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     6781 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     6841 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     6901 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     6961 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     7021 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     7081 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     7141 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     7201 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     7261 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     7321 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     7381 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     7441 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     7501 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     7561 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     7621 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     7681 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactga
//
