LOCUS       sequence-466386-        7642 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  human oxytocin receptor-ADAR2dd(E488Q, T501A), no V2 tail.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7642)
  AUTHORS   Zhang X, Mille-Fragoso LS, Kaseniit KE, Lee AP, Zhang M, Call CC, Hu
            Y, Xie Y, Gao XJ
  TITLE     Post-transcriptional modular synthetic receptors.
  JOURNAL   Nat Chem Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w.
  PUBMED    40155716
REFERENCE   2  (bases 1 to 7642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w."
COMMENT     Sequence Label: XZ113 CMV-TO-signal-hOXTR-ADAR2dd(DD-E488Q-T50...
FEATURES             Location/Qualifiers
     source          1..7642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     41..60
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(279..383)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(458..477)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        644..1023
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1024..1227
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1178..1198
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    1229..1247
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1250..1268
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1278..1302
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3884..3913
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(3969..3986)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3975..4199
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4371..4390)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4522..4569
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4577..5599
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4577..5599
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    5729..5850
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5766..5785)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5820..5839
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5899..5915)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5899..5915)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5912..5934)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5923..5939
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5947..5953,5954..5971,5972..5977))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5992..6013
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6130..6147)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6301..6889)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6381..6400)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7060..7642,1..209,210..278))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
       61 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
      121 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
      181 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
      241 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
      301 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
      361 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
      421 tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat
      481 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
      541 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg
      601 ttaggcgttt tgcgctgctt cgtacgggcc agatatacgc gttgacattg attattgact
      661 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      721 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      781 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      841 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      901 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      961 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     1021 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     1081 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     1141 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     1201 cggtgggagg tctatataag cagagctctc cctatcagtg atagagatct ccctatcagt
     1261 gatagagatc gtcgacgagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca
     1321 cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccggac tctagcgttt
     1381 aaacttaagc ttcgccacca tgaagacgat catcgccctg agctacatct tctgcctggt
     1441 attcgccgac tacaaggacg atgatgacgc cagcggcgaa ggggcccttg ccgccaattg
     1501 gtctgccgag gcggcgaacg ctagtgcggc cccccctggc gcggaaggta accgaacggc
     1561 aggcccacct aggagaaatg aagctttggc gagagtggag gtggccgtcc tctgccttat
     1621 tctgctgttg gccttgtccg gaaacgcttg tgtgctgctc gcgctccgca cgacccggca
     1681 gaagcactcc agactgtttt tttttatgaa gcaccttagc atcgctgacc tcgtcgttgc
     1741 ggtattccag gtgctgcccc aactgctgtg ggatatcaca ttcaggtttt acggcccaga
     1801 cttgctctgt agacttgtca agtacctcca ggttgtcgga atgttcgcgt ccacatacct
     1861 ccttcttttg atgtcactgg accgatgtct ggcaatatgt cagcccctta ggagcctgcg
     1921 gagaagaacg gacaggctcg ctgtactcgc tacatggctc ggctgtcttg tggcatccgc
     1981 acctcaggtt catatctttt cactcagaga agtggcagat ggcgtgtttg actgttgggc
     2041 cgtattcatt cagccatggg gacccaaggc gtatatcacc tggatcacgt tggctgtgta
     2101 tatcgttcct gttatcgtgt tggccgcttg ctacggcctc atctcattca aaatttggca
     2161 gaacctccgc ttgaaaacag ctgccgctgc tgctgcagaa gctccagaag gcgcggcagc
     2221 tggcgacggt ggacgggttg cactcgccag ggtcagtagc gttaagctca tcagcaaggc
     2281 aaagatccgg actgttaaaa tgacctttat tattgtgctt gcttttattg tgtgttggac
     2341 tcccttcttc tttgtacaga tgtggagcgt gtgggacgct aacgccccca aggaggcgag
     2401 cgcgttcatt atagtgatgt tgctcgcgtc cctgaattcc tgttgtaacc cgtggatcta
     2461 catgctgttt actggccacc tctttcacga acttgtgcaa cggtttctct gctgcagcgc
     2521 ttcttatctg aagggccggc gccttggcga gacgagcgcc tccaagaaga gcaattcttc
     2581 tagctttgtg ctcagtcaca ggtcctcttc ccagcgcagt tgttctcagc caagcacagc
     2641 aggtaccggt gctttgcact tggatcaaac gccatctcgc cagcctattc ccagtgaggg
     2701 tcttcagctg catttaccgc aggttttagc tgacgctgtc tcacgcctgg tcctgggtaa
     2761 gtttggtgac ctgaccgaca acttctcctc ccctcacgct cgcagaaaag tgctggctgg
     2821 agtcgtcatg acaacaggca cagatgttaa agatgccaag gtgataagtg tttctacagg
     2881 aacaaaatgt attaatggtg aatacatgag tgatcgtggc cttgcattaa atgactgcca
     2941 tgcagaaata atatctcgga gatccttgct cagatttctt tatacacaac ttgagcttta
     3001 cttaaataac aaagatgatc aaaaaagatc catctttcag aaatcagagc gaggggggtt
     3061 taggctgaag gagaatgtcc agtttcatct gtacatcagc acctctccct gtggagatgc
     3121 cagaatcttc tcaccacatg agccaatcct ggaagaacca gcagatagac acccaaatcg
     3181 taaagcaaga ggacagctac ggaccaaaat agagtctggt caggggacga ttccagtgcg
     3241 ctccaatgcg agcatccaag cgtgggacgg ggtgctgcaa ggggagcggc tgctcaccat
     3301 gtcctgcagt gacaagattg cacgctggaa cgtggtgggc atccagggat ccctgctcag
     3361 cattttcgtg gagcccattt acttctcgag catcatcctg ggcagccttt accacgggga
     3421 ccacctttcc agggccatgt accagcggat ctccaacata gaggacctgc cacctctcta
     3481 caccctcaac aagcctttgc tcagtggcat cagcaatgca gaagcacggc agccagggaa
     3541 ggcccccaac ttcagtgtca actggacggt aggcgactcc gctattgagg tcatcaacgc
     3601 cacgactggg aaggatgagc tgggccgcgc gtcccgcctg tgtaagcacg cgttgtactg
     3661 tcgctggatg cgtgtgcacg gcaaggttcc ctcccactta ctacgctcca agattaccaa
     3721 acccaacgtg taccatgagt ccaagctggc ggcaaaggag taccaggccg ccaaggcgcg
     3781 tctgttcaca gccttcatca aggcggggct gggggcctgg gtggagaagc ccaccgagca
     3841 ggaccagttc tcactcacgc cctgagccgc tcgaggaggg cccgaacaaa aactcatctc
     3901 agaagaggat ctgaatagcg ccgtcgacca tcatcataat catcattgag tttaaacccg
     3961 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     4021 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     4081 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     4141 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatgga
     4201 gatctatcga tgttctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     4261 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     4321 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     4381 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     4441 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     4501 gggtgatggt tcacgtacct agaagttcct attccgaagt tcctattctc tagaaagtat
     4561 aggaacttcc ttggccaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat
     4621 cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc
     4681 tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg
     4741 tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga
     4801 agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca
     4861 gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc
     4921 ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt
     4981 cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga
     5041 tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca
     5101 ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca
     5161 cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga
     5221 ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag
     5281 gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct
     5341 tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca
     5401 gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat
     5461 cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt
     5521 ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg
     5581 tccgagggca aaggaatagc acgtactacg agatttcgat tccaccgccg ccttctatga
     5641 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     5701 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     5761 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     5821 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     5881 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     5941 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     6001 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     6061 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     6121 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     6181 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     6241 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     6301 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     6361 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     6421 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     6481 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     6541 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     6601 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     6661 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     6721 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     6781 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     6841 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     6901 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     6961 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     7021 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     7081 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     7141 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     7201 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     7261 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     7321 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     7381 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     7441 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     7501 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     7561 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     7621 caagtcattc tgagaatagt gt
//
