LOCUS       sequence-466392-        7801 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  _-2 adrenergic receptor-V2-ADAR2dd(E488Q, T501A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7801)
  AUTHORS   Zhang X, Mille-Fragoso LS, Kaseniit KE, Lee AP, Zhang M, Call CC, Hu
            Y, Xie Y, Gao XJ
  TITLE     Post-transcriptional modular synthetic receptors.
  JOURNAL   Nat Chem Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w.
  PUBMED    40155716
REFERENCE   2  (bases 1 to 7801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w."
COMMENT     Sequence Label: XZ110 CMV-TO-signal-B2AR-V2-ADAR2dd(DD-E488Q-T...
FEATURES             Location/Qualifiers
     source          1..7801
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(49..68)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    200..247
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             255..1277
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             255..1277
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    1407..1528
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1444..1463)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1498..1517
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1577..1593)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1577..1593)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1590..1612)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1601..1617
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1625..1631,1632..1649,1650..1655))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1670..1691
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1808..1825)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1979..2567)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2059..2078)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2738..3529,3530..3598))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3361..3380
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3599..3703)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3778..3797)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3964..4343
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4344..4547
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4498..4518
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4549..4567
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4570..4588
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4598..4622
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             7363..7392
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(7448..7465)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7454..7678
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
       61 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
      121 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
      181 gtgatggttc acgtacctag aagttcctat tccgaagttc ctattctcta gaaagtatag
      241 gaacttcctt ggccaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg
      301 aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt
      361 tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt
      421 tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag
      481 tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg
      541 gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg
      601 aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg
      661 gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc
      721 cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg
      781 ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg
      841 cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact
      901 ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc
      961 cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg
     1021 caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga
     1081 gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg
     1141 tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct
     1201 ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc
     1261 cgagggcaaa ggaatagcac gtactacgag atttcgattc caccgccgcc ttctatgaaa
     1321 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     1381 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     1441 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     1501 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     1561 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     1621 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     1681 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     1741 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     1801 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     1861 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     1921 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     1981 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     2041 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     2101 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     2161 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     2221 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     2281 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     2341 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     2401 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     2461 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     2521 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     2581 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     2641 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     2701 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     2761 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     2821 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     2881 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     2941 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     3001 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     3061 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     3121 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     3181 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     3241 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     3301 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     3361 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     3421 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     3481 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     3541 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     3601 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     3661 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     3721 tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac
     3781 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt
     3841 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg
     3901 catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgtacgggcc agatatacgc
     3961 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     4021 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     4081 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     4141 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     4201 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     4261 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     4321 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     4381 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     4441 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     4501 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc cctatcagtg
     4561 atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg aaccgtcaga
     4621 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
     4681 gcctccggac tctagcgttt aaacttaagc ttcgccacca tgaagacgat catcgccctg
     4741 agctacatct tctgcctggt attcgccgac tacaaggacg atgatgacgc cagcggcggg
     4801 caacccggga acggcagcgc cttcttgctg gcacccaata gaagccatgc gccggaccac
     4861 gacgtcacgc agcaaaggga cgaggtgtgg gtggtgggca tgggcatcgt catgtctctc
     4921 atcgtcctgg ccatcgtgtt tggcaatgtg ctggtcatca cagccattgc caagttcgag
     4981 cgtctgcaga cggtcaccaa ctacttcatc acttcactgg cctgtgctga tctggtcatg
     5041 ggcctggcag tggtgccctt tggggccgcc catattctta tgaaaatgtg gacttttggc
     5101 aacttctggt gcgagttttg gacttccatt gatgtgctgt gcgtcacggc cagcattgag
     5161 accctgtgcg tgatcgcagt ggatcgctac tttgccatta cttcaccttt caagtaccag
     5221 agcctgctga ccaagaataa ggcccgggtg atcattctga tggtgtggat tgtgtcaggc
     5281 cttacctcct tcttgcccat tcagatgcac tggtatcggg ccacccacca ggaagccatc
     5341 aactgctatg ccaatgagac ctgctgtgac ttcttcacga accaagccta tgccattgcc
     5401 tcttccatcg tgtccttcta cgttcccctg gtgatcatgg tcttcgtcta ctccagggtc
     5461 tttcaggagg ccaaaaggca gctccagaag attgacaaat ctgagggccg cttccatgtc
     5521 cagaacctta gccaggtgga gcaggatggg cggacggggc atggactccg cagatcttcc
     5581 aagttctgct tgaaggagca caaagccctc aagacgttag gcatcatcat gggcactttc
     5641 accctctgct ggctgccctt cttcatcgtt aacattgtgc atgtgatcca ggataacctc
     5701 atccgtaagg aagtttacat cctcctaaat tggataggct atgtcaattc tggtttcaat
     5761 ccccttatct actgccggag cccagatttc aggattgcct tccaggagct tctgtgcctg
     5821 cgcaggtctt ctttgaaggc ctatgggaat ggctactcca gcaacggcaa cacaggggag
     5881 cagagtggat atcacgtgga acaggagaaa gaaaataaac tgctgtgtga agacctccca
     5941 ggcacggaag actttgtggg ccatcaaggt actgtgccta gcgataacat tgattcacaa
     6001 gggaggaatt gtagtacaaa tgactcactg ctggccggtg gacgcacccc acccagcctg
     6061 ggtccccaag atgagtcctg caccaccgcc agctcctccc tggccaagga cacttcatcg
     6121 ggtaccggtg ctttgcactt ggatcaaacg ccatctcgcc agcctattcc cagtgagggt
     6181 cttcagctgc atttaccgca ggttttagct gacgctgtct cacgcctggt cctgggtaag
     6241 tttggtgacc tgaccgacaa cttctcctcc cctcacgctc gcagaaaagt gctggctgga
     6301 gtcgtcatga caacaggcac agatgttaaa gatgccaagg tgataagtgt ttctacagga
     6361 acaaaatgta ttaatggtga atacatgagt gatcgtggcc ttgcattaaa tgactgccat
     6421 gcagaaataa tatctcggag atccttgctc agatttcttt atacacaact tgagctttac
     6481 ttaaataaca aagatgatca aaaaagatcc atctttcaga aatcagagcg aggggggttt
     6541 aggctgaagg agaatgtcca gtttcatctg tacatcagca cctctccctg tggagatgcc
     6601 agaatcttct caccacatga gccaatcctg gaagaaccag cagatagaca cccaaatcgt
     6661 aaagcaagag gacagctacg gaccaaaata gagtctggtc aggggacgat tccagtgcgc
     6721 tccaatgcga gcatccaagc gtgggacggg gtgctgcaag gggagcggct gctcaccatg
     6781 tcctgcagtg acaagattgc acgctggaac gtggtgggca tccagggatc cctgctcagc
     6841 attttcgtgg agcccattta cttctcgagc atcatcctgg gcagccttta ccacggggac
     6901 cacctttcca gggccatgta ccagcggatc tccaacatag aggacctgcc acctctctac
     6961 accctcaaca agcctttgct cagtggcatc agcaatgcag aagcacggca gccagggaag
     7021 gcccccaact tcagtgtcaa ctggacggta ggcgactccg ctattgaggt catcaacgcc
     7081 acgactggga aggatgagct gggccgcgcg tcccgcctgt gtaagcacgc gttgtactgt
     7141 cgctggatgc gtgtgcacgg caaggttccc tcccacttac tacgctccaa gattaccaaa
     7201 cccaacgtgt accatgagtc caagctggcg gcaaaggagt accaggccgc caaggcgcgt
     7261 ctgttcacag ccttcatcaa ggcggggctg ggggcctggg tggagaagcc caccgagcag
     7321 gaccagttct cactcacgcc ctgagccgct cgaggagggc ccgaacaaaa actcatctca
     7381 gaagaggatc tgaatagcgc cgtcgaccat catcataatc atcattgagt ttaaacccgc
     7441 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     7501 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     7561 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     7621 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggag
     7681 atctatcgat gttctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     7741 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     7801 g
//
