LOCUS       sequence-466394-        7745 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  IVT template for LIDAR reporter mRNA; EGFP as marker, Cre 
            recombinase as output.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7745)
  AUTHORS   Zhang X, Mille-Fragoso LS, Kaseniit KE, Lee AP, Zhang M, Call CC, Hu
            Y, Xie Y, Gao XJ
  TITLE     Post-transcriptional modular synthetic receptors.
  JOURNAL   Nat Chem Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w.
  PUBMED    40155716
REFERENCE   2  (bases 1 to 7745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w."
COMMENT     Sequence Label: XZ388 (IVT)
            CMV-TO-T7-EGFP-SGc(stem-1(3xMS2))c...
FEATURES             Location/Qualifiers
     source          1..7745
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     24..43
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    215..439
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     428..445
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(501..530)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(832..1863)
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MANLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             complement(1870..1935)
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             complement(2104..2169)
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             complement(join(2212..2922,2923..2925,2926..2928))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(2212..2922,2923..2925,2926..2928))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2254..2275)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2603..2622
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     regulatory      2925..2934
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3086..3105)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3087..3105)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3215..3239)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3249..3267
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3270..3288
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(3290..3493)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3319..3339)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3494..3873
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     4040..4059
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4134..4238
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4239..4307,4308..5099)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4457..4476)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5270..5858
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5759..5778
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6012..6029
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6146..6167
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6182..6187,6188..6205,6206..6212)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6220..6236
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6225..6247
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6244..6260
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6244..6260
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    6309..6430
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6320..6339)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6374..6393
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6560..7582)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             complement(6560..7582)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     protein_bind    complement(7590..7637)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
       61 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
      121 cagggcgcgt ggggataccc cctagagccc cagctggttc tttccgcctc agaagccata
      181 gagcccaccg catccccaga acatcgatag atctccatag agcccaccgc atccccagca
      241 tgcctgctat tgtcttccca atcctccccc ttgctgtcct gccccacccc accccccaga
      301 atagaatgac acctactcag acaatgcgat gcaatttcct cattttatta ggaaaggaca
      361 gtgggagtgg caccttccag ggtcaaggaa ggcacggggg aggggcaaac aacagatggc
      421 tggcaactag aaggcacagt cgaggctgat cagcgggttt aaactcaatg atgattatga
      481 tgatggtcga cggcgctatt cagatcctct tctgagatga gtttttgttc gggcccgcta
      541 gctccagggt gtggctggca cgaaattgac caaccctggg gttagtatag cttagttaaa
      601 ctttcgttta ttgctaaagg ttaatcactg ctgtttcccg tgggggtgtg gctaggctaa
      661 gcgttttgag ctgcattgct gcgtgcttgg gaggtgtctg gaactagcag aggtggtgag
      721 tggggcaggt ggaggtggga gcatacctgg gacccgaggt cgggggagac tcggggtacc
      781 caggacggga aaggggcagc tagcattgcg tgcatgcagt accagctcga gtcaatcgcc
      841 atcttccagc aggcgcacca ttgcccctgt ttcactatcc aggttacgga tatagttcat
      901 gacaatattt acattggtcc agccaccagc ttgcatgatc tccggtattg aaactccagc
      961 gcgggccata tctcgcgcgg ctccgacacg ggcactgtgt ccagaccagg ccaggtatct
     1021 ctgaccagag tcatccttag cgccgtaaat caatcgatga gttgcttcaa aaatcccttc
     1081 cagggcgcga gttgatagct ggctggtggc agatggcgcg gcaacaccat tttttctgac
     1141 ccggcaaaac aggtagttat tcggatcatc agctacacca gagacggaaa tccatcgctc
     1201 gaccagttta gttaccccca ggctaagtgc cttctctaca cctgcggtgc taaccagcgt
     1261 tttcgttctg ccaatatgga ttaacattct cccaccgtca gtacgtgaga tatctttaac
     1321 cctgatcctg gcaatttcgg ctatacgtaa cagggtgtta taagcaatcc ccagaaatgc
     1381 cagattacgt atatcctggc agcgatcgct attttccatg agtgaacgaa cctggtcgaa
     1441 atcagtgcgt tcgaacgcta gagcctgttt tgcacgttca ccggcatcaa cgttttcttt
     1501 tctgatccgc cgcataacca gtgaaacagc attgctgtca cttggtcgtg gcagcccgga
     1561 ccgacgatga agcatgttta gctggcccaa atgttgctgg atagttttta ctgccagacc
     1621 gcgcgcctga agatatagaa gataatcgcg aacatcttca ggttctgcgg gaaaccattt
     1681 ccggttattc aacttgcacc atgccgccca cgaccggcaa acggacagaa gcattttcca
     1741 ggtatgctca gaaaacgcct ggcgatccct gaacatgtcc atcaggttct tgcgaacctc
     1801 atcactcgtt gcatcgaccg gcaatgcagg caaattttgg tgtacggtca gtaaattggc
     1861 catactcgag ggtcctggat tagattccac atcacctgcg agtttgagta gatcgaagtt
     1921 gagtgtttgc tttactgggg ctacgatctt ttgcttatgc cgtgcctcac tagtaccggt
     1981 cgtctgggta ggtgggatac tatggtacgc tgatgctgta ccagacagac cctgatggtg
     2041 tctgagacgt accctgatgg tgtacgttac tattccaccc acctaaacgg aggatccggt
     2101 accgggaccg gggttggact cgacgtctcc cgccagcttc agaaggtcaa aattcaaagt
     2161 ctgtttcacc ggagccacaa ttttctgttt gtgtctggct tccaacgcgt ccttatacag
     2221 ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc
     2281 gcgcttctcg ttggggtctt tgctcagggc ggactgggtg ctcaggtagt ggttgtcggg
     2341 cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac
     2401 gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt tcttctgctt
     2461 gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt gccccaggat
     2521 gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc
     2581 ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg
     2641 ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc
     2701 ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg
     2761 cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc
     2821 gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac
     2881 caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatgg tggcgaagct
     2941 ttatcgttcg taaatggtga aaattttcag aaaattgctt ttgctttaaa agaaatgatt
     3001 taaattgctg caatagaagt agaatgcttg attgcttgag attcgtttgt tttgtatatg
     3061 ttgtgttgag atttgttatt tttctcccta tagtgagtcg tattagcggc cgcggatctc
     3121 tagcgagctt aagtttaaac gctagagtcc ggaggctgga tcggtcccgg tgtcttctat
     3181 ggaggtcaaa acagcgtgga tggcgtctcc aggcgatctg acggttcact aaacgagctc
     3241 gtcgacgatc tctatcactg atagggagat ctctatcact gatagggaga gctctgctta
     3301 tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac
     3361 gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg
     3421 ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc
     3481 aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa
     3541 agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt
     3601 caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc
     3661 gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat
     3721 acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta
     3781 ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg
     3841 attactatta ataactagtc aataatcaat gtcaacgcgt atatctggcc cgtacgaagc
     3901 agcgcaaaac gcctaaccct aagcagattc ttcatgcaat tgtcggtcaa gccttgcctt
     3961 gttgtagctt aaattttgct cgcgcactac tcagcgacct ccaacacaca agcagggagc
     4021 agatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatagggga
     4081 tcgggagatc tcccgatccg tcgacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     4141 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     4201 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     4261 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     4321 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     4381 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     4441 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     4501 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     4561 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     4621 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     4681 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     4741 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     4801 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     4861 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     4921 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     4981 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     5041 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     5101 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     5161 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     5221 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     5281 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     5341 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     5401 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     5461 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     5521 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     5581 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     5641 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     5701 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     5761 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     5821 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     5881 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     5941 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     6001 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     6061 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     6121 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     6181 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     6241 acacaggaaa cagctatgac catgattacg ccaagctcta gctagaggtc gacggtatac
     6301 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     6361 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     6421 taaacaagtt ggggtgggcg aagaactcca gcatgagatc cccgcgctgg aggatcatcc
     6481 agccggcgtc ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat
     6541 cgaaatctcg tagtacgtgc tattcctttg ccctcggacg agtgctgggg cgtcggtttc
     6601 cactatcggc gagtacttct acacagccat cggtccagac ggccgcgctt ctgcgggcga
     6661 tttgtgtacg cccgacagtc ccggctccgg atcggacgat tgcgtcgcat cgaccctgcg
     6721 cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg tcaagaccaa
     6781 tgcggagcat atacgcccgg agccgcggcg atcctgcaag ctccggatgc ctccgctcga
     6841 agtagcgcgt ctgctgctcc atacaagcca accacggcct ccagaagaag atgttggcga
     6901 cctcgtattg ggaatccccg aacatcgcct cgctccagtc aatgaccgct gttatgcggc
     6961 cattgtccgt caggacattg ttggagccga aatccgcgtg cacgaggtgc cggacttcgg
     7021 ggcagtcctc ggcccaaagc atcagctcat cgagagcctg cgcgacggac gcactgacgg
     7081 tgtcgtccat cacagtttgc cagtgataca catggggatc agcaatcgcg catatgaaat
     7141 cacgccatgt agtgtattga ccgattcctt gcggtccgaa tgggccgaac ccgctcgtct
     7201 ggctaagatc ggccgcagcg atcgcatcca tggcctccgc gaccggctgc agaacagcgg
     7261 gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg tgcacggcgg gagatgcaat
     7321 aggtcaggct ctcgctgaat tccccaatgt caagcacttc cggaatcggg agcgcggccg
     7381 atgcaaagtg ccgataaaca taacgatctt tgtagaaacc atcggcgcag ctatttaccc
     7441 gcaggacata tccacgccct cctacatcga agctgaaagc acgagattct tcgccctccg
     7501 agagctgcat caggtcggag acgctgtcga acttttcgat cagaaacttc tcgacagacg
     7561 tcgcggtgag ttcaggcttt ttggccaagg aagttcctat actttctaga gaataggaac
     7621 ttcggaatag gaacttctag gtacgtgaac catcacccta atcaagtttt ttggggtcga
     7681 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     7741 gaaag
//
