LOCUS       sequence-466395-        7817 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  IVT template for LIDAR reporter mRNA; mCherry as marker, rtetR-NZF 
            as output.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7817)
  AUTHORS   Zhang X, Mille-Fragoso LS, Kaseniit KE, Lee AP, Zhang M, Call CC, Hu
            Y, Xie Y, Gao XJ
  TITLE     Post-transcriptional modular synthetic receptors.
  JOURNAL   Nat Chem Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w.
  PUBMED    40155716
REFERENCE   2  (bases 1 to 7817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w."
COMMENT     Sequence Label: XZ390 (IVT)
            CMV-TO-T7-mCherry-SGc(stem-1(3xMS2...
FEATURES             Location/Qualifiers
     source          1..7817
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(993..1040)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     1172..1191
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    1363..1587
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     1576..1593
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(1649..1678)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(1983..2003)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(3096..3161)
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             complement(3330..3395)
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             complement(3441..4148)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3716..3735)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3985..4003
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      4145..4154
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4306..4325)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4307..4325)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4435..4459)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    4469..4487
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4490..4508
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(4510..4713)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4539..4559)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4714..5093
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     5260..5279
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5354..5458
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5459..5527,5528..6319)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5677..5696)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6490..7078
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6979..6998
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7232..7249
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7366..7387
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7402..7407,7408..7425,7426..7432)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7440..7456
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7445..7467
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7464..7480
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7464..7480
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    7529..7650
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7540..7559)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7594..7613
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(7780..7817,1..985))
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             complement(join(7780..7817,1..985))
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
ORIGIN
        1 ttccactatc ggcgagtact tctacacagc catcggtcca gacggccgcg cttctgcggg
       61 cgatttgtgt acgcccgaca gtcccggctc cggatcggac gattgcgtcg catcgaccct
      121 gcgcccaagc tgcatcatcg aaattgccgt caaccaagct ctgatagagt tggtcaagac
      181 caatgcggag catatacgcc cggagccgcg gcgatcctgc aagctccgga tgcctccgct
      241 cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg cctccagaag aagatgttgg
      301 cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc
      361 ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg tgccggactt
      421 cggggcagtc ctcggcccaa agcatcagct catcgagagc ctgcgcgacg gacgcactga
      481 cggtgtcgtc catcacagtt tgccagtgat acacatgggg atcagcaatc gcgcatatga
      541 aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg aacccgctcg
      601 tctggctaag atcggccgca gcgatcgcat ccatggcctc cgcgaccggc tgcagaacag
      661 cgggcagttc ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc
      721 aataggtcag gctctcgctg aattccccaa tgtcaagcac ttccggaatc gggagcgcgg
      781 ccgatgcaaa gtgccgataa acataacgat ctttgtagaa accatcggcg cagctattta
      841 cccgcaggac atatccacgc cctcctacat cgaagctgaa agcacgagat tcttcgccct
      901 ccgagagctg catcaggtcg gagacgctgt cgaacttttc gatcagaaac ttctcgacag
      961 acgtcgcggt gagttcaggc tttttggcca aggaagttcc tatactttct agagaatagg
     1021 aacttcggaa taggaacttc taggtacgtg aaccatcacc ctaatcaagt tttttggggt
     1081 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     1141 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     1201 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     1261 cgccgctaca gggcgcgtgg ggataccccc tagagcccca gctggttctt tccgcctcag
     1321 aagccataga gcccaccgca tccccagaac atcgatagat ctccatagag cccaccgcat
     1381 ccccagcatg cctgctattg tcttcccaat cctccccctt gctgtcctgc cccaccccac
     1441 cccccagaat agaatgacac ctactcagac aatgcgatgc aatttcctca ttttattagg
     1501 aaaggacagt gggagtggca ccttccaggg tcaaggaagg cacgggggag gggcaaacaa
     1561 cagatggctg gcaactagaa ggcacagtcg aggctgatca gcgggtttaa actcaatgat
     1621 gattatgatg atggtcgacg gcgctattca gatcctcttc tgagatgagt ttttgttcgg
     1681 gcccgctagc tccagggtgt ggctggcacg aaattgacca accctggggt tagtatagct
     1741 tagttaaact ttcgtttatt gctaaaggtt aatcactgct gtttcccgtg ggggtgtggc
     1801 taggctaagc gttttgagct gcattgctgc gtgcttggga ggtgtctgga actagcagag
     1861 gtggtgagtg gggcaggtgg aggtgggagc atacctggga cccgaggtcg ggggagactc
     1921 ggggtaccca ggacgggaaa ggggcagcta gcattgcgtg catgcagtac cagctcgagc
     1981 tacaccttgc gttttttctt gggagatccg ctgtcgaagt tgaagtccag gccgtcggcg
     2041 tccatcagct cgcttctgat gatgctctcc atgtcgcact ccaggctgcc gttgaacatg
     2101 tccaggtcca ggtcgctggg gaacttctcg tgtcccaaac caaggccgtc tcctccgccg
     2161 ggggggtcca gcaggaacag gtcctggcag ttgtccaggg cggtgtccca gaaggtgtcg
     2221 ccctgctcca ggcccagctg ctcccagtcg cccagggtga agtccatgcc cacgtccttc
     2281 agggtcacga attcctcggc catagatccc cagcctccgt cctgcttggg ctccagggcc
     2341 tggccctggt tcaccagctc ggggatgccc agggctctgt cgatctcctc caggccggtg
     2401 gcgtcggtgt tgctcagcag ggtgtgcagc tggtccagca gggctctctc gtcgctctgg
     2461 ccctcggtcg gcccgccgct ttcgcacttt agctgtttct ccaggccaca aatgattagt
     2521 tccaggccga aaaggaaggc aggttcggct ccctgccggt cgaacagctc aattgcttgt
     2581 ctcagaagtg ggggcataga atcggtggta ggtgtctctc tttcctcttt tgctacttga
     2641 tgctcctgtt cctccaatac gcagcccagt gtaaagtggc ccacggcgga cagagcgtac
     2701 agtgcgttct ccagggagaa gccttgctga cacaggaacg cgagctgatt ttccagggtt
     2761 tcgtactgtt tctctgttgg gcgggtgccg agatgcactt tagccccgtc gcgatgtgag
     2821 aggagagcac agcggtatga cttggcgttg ttccgcagaa agtcttgcca tgactcgcct
     2881 tccagggggc agaagtgggt atgatgcctg tccagcatct cgattggcag ggcatcgagc
     2941 agagcccgct tgttcttcac gtgccagtac agggtaggct gctcaactcc cagcttttga
     3001 gcgagtttcc ttgtcgtcag gccttcgata ccgacaccat tgagtaattc cagagctccg
     3061 tttatgactt tgctcttgtc cagcctagaa ctcgagggtc ctggattaga ttccacatca
     3121 cctgcgagtt tgagtagatc gaagttgagt gtttgcttta ctggggctac gatcttttgc
     3181 ttatgccgtg cctcactagt accggtcgtc tgggtaggtg ggatactatg gtacgctgat
     3241 gctgtaccag acagaccctg atggtgtctg agacgtaccc tgatggtgta cgttactatt
     3301 ccacccacct aaacggagga tccggtaccg ggaccggggt tggactcgac gtctcccgcc
     3361 agcttcagaa ggtcaaaatt caaagtctgt ttcaccggag ccacaatttt ctgtttgtgt
     3421 ctggcttcca acgcgtcgct cttgtacagc tcgtccatgc cgccggtgga gtggcggccc
     3481 tcggcgcgtt cgtactgttc cacgatggtg tagtcctcgt tgtgggaggt gatgtccaac
     3541 ttgatgttga cgttgtaggc gccgggcagc tgcacgggct tcttggcctt gtaggtggtc
     3601 ttgacctcag cgtcgtagtg gccgccgtcc ttcagcttca gcctctgctt gatctcgccc
     3661 ttcagggcgc cgtcctcggg gtacatccgc tcggaggagg cctcccagcc catggtcttc
     3721 ttctgcatta cggggccgtc ggaggggaag ttggtgccgc gcagcttcac cttgtagatg
     3781 aactcgccgt cctgcaggga ggagtcctgg gtcacggtca ccacgccgcc gtcctcgaag
     3841 ttcatcacgc gctcccactt gaagccctcg gggaaggaca gcttcaagta gtcgggaatg
     3901 tcggcggggt gcttcacgta ggccttggag ccgtacatga actgagggga caggatgtcc
     3961 caggcgaagg gcagggggcc acccttggtc accttcagct tggcggtctg ggtgccctcg
     4021 taggggcggc cctcgccctc gccctcgatc tcgaactcgt ggccgttcac ggagccctcc
     4081 atgtgcacct tgaagcgcat gaactccttg atgatggcca tgttatcctc ctcgcccttg
     4141 ctcaccatgg tggcgaagct ttatcgttcg taaatggtga aaattttcag aaaattgctt
     4201 ttgctttaaa agaaatgatt taaattgctg caatagaagt agaatgcttg attgcttgag
     4261 attcgtttgt tttgtatatg ttgtgttgag atttgttatt tttctcccta tagtgagtcg
     4321 tattagcggc cgcggatctc tagcgagctt aagtttaaac gctagagtcc ggaggctgga
     4381 tcggtcccgg tgtcttctat ggaggtcaaa acagcgtgga tggcgtctcc aggcgatctg
     4441 acggttcact aaacgagctc gtcgacgatc tctatcactg atagggagat ctctatcact
     4501 gatagggaga gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt
     4561 caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa
     4621 actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc
     4681 acgcccattg atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta
     4741 gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg
     4801 ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac
     4861 tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat
     4921 tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg
     4981 tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg
     5041 aactaatgac cccgtaattg attactatta ataactagtc aataatcaat gtcaacgcgt
     5101 atatctggcc cgtacgaagc agcgcaaaac gcctaaccct aagcagattc ttcatgcaat
     5161 tgtcggtcaa gccttgcctt gttgtagctt aaattttgct cgcgcactac tcagcgacct
     5221 ccaacacaca agcagggagc agatactggc ttaactatgc ggcatcagag cagattgtac
     5281 tgagagtgca ccatagggga tcgggagatc tcccgatccg tcgacgtcag gtggcacttt
     5341 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     5401 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     5461 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     5521 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     5581 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     5641 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     5701 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     5761 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     5821 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     5881 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     5941 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     6001 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     6061 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     6121 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     6181 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     6241 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     6301 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     6361 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     6421 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6481 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6541 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6601 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     6661 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6721 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     6781 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     6841 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     6901 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     6961 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     7021 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     7081 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     7141 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     7201 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     7261 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     7321 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     7381 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     7441 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcta
     7501 gctagaggtc gacggtatac agacatgata agatacattg atgagtttgg acaaaccaca
     7561 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     7621 gtaaccatta taagctgcaa taaacaagtt ggggtgggcg aagaactcca gcatgagatc
     7681 cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc ccaacctttc
     7741 atagaaggcg gcggtggaat cgaaatctcg tagtacgtgc tattcctttg ccctcggacg
     7801 agtgctgggg cgtcggt
//
