LOCUS       sequence-466398-        7669 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Shc1-tdMCP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7669)
  AUTHORS   Zhang X, Mille-Fragoso LS, Kaseniit KE, Lee AP, Zhang M, Call CC, Hu
            Y, Xie Y, Gao XJ
  TITLE     Post-transcriptional modular synthetic receptors.
  JOURNAL   Nat Chem Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w.
  PUBMED    40155716
REFERENCE   2  (bases 1 to 7669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w."
COMMENT     Sequence Label: XZ039 CMV-TO-Shc1-MCP (FLP-IN)
FEATURES             Location/Qualifiers
     source          1..7669
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      672..681
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(773..797)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    807..825
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    828..846
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(848..1051)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(877..897)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1052..1431
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     1598..1617
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1692..1796
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1797..1865,1866..2657)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2015..2034)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2828..3416
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3317..3336
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3570..3587
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3704..3725
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3740..3745,3746..3763,3764..3770)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3778..3794
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3783..3805
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3802..3818
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3802..3818
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    3867..3988
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3878..3897)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3932..3951
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(4118..5140)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             complement(4118..5140)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     protein_bind    complement(5148..5195)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     5327..5346
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    5518..5742
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     5731..5748
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(5804..5833)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 caggttactc ctccccagga tagagctgag cgggcggcta cagggctttc tcctccttgt
       61 cgccccctta gcacccggca cagcctcaca caccagactg atggcctccc tggtgacctg
      121 agtccgggtg ttgaagtcca gggcacgcat tgactggagg acctccacac aacccatgta
      181 ccgaaccaag taggaaaccc cgggtcccat gactttgtcg ttgggatgca gccagccccg
      241 cgtgggctta ttgacaaagc tcccgtggcg ggtccactcc tcgcccccaa gctggccccc
      301 ttccacccga gtcctgcgcc cgccgcctcc actcagcttg ttcatgtcct ggaggagggg
      361 tagggggcct gagtctggca tggctgcccc tacgatcccc tccccatcat cagctgccct
      421 tcctggctcc cccttagacc ctgggcgccc cccagccggg ttggccagcc tcaggttgct
      481 catccggggg aagaaggagc acagggtagt gggactatcg tccccaggca gaggaggcag
      541 gatgggcccc agggatgaag ctgatgggga aggcagctcc tccggggggg tggacccaga
      601 agccccttcc tccagcgatg acagagactc attccggagt ggattgtact tgggcttggg
      661 gggcaggaga tccatggtgg cgaagcttaa gtttaaacgc tagagtccgg aggctggatc
      721 ggtcccggtg tcttctatgg aggtcaaaac agcgtggatg gcgtctccag gcgatctgac
      781 ggttcactaa acgagctcgt cgacgatctc tatcactgat agggagatct ctatcactga
      841 tagggagagc tctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca
      901 atggggcgga gttgttacga cattttggaa agtcccgttg attttggtgc caaaacaaac
      961 tcccattgac gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgctatccac
     1021 gcccattgat gtactgccaa aaccgcatca ccatggtaat agcgatgact aatacgtaga
     1081 tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc
     1141 atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg
     1201 ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg
     1261 gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc
     1321 agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat gggctatgaa
     1381 ctaatgaccc cgtaattgat tactattaat aactagtcaa taatcaatgt caacgcgtat
     1441 atctggcccg tacgaagcag cgcaaaacgc ctaaccctaa gcagattctt catgcaattg
     1501 tcggtcaagc cttgccttgt tgtagcttaa attttgctcg cgcactactc agcgacctcc
     1561 aacacacaag cagggagcag atactggctt aactatgcgg catcagagca gattgtactg
     1621 agagtgcacc ataggggatc gggagatctc ccgatccgtc gacgtcaggt ggcacttttc
     1681 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     1741 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     1801 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     1861 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     1921 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     1981 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     2041 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     2101 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     2161 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     2221 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     2281 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     2341 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     2401 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     2461 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     2521 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     2581 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     2641 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     2701 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     2761 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     2821 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     2881 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     2941 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     3001 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     3061 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     3121 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     3181 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     3241 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3301 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     3361 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     3421 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     3481 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     3541 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     3601 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     3661 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     3721 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     3781 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagctctagc
     3841 tagaggtcga cggtatacag acatgataag atacattgat gagtttggac aaaccacaac
     3901 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     3961 aaccattata agctgcaata aacaagttgg ggtgggcgaa gaactccagc atgagatccc
     4021 cgcgctggag gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat
     4081 agaaggcggc ggtggaatcg aaatctcgta gtacgtgcta ttcctttgcc ctcggacgag
     4141 tgctggggcg tcggtttcca ctatcggcga gtacttctac acagccatcg gtccagacgg
     4201 ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat cggacgattg
     4261 cgtcgcatcg accctgcgcc caagctgcat catcgaaatt gccgtcaacc aagctctgat
     4321 agagttggtc aagaccaatg cggagcatat acgcccggag ccgcggcgat cctgcaagct
     4381 ccggatgcct ccgctcgaag tagcgcgtct gctgctccat acaagccaac cacggcctcc
     4441 agaagaagat gttggcgacc tcgtattggg aatccccgaa catcgcctcg ctccagtcaa
     4501 tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca
     4561 cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat cagctcatcg agagcctgcg
     4621 cgacggacgc actgacggtg tcgtccatca cagtttgcca gtgatacaca tggggatcag
     4681 caatcgcgca tatgaaatca cgccatgtag tgtattgacc gattccttgc ggtccgaatg
     4741 ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat cgcatccatg gcctccgcga
     4801 ccggctgcag aacagcgggc agttcggttt caggcaggtc ttgcaacgtg acaccctgtg
     4861 cacggcggga gatgcaatag gtcaggctct cgctgaattc cccaatgtca agcacttccg
     4921 gaatcgggag cgcggccgat gcaaagtgcc gataaacata acgatctttg tagaaaccat
     4981 cggcgcagct atttacccgc aggacatatc cacgccctcc tacatcgaag ctgaaagcac
     5041 gagattcttc gccctccgag agctgcatca ggtcggagac gctgtcgaac ttttcgatca
     5101 gaaacttctc gacagacgtc gcggtgagtt caggcttttt ggccaaggaa gttcctatac
     5161 tttctagaga ataggaactt cggaatagga acttctaggt acgtgaacca tcaccctaat
     5221 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     5281 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     5341 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     5401 ccgccgcgct taatgcgccg ctacagggcg cgtggggata ccccctagag ccccagctgg
     5461 ttctttccgc ctcagaagcc atagagccca ccgcatcccc agaacatcga tagatctcca
     5521 tagagcccac cgcatcccca gcatgcctgc tattgtcttc ccaatcctcc cccttgctgt
     5581 cctgccccac cccacccccc agaatagaat gacacctact cagacaatgc gatgcaattt
     5641 cctcatttta ttaggaaagg acagtgggag tggcaccttc cagggtcaag gaaggcacgg
     5701 gggaggggca aacaacagat ggctggcaac tagaaggcac agtcgaggct gatcagcggg
     5761 tttaaactca atgatgatta tgatgatggt cgacggcgct attcagatcc tcttctgaga
     5821 tgagtttttg ttcgggccct cctcgagcgg ctcagtaaat tccgctattc gcagcgattg
     5881 cggaaggtat cgggttgcca tctttcagaa gtccttgcat ggcttttaca ataagctcgc
     5941 agtcgctatt ggtcgcaaat atcggaatcg tcaattccat attcaagtat gagcgccatg
     6001 cacctttggg gacctccact ttaatggtat acttccggtt ctgggcacta gattggcgta
     6061 cactacaagt caccttatag gcttgtgatc ttgaattgga agagatccac tcggctatcc
     6121 cattcgcaaa gttacttgga gctactgtga cgtctccagt gccgccgttg tccacgagca
     6181 caaactgtgt aaaattggag gccatggacc cgccaccggt tccgccacca tatattcctg
     6241 agttggcggc gatcgcagag ggaatcgggt taccgtcttt caaaagacct tgcatggcct
     6301 tgacaatcag ctcgcagtct gaattagtcg caaagattgg gatcgtaagc tccatattaa
     6361 gataagatcg ccacgcccct tttggcactt ctacctttat ggtatatttt cggttctgtg
     6421 cggaactttg gcgaactgag catgtgactt tatatgcctg actccggctg ttactactta
     6481 tccattctgc aatcccgttc gcaaaattgc tcggcgcaac agttacgtct ccggtgccac
     6541 cattgtcaac caacacaaat tgcgtaaagt tgctcgccat agcgctacca ccgcccagtt
     6601 tccgctccac aggttgctgt agacacagtt cgctgcccgc agagatgatg ggcaagtgat
     6661 tgtccatgtg gtagctgata aggtgactga cactttcaaa gcggtgatcc ttagtccgaa
     6721 ccacaccctc agggtccacc agtagcaaat gcttaggctg cccactctgc aagccagtga
     6781 gcacatactg gccaggtgtg gtcgtgctct ctcgtaccag gaagtcccca ttgagctgca
     6841 gcagtgcctc agcctcccgc cggctcagct tcccatggaa ccagggctcc cctcggagct
     6901 gctcagccat ggacaccgac tggggaggtg gaggcacgcg aagagcatct tcgaagggct
     6961 tcatgtcaaa caggtcccgg ggtgcactgc cattgatagc aggattgggg ggcccagcac
     7021 cacccactgc ttgccgggcc ttgtctaggt tctggacgtt gacataggag ggatcatcaa
     7081 aaagctctct gcctggacag ggtggtggag gtggcatctg tttgcggact tctggatctc
     7141 ccccaacagg ctgtcctaca ggcaatgtag ctcccaagtg gctgggggtc tgggcattgg
     7201 gtgcagtggg tcgagcagcc cctggagcgg ctccttcccg aagcctcatg tctaccaccc
     7261 cccccaaggg gggttccttc cccgggaagt cattatagta ctgatggtca ggtggctctt
     7321 cctcctcctc atcccatgct gagccatcaa agccagccat cctgtcatga ggggtgacca
     7381 gtttgggtgg gttcctgagg tattgtttga agcgcaactc gaaggcctgg ccaatggtgc
     7441 tgatgacatc ctgggcaagc ccttcgggac actccagaat gtggcaggct ctctgattca
     7501 cagggtcttt ggcaacatag gcgacatact cggctgtgtc cggatccccg ccggatgcaa
     7561 atgagataga ttgcatgtgg tggttggcga tgatctgttt gcagtctgcg gccatgaggt
     7621 tgaggctgct ggtggagacg gtgagagtga ttggcattcc agcaaattt
//
