LOCUS       sequence-46640-e         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Onder TT, Kara N, Cherry A, Sinha AU, Zhu N, Bernt KM, Cahan P, 
            Marcarci BO, Unternaehrer J, Gupta PB, Lander ES, Armstrong SA, 
            Daley GQ
  TITLE     Chromatin-modifying enzymes as modulators of reprogramming.
  JOURNAL   Nature. 2012 Mar 4;483(7391):598-602. doi: 10.1038/nature10953.
  PUBMED    22388813
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-03-4- 4"; volume: "483"; issue: "7391"; pages:
            "598-602"
COMMENT     Sequence Label: pSMP-SUZ12_1
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..52
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     ncRNA           138..152
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           180..306
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(399..418)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(400..418)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(472..495)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
       61 taggcgccgg aattagatct ctcgagaagg tatattgctg ttgacagtga gcgcgcagtt
      121 atgctcttaa tgctttagtg aagccacaga tgtaaagcat taagagcata actgcttgcc
      181 tactgcctcg gaattcaagg ggctacttta ggagcaatta tcttgtttac taaaactgaa
      241 taccttgcta tctctttgat acatttttac aaagctgaat taaaatggta taaattaaat
      301 cacttttttc aattggaaga ctaatgcggc cggccagtgt gcctttgtct ggtgccgggg
      361 tgttcttgga ctggtctggt gtctggtcag cttgcgggcc ctatagtgag tcgtattacc
      421 taggacgcgt tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct
      481 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc
      541 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct
      601 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga
      661 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg
      721 aagcgggtag g
//
