LOCUS       sequence-466400-       10501 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  IGF1R-ADAR2dd(E488Q, T501A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10501)
  AUTHORS   Zhang X, Mille-Fragoso LS, Kaseniit KE, Lee AP, Zhang M, Call CC, Hu
            Y, Xie Y, Gao XJ
  TITLE     Post-transcriptional modular synthetic receptors.
  JOURNAL   Nat Chem Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w.
  PUBMED    40155716
REFERENCE   2  (bases 1 to 10501)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w."
COMMENT     Sequence Label: XZ038 CMV-TO-IGF1R-ADAR2(DD-E488Q-T501A) (FLP-IN)
FEATURES             Location/Qualifiers
     source          1..10501
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(130..154)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    164..182
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    185..203
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(205..408)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(234..254)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        409..788
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     955..974
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1049..1153
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1154..1222,1223..2014)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1372..1391)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2185..2773
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2674..2693
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2927..2944
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3061..3082
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3097..3102,3103..3120,3121..3127)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3135..3151
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3140..3162
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3159..3175
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3159..3175
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    3224..3345
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3235..3254)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3289..3308
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(3475..4497)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             complement(3475..4497)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     protein_bind    complement(4505..4552)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     4684..4703
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    4875..5099
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     5088..5105
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(5161..5190)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gtcggggacc ctcctccgga gccagacttc atggtggcga agcttaagtt taaacgctag
       61 agtccggagg ctggatcggt cccggtgtct tctatggagg tcaaaacagc gtggatggcg
      121 tctccaggcg atctgacggt tcactaaacg agctcgtcga cgatctctat cactgatagg
      181 gagatctcta tcactgatag ggagagctct gcttatatag acctcccacc gtacacgcct
      241 accgcccatt tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt
      301 ttggtgccaa aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga
      361 gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc
      421 gatgactaat acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc
      481 ataatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg
      541 atacacttga tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca
      601 atggaaagtc cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg
      661 ggggtcgttg ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc
      721 catatatggg ctatgaacta atgaccccgt aattgattac tattaataac tagtcaataa
      781 tcaatgtcaa cgcgtatatc tggcccgtac gaagcagcgc aaaacgccta accctaagca
      841 gattcttcat gcaattgtcg gtcaagcctt gccttgttgt agcttaaatt ttgctcgcgc
      901 actactcagc gacctccaac acacaagcag ggagcagata ctggcttaac tatgcggcat
      961 cagagcagat tgtactgaga gtgcaccata ggggatcggg agatctcccg atccgtcgac
     1021 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     1081 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     1141 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     1201 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     1261 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     1321 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     1381 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     1441 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     1501 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     1561 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     1621 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     1681 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     1741 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     1801 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     1861 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     1921 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     1981 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     2041 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     2101 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     2161 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     2221 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     2281 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     2341 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     2401 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     2461 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     2521 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     2581 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     2641 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     2701 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     2761 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     2821 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     2881 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     2941 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     3001 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     3061 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     3121 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     3181 ttacgccaag ctctagctag aggtcgacgg tatacagaca tgataagata cattgatgag
     3241 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     3301 gctattgctt tatttgtaac cattataagc tgcaataaac aagttggggt gggcgaagaa
     3361 ctccagcatg agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc
     3421 gaagcccaac ctttcataga aggcggcggt ggaatcgaaa tctcgtagta cgtgctattc
     3481 ctttgccctc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta cttctacaca
     3541 gccatcggtc cagacggccg cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc
     3601 tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc
     3661 gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg cccggagccg
     3721 cggcgatcct gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct gctccataca
     3781 agccaaccac ggcctccaga agaagatgtt ggcgacctcg tattgggaat ccccgaacat
     3841 cgcctcgctc cagtcaatga ccgctgttat gcggccattg tccgtcagga cattgttgga
     3901 gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag tcctcggccc aaagcatcag
     3961 ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg tccatcacag tttgccagtg
     4021 atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt attgaccgat
     4081 tccttgcggt ccgaatgggc cgaacccgct cgtctggcta agatcggccg cagcgatcgc
     4141 atccatggcc tccgcgaccg gctgcagaac agcgggcagt tcggtttcag gcaggtcttg
     4201 caacgtgaca ccctgtgcac ggcgggagat gcaataggtc aggctctcgc tgaattcccc
     4261 aatgtcaagc acttccggaa tcgggagcgc ggccgatgca aagtgccgat aaacataacg
     4321 atctttgtag aaaccatcgg cgcagctatt tacccgcagg acatatccac gccctcctac
     4381 atcgaagctg aaagcacgag attcttcgcc ctccgagagc tgcatcaggt cggagacgct
     4441 gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg gtgagttcag gctttttggc
     4501 caaggaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tctaggtacg
     4561 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     4621 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     4681 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     4741 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ggggataccc
     4801 cctagagccc cagctggttc tttccgcctc agaagccata gagcccaccg catccccaga
     4861 acatcgatag atctccatag agcccaccgc atccccagca tgcctgctat tgtcttccca
     4921 atcctccccc ttgctgtcct gccccacccc accccccaga atagaatgac acctactcag
     4981 acaatgcgat gcaatttcct cattttatta ggaaaggaca gtgggagtgg caccttccag
     5041 ggtcaaggaa ggcacggggg aggggcaaac aacagatggc tggcaactag aaggcacagt
     5101 cgaggctgat cagcgggttt aaactcaatg atgattatga tgatggtcga cggcgctatt
     5161 cagatcctct tctgagatga gtttttgttc gggccctcct cgagcggctc agggcgtgag
     5221 tgagaactgg tcctgctcgg tgggcttctc cacccaggcc cccagccccg ccttgatgaa
     5281 ggctgtgaac agacgcgcct tggcggcctg gtactccttt gccgccagct tggactcatg
     5341 gtacacgttg ggtttggtaa tcttggagcg tagtaagtgg gagggaacct tgccgtgcac
     5401 acgcatccag cgacagtaca acgcgtgctt acacaggcgg gacgcgcggc ccagctcatc
     5461 cttcccagtc gtggcgttga tgacctcaat agcggagtcg cctaccgtcc agttgacact
     5521 gaagttgggg gccttccctg gctgccgtgc ttctgcattg ctgatgccac tgagcaaagg
     5581 cttgttgagg gtgtagagag gtggcaggtc ctctatgttg gagatccgct ggtacatggc
     5641 cctggaaagg tggtccccgt ggtaaaggct gcccaggatg atgctcgaga agtaaatggg
     5701 ctccacgaaa atgctgagca gggatccctg gatgcccacc acgttccagc gtgcaatctt
     5761 gtcactgcag gacatggtga gcagccgctc cccttgcagc accccgtccc acgcttggat
     5821 gctcgcattg gagcgcactg gaatcgtccc ctgaccagac tctattttgg tccgtagctg
     5881 tcctcttgct ttacgatttg ggtgtctatc tgctggttct tccaggattg gctcatgtgg
     5941 tgagaagatt ctggcatctc cacagggaga ggtgctgatg tacagatgaa actggacatt
     6001 ctccttcagc ctaaaccccc ctcgctctga tttctgaaag atggatcttt tttgatcatc
     6061 tttgttattt aagtaaagct caagttgtgt ataaagaaat ctgagcaagg atctccgaga
     6121 tattatttct gcatggcagt catttaatgc aaggccacga tcactcatgt attcaccatt
     6181 aatacatttt gttcctgtag aaacacttat caccttggca tctttaacat ctgtgcctgt
     6241 tgtcatgacg actccagcca gcacttttct gcgagcgtga ggggaggaga agttgtcggt
     6301 caggtcacca aacttaccca ggaccaggcg tgagacagcg tcagctaaaa cctgcggtaa
     6361 atgcagctga agaccctcac tgggaatagg ctggcgagat ggcgtttgat ccaagtgcaa
     6421 agcaccggta ccgcaggtcg aagactgggg cagcggcaag gcccgctcgt tcttgcggcc
     6481 cccgttcatg tgggcgtaag gctgtctctc gtcgaagctg gcgcggagga ccagcacccc
     6541 agggccggga ccgttctcgg ccttgtgtcc tgagtgtctg tcgggcagtg gcagggagga
     6601 cgaggaggcc gaggggtcca gggggacgct ctccatgttc tctggctcca ggtccagctc
     6661 ctccggctcg ggcagcttgt tctcctcgct gtagtagaag gagacctccc ggaagccagg
     6721 ctccatctcc tctttgatgc tgctgatgat ctccaggaag gaaggcctca tcttggggtt
     6781 atactgccag cacatgcgca tcagttcaaa cagcatgtca ggacagttgt ctggcttgtc
     6841 cagaaggccg ccctccatga cgaagcgaag gacttgctcg ttggacaagc cctggtaggg
     6901 ctgctcggcc agtgtggcga tctcccagag gacgaccccg aaggaccaga cgtccgagta
     6961 agtggtgaag actccatcct tgagggactc aggagacatc cagcgcacgg gcagcagccc
     7021 tttccctcct ttccggtaat agtctgtctc atagatatct cgcgtcatac caaaatctcc
     7081 gattttgact gtgaaatctt cggctaccat gcaattccgg gcagcaaggt ctctgtggac
     7141 gaacttattg gcgttgaggt atgccatgcc gtctgcaatc tctccggcca tctgaatcat
     7201 cttgctcagg cttggaggtg ctaggactgg attattctcc atttctggcc tcagagaccg
     7261 gagataactt ttgagatcgc cccgtgtcat cagttccatg atgaccagtg ttggctggcc
     7321 ttgggacacc acacccagca atcgcaccac atggtgacaa ttgaactcct tcatcacaga
     7381 agcttcgttg agaaactcaa tcctctcacg catgcttgcg gcctcgttca ctgttttaat
     7441 ggccactctg gtttcaggtt catctttcac cacacccttg gcaactcctt catagaccat
     7501 cccaaacgac ccctgcccaa gttcccggct catggtgatc ttctcccgag ccacctccca
     7561 ctcatcagga acgtacacat cagcagcgct gaagtactcc gggttcacag aggcatacag
     7621 cactccattc cccagcctgc tgttatttct ctttctatgg aagacgtaca gcataatcac
     7681 caaccctccc acgatcaaca ggacagcgac gggcagagcg atgatcagat ggatgaagtt
     7741 ttcatatcct gttttggcct ggacatagaa gaacacagga tctgtccacg acccattccc
     7801 agagagagat gtggcctgaa tccgggctgt gtagttcccc gggtttagcc ggtttagctt
     7861 ggcccctcca tacttcctgt attcctgtct ggacacacat tctcgctgat cctcaacttg
     7921 tgatccgtat tttatttcat acattagaat caatccattg ggattctcag gttccggcca
     7981 ctttaaaaag atggagtttt caggccttgg ctcccaggtc actggcccag gaatgtcatc
     8041 tgctccttct gcgggcatag tccttgcaaa gacgaagttg gaggcgctgc agcccagctt
     8101 ctcagcctcg tggttgcagc tgtggatatc gatgcggtac aatgtgaaag gccgaaggtt
     8161 agaaatgaca gttctctcct tgttatccac tctgctctca aagaaagggt actctgtctc
     8221 cagctcttcc gggtcagtga tgttgtaggt gtctgcggcc gtggtgttcc tgcttcggct
     8281 ggacatggtg gtgttggcca cttgcatgac atctctccgc ttcctttcag gtctgggcac
     8341 gaagatggag ttgtgcagga aattctcaaa gactttgcgg tattcagcct cctccttctc
     8401 ggcctgcttc tcggcttcag ttttggggca ggcgcagcaa ggccctttct ccccaccaca
     8461 cacctcagtc ttggggttct ctgtgacctc ctcaatgtcg atggtgccgt cggcatactt
     8521 cctgatgggg attttgtctt tggagcagta attgtgccgg taaaggtagc cgtcctgagg
     8581 ctgccgctgc cagcgcacaa tgtagtaact caggttgccg ttgggcagag agggagggtt
     8641 ccacttcacg attaactgag aagaggagtt cgatgctgaa agaacgtcca agggaatgga
     8701 aggaactgaa gcattggtgc gaatgtacaa gatctcactc ttggccccac ggatatggtc
     8761 gttctccacc atggtgaggg tcacagcctt gacgtaaacg gcgtactgag tccagggctt
     8821 cagcccatgt agtaagatgc cgggctccac gtccttgttg ggcgggaggt ccacgtccac
     8881 catgttccag ctgttggagc cgcaggcatc ctgcccatca tactctgtga cattcttaaa
     8941 gggtgcttcc ttgtagtaaa cggtgaagct gatgagatcc ctgtagtcag ggggccggta
     9001 ccggtgccag gttatgatga tgcgattctt cgacgtggtg gtggaggtga aatgcaggac
     9061 gtcactttca caggaggctc tctccccgtt gttcctggtg tttatgtccc ctttgctttg
     9121 gcgcccttta gtccccgtca cttcctccat gcggtaaatt tcggaaacac ataatttggg
     9181 attgaaagca aagtacattt tccctgcttt gatggtcagg ttgcggtggt cccagtccca
     9241 cagttgctgc aagttctggt tgtcgaggac gtagaaggag taattccctt ctagctgctc
     9301 ctctcctagg atgaggcgaa ggttttttag gaaggacaag gagaccaagg catgagaatg
     9361 gcggatcttc acgtagcccg tcaccacctc gatgagcccc atgaagttct ccagctctga
     9421 agcaatgtta ttcccccgtc ggatgttaat gagcaaattg cccttgaaga tggtgcatcc
     9481 ttggagcatc tgagcagaag taacagaatc aatggtcttt gttttctttt cttcctcaca
     9541 gaccttcggg caaggacctt cacaagggat gcagtacatg ctctggctgc cgttgcggat
     9601 gaagcccgag gggcactcct gcatgcactc gccgtcgtgg atcacaaacc cctcggagtc
     9661 gctgctctcg gcgctgagga tgttggcgca gaagtcacgg tccacacagc gccagccctc
     9721 aaacctgtag gtgttgggcg ggcaggcagg cacacagaca ccggcatagt agtagtggcg
     9781 gcaagctaca caggccgtgt cgttgtcagg cgcgctgcag ctgcccaggc actcggggtg
     9841 gcagcactca ttgttctcgg tgcacgcccg cttcccacac gtgcttgggc acattttctg
     9901 gcagcggttt gtggtccagc agcggtagtt gtactcattg ttgatggtgg tcttctcaca
     9961 catcggcttc tcctccatgg tccctggaca caggtcccca cattcctttg ggggcttatt
    10021 ccccacaatg tagttattgg acaccgcatc caggatcagg gaccagtcca cagtggagag
    10081 gtaacagagg tcagcatttt tctcaatcct gatggccccc cgagtaatgt tcctcaggtt
    10141 gtaaagccca atatccttga gattggtcat ctcgaagatg accagggcgt agttgtagaa
    10201 gagtttccag ccgcggatga ccgtgaggtt ggggaagagg tctccgaggc tctcgaggcc
    10261 agccactcgg aacagcagca agtactcggt aatgaccgtg agcttgggga agcggtagct
    10321 gcggtagtcc tcggccttgg agatgagcag gatgtggagg tagccctcga tcaccgtgca
    10381 gttctccagg cgcttcagct gctgatagtc gttgcggatg tcgatgcctg gcccgcagat
    10441 ttctccactc gtcggccaga gcgagagcgc ggcggagaga aacaggagcc cccacagcga
    10501 g
//
