LOCUS       sequence-466401-        7594 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CCR6-ADAR2dd(E488Q, T501A) with a short 4 amino acid linker and no 
            extra V2 tail.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7594)
  AUTHORS   Zhang X, Mille-Fragoso LS, Kaseniit KE, Lee AP, Zhang M, Call CC, Hu
            Y, Xie Y, Gao XJ
  TITLE     Post-transcriptional modular synthetic receptors.
  JOURNAL   Nat Chem Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w.
  PUBMED    40155716
REFERENCE   2  (bases 1 to 7594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w."
COMMENT     Sequence Label: LSM104
            CMV-TO-AVPR2-(noExtraV2)-ADAR2(DD-E488Q...
FEATURES             Location/Qualifiers
     source          1..7594
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(832..856)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    866..884
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    887..905
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(907..1110)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(936..956)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1111..1490
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     1657..1676
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1751..1855
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1856..1924,1925..2716)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2074..2093)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2887..3475
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3376..3395
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3629..3646
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3763..3784
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3799..3804,3805..3822,3823..3829)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3837..3853
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3842..3864
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3861..3877
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3861..3877
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    3926..4047
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3937..3956)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3991..4010
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(4177..5199)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             complement(4177..5199)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     protein_bind    complement(5207..5254)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     5386..5405
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    5577..5801
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     5790..5807
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(5863..5892)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gtgggagcaa cgaacaccat gagagcaatc cacgtcacgt aggtccttct gccccatggc
       61 tcagcgaagc aagcccagca atcggtcacg ccagatccgc cctccacatt tctctgtgcg
      121 aaaataaaca actggggcaa gctcagaagc agggaaaaag cccacgcaac cagaactggc
      181 ctgttccaat gggctccgga accgtgccta tacgccagca tggggcgaca gatagccctg
      241 tgtctgtcca aggtcatagc aagtatcata tatgaactgg catacattcc caccatctgc
      301 aggtatttca cggcccgaca cagtgcgtct ggcccccgga atctatcggt ggctttccaa
      361 gccagttgag ggagcacctg aaacagcgcc acggccaggt ctgcaagaca gagatggccg
      421 ataaagacat gaataggcgc ccagtggcct cggcggcctc gtcgcgccag ggcggccagc
      481 accagaccat tgctcaacgc gacggcaacg aaaacgatgg agagcaaggc gagctccgct
      541 cttgcaagaa gtgggtcccg ggtatccaga ggcctctcct gtgagctgtt ggagggaaga
      601 cttggaagag atggatgtcc gggcacggcg cttgtggtag aggccatgag catatcgatg
      661 ctggcgtcat catcgtcctt gtagtcggcg aataccaggc agaagatgta gctcagggcg
      721 atgatcgtct tcatggtggc gaagcttaag tttaaacgct agagtccgga ggctggatcg
      781 gtcccggtgt cttctatgga ggtcaaaaca gcgtggatgg cgtctccagg cgatctgacg
      841 gttcactaaa cgagctcgtc gacgatctct atcactgata gggagatctc tatcactgat
      901 agggagagct ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa
      961 tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact
     1021 cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg
     1081 cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat
     1141 gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca
     1201 tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc
     1261 caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg
     1321 cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca
     1381 gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg ggctatgaac
     1441 taatgacccc gtaattgatt actattaata actagtcaat aatcaatgtc aacgcgtata
     1501 tctggcccgt acgaagcagc gcaaaacgcc taaccctaag cagattcttc atgcaattgt
     1561 cggtcaagcc ttgccttgtt gtagcttaaa ttttgctcgc gcactactca gcgacctcca
     1621 acacacaagc agggagcaga tactggctta actatgcggc atcagagcag attgtactga
     1681 gagtgcacca taggggatcg ggagatctcc cgatccgtcg acgtcaggtg gcacttttcg
     1741 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     1801 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     1861 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     1921 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     1981 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     2041 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     2101 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     2161 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     2221 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     2281 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     2341 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     2401 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     2461 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     2521 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     2581 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     2641 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     2701 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     2761 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     2821 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     2881 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     2941 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     3001 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     3061 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     3121 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     3181 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     3241 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     3301 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     3361 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     3421 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     3481 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     3541 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     3601 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     3661 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     3721 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     3781 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     3841 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agctctagct
     3901 agaggtcgac ggtatacaga catgataaga tacattgatg agtttggaca aaccacaact
     3961 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     4021 accattataa gctgcaataa acaagttggg gtgggcgaag aactccagca tgagatcccc
     4081 gcgctggagg atcatccagc cggcgtcccg gaaaacgatt ccgaagccca acctttcata
     4141 gaaggcggcg gtggaatcga aatctcgtag tacgtgctat tcctttgccc tcggacgagt
     4201 gctggggcgt cggtttccac tatcggcgag tacttctaca cagccatcgg tccagacggc
     4261 cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg gctccggatc ggacgattgc
     4321 gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca agctctgata
     4381 gagttggtca agaccaatgc ggagcatata cgcccggagc cgcggcgatc ctgcaagctc
     4441 cggatgcctc cgctcgaagt agcgcgtctg ctgctccata caagccaacc acggcctcca
     4501 gaagaagatg ttggcgacct cgtattggga atccccgaac atcgcctcgc tccagtcaat
     4561 gaccgctgtt atgcggccat tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac
     4621 gaggtgccgg acttcggggc agtcctcggc ccaaagcatc agctcatcga gagcctgcgc
     4681 gacggacgca ctgacggtgt cgtccatcac agtttgccag tgatacacat ggggatcagc
     4741 aatcgcgcat atgaaatcac gccatgtagt gtattgaccg attccttgcg gtccgaatgg
     4801 gccgaacccg ctcgtctggc taagatcggc cgcagcgatc gcatccatgg cctccgcgac
     4861 cggctgcaga acagcgggca gttcggtttc aggcaggtct tgcaacgtga caccctgtgc
     4921 acggcgggag atgcaatagg tcaggctctc gctgaattcc ccaatgtcaa gcacttccgg
     4981 aatcgggagc gcggccgatg caaagtgccg ataaacataa cgatctttgt agaaaccatc
     5041 ggcgcagcta tttacccgca ggacatatcc acgccctcct acatcgaagc tgaaagcacg
     5101 agattcttcg ccctccgaga gctgcatcag gtcggagacg ctgtcgaact tttcgatcag
     5161 aaacttctcg acagacgtcg cggtgagttc aggctttttg gccaaggaag ttcctatact
     5221 ttctagagaa taggaacttc ggaataggaa cttctaggta cgtgaaccat caccctaatc
     5281 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     5341 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     5401 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     5461 cgccgcgctt aatgcgccgc tacagggcgc gtggggatac cccctagagc cccagctggt
     5521 tctttccgcc tcagaagcca tagagcccac cgcatcccca gaacatcgat agatctccat
     5581 agagcccacc gcatccccag catgcctgct attgtcttcc caatcctccc ccttgctgtc
     5641 ctgccccacc ccacccccca gaatagaatg acacctactc agacaatgcg atgcaatttc
     5701 ctcattttat taggaaagga cagtgggagt ggcaccttcc agggtcaagg aaggcacggg
     5761 ggaggggcaa acaacagatg gctggcaact agaaggcaca gtcgaggctg atcagcgggt
     5821 ttaaactcaa tgatgattat gatgatggtc gacggcgcta ttcagatcct cttctgagat
     5881 gagtttttgt tcgggccctc ctcgagcggc tcagggcgtg agtgagaact ggtcctgctc
     5941 ggtgggcttc tccacccagg cccccagccc cgccttgatg aaggctgtga acagacgcgc
     6001 cttggcggcc tggtactcct ttgccgccag cttggactca tggtacacgt tgggtttggt
     6061 aatcttggag cgtagtaagt gggagggaac cttgccgtgc acacgcatcc agcgacagta
     6121 caacgcgtgc ttacacaggc gggacgcgcg gcccagctca tccttcccag tcgtggcgtt
     6181 gatgacctca atagcggagt cgcctaccgt ccagttgaca ctgaagttgg gggccttccc
     6241 tggctgccgt gcttctgcat tgctgatgcc actgagcaaa ggcttgttga gggtgtagag
     6301 aggtggcagg tcctctatgt tggagatccg ctggtacatg gccctggaaa ggtggtcccc
     6361 gtggtaaagg ctgcccagga tgatgctcga gaagtaaatg ggctccacga aaatgctgag
     6421 cagggatccc tggatgccca ccacgttcca gcgtgcaatc ttgtcactgc aggacatggt
     6481 gagcagccgc tccccttgca gcaccccgtc ccacgcttgg atgctcgcat tggagcgcac
     6541 tggaatcgtc ccctgaccag actctatttt ggtccgtagc tgtcctcttg ctttacgatt
     6601 tgggtgtcta tctgctggtt cttccaggat tggctcatgt ggtgagaaga ttctggcatc
     6661 tccacaggga gaggtgctga tgtacagatg aaactggaca ttctccttca gcctaaaccc
     6721 ccctcgctct gatttctgaa agatggatct tttttgatca tctttgttat ttaagtaaag
     6781 ctcaagttgt gtataaagaa atctgagcaa ggatctccga gatattattt ctgcatggca
     6841 gtcatttaat gcaaggccac gatcactcat gtattcacca ttaatacatt ttgttcctgt
     6901 agaaacactt atcaccttgg catctttaac atctgtgcct gttgtcatga cgactccagc
     6961 cagcactttt ctgcgagcgt gaggggagga gaagttgtcg gtcaggtcac caaacttacc
     7021 caggaccagg cgtgagacag cgtcagctaa aacctgcggt aaatgcagct gaagaccctc
     7081 actgggaata ggctggcgag atggcgtttg atccaagtgc aaagcaccgg tacccgatga
     7141 agtgtccttg gccagggagg agctggcggt ggtgcaggac tcatcttggg gacccaggct
     7201 gggtggggtg cgtccccggg cgcaacagag aagactccgc agctcggagc taacactgga
     7261 ggagaaagag gcgtaaatcc aaggattggt acagctattc agtgaagcga gaagcatgag
     7321 cagaacgaat ggagcacctt ccaggggtgc ttcggggtcc caggccgccc agagctgcac
     7381 caaaaaaaac ggggcccagc aaagaacgta aacgacgacg atgaccagtg tcatgcgtac
     7441 ggtcttagct accgcagctg atacgtgggc gccttcgcct ggagagccag tccttcgacc
     7501 ccgtcttctt ccaccagggc gctcgcttgg cccaggaacc aaggaggcgt gtatttctct
     7561 gaatatcagg acctggcatg cggcgatccc cagg
//
