LOCUS       sequence-466404-        7424 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  IVT template for LIDAR reporter mRNA; mCherry as marker, EGFP as 
            output.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7424)
  AUTHORS   Zhang X, Mille-Fragoso LS, Kaseniit KE, Lee AP, Zhang M, Call CC, Hu
            Y, Xie Y, Gao XJ
  TITLE     Post-transcriptional modular synthetic receptors.
  JOURNAL   Nat Chem Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w.
  PUBMED    40155716
REFERENCE   2  (bases 1 to 7424)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w."
COMMENT     Sequence Label: XZ073 (IVT) CMV-TO-T7-LIDARsensor-hybridUTR3
FEATURES             Location/Qualifiers
     source          1..7424
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(129..839,840..842))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             complement(849..914)
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             complement(1083..1148)
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             complement(1194..1901)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1469..1488)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1738..1756
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      1898..1907
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2059..2078)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2060..2078)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2188..2212)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2222..2240
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2243..2261
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(2263..2466)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2292..2312)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2467..2846
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     3013..3032
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        3107..3211
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3212..3280,3281..4072)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3430..3449)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4243..4831
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4732..4751
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4985..5002
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5119..5140
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5155..5160,5161..5178,5179..5185)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5193..5209
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5198..5220
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5217..5233
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5217..5233
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    5282..5403
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5293..5312)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5347..5366
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(5533..6555)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             complement(5533..6555)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     protein_bind    complement(6563..6610)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     6742..6761
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    6933..7157
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     7146..7163
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(7219..7248)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gcagaggtgg tgagtggggc aggtggaggt gggagcatac ctgggacccg aggtcggggg
       61 agactcgggg tacccaggac gggaaagggg cagctagcat tgcgtgcatg cagtaccagc
      121 tcgagctact tatacagttc gtccatcccg agtgttatcc cagccgcagt gacgaactca
      181 agaaggacca tgtgatcccg tttttcgttt gggtccttgc taagagctga ctgagtagac
      241 aaatagtgat tatcagggag aagtactgga ccatccccta tcggcgtgtt ctgttggtaa
      301 tggtccgcaa gctggacaga gccgtcctca atattgtgtc ggattttaaa gtttaccttg
      361 atgccgtttt tctgtttgtc cgccatgata tagacattat gagaattata gttgtattcg
      421 agcttgtggc ccagtatatt gccatcctcc ttaaaatcta tgcccttcag ttcgatccgg
      481 ttcacgagag tgtcaccctc gaattttacc tcggctctag ttttgtagtt tccgtcgtct
      541 ttaaaaaata tcgttcgctc ctgtacgtaa ccttcaggca tagcggattt aaaaaaatcg
      601 tgctgtttca tatggtctgg ataccggcta aaacactgaa caccatatgt cagggtggtg
      661 acgagagtag gccacggcac tggcaatttt ccggtggtac atatgaactt cagcgtaagt
      721 ttcccatatg tcgcgtcccc ctcaccctca ccactaactg agaatttatg cccattaacg
      781 tccccgtcca attcgacgag gatcggaaca acgcccgtaa aaagttcctc gccctttgag
      841 acactcgagg gtcctggatt agattccaca tcacctgcga gtttgagtag atcgaagttg
      901 agtgtttgct ttactggggc tacgatcttt tgcttatgcc gtgcctcact agtaccggtc
      961 gtctgggtag gtgggatact atggtacgct gatgctgtac cagacagacc ctgatggtgt
     1021 ctgagacgta ccctgatggt gtacgttact attccaccca cctaaacgga ggatccggta
     1081 ccgggaccgg ggttggactc gacgtctccc gccagcttca gaaggtcaaa attcaaagtc
     1141 tgtttcaccg gagccacaat tttctgtttg tgtctggctt ccaacgcgtc gctcttgtac
     1201 agctcgtcca tgccgccggt ggagtggcgg ccctcggcgc gttcgtactg ttccacgatg
     1261 gtgtagtcct cgttgtggga ggtgatgtcc aacttgatgt tgacgttgta ggcgccgggc
     1321 agctgcacgg gcttcttggc cttgtaggtg gtcttgacct cagcgtcgta gtggccgccg
     1381 tccttcagct tcagcctctg cttgatctcg cccttcaggg cgccgtcctc ggggtacatc
     1441 cgctcggagg aggcctccca gcccatggtc ttcttctgca ttacggggcc gtcggagggg
     1501 aagttggtgc cgcgcagctt caccttgtag atgaactcgc cgtcctgcag ggaggagtcc
     1561 tgggtcacgg tcaccacgcc gccgtcctcg aagttcatca cgcgctccca cttgaagccc
     1621 tcggggaagg acagcttcaa gtagtcggga atgtcggcgg ggtgcttcac gtaggccttg
     1681 gagccgtaca tgaactgagg ggacaggatg tcccaggcga agggcagggg gccacccttg
     1741 gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc ggccctcgcc ctcgccctcg
     1801 atctcgaact cgtggccgtt cacggagccc tccatgtgca ccttgaagcg catgaactcc
     1861 ttgatgatgg ccatgttatc ctcctcgccc ttgctcacca tggtggcgaa gctttatcgt
     1921 tcgtaaatgg tgaaaatttt cagaaaattg cttttgcttt aaaagaaatg atttaaattg
     1981 ctgcaataga agtagaatgc ttgattgctt gagattcgtt tgttttgtat atgttgtgtt
     2041 gagatttgtt atttttctcc ctatagtgag tcgtattagc ggccgcggat ctctagcgag
     2101 cttaagttta aacgctagag tccggaggct ggatcggtcc cggtgtcttc tatggaggtc
     2161 aaaacagcgt ggatggcgtc tccaggcgat ctgacggttc actaaacgag ctcgtcgacg
     2221 atctctatca ctgataggga gatctctatc actgataggg agagctctgc ttatatagac
     2281 ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt tacgacattt
     2341 tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa tggggtggag
     2401 acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact gccaaaaccg
     2461 catcaccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag gaaagtccca
     2521 taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga cgtcaatagg
     2581 gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt accgtaaata
     2641 ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa catacgtcat
     2701 tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag
     2761 ttatgtaacg cggaactcca tatatgggct atgaactaat gaccccgtaa ttgattacta
     2821 ttaataacta gtcaataatc aatgtcaacg cgtatatctg gcccgtacga agcagcgcaa
     2881 aacgcctaac cctaagcaga ttcttcatgc aattgtcggt caagccttgc cttgttgtag
     2941 cttaaatttt gctcgcgcac tactcagcga cctccaacac acaagcaggg agcagatact
     3001 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatagg ggatcgggag
     3061 atctcccgat ccgtcgacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     3121 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     3181 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     3241 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     3301 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     3361 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     3421 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     3481 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     3541 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     3601 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     3661 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     3721 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     3781 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     3841 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     3901 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     3961 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     4021 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     4081 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     4141 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     4201 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     4261 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     4321 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     4381 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     4441 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     4501 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     4561 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     4621 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     4681 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     4741 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     4801 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     4861 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     4921 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     4981 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     5041 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     5101 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     5161 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     5221 aaacagctat gaccatgatt acgccaagct ctagctagag gtcgacggta tacagacatg
     5281 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     5341 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     5401 gttggggtgg gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc
     5461 gtcccggaaa acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc
     5521 tcgtagtacg tgctattcct ttgccctcgg acgagtgctg gggcgtcggt ttccactatc
     5581 ggcgagtact tctacacagc catcggtcca gacggccgcg cttctgcggg cgatttgtgt
     5641 acgcccgaca gtcccggctc cggatcggac gattgcgtcg catcgaccct gcgcccaagc
     5701 tgcatcatcg aaattgccgt caaccaagct ctgatagagt tggtcaagac caatgcggag
     5761 catatacgcc cggagccgcg gcgatcctgc aagctccgga tgcctccgct cgaagtagcg
     5821 cgtctgctgc tccatacaag ccaaccacgg cctccagaag aagatgttgg cgacctcgta
     5881 ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc ggccattgtc
     5941 cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg tgccggactt cggggcagtc
     6001 ctcggcccaa agcatcagct catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc
     6061 catcacagtt tgccagtgat acacatgggg atcagcaatc gcgcatatga aatcacgcca
     6121 tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg aacccgctcg tctggctaag
     6181 atcggccgca gcgatcgcat ccatggcctc cgcgaccggc tgcagaacag cgggcagttc
     6241 ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc aataggtcag
     6301 gctctcgctg aattccccaa tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa
     6361 gtgccgataa acataacgat ctttgtagaa accatcggcg cagctattta cccgcaggac
     6421 atatccacgc cctcctacat cgaagctgaa agcacgagat tcttcgccct ccgagagctg
     6481 catcaggtcg gagacgctgt cgaacttttc gatcagaaac ttctcgacag acgtcgcggt
     6541 gagttcaggc tttttggcca aggaagttcc tatactttct agagaatagg aacttcggaa
     6601 taggaacttc taggtacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     6661 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     6721 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     6781 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     6841 gggcgcgtgg ggataccccc tagagcccca gctggttctt tccgcctcag aagccataga
     6901 gcccaccgca tccccagaac atcgatagat ctccatagag cccaccgcat ccccagcatg
     6961 cctgctattg tcttcccaat cctccccctt gctgtcctgc cccaccccac cccccagaat
     7021 agaatgacac ctactcagac aatgcgatgc aatttcctca ttttattagg aaaggacagt
     7081 gggagtggca ccttccaggg tcaaggaagg cacgggggag gggcaaacaa cagatggctg
     7141 gcaactagaa ggcacagtcg aggctgatca gcgggtttaa actcaatgat gattatgatg
     7201 atggtcgacg gcgctattca gatcctcttc tgagatgagt ttttgttcgg gcccgctagc
     7261 tccagggtgt ggctggcacg aaattgacca accctggggt tagtatagct tagttaaact
     7321 ttcgtttatt gctaaaggtt aatcactgct gtttcccgtg ggggtgtggc taggctaagc
     7381 gttttgagct gcattgctgc gtgcttggga ggtgtctgga acta
//
