LOCUS       sequence-466405-        7603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CCR6-ADAR2dd(E488Q, T501A) with a short 4 amino acid linker and no 
            V2 tail.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7603)
  AUTHORS   Zhang X, Mille-Fragoso LS, Kaseniit KE, Lee AP, Zhang M, Call CC, Hu
            Y, Xie Y, Gao XJ
  TITLE     Post-transcriptional modular synthetic receptors.
  JOURNAL   Nat Chem Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w.
  PUBMED    40155716
REFERENCE   2  (bases 1 to 7603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w."
COMMENT     Sequence Label: XZ031 CMV-TO-CCR6-ADAR2dd(E488Q, T501A)
FEATURES             Location/Qualifiers
     source          1..7603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1621..1650
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(1706..1723)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1712..1936
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2108..2127)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    2259..2306
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             2314..3336
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             2314..3336
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    3466..3587
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3503..3522)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3557..3576
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3636..3652)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3636..3652)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3649..3671)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3660..3676
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3684..3690,3691..3708,3709..3714))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3729..3750
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3867..3884)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4038..4626)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4118..4137)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4797..5588,5589..5657))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5420..5439
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5658..5762)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5837..5856)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6023..6402
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6403..6606
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6557..6577
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    6608..6626
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6629..6647
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     6657..6681
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 cgacacaagg ccatccgggt aatcatcgct gttgtgctgg tatttctggc ttgccagatc
       61 cctcataata tggtccttct ggtaacggca gccaacctgg gcaaaatgaa tcgctcatgt
      121 cagtcagaaa agcttatcgg gtacaccaaa acggttacgg aggttctggc gttcctgcat
      181 tgttgcctta accctgtgct gtacgcattt ataggtcaga agtttcgaaa ctacttcctg
      241 aagattctca aagacctctg gtgtgtaaga agaaagtata aaagtagtgg attttcatgc
      301 gcaggacgct actccgaaaa catttccaga cagaccagtg agacggccga taacgataac
      361 gcctcctcct ttaccatggg taccggtgct ttgcacttgg atcaaacgcc atctcgccag
      421 cctattccca gtgagggtct tcagctgcat ttaccgcagg ttttagctga cgctgtctca
      481 cgcctggtcc tgggtaagtt tggtgacctg accgacaact tctcctcccc tcacgctcgc
      541 agaaaagtgc tggctggagt cgtcatgaca acaggcacag atgttaaaga tgccaaggtg
      601 ataagtgttt ctacaggaac aaaatgtatt aatggtgaat acatgagtga tcgtggcctt
      661 gcattaaatg actgccatgc agaaataata tctcggagat ccttgctcag atttctttat
      721 acacaacttg agctttactt aaataacaaa gatgatcaaa aaagatccat ctttcagaaa
      781 tcagagcgag gggggtttag gctgaaggag aatgtccagt ttcatctgta catcagcacc
      841 tctccctgtg gagatgccag aatcttctca ccacatgagc caatcctgga agaaccagca
      901 gatagacacc caaatcgtaa agcaagagga cagctacgga ccaaaataga gtctggtcag
      961 gggacgattc cagtgcgctc caatgcgagc atccaagcgt gggacggggt gctgcaaggg
     1021 gagcggctgc tcaccatgtc ctgcagtgac aagattgcac gctggaacgt ggtgggcatc
     1081 cagggatccc tgctcagcat tttcgtggag cccatttact tctcgagcat catcctgggc
     1141 agcctttacc acggggacca cctttccagg gccatgtacc agcggatctc caacatagag
     1201 gacctgccac ctctctacac cctcaacaag cctttgctca gtggcatcag caatgcagaa
     1261 gcacggcagc cagggaaggc ccccaacttc agtgtcaact ggacggtagg cgactccgct
     1321 attgaggtca tcaacgccac gactgggaag gatgagctgg gccgcgcgtc ccgcctgtgt
     1381 aagcacgcgt tgtactgtcg ctggatgcgt gtgcacggca aggttccctc ccacttacta
     1441 cgctccaaga ttaccaaacc caacgtgtac catgagtcca agctggcggc aaaggagtac
     1501 caggccgcca aggcgcgtct gttcacagcc ttcatcaagg cggggctggg ggcctgggtg
     1561 gagaagccca ccgagcagga ccagttctca ctcacgccct gagccgctcg aggagggccc
     1621 gaacaaaaac tcatctcaga agaggatctg aatagcgccg tcgaccatca tcataatcat
     1681 cattgagttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     1741 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     1801 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     1861 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     1921 gcggtgggct ctatggagat ctatcgatgt tctggggatg cggtgggctc tatggcttct
     1981 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     2041 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     2101 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     2161 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     2221 cccaaaaaac ttgattaggg tgatggttca cgtacctaga agttcctatt ccgaagttcc
     2281 tattctctag aaagtatagg aacttccttg gccaaaaagc ctgaactcac cgcgacgtct
     2341 gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag
     2401 ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta
     2461 aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc
     2521 gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc
     2581 atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct
     2641 gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg
     2701 agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc
     2761 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc
     2821 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa
     2881 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc
     2941 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc
     3001 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag
     3061 cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt
     3121 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag
     3181 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc
     3241 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac
     3301 cgacgcccca gcactcgtcc gagggcaaag gaatagcacg tactacgaga tttcgattcc
     3361 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     3421 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     3481 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     3541 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     3601 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     3661 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     3721 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     3781 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     3841 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     3901 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     3961 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     4021 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     4081 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     4141 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     4201 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     4261 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     4321 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     4381 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     4441 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     4501 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     4561 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     4621 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     4681 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     4741 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     4801 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     4861 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     4921 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     4981 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     5041 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     5101 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     5161 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     5221 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     5281 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     5341 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     5401 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     5461 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     5521 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     5581 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     5641 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     5701 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     5761 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc
     5821 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
     5881 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
     5941 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
     6001 gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt
     6061 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     6121 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     6181 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     6241 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     6301 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     6361 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     6421 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     6481 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     6541 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     6601 agagctctcc ctatcagtga tagagatctc cctatcagtg atagagatcg tcgacgagct
     6661 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
     6721 agacaccggg accgatccag cctccggact ctagcgttta aacttaagct tcgccaccat
     6781 gaagacgatc atcgccctga gctacatctt ctgcctggta ttcgccgact acaaggacga
     6841 tgatgacgcc agcatcgata tgagcggaga gagcatgaac ttcagtgatg tgttcgattc
     6901 cagtgaggac tacttcgtgt ccgtcaatac atcctactac agcgtagatt ctgagatgct
     6961 gctttgctcc ctgcaggagg tgcgccagtt ctcccgactc ttcgttccca tcgcgtattc
     7021 actcatctgc gtctttggcc tcctggggaa tatcttggta gtcatcacat ttgcttttta
     7081 taaaaaggcc agatccatga cagacgtcta cctgcttaat atggccattg cagacatcct
     7141 gttcgtcctg acgctcccat tctgggctgt gagtcacgct accggggcct gggtgttcag
     7201 taatgcaacc tgcaagctgt tgaagggaat ttatgccatc aactttaatt gcggtatgct
     7261 gctgctgact tgcataagta tggacagata catcgccata gtacaggcaa caaaatcctt
     7321 cagactgagg tctcgaacgc tccctaggtc taaaatcatt tgtcttgttg tatggggctt
     7381 gagcgtgatt atttccagca gtactttcgt gtttaaccag aagtacaaca cgcagggcag
     7441 cgatgtgtgt gagcccaagt accagacggt atctgagcct atcaggtgga agctgttgat
     7501 gctgggtttg gaattgctgt tcggcttttt tatcccactc atgtttatga ttttttgtta
     7561 taccttcatc gtgaagaccc tggtccaggc acaaaactct aag
//
