LOCUS       sequence-46641-e         606 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Onder TT, Kara N, Cherry A, Sinha AU, Zhu N, Bernt KM, Cahan P, 
            Marcarci BO, Unternaehrer J, Gupta PB, Lander ES, Armstrong SA, 
            Daley GQ
  TITLE     Chromatin-modifying enzymes as modulators of reprogramming.
  JOURNAL   Nature. 2012 Mar 4;483(7391):598-602. doi: 10.1038/nature10953.
  PUBMED    22388813
REFERENCE   2  (bases 1 to 606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-03-4- 4"; volume: "483"; issue: "7391"; pages:
            "598-602"
COMMENT     Sequence Label: pSMP-SUZ12_2
FEATURES             Location/Qualifiers
     source          1..606
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     37..53
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     ncRNA           139..153
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           181..307
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(401..420)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(402..420)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(474..497)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ttgaacctcc tcgttcgacc ccgccatcga tcctcccttt atccagccct cactccttct
       61 ctaggcgccg gaattagatc tctcgagaag gtatattgct gttgacagtg agcgcgcatg
      121 catgacttta atcttatagt gaagccacag atgtataaga ttaaagtcat gcatgcttgc
      181 ctactgcctc ggaattcaag gggctacttt aggagcaatt atcttgttta ctaaaactga
      241 ataccttgct atctctttga tacattttta caaagctgaa ttaaaatggt ataaattaaa
      301 tcactttttt caattggaag actaatgcgg ccggccaagg taatattttc gctgggcggt
      361 gtgcggtccc ttgtctccgc ttttgcttgg cggtggtggg ccctatagtg agtcgtatta
      421 cctaggacgc gttctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg
      481 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
      541 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
      601 ctactc
//
