LOCUS       sequence-466416-       10006 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutively expresses eGFP with puromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10006)
  AUTHORS   Rheinemann L, Downhour DM, Davenport KA, McKeown AN, Sundquist WI, 
            Elde NC
  TITLE     Recurrent evolution of an inhibitor of ESCRT-dependent virus budding
            and LINE-1 retrotransposition in primates.
  JOURNAL   Curr Biol. 2022 Feb 25. pii: S0960-9822(22)00240-8. doi: 
            10.1016/j.cub.2022.02.018.
  PUBMED    35245459
REFERENCE   2  (bases 1 to 10006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2022 Feb 25. pii: S0960-9822(22)00240-8. doi: 
            10.1016/j.cub.2022.02.018."
COMMENT     Sequence Label: pCEP4-puroeGFP
FEATURES             Location/Qualifiers
     source          1..10006
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      645..654
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(651..653,654..656,657..1370)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(651..653,654..656,657..1367)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(696..717)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(957..976)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1304..1325
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    1398..1532
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1422..1441)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1476..1495
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1675..1697)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      2257..4046
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(6207..6225)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6293..6397
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6398..6466,6467..7258)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6616..6635)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7429..8017
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7918..7937
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        8448..8593
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     primer_bind     9131..9151
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(9261..9280)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9305..9353
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatctctag aagctgggta ccgcgggccc gggatccacc ggtcgccacc atggtgagca
      661 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
      721 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
      781 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
      841 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
      901 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
      961 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     1021 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     1081 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     1141 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     1201 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     1261 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     1321 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccgct
     1381 cgaggccggc aaggccggat ccagacatga taagatacat tgatgagttt ggacaaacca
     1441 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     1501 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     1561 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     1621 gtatggctga ttatgatccg gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg
     1681 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     1741 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgaggtcgac
     1801 tctagaggat cgatccccgc cccggacgaa ctaaacctga ctacgacatc tctgcccctt
     1861 cttcgcgggg cagtgcatgt aatcccttca gttggttggt acaacttgcc aactgggccc
     1921 tgttccacat gtgacacggg gggggaccaa acacaaaggg gttctctgac tgtagttgac
     1981 atccttataa atggatgtgc acatttgcca acactgagtg gctttcatcc tggagcagac
     2041 tttgcagtct gtggactgca acacaacatt gcctttatgt gtaactcttg gctgaagctc
     2101 ttacaccaat gctgggggac atgtacctcc caggggccca ggaagactac gggaggctac
     2161 accaacgtca atcagagggg cctgtgtagc taccgataag cggaccctca agagggcatt
     2221 agcaatagtg tttataaggc ccccttgtta accctaaacg ggtagcatat gcttcccggg
     2281 tagtagtata tactatccag actaacccta attcaatagc atatgttacc caacgggaag
     2341 catatgctat cgaattaggg ttagtaaaag ggtcctaagg aacagcgata tctcccaccc
     2401 catgagctgt cacggtttta tttacatggg gtcaggattc cacgagggta gtgaaccatt
     2461 ttagtcacaa gggcagtggc tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc
     2521 gcctgcttct tcattctcct tcgtttagct aatagaataa ctgctgagtt gtgaacagta
     2581 aggtgtatgt gaggtgctcg aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg
     2641 acggggggtt cagtggtggc attgtgctat gacaccaata taaccctcac aaaccccttg
     2701 ggcaataaat actagtgtag gaatgaaaca ttctgaatat ctttaacaat agaaatccat
     2761 ggggtgggga caagccgtaa agactggatg tccatctcac acgaatttat ggctatgggc
     2821 aacacataat cctagtgcaa tatgatactg gggttattaa gatgtgtccc aggcagggac
     2881 caagacaggt gaaccatgtt gttacactct atttgtaaca aggggaaaga gagtggacgc
     2941 cgacagcagc ggactccact ggttgtctct aacacccccg aaaattaaac ggggctccac
     3001 gccaatgggg cccataaaca aagacaagtg gccactcttt tttttgaaat tgtggagtgg
     3061 gggcacgcgt cagcccccac acgccgccct gcggttttgg actgtaaaat aagggtgtaa
     3121 taacttggct gattgtaacc ccgctaacca ctgcggtcaa accacttgcc cacaaaacca
     3181 ctaatggcac cccggggaat acctgcataa gtaggtgggc gggccaagat aggggcgcga
     3241 ttgctgcgat ctggaggaca aattacacac acttgcgcct gagcgccaag cacagggttg
     3301 ttggtcctca tattcacgag gtcgctgaga gcacggtggg ctaatgttgc catgggtagc
     3361 atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg ggtagcatag
     3421 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
     3481 gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
     3541 gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg ggtagcatat
     3601 gctatcctaa tagagattag ggtagtatat gctatcctaa tttatatctg ggtagcatat
     3661 actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta gcatatgcta
     3721 tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta
     3781 tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta gcataggcta
     3841 tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta
     3901 tcctaatctg tatccgggta gcatatgcta tcctcatgca tatacagtca gcatatgata
     3961 cccagtagta gagtgggagt gctatccttt gcatatgccg ccacctccca agggggcgtg
     4021 aattttcgct gcttgtcctt ttcctgctgg ttgctcccat tcttaggtga atttaaggag
     4081 gccaggctaa agccgtcgca tgtctgattg ctcaccaggt aaatgtcgct aatgttttcc
     4141 aacgcgagaa ggtgttgagc gcggagctga gtgacgtgac aacatgggta tgcccaattg
     4201 ccccatgttg ggaggacgaa aatggtgaca agacagatgg ccagaaatac accaacagca
     4261 cgcatgatgt ctactgggga tttattcttt agtgcggggg aatacacggc ttttaatacg
     4321 attgagggcg tctcctaaca agttacatca ctcctgccct tcctcaccct catctccatc
     4381 acctccttca tctccgtcat ctccgtcatc accctccgcg gcagcccctt ccaccatagg
     4441 tggaaaccag ggaggcaaat ctactccatc gtcaaagctg cacacagtca ccctgatatt
     4501 gcaggtagga gcgggctttg tcataacaag gtccttaatc gcatccttca aaacctcagc
     4561 aaatatatga gtttgtaaaa agaccatgaa ataacagaca atggactccc ttagcgggcc
     4621 aggttgtggg ccgggtccag gggccattcc aaaggggaga cgactcaatg gtgtaagacg
     4681 acattgtgga atagcaaggg cagttcctcg ccttaggttg taaagggagg tcttactacc
     4741 tccatatacg aacacaccgg cgacccaagt tccttcgtcg gtagtccttt ctacgtgact
     4801 cctagccagg agagctctta aaccttctgc aatgttctca aatttcgggt tggaacctcc
     4861 ttgaccacga tgctttccaa accaccctcc ttttttgcgc ctgcctccat caccctgacc
     4921 ccggggtcca gtgcttgggc cttctcctgg gtcatctgcg gggccctgct ctatcgctcc
     4981 cgggggcacg tcaggctcac catctgggcc accttcttgg tggtattcaa aataatcggc
     5041 ttcccctaca gggtggaaaa atggccttct acctggaggg ggcctgcgcg gtggagaccc
     5101 ggatgatgat gactgactac tgggactcct gggcctcttt tctccacgtc cacgacctct
     5161 ccccctggct ctttcacgac ttccccccct ggctctttca cgtcctctac cccggcggcc
     5221 tccactacct cctcgacccc ggcctccact acctcctcga ccccggcctc cactgcctcc
     5281 tcgaccccgg cctccacctc ctgctcctgc ccctcctgct cctgcccctc ctcctgctcc
     5341 tgcccctcct gcccctcctg ctcctgcccc tcctcctgct cctgcccctc ctcctgctcc
     5401 tgcccctcct gcccctcctg cccctcctgc ccctcctcct gctcctgccc ctcctcctgc
     5461 tcctgcccct cctgctcctg cccctcccgc tcctgctcct gctcctgttc caccgtgggt
     5521 ccctttgcag ccaatgcaac ttggacgttt ttggggtctc cggacaccat ctctatgtct
     5581 tggccctgat cctgagccgc ccggggctcc tggtcttccg cctcctcgtc ctcgtcctct
     5641 tccccgtcct cgtccatggt tatcaccccc tcttctttga ggtccactgc cgccggagcc
     5701 ttctggtcca gatgtgtctc ccttctctcc taggccattt ccaggtcctg tacctggccc
     5761 ctcgtcagac atgattcaca ctaaaagaga tcaatagaca tctttattag acgacgctca
     5821 gtgaatacag ggagtgcaga ctcctgcccc ctccaacagc ccccccaccc tcatcccctt
     5881 catggtcgct gtcagacaga tccaggtctg aaaattcccc atcctccgaa ccatcctcgt
     5941 cctcatcacc aattactcgc agcccggaaa actcccgctg aacatcctca agatttgcgt
     6001 cctgagcctc aagccaggcc tcaaattcct cgtccccctt tttgctggac ggtagggatg
     6061 gggattctcg ggacccctcc tcttcctctt caaggtcacc agacagagat gctactgggg
     6121 caacggaaga aaagctgggt gcggcctgtg aggatcagct tatcgatgat aagctgtcaa
     6181 acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     6241 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     6301 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     6361 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     6421 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     6481 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     6541 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     6601 cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca
     6661 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     6721 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     6781 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     6841 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     6901 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt
     6961 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     7021 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     7081 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     7141 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     7201 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     7261 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     7321 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     7381 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     7441 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     7501 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     7561 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     7621 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     7681 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     7741 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     7801 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     7861 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     7921 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     7981 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     8041 acggttcctg gccttttgct ggccttgaag ctgtccctga tggtcgtcat ctacctgcct
     8101 ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat
     8161 ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc
     8221 cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa
     8281 gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg
     8341 gtgaatccgt tagcgaggtg ccgccctgct tcatccccgt ggcccgttgc tcgcgtttgc
     8401 tggcggtgtc cccggaagaa atatatttgc atgtctttag ttctatgatg acacaaaccc
     8461 cgcccagcgt cttgtcattg gcgaattcga acacgcagat gcagtcgggg cggcgcggtc
     8521 cgaggtccac ttcgcatatt aaggtgacgc gtgtggcctc gaacaccgag cgaccctgca
     8581 gcgacccgct taacagcgtc aacagcgtgc cgcagatccc ggggggcaat gagatatgac
     8641 cgagacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc
     8701 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac
     8761 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     8821 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     8881 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     8941 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     9001 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     9061 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     9121 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     9181 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacggatc
     9241 gggagatggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct
     9301 atgacggcaa taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg ttcataaacg
     9361 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca
     9421 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg
     9481 gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc actggccccg tgggttaggg
     9541 acggggtccc ccatggggaa tggtttatgg ttcgtggggg ttattatttt gggcgttgcg
     9601 tggggtcagg tccacgactg gactgagcag acagacccat ggtttttgga tggcctgggc
     9661 atggaccgca tgtactggcg cgacacgaac accgggcgtc tgtggctgcc aaacaccccc
     9721 gacccccaaa aaccaccgcg cggatttctg gcgtgccaag ctagtcgacc aattctcatg
     9781 tttgacagct tatcatcgca gatccgggca acgttgttgc cattgctgca ggcgcagaac
     9841 tggtaggtat ggaagatcta tacattgaat caatattggc aattagccat attagtcatt
     9901 ggttatatag cataaatcaa tattggctat tggccattgc atacgttgta tctatatcat
     9961 aatatgtaca tttatattgg ctcatgtcca atatgaccgc catgtt
//
