LOCUS       sequence-466420-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TGP-E, a thermostable fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Anderson MR, Dargatz CJ, Banerjee T, DeVore NM
  TITLE     Green, yellow, or cyan? Introduction of color change mutations into 
            a green thermostable fluorescent protein and characterization during
            an introduction to biochemistry lab course.
  JOURNAL   Biochem Mol Biol Educ. 2024 Sep-Oct;52(5):549-558. doi: 
            10.1002/bmb.21841. Epub 2024 Jun 8.
  PUBMED    38850239
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem Mol
            Biol Educ. 2024 Sep-Oct"; volume: "52(5)"; pages: "549-558. doi: 
            10.1002/bmb.21841. Epub 2024 Jun 8"
COMMENT     Sequence Label: pETCK3 TGP-E
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             29..51
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             752..793
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 ggggggggaa cattccctct agaataattt tgtttaactt taagaaggag atatacatat
       61 gggcgcgcat gcctcagtaa ttaaaccgga aatgaaaatt aaattgcgta tggaaggtgc
      121 cgttaacggc cataaatttg taattgaagg agaaggaata ggcaaaccat acgaaggaac
      181 ccagaccctg gatttaaccg tagaagaagg cgcacctctc cctttctcgt acgacatcct
      241 caccccagcc ttcgaatacg gcaatcgcgc tttcaccaaa tacccagaag atattccaga
      301 ctattttaaa caagcattcc ccgaaggcta ttcttgggaa cgctctatga cctatgaaga
      361 tcaaggaatt tgtatcgcta cctccgacat tactatggaa ggagactgtt ttttttatga
      421 aattcgcttt gatggaacta acttcccccc gaacggccct gtaatgcaaa agaagacctt
      481 aaaatgggaa cctagcaccg aaaaaatgta tgtagaagac ggagttctta agggtgacgt
      541 agaaatggca cttctgctcg aaggaggtgg acactaccgc tgcgatttta aaaccactta
      601 taaagccaaa aaagatgttc gtcttccaga tgcacacgag gtggaccacc gcattgaaat
      661 cctgagccac gataaagatt ataataaagt tagactctat gaacacgccg aagcccgcta
      721 ttctggcgga ggcagcggag gcggtgctag cggcaaacca atcccaaacc cactgctggg
      781 cctggatagt actccccatc accatcacca ttacgcggcc gcttgcatcc caccaccccc
      841 ccccccacaa ccccaacacc accacccccc ccccctaaat ccgcctctcc
//
