LOCUS       sequence-466422-         721 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TGP-E, a thermostable fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Anderson MR, Dargatz CJ, Banerjee T, DeVore NM
  TITLE     Green, yellow, or cyan? Introduction of color change mutations into 
            a green thermostable fluorescent protein and characterization during
            an introduction to biochemistry lab course.
  JOURNAL   Biochem Mol Biol Educ. 2024 Sep-Oct;52(5):549-558. doi: 
            10.1002/bmb.21841. Epub 2024 Jun 8.
  PUBMED    38850239
REFERENCE   2  (bases 1 to 721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem Mol
            Biol Educ. 2024 Sep-Oct"; volume: "52(5)"; pages: "549-558. doi: 
            10.1002/bmb.21841. Epub 2024 Jun 8"
COMMENT     Sequence Label: pETCK3 TGP-E
FEATURES             Location/Qualifiers
     source          1..721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(44..61)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(71..88)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(108..125)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(126..167)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 gggcccctcc cttctttcgg gctttgttag cagccggatc tcagtggtgg tggtggtggt
       61 gctcgagtta gtggtggtgg tggtggtggg atccaagcgg ccgcctaatg gtgatggtga
      121 tggtgagtac tatccaggcc cagcagtggg tttgggattg gtttgccgct agcaccgcct
      181 ccgctgcctc cgccagaata gcgggcttcg gcgtgttcat agagtctaac tttattataa
      241 tctttatcgt ggctcaggat ttcaatgcgg tggtccacct cgtgtgcatc tggaagacga
      301 acatcttttt tggctttata agtggtttta aaatcgcagc ggtagtgtcc acctccttcg
      361 agcagaagtg ccatttctac gtcaccctta agaactccgt cttctacata cattttttcg
      421 gtgctaggtt cccattttaa ggtcttcttt tgcattacag ggccgttcgg ggggaagtta
      481 gttccatcaa agcgaatttc ataaaaaaaa cagtctcctt ccatagtaat gtcggaggta
      541 gcgatacaaa ttccttgatc ttcataggtc atagagcgtt cccaagaata gccttcgggg
      601 aatgcttgtt taaaatagtc tggaatatct tctgggtatt tggtgaaagc gcgattgccg
      661 tattcgaagg ctggggtgag gatgtcgtac gacaagacgc aaaggtgctc cttcttctac
      721 c
//
