LOCUS       sequence-466423-        3978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient expression of N-terminally FLAG-tagged human WDR59-SEC13 
            ""single chain"" in the GATOR2 complex.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Valenstein ML, Wranik M, Lalgudi PV, Linde-Garelli KY, Choi Y, 
            Chivukula RR, Sabatini DM, Rogala KB
  TITLE     Structural basis for the dynamic regulation of mTORC1 by amino 
            acids.
  JOURNAL   Nature. 2025 Aug 20. doi: 10.1038/s41586-025-09428-7.
  PUBMED    40836086
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2025 Aug 20. doi: 10.1038/s41586-025-09428-7."
COMMENT     Sequence Label: FLAG-sc-WDR59-SEC13
FEATURES             Location/Qualifiers
     source          1..3978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..33
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atggaaatgg actacaagga tgacgatgac aagggtggtg gtggtggtgc gtcgacgatg
       61 gcggcgcgat ggagcagcga aaacgtggtt gtagagttcc gtgactccca ggcaactgcg
      121 atgtctgtgg actgtcttgg gcagcatgca gtgctttctg gccgcagatt cttatacatc
      181 gtcaatctag atgccccttt cgaaggtcac cgaaagatct ctcgccagag caaatgggac
      241 attggagctg tgcagtggaa tcctcatgac agctttgcac actattttgc ggcttcgagt
      301 aaccaacgag tagaccttta caagtggaaa gacggcagtg gggaagttgg cacaacctta
      361 caaggccaca ctcgtgtcat cagcgacttg gactgggcgg tgtttgagcc tgacctcctg
      421 gttaccagct ctgtggacac ctacatctac atttgggata tcaaagacac aaggaaacct
      481 actgttgcac tgtctgctgt tgcgggtgcc tcccaggtca aatggaataa aaaaaatgct
      541 aactgccttg ccaccagcca tgacggcgat gtgcggatat gggataagag gaaacccagt
      601 acagcagtgg aatatctagc cgcccacctc tccaaaatcc atggcctgga ctggcaccca
      661 gacagcgagc acattcttgc tacctccagt caagacaatt ctgtgaagtt ctgggattac
      721 cgccagcctc ggaaatacct caatattctt ccttgccagg tgcctgtctg gaaggccaga
      781 tacacacctt tcagcaatgg attggtgact gtgatggttc cccagctgcg gagggaaaac
      841 agccttctcc tgtggaatgt ctttgacttg aacaccccag tccacacctt cgtggggcat
      901 gatgatgtgg tcctggagtt ccagtggagg aagcagaagg aagggtccaa ggactatcaa
      961 ctggtgacgt ggtcccggga tcagaccttg agaatgtggc gggtggattc ccagatgcag
     1021 aggctttgtg caaatgacat attagatggt gttgatgagt tcattgagag tatttccctt
     1081 ctgccggaac ctgagaagac cctgcacact gaagatacag atcaccagca cactgcaagc
     1141 catggggagg aagaagccct aaaagaagat ccccctagaa atctcctgga agagaggaaa
     1201 tcagatcaac tggggctgcc tcagaccttg cagcaggaat tctccctgat caatgtgcaa
     1261 atccggaatg tcaatgtgga gatggatgcg gcagacagga gctgcacagt gtctgtgcac
     1321 tgcagcaacc atcgtgtcaa gatgctggtg aagttccctg cacagtaccc aaacaacgcc
     1381 gccccttcct tccagtttat taaccccaca accatcacat ccaccatgaa agctaagctg
     1441 ctgaagatcc tgaaggacac agccctgcag aaagtgaagc gtggccagag ctgcctggag
     1501 ccctgcctgc gccagctcgt ctcctgcctt gagtcctttg tgaaccagga agacagcgct
     1561 tccagcaacc cgtttgcact ccccaactct gtcactcccc ccttaccgac gtttgcgcgg
     1621 gtgaccacgg cttacgggtc gtaccaggac gccaacattc cctttcctag gacttctggg
     1681 gccaggttct gcggagcagg ttacctggta tatttcacaa ggcccatgac aatgcatcgg
     1741 gcggtgtctc ccacagagcc tactccgaga tctctctcag ccttgtctgc ttatcacact
     1801 ggcttgatcg cgcccatgaa gatccgcaca gaggcccctg ggaaccttcg tttatacagt
     1861 gggagcccca ctcgcagcga gaaagagcag gtctccatca gctccttcta ctacaaggag
     1921 cggaaatcaa gacgatggaa aagtaagcgt gagggatcag actctggcaa tcgacagatc
     1981 aaggctgctg ggaaagtcat catccaggat ggcagcggaa gtggtatggt gtcagtaatt
     2041 aacactgtgg atacctccca tgaggacatg attcacgacg cccagatgga ctactatggc
     2101 acccgcctgg caacctgctc atcagacagg tccgtcaaaa tctttgatgt gcgcaatgga
     2161 gggcagatcc ttatcgccga cctcaggggt catgagggtc ctgtgtggca agtggcctgg
     2221 gctcacccca tgtacggcaa catcctggca tcgtgctcct atgaccggaa agtcattatc
     2281 tggagagagg aaaacggcac ctgggagaag agccacgagc atgcgggaca cgactcctca
     2341 gtgaactcgg tgtgctgggc cccccatgac tacggcctga tcctggcctg tgggagctcg
     2401 gatggggcca tctccctgct gacttacacc ggggaaggcc aatgggaagt aaagaagatc
     2461 aacaacgctc acaccattgg ctgcaatgcc gtcagctggg cccctgctgt tgtacctgga
     2521 agcctcatag accacccatc ggggcagaaa cccaattaca tcaagaggtt tgcatcaggt
     2581 ggctgtgaca acctcatcaa gctgtggaag gaggaggagg acggccagtg gaaggaggag
     2641 cagaagctag aagcgcacag tgactgggtt cgagatgtgg cctgggcccc ctccatcggc
     2701 ctgcccacca gcaccatcgc cagctgctcc caggatggtc gtgtgttcat ttggacctgt
     2761 gatgatgcct caagcaatac gtggtcccct aaattgttgc acaagttcaa cgatgtggtg
     2821 tggcatgtga gctggtccat cacagccaac atcctggctg tctctggtgg agacaataag
     2881 gtgaccctgt ggaaggagtc agttgatggg cagtgggtgt gcatcagtga tgtcaacaag
     2941 ggccagggct ccgtatcagc atcagtgaca gagggccagc agaacgagca gggtagcggc
     3001 agtgggattg cttgcctcct gcctgttcac aaatcgctgg gagagctgta catattgaat
     3061 gtgaatgata ttcaggaaac atgtcagaag aatgccgcct ctgccttgct cgttggaaga
     3121 aaggatcttg tccaggtttg gtcgctggct acggtagcta cagatctttg ccttggtccg
     3181 aaatctgacc cagatttgga aacaccctgg gctcgacatc catttgggcg gcagctgctg
     3241 gagtccctgt tggctcacta ttgccggctc cgggatgttc agacactggc gatgctctgt
     3301 agcgtgtttg aagcccagtc tcggcctcag gggctaccaa acccctttgg gccttttcct
     3361 aaccgttctt ctaatcttgt ggtgtcccat agtcgatatc ctagctttac ctcttctggt
     3421 tcctgctcca gtatgtcaga cccagggctc aacactggcg gctggaacat agcgggaaga
     3481 gaggcagagc acttgtcctc cccttgggga gaatcctcac cagaagagct ccgctttggg
     3541 agtctgacct acagtgatcc ccgtgagcga gaacgcgacc agcatgataa aaataaaagg
     3601 ctcctggacc ccgccaatac ccagcaattt gatgacttta agaaatgcta tggggaaatc
     3661 ctctaccgtt ggggtctgag agagaagcga gctgaagtgt tgaagtttgt ctcctgtcct
     3721 cctgaccctc acaaagggat cgagttcggc gtgtactgca gccactgccg gagtgaggtc
     3781 cgtggcacgc agtgtgccat ctgcaaaggc ttcacgttcc agtgtgccat ctgtcacgtg
     3841 gctgtgcggg gatcgtccaa tttctgcctg acctgtgggc acggtggcca caccagccac
     3901 atgatggagt ggtttcggac ccaggaggtg tgtcccaccg ggtgtgggtg ccactgcctg
     3961 cttgaaagca ctttctga
//
