LOCUS       sequence-466426-        9164 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral overexpression vector for NADK2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9164)
  AUTHORS   Flickinger KM, Mellado Fritz CA, Huggler KS, Wade GM, Chang GR, Fox 
            KC, Simcox JA, Cantor JR
  TITLE     Cytosolic NADK is conditionally essential for folate-dependent 
            nucleotide synthesis.
  JOURNAL   Nat Metab. 2025 May 2. doi: 10.1038/s42255-025-01272-3.
  PUBMED    40316835
REFERENCE   2  (bases 1 to 9164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Metab. 
            2025 May 2. doi: 10.1038/s42255-025-01272-3."
COMMENT     Sequence Label: pLJC6-NADK2-3xFLAG
FEATURES             Location/Qualifiers
     source          1..9164
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2881..4092
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3929..3948
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             5475..5540
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    5588..5705
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5754..6264
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6183..6201
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             6273..6668
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             6740..6973
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7045..7166
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7082..7101)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7136..7155
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7206..7341
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7361..7380)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7362..7380)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7390..7407)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7390..7406)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7399..7421)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7548..8003
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7635..7654)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7845..7866
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8029..8133
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8134..8202,8203..8994)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8352..8371)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcagcag
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2821 attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg agcgggatct
     2881 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
     2941 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
     3001 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
     3061 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
     3121 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
     3181 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
     3241 cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct
     3301 ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc
     3361 caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg
     3421 cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga
     3481 ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt
     3541 cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct
     3601 gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt
     3661 tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc
     3721 gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg
     3781 cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat
     3841 cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg
     3901 gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg
     3961 ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca
     4021 atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct
     4081 tttttgttag acgaagcgct agcgctaccg gtttaattaa gcccacgatg acctgttacc
     4141 gaggattcct tttgggaagc tgttgtcggg tggctggagg acgagctgcg gcactccggg
     4201 gtcccggagc cgggggacca gcagccagac ctagattggg gggcgatgga ggcgggcgga
     4261 ggcaccttgg tcaaggacag ccgcgggagc tggcggggtg cggatcaaga gccgacggag
     4321 ggttccggcc aagccgagta gtagtcgtgg cgaaaacgac gaggtacgag tttgagcagc
     4381 agcgctaccg ctatgctgag ctctccgagg aagaccttaa gcaacttctt gccttgaaag
     4441 ggtcttctta ctctggactg cttgaacgac accatataca tacaaaaaac gtagaacata
     4501 ttattgatag cttgcgcaat gaaggcattg aggtgcgatt ggttaaaaga cgagagtacg
     4561 acgaagagac agtaagatgg gccgatgcgg ttatcgctgc cggcggcgat ggaacgatgc
     4621 tcctcgcggc atctaaggta ctcgaccgac ttaagccagt cattggcgtg aacacagacc
     4681 ctgaacgaag cgaaggacat ctttgcttgc cagttcgata cacccattca tttccggagg
     4741 ctctgcaaaa attctaccgg ggtgaattcc gatggctgtg gcggcaacgg atacgcctgt
     4801 acctggaagg aaccggcatt aaccctgtcc ctgtcgacct tcacgagcaa cagctgtctc
     4861 ttaaccagca caatcgagca ttgaatattg aacgagccca tgatgagaga tctgaagcga
     4921 gtggaccaca gcttttgcca gtgagggctt tgaacgaagt gttcattggt gaatcactct
     4981 ctagtcgggc gagttactac gagatctcag tggacgacgg accatgggag aaacagaaga
     5041 gctcaggatt gaatctgtgc acggggactg gatctaaagc ttggagcttt aatattaaca
     5101 gggtcgccac tcaggctgtg gaggatgtac ttaacatcgc aaagcgacag ggtaacctta
     5161 gtctcccgct caaccgcgaa ctggtggaaa aagtaaccaa cgaatataat gaatccctcc
     5221 tgtactcccc agaagagcct aagatcctct tttcaattcg cgagccgatt gcaaaccggg
     5281 tttttagcag cagcaggcag agatgtttct cctcaaaagt atgtgttcga agccgatgct
     5341 gggatgcctg tatggtagtc gatgggggta caagctttga atttaacgac ggagcaattg
     5401 cctccatgat gatcaataag gaagacgaac ttcggactgt actgctggaa cagggaggaa
     5461 gtgcggccgc aggcgactac aaagaccatg acggtgatta taaagatcat gacattgatt
     5521 acaaggatga cgatgacaag taataagaat tctcgacctc gagacaaatg gcagtattca
     5581 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     5641 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     5701 attttcgggt ttattacagg gacagcagag atccactttg gccgcggctc gagggggttg
     5761 gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt
     5821 ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac gtccgttcgc
     5881 agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg
     5941 cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc
     6001 gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc agcgcgccga
     6061 ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag cagcggccgg
     6121 gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg
     6181 cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc cctcgttgac
     6241 cgaatcaccg acctctctcc ccagggggat ccatggttcc tttgtctcaa gaagaatcca
     6301 ccctcattga aagagcaacg gctacaatca acagcatccc catctctgaa gactacagcg
     6361 tcgccagcgc agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt
     6421 ttactggggg accttgtgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg
     6481 gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg
     6541 gacggtgccg acaggtgctt ctcgatctgc atcctgggat caaagccata gtgaaggaca
     6601 gtgatggaca gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg
     6661 agggctaagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     6721 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     6781 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     6841 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     6901 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     6961 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     7021 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     7081 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     7141 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     7201 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     7261 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     7321 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
     7381 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     7441 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     7501 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     7561 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     7621 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     7681 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     7741 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     7801 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     7861 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     7921 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     7981 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     8041 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     8101 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     8161 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     8221 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     8281 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     8341 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     8401 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     8461 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     8521 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     8581 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     8641 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     8701 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     8761 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     8821 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     8881 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     8941 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     9001 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     9061 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     9121 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttc
//
